Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.01.50
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7b7b053d342f4d31b34c209411cc6927",
"name": "M1_20170603_1447_nk1_fast.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:02:17"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 23.9684
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66465e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 56 mean_circ: 0.923779
Ran for 1550 frames Cell Events: 84 mean_circ: 0.926428
Ran for 2050 frames Cell Events: 112 mean_circ: 0.926428
Ran for 3550 frames Cell Events: 221 mean_circ: 0.925364
Ran for 7050 frames Cell Events: 439 mean_circ: 0.925007
Ran for 7550 frames Cell Events: 467 mean_circ: 0.924554
Ran for 12550 frames Cell Events: 843 mean_circ: 0.925806
Ran for 13550 frames Cell Events: 910 mean_circ: 0.926073
Ran for 14550 frames Cell Events: 956 mean_circ: 0.925809
Ran for 16550 frames Cell Events: 1060 mean_circ: 0.925485
Ran for 19550 frames Cell Events: 1242 mean_circ: 0.925345
Ran for 22050 frames Cell Events: 1390 mean_circ: 0.926121
Ran for 23050 frames Cell Events: 1471 mean_circ: 0.92599
Ran for 24050 frames Cell Events: 1558 mean_circ: 0.925861
Ran for 25550 frames Cell Events: 1668 mean_circ: 0.926463
Ran for 27550 frames Cell Events: 1833 mean_circ: 0.92663
Ran for 28050 frames Cell Events: 1871 mean_circ: 0.926639
Ran for 30050 frames Cell Events: 1985 mean_circ: 0.926748
Ran for 30550 frames Cell Events: 2017 mean_circ: 0.926737
Ran for 31550 frames Cell Events: 2082 mean_circ: 0.926924
Ran for 34050 frames Cell Events: 2238 mean_circ: 0.926811
Ran for 34550 frames Cell Events: 2270 mean_circ: 0.926853
Ran for 35050 frames Cell Events: 2309 mean_circ: 0.926849
Ran for 37050 frames Cell Events: 2496 mean_circ: 0.926898
Ran for 38050 frames Cell Events: 2546 mean_circ: 0.927256
Ran for 38550 frames Cell Events: 2586 mean_circ: 0.927368
Ran for 39050 frames Cell Events: 2621 mean_circ: 0.927416
Ran for 39550 frames Cell Events: 2653 mean_circ: 0.927418
Ran for 40050 frames Cell Events: 2695 mean_circ: 0.927418
Ran for 43550 frames Cell Events: 2949 mean_circ: 0.927277
Ran for 44050 frames Cell Events: 2972 mean_circ: 0.927212
Ran for 45050 frames Cell Events: 3037 mean_circ: 0.927333
Ran for 46550 frames Cell Events: 3150 mean_circ: 0.927422
Ran for 47050 frames Cell Events: 3179 mean_circ: 0.9275
Ran for 48050 frames Cell Events: 3248 mean_circ: 0.927773
Ran for 48550 frames Cell Events: 3277 mean_circ: 0.927744
Ran for 49050 frames Cell Events: 3312 mean_circ: 0.927787
Ran for 49550 frames Cell Events: 3347 mean_circ: 0.927834
Ran for 51050 frames Cell Events: 3454 mean_circ: 0.927895
Ran for 51550 frames Cell Events: 3495 mean_circ: 0.927856
Ran for 52050 frames Cell Events: 3522 mean_circ: 0.927811
Ran for 52550 frames Cell Events: 3561 mean_circ: 0.927845
Ran for 53550 frames Cell Events: 3629 mean_circ: 0.927904
Ran for 55050 frames Cell Events: 3703 mean_circ: 0.927964
Ran for 55550 frames Cell Events: 3741 mean_circ: 0.927914
Ran for 56550 frames Cell Events: 3819 mean_circ: 0.928019
Ran for 57050 frames Cell Events: 3850 mean_circ: 0.927995
Ran for 58050 frames Cell Events: 3929 mean_circ: 0.927987
Ran for 59050 frames Cell Events: 4024 mean_circ: 0.928079
Ran for 59550 frames Cell Events: 4038 mean_circ: 0.928217
Ran for 61050 frames Cell Events: 4171 mean_circ: 0.928149
Ran for 62050 frames Cell Events: 4231 mean_circ: 0.927978
Ran for 63050 frames Cell Events: 4315 mean_circ: 0.927881
Ran for 64050 frames Cell Events: 4381 mean_circ: 0.927803
Ran for 64550 frames Cell Events: 4404 mean_circ: 0.927842
Ran for 65050 frames Cell Events: 4431 mean_circ: 0.927867
Ran for 69050 frames Cell Events: 4707 mean_circ: 0.927832
Ran for 71550 frames Cell Events: 4850 mean_circ: 0.927734
Ran for 72050 frames Cell Events: 4889 mean_circ: 0.927734
Ran for 74050 frames Cell Events: 5026 mean_circ: 0.927676
Ran for 76050 frames Cell Events: 5165 mean_circ: 0.927883
Ran for 78550 frames Cell Events: 5352 mean_circ: 0.927833
Ran for 79550 frames Cell Events: 5428 mean_circ: 0.92787
Ran for 80050 frames Cell Events: 5469 mean_circ: 0.927884
Ran for 81050 frames Cell Events: 5537 mean_circ: 0.92787
Ran for 82050 frames Cell Events: 5609 mean_circ: 0.927865
Ran for 82550 frames Cell Events: 5640 mean_circ: 0.927886
Ran for 85050 frames Cell Events: 5832 mean_circ: 0.927658
Ran for 87050 frames Cell Events: 5961 mean_circ: 0.927576
Ran for 89550 frames Cell Events: 6165 mean_circ: 0.927629
Ran for 90550 frames Cell Events: 6259 mean_circ: 0.927575
Ran for 92050 frames Cell Events: 6348 mean_circ: 0.927377
Ran for 93550 frames Cell Events: 6463 mean_circ: 0.927302
Ran for 94550 frames Cell Events: 6514 mean_circ: 0.927364
Ran for 96550 frames Cell Events: 6667 mean_circ: 0.92743
Ran for 99550 frames Cell Events: 6896 mean_circ: 0.927411
Ran for 100550 frames Cell Events: 6960 mean_circ: 0.927499
Ran for 101050 frames Cell Events: 6991 mean_circ: 0.927498
Ran for 101550 frames Cell Events: 7025 mean_circ: 0.927543
Ran for 102050 frames Cell Events: 7065 mean_circ: 0.927504
Ran for 103050 frames Cell Events: 7123 mean_circ: 0.927591
Ran for 104050 frames Cell Events: 7191 mean_circ: 0.927428
Ran for 104550 frames Cell Events: 7222 mean_circ: 0.927451
Ran for 106550 frames Cell Events: 7331 mean_circ: 0.927418
Ran for 108050 frames Cell Events: 7448 mean_circ: 0.927344
Ran for 109550 frames Cell Events: 7552 mean_circ: 0.927288
Ran for 110550 frames Cell Events: 7634 mean_circ: 0.92718
Ran for 111550 frames Cell Events: 7708 mean_circ: 0.927221
Ran for 113050 frames Cell Events: 7836 mean_circ: 0.927149
Ran for 113550 frames Cell Events: 7877 mean_circ: 0.927149
Ran for 114050 frames Cell Events: 7931 mean_circ: 0.927062
Ran for 116050 frames Cell Events: 8069 mean_circ: 0.926972
Ran for 118050 frames Cell Events: 8189 mean_circ: 0.926934
Ran for 119050 frames Cell Events: 8274 mean_circ: 0.926917
Ran for 119550 frames Cell Events: 8316 mean_circ: 0.926913
Ran for 120050 frames Cell Events: 8359 mean_circ: 0.926911
Ran for 120550 frames Cell Events: 8398 mean_circ: 0.926935
Ran for 123050 frames Cell Events: 8545 mean_circ: 0.926812
Ran for 124550 frames Cell Events: 8631 mean_circ: 0.92681
Ran for 125050 frames Cell Events: 8669 mean_circ: 0.926775
Ran for 127050 frames Cell Events: 8799 mean_circ: 0.926686
Ran for 128050 frames Cell Events: 8862 mean_circ: 0.926626
Ran for 129550 frames Cell Events: 8948 mean_circ: 0.926545
Ran for 130050 frames Cell Events: 8989 mean_circ: 0.926523
Ran for 132050 frames Cell Events: 9162 mean_circ: 0.92643
Ran for 132550 frames Cell Events: 9194 mean_circ: 0.926461
Ran for 133550 frames Cell Events: 9259 mean_circ: 0.926494
Ran for 134050 frames Cell Events: 9302 mean_circ: 0.926519
Ran for 134550 frames Cell Events: 9335 mean_circ: 0.926515
Ran for 135050 frames Cell Events: 9366 mean_circ: 0.926508
Ran for 135550 frames Cell Events: 9400 mean_circ: 0.92653
Ran for 136050 frames Cell Events: 9439 mean_circ: 0.926545
Ran for 138550 frames Cell Events: 9660 mean_circ: 0.926422
Ran for 142550 frames Cell Events: 9927 mean_circ: 0.926291
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 1.66472e+009
****************************************
Measurement finished
Mean Circularitiy: 0.926234
Mean Area: 653.959
Mean FL-1 intensity: 3136.01
Mean FL-2 intensity: 4017.81
Mean FL-3 intensity: 0
frames processed: 139739
Total Time: 71844.1ms
Number of Runs (new bg img) 0
Frames collected: 143504
Skipped frames: 4415
Rescued Frames: 170729
Samples DAQ: 35876619
Samples written: 71751758
Samples per Channel: 35875879
FPS: 1997.44
Samples per second: 499357
Expected samples/s: 500000
Events: 10001
Events per second: 139.204
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :231115
# 1 frames :43983
# 2 frames :2225
# 3 frames :928
# 4 frames :438
# 5 frames :212
# 6 frames :2421
# 7 frames :1025
# 8 frames :932
# 9 frames :597
# 10 frames :407
# 11 frames :267
# 12 frames :220
# 13 frames :234
# 14 frames :272
# 15 frames :222
# 16 frames :189
# 17 frames :142
# 18 frames :119
# 19 frames :96
# 20 frames :98
# 21 frames :87
# 22 frames :68
# 23 frames :66
# 24 frames :65
# 25 frames :51
# 26 frames :51
# 27 frames :38
# 28 frames :28
# 29 frames :27
# 30 frames :30
# 31 frames :20
# 32 frames :21
# 33 frames :15
# 34 frames :24
# 35 frames :22
# 36 frames :9
# 37 frames :10
# 38 frames :13
# 39 frames :16
# 40 frames :4
# 41 frames :8
# 42 frames :6
# 43 frames :7
# 44 frames :4
# 45 frames :4
# 46 frames :2
# 47 frames :5
# 48 frames :5
# 49 frames :5
# 50 frames :2
# 51 frames :4
# 52 frames :1
# 53 frames :2
# 54 frames :3
# 55 frames :4
# 56 frames :2
# 57 frames :1
# 58 frames :2
# 59 frames :5
# 60 frames :1
# 61 frames :1
# 62 frames :2
# 72 frames :1
# 73 frames :1
# 84 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 23.9684
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0