Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.07.27
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7627b8ec91ec1ac03372ef5e747038a2",
"name": "M1_20170603_1555_NK1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:07:52"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9999 (max 10000) in
'9999'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.2773
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66875e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 106 mean_circ: 0.943016
Ran for 3050 frames Cell Events: 212 mean_circ: 0.945718
Ran for 3550 frames Cell Events: 241 mean_circ: 0.945644
Ran for 4550 frames Cell Events: 305 mean_circ: 0.945481
Ran for 7050 frames Cell Events: 501 mean_circ: 0.946245
Ran for 7550 frames Cell Events: 539 mean_circ: 0.946596
Ran for 8050 frames Cell Events: 570 mean_circ: 0.946655
Ran for 9550 frames Cell Events: 664 mean_circ: 0.946505
Ran for 11550 frames Cell Events: 827 mean_circ: 0.946709
Ran for 14050 frames Cell Events: 993 mean_circ: 0.947172
Ran for 15550 frames Cell Events: 1069 mean_circ: 0.946673
Ran for 16550 frames Cell Events: 1140 mean_circ: 0.94672
Ran for 19550 frames Cell Events: 1343 mean_circ: 0.946421
Ran for 20050 frames Cell Events: 1380 mean_circ: 0.946149
Ran for 21550 frames Cell Events: 1470 mean_circ: 0.946258
Ran for 22050 frames Cell Events: 1503 mean_circ: 0.946158
Ran for 22550 frames Cell Events: 1535 mean_circ: 0.94609
Ran for 23050 frames Cell Events: 1567 mean_circ: 0.946064
Ran for 23550 frames Cell Events: 1600 mean_circ: 0.945895
Ran for 24050 frames Cell Events: 1631 mean_circ: 0.945702
Ran for 26050 frames Cell Events: 1766 mean_circ: 0.945885
Ran for 27550 frames Cell Events: 1848 mean_circ: 0.945513
Ran for 30550 frames Cell Events: 2049 mean_circ: 0.945431
Ran for 31050 frames Cell Events: 2079 mean_circ: 0.945523
Ran for 32050 frames Cell Events: 2132 mean_circ: 0.945688
Ran for 33050 frames Cell Events: 2209 mean_circ: 0.945716
Ran for 35550 frames Cell Events: 2399 mean_circ: 0.945695
Ran for 36050 frames Cell Events: 2429 mean_circ: 0.94586
Ran for 36550 frames Cell Events: 2460 mean_circ: 0.945881
Ran for 37550 frames Cell Events: 2530 mean_circ: 0.946066
Ran for 38050 frames Cell Events: 2564 mean_circ: 0.946126
Ran for 40050 frames Cell Events: 2714 mean_circ: 0.946017
Ran for 41550 frames Cell Events: 2828 mean_circ: 0.945952
Ran for 42050 frames Cell Events: 2862 mean_circ: 0.945874
Ran for 42550 frames Cell Events: 2886 mean_circ: 0.945918
Ran for 45050 frames Cell Events: 3079 mean_circ: 0.94541
Ran for 45550 frames Cell Events: 3115 mean_circ: 0.945477
Ran for 46050 frames Cell Events: 3142 mean_circ: 0.945513
Ran for 46550 frames Cell Events: 3176 mean_circ: 0.945446
Ran for 47050 frames Cell Events: 3209 mean_circ: 0.945539
Ran for 48550 frames Cell Events: 3312 mean_circ: 0.945491
Ran for 50050 frames Cell Events: 3394 mean_circ: 0.945426
Ran for 50550 frames Cell Events: 3428 mean_circ: 0.945475
Ran for 51050 frames Cell Events: 3462 mean_circ: 0.945476
Ran for 53050 frames Cell Events: 3578 mean_circ: 0.945564
Ran for 55550 frames Cell Events: 3774 mean_circ: 0.945504
Ran for 57550 frames Cell Events: 3926 mean_circ: 0.945541
Ran for 58550 frames Cell Events: 3987 mean_circ: 0.945479
Ran for 59050 frames Cell Events: 4008 mean_circ: 0.945488
Ran for 59550 frames Cell Events: 4032 mean_circ: 0.945523
Ran for 60050 frames Cell Events: 4059 mean_circ: 0.9455
Ran for 61050 frames Cell Events: 4114 mean_circ: 0.945533
Ran for 61550 frames Cell Events: 4143 mean_circ: 0.945493
Ran for 62050 frames Cell Events: 4188 mean_circ: 0.945513
Ran for 66550 frames Cell Events: 4478 mean_circ: 0.945446
Ran for 68050 frames Cell Events: 4598 mean_circ: 0.945432
Ran for 69550 frames Cell Events: 4697 mean_circ: 0.945304
Ran for 73550 frames Cell Events: 4966 mean_circ: 0.94539
Ran for 74550 frames Cell Events: 5030 mean_circ: 0.945421
Ran for 75550 frames Cell Events: 5113 mean_circ: 0.94538
Ran for 76550 frames Cell Events: 5175 mean_circ: 0.945442
Ran for 78550 frames Cell Events: 5344 mean_circ: 0.945397
Ran for 81050 frames Cell Events: 5542 mean_circ: 0.945509
Ran for 82550 frames Cell Events: 5658 mean_circ: 0.945525
Ran for 84550 frames Cell Events: 5795 mean_circ: 0.945569
Ran for 86050 frames Cell Events: 5893 mean_circ: 0.945596
Ran for 86550 frames Cell Events: 5926 mean_circ: 0.945565
Ran for 89050 frames Cell Events: 6103 mean_circ: 0.945602
Ran for 89550 frames Cell Events: 6123 mean_circ: 0.94559
Ran for 93050 frames Cell Events: 6388 mean_circ: 0.9456
Ran for 93550 frames Cell Events: 6422 mean_circ: 0.945674
Ran for 94550 frames Cell Events: 6502 mean_circ: 0.945725
Ran for 96550 frames Cell Events: 6639 mean_circ: 0.945674
Ran for 99050 frames Cell Events: 6835 mean_circ: 0.945583
Ran for 100550 frames Cell Events: 6952 mean_circ: 0.945662
Ran for 101550 frames Cell Events: 7017 mean_circ: 0.945694
Ran for 103550 frames Cell Events: 7155 mean_circ: 0.94572
Ran for 105550 frames Cell Events: 7295 mean_circ: 0.945783
Ran for 106050 frames Cell Events: 7341 mean_circ: 0.945777
Ran for 108050 frames Cell Events: 7488 mean_circ: 0.945829
Ran for 109550 frames Cell Events: 7613 mean_circ: 0.945909
Ran for 110050 frames Cell Events: 7647 mean_circ: 0.945895
Ran for 112050 frames Cell Events: 7806 mean_circ: 0.945943
Ran for 112550 frames Cell Events: 7836 mean_circ: 0.945954
Ran for 113050 frames Cell Events: 7877 mean_circ: 0.945942
Ran for 113550 frames Cell Events: 7898 mean_circ: 0.945954
Ran for 115050 frames Cell Events: 8017 mean_circ: 0.945791
Ran for 115550 frames Cell Events: 8049 mean_circ: 0.945804
Ran for 116050 frames Cell Events: 8087 mean_circ: 0.945764
Ran for 118050 frames Cell Events: 8230 mean_circ: 0.945848
Ran for 119050 frames Cell Events: 8318 mean_circ: 0.945871
Ran for 119550 frames Cell Events: 8338 mean_circ: 0.94586
Ran for 120550 frames Cell Events: 8396 mean_circ: 0.945899
Ran for 122050 frames Cell Events: 8515 mean_circ: 0.945929
Ran for 122550 frames Cell Events: 8549 mean_circ: 0.945945
Ran for 123050 frames Cell Events: 8585 mean_circ: 0.945945
Ran for 123550 frames Cell Events: 8629 mean_circ: 0.945904
Ran for 124050 frames Cell Events: 8665 mean_circ: 0.945905
Ran for 124550 frames Cell Events: 8698 mean_circ: 0.9459
Ran for 127050 frames Cell Events: 8911 mean_circ: 0.945878
Ran for 127550 frames Cell Events: 8946 mean_circ: 0.945883
Ran for 133050 frames Cell Events: 9392 mean_circ: 0.945811
Ran for 133550 frames Cell Events: 9428 mean_circ: 0.945819
Ran for 134050 frames Cell Events: 9469 mean_circ: 0.945842
Ran for 136550 frames Cell Events: 9661 mean_circ: 0.945855
Ran for 137550 frames Cell Events: 9724 mean_circ: 0.945867
Ran for 138050 frames Cell Events: 9764 mean_circ: 0.94585
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 1.66882e+009
****************************************
Measurement finished
Mean Circularitiy: 0.945874
Mean Area: 638.419
Mean FL-1 intensity: 3394.46
Mean FL-2 intensity: 3149.07
Mean FL-3 intensity: 0
frames processed: 137930
Total Time: 70883.8ms
Number of Runs (new bg img) 0
Frames collected: 141524
Skipped frames: 33236
Rescued Frames: 1181528
Samples DAQ: 35381156
Samples written: 70761850
Samples per Channel: 35380925
FPS: 1996.56
Samples per second: 499140
Expected samples/s: 500000
Events: 10000
Events per second: 141.076
jumped back (PMT): 2963
#Frames read, frequency
# 0 frames :236384
# 1 frames :34400
# 2 frames :2268
# 3 frames :904
# 4 frames :545
# 5 frames :286
# 6 frames :1375
# 7 frames :949
# 8 frames :793
# 9 frames :492
# 10 frames :593
# 11 frames :452
# 12 frames :306
# 13 frames :218
# 14 frames :270
# 15 frames :266
# 16 frames :225
# 17 frames :185
# 18 frames :137
# 19 frames :111
# 20 frames :103
# 21 frames :109
# 22 frames :102
# 23 frames :88
# 24 frames :57
# 25 frames :62
# 26 frames :68
# 27 frames :54
# 28 frames :41
# 29 frames :42
# 30 frames :43
# 31 frames :38
# 32 frames :40
# 33 frames :27
# 34 frames :31
# 35 frames :28
# 36 frames :23
# 37 frames :23
# 38 frames :10
# 39 frames :15
# 40 frames :11
# 41 frames :12
# 42 frames :16
# 43 frames :10
# 44 frames :9
# 45 frames :16
# 46 frames :5
# 47 frames :2
# 48 frames :3
# 49 frames :3
# 50 frames :6
# 51 frames :4
# 52 frames :6
# 53 frames :6
# 54 frames :3
# 55 frames :1
# 56 frames :5
# 57 frames :5
# 58 frames :6
# 59 frames :3
# 61 frames :3
# 62 frames :1
# 63 frames :2
# 64 frames :1
# 65 frames :1
# 70 frames :2
# 72 frames :1
# 73 frames :1
# 74 frames :2
# 76 frames :1
# 77 frames :1
# 87 frames :1
# 88 frames :2
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.2773
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0