Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.13.56
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "4f8293d6028f4e502eb936bc5a4ce809",
"name": "M1_20170603_1701_dsRNA_C6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:14:15"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8063 (max 8064) in '8063'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4325
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67269e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 86 mean_circ: 0.936269
Ran for 2050 frames Cell Events: 163 mean_circ: 0.938861
Ran for 4550 frames Cell Events: 362 mean_circ: 0.938987
Ran for 7550 frames Cell Events: 609 mean_circ: 0.940513
Ran for 9050 frames Cell Events: 727 mean_circ: 0.940833
Ran for 10050 frames Cell Events: 817 mean_circ: 0.939715
Ran for 10550 frames Cell Events: 858 mean_circ: 0.940135
Ran for 11050 frames Cell Events: 883 mean_circ: 0.940156
Ran for 12550 frames Cell Events: 994 mean_circ: 0.940394
Ran for 14050 frames Cell Events: 1102 mean_circ: 0.941086
Ran for 15050 frames Cell Events: 1202 mean_circ: 0.941137
Ran for 17050 frames Cell Events: 1368 mean_circ: 0.941331
Ran for 18050 frames Cell Events: 1433 mean_circ: 0.941324
Ran for 24050 frames Cell Events: 1940 mean_circ: 0.941267
Ran for 25550 frames Cell Events: 2058 mean_circ: 0.940914
Ran for 26050 frames Cell Events: 2096 mean_circ: 0.940975
Ran for 27050 frames Cell Events: 2186 mean_circ: 0.940878
Ran for 28050 frames Cell Events: 2267 mean_circ: 0.940988
Ran for 29550 frames Cell Events: 2406 mean_circ: 0.940564
Ran for 30050 frames Cell Events: 2440 mean_circ: 0.940667
Ran for 34550 frames Cell Events: 2831 mean_circ: 0.940571
Ran for 37050 frames Cell Events: 3050 mean_circ: 0.940686
Ran for 37550 frames Cell Events: 3087 mean_circ: 0.940495
Ran for 42050 frames Cell Events: 3476 mean_circ: 0.940453
Ran for 42550 frames Cell Events: 3504 mean_circ: 0.940474
Ran for 43050 frames Cell Events: 3531 mean_circ: 0.940474
Ran for 43550 frames Cell Events: 3579 mean_circ: 0.9404
Ran for 45550 frames Cell Events: 3729 mean_circ: 0.940426
Ran for 48550 frames Cell Events: 3948 mean_circ: 0.940469
Ran for 49050 frames Cell Events: 3978 mean_circ: 0.940168
Ran for 49550 frames Cell Events: 4009 mean_circ: 0.940147
Ran for 52050 frames Cell Events: 4226 mean_circ: 0.940264
Ran for 52550 frames Cell Events: 4264 mean_circ: 0.940243
Ran for 56550 frames Cell Events: 4576 mean_circ: 0.940313
Ran for 58550 frames Cell Events: 4712 mean_circ: 0.94028
Ran for 59050 frames Cell Events: 4758 mean_circ: 0.94031
Ran for 62050 frames Cell Events: 5004 mean_circ: 0.940307
Ran for 69050 frames Cell Events: 5567 mean_circ: 0.940317
Ran for 70050 frames Cell Events: 5650 mean_circ: 0.940317
Ran for 76550 frames Cell Events: 6195 mean_circ: 0.940228
Ran for 84050 frames Cell Events: 6774 mean_circ: 0.939877
Ran for 86050 frames Cell Events: 6957 mean_circ: 0.939877
Ran for 89550 frames Cell Events: 7291 mean_circ: 0.940069
Ran for 91550 frames Cell Events: 7452 mean_circ: 0.939984
Ran for 96050 frames Cell Events: 7821 mean_circ: 0.939991
Ran for 99050 frames Cell Events: 8037 mean_circ: 0.940087
End this measurment..
Stopping time: 1.67274e+009
****************************************
Measurement finished
Mean Circularitiy: 0.940049
Mean Area: 616.585
Mean FL-1 intensity: 3451.98
Mean FL-2 intensity: 3482.6
Mean FL-3 intensity: 0
frames processed: 94058
Total Time: 49748.4ms
Number of Runs (new bg img) 0
Frames collected: 99400
Skipped frames: 80082
Rescued Frames: 2242993
Samples DAQ: 24850457
Samples written: 49699736
Samples per Channel: 24849868
FPS: 1998.05
Samples per second: 499511
Expected samples/s: 500000
Events: 8064
Events per second: 162.096
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :123917
# 1 frames :17167
# 2 frames :1196
# 3 frames :550
# 4 frames :302
# 5 frames :250
# 6 frames :970
# 7 frames :587
# 8 frames :488
# 9 frames :395
# 10 frames :379
# 11 frames :243
# 12 frames :164
# 13 frames :146
# 14 frames :167
# 15 frames :174
# 16 frames :150
# 17 frames :122
# 18 frames :119
# 19 frames :77
# 20 frames :75
# 21 frames :83
# 22 frames :77
# 23 frames :59
# 24 frames :64
# 25 frames :46
# 26 frames :52
# 27 frames :46
# 28 frames :56
# 29 frames :34
# 30 frames :44
# 31 frames :37
# 32 frames :38
# 33 frames :35
# 34 frames :26
# 35 frames :17
# 36 frames :30
# 37 frames :24
# 38 frames :22
# 39 frames :8
# 40 frames :15
# 41 frames :18
# 42 frames :13
# 43 frames :13
# 44 frames :12
# 45 frames :14
# 46 frames :11
# 47 frames :14
# 48 frames :11
# 49 frames :7
# 50 frames :9
# 51 frames :4
# 52 frames :4
# 53 frames :9
# 54 frames :7
# 55 frames :2
# 56 frames :6
# 57 frames :4
# 58 frames :2
# 59 frames :3
# 60 frames :2
# 61 frames :5
# 62 frames :2
# 63 frames :4
# 64 frames :1
# 65 frames :2
# 66 frames :3
# 67 frames :1
# 68 frames :5
# 69 frames :1
# 71 frames :1
# 72 frames :2
# 73 frames :1
# 75 frames :1
# 77 frames :1
# 78 frames :1
# 82 frames :1
# 86 frames :1
# 93 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4325
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0