Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.16.21
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "38631e7f6ecf109d3ce90e01eec56fec",
"name": "M1_20170603_1734_dsRNA_M4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:16:43"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8812 (max 8813) in '8812'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4285
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67467e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 36 mean_circ: 0.954539
Ran for 1550 frames Cell Events: 51 mean_circ: 0.954281
Ran for 2050 frames Cell Events: 68 mean_circ: 0.95357
Ran for 2550 frames Cell Events: 88 mean_circ: 0.95476
Ran for 3050 frames Cell Events: 110 mean_circ: 0.954027
Ran for 3550 frames Cell Events: 133 mean_circ: 0.952482
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Ran for 6050 frames Cell Events: 236 mean_circ: 0.954141
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Ran for 9050 frames Cell Events: 369 mean_circ: 0.949963
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Ran for 84050 frames Cell Events: 2869 mean_circ: 0.947828
Ran for 84550 frames Cell Events: 2884 mean_circ: 0.947842
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Ran for 86050 frames Cell Events: 2939 mean_circ: 0.947684
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Ran for 92050 frames Cell Events: 3138 mean_circ: 0.947745
Ran for 94050 frames Cell Events: 3204 mean_circ: 0.947771
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Ran for 96050 frames Cell Events: 3270 mean_circ: 0.947769
Ran for 96550 frames Cell Events: 3285 mean_circ: 0.947759
Ran for 97550 frames Cell Events: 3326 mean_circ: 0.947772
Ran for 99050 frames Cell Events: 3386 mean_circ: 0.94785
Ran for 100050 frames Cell Events: 3418 mean_circ: 0.947951
Ran for 100550 frames Cell Events: 3436 mean_circ: 0.947947
Ran for 101550 frames Cell Events: 3474 mean_circ: 0.947878
Ran for 102050 frames Cell Events: 3488 mean_circ: 0.947893
Ran for 103550 frames Cell Events: 3543 mean_circ: 0.947859
Ran for 106550 frames Cell Events: 3654 mean_circ: 0.947808
Ran for 107050 frames Cell Events: 3666 mean_circ: 0.947787
Ran for 107550 frames Cell Events: 3681 mean_circ: 0.947807
Ran for 109050 frames Cell Events: 3739 mean_circ: 0.947832
Ran for 109550 frames Cell Events: 3759 mean_circ: 0.947849
Ran for 110050 frames Cell Events: 3771 mean_circ: 0.947851
Ran for 110550 frames Cell Events: 3802 mean_circ: 0.947843
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Ran for 112050 frames Cell Events: 3839 mean_circ: 0.947839
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Ran for 126550 frames Cell Events: 4407 mean_circ: 0.947902
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Ran for 132050 frames Cell Events: 4627 mean_circ: 0.947981
Ran for 133050 frames Cell Events: 4674 mean_circ: 0.947966
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Ran for 143550 frames Cell Events: 5062 mean_circ: 0.947957
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Ran for 147050 frames Cell Events: 5212 mean_circ: 0.947761
Ran for 148050 frames Cell Events: 5252 mean_circ: 0.947753
Ran for 149550 frames Cell Events: 5307 mean_circ: 0.947712
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Ran for 151050 frames Cell Events: 5366 mean_circ: 0.947721
Ran for 152050 frames Cell Events: 5395 mean_circ: 0.9477
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Ran for 153050 frames Cell Events: 5433 mean_circ: 0.947726
Ran for 154550 frames Cell Events: 5508 mean_circ: 0.947737
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Ran for 156550 frames Cell Events: 5594 mean_circ: 0.947637
Ran for 157050 frames Cell Events: 5619 mean_circ: 0.947645
Ran for 158050 frames Cell Events: 5665 mean_circ: 0.947704
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Ran for 159050 frames Cell Events: 5692 mean_circ: 0.947719
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Ran for 161550 frames Cell Events: 5793 mean_circ: 0.947728
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Ran for 163550 frames Cell Events: 5876 mean_circ: 0.947717
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Ran for 225550 frames Cell Events: 8250 mean_circ: 0.947189
Ran for 226050 frames Cell Events: 8268 mean_circ: 0.947195
Ran for 227050 frames Cell Events: 8310 mean_circ: 0.947218
Ran for 228050 frames Cell Events: 8350 mean_circ: 0.947234
Ran for 228550 frames Cell Events: 8366 mean_circ: 0.947243
Ran for 229550 frames Cell Events: 8403 mean_circ: 0.947217
Ran for 230050 frames Cell Events: 8411 mean_circ: 0.947221
Ran for 230550 frames Cell Events: 8430 mean_circ: 0.947201
Ran for 231550 frames Cell Events: 8465 mean_circ: 0.947203
Ran for 232050 frames Cell Events: 8483 mean_circ: 0.947208
Ran for 233050 frames Cell Events: 8521 mean_circ: 0.947198
Ran for 233550 frames Cell Events: 8532 mean_circ: 0.947217
Ran for 234050 frames Cell Events: 8554 mean_circ: 0.947225
Ran for 235050 frames Cell Events: 8582 mean_circ: 0.947266
Ran for 236050 frames Cell Events: 8624 mean_circ: 0.947291
Ran for 238050 frames Cell Events: 8690 mean_circ: 0.94729
Ran for 238550 frames Cell Events: 8711 mean_circ: 0.947306
Ran for 241050 frames Cell Events: 8812 mean_circ: 0.947218
End this measurment..
Stopping time: 1.67479e+009
****************************************
Measurement finished
Mean Circularitiy: 0.947114
Mean Area: 626.945
Mean FL-1 intensity: 3608.9
Mean FL-2 intensity: 3348.62
Mean FL-3 intensity: 0
frames processed: 237324
Total Time: 120626ms
Number of Runs (new bg img) 0
Frames collected: 241099
Skipped frames: 99997
Rescued Frames: 2715702
Samples DAQ: 60274695
Samples written: 120549226
Samples per Channel: 60274613
FPS: 1998.73
Samples per second: 499682
Expected samples/s: 500000
Events: 8814
Events per second: 73.0689
jumped back (PMT): 4157
#Frames read, frequency
# 0 frames :360767
# 1 frames :103368
# 2 frames :4383
# 3 frames :1963
# 4 frames :1177
# 5 frames :672
# 6 frames :1139
# 7 frames :1633
# 8 frames :1466
# 9 frames :1036
# 10 frames :693
# 11 frames :604
# 12 frames :510
# 13 frames :370
# 14 frames :348
# 15 frames :312
# 16 frames :253
# 17 frames :207
# 18 frames :198
# 19 frames :114
# 20 frames :116
# 21 frames :105
# 22 frames :86
# 23 frames :85
# 24 frames :66
# 25 frames :61
# 26 frames :52
# 27 frames :38
# 28 frames :34
# 29 frames :40
# 30 frames :22
# 31 frames :19
# 32 frames :26
# 33 frames :16
# 34 frames :18
# 35 frames :18
# 36 frames :16
# 37 frames :12
# 38 frames :9
# 39 frames :11
# 40 frames :14
# 41 frames :12
# 42 frames :6
# 43 frames :7
# 44 frames :6
# 45 frames :6
# 46 frames :5
# 47 frames :4
# 48 frames :2
# 49 frames :3
# 50 frames :3
# 51 frames :4
# 52 frames :2
# 53 frames :1
# 55 frames :2
# 56 frames :3
# 57 frames :3
# 59 frames :1
# 60 frames :2
# 61 frames :1
# 63 frames :1
# 70 frames :1
# 72 frames :1
# 76 frames :1
# 81 frames :1
# 86 frames :1
# 91 frames :1
# 93 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4285
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0