Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.22.08
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a967159d4accf36ed95edabf7260de5f",
"name": "M1_20170603_1851_dsRNA_C2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:22:28"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8013 (max 8014) in '8013'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9467
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67929e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 3050 frames Cell Events: 121 mean_circ: 0.933377
Ran for 3550 frames Cell Events: 138 mean_circ: 0.934395
Ran for 5050 frames Cell Events: 201 mean_circ: 0.936237
Ran for 5550 frames Cell Events: 215 mean_circ: 0.935454
Ran for 6050 frames Cell Events: 231 mean_circ: 0.935602
Ran for 6550 frames Cell Events: 252 mean_circ: 0.935427
Ran for 7050 frames Cell Events: 270 mean_circ: 0.935399
Ran for 7550 frames Cell Events: 280 mean_circ: 0.934957
Ran for 8550 frames Cell Events: 324 mean_circ: 0.935015
Ran for 9050 frames Cell Events: 342 mean_circ: 0.935209
Ran for 9550 frames Cell Events: 360 mean_circ: 0.934638
Ran for 10050 frames Cell Events: 380 mean_circ: 0.934825
Ran for 10550 frames Cell Events: 399 mean_circ: 0.934582
Ran for 11050 frames Cell Events: 418 mean_circ: 0.934833
Ran for 12050 frames Cell Events: 466 mean_circ: 0.934245
Ran for 12550 frames Cell Events: 481 mean_circ: 0.934054
Ran for 13050 frames Cell Events: 503 mean_circ: 0.934075
Ran for 14050 frames Cell Events: 543 mean_circ: 0.934492
Ran for 14550 frames Cell Events: 573 mean_circ: 0.934349
Ran for 15050 frames Cell Events: 590 mean_circ: 0.934528
Ran for 15550 frames Cell Events: 618 mean_circ: 0.935078
Ran for 16050 frames Cell Events: 642 mean_circ: 0.935652
Ran for 16550 frames Cell Events: 671 mean_circ: 0.935976
Ran for 17050 frames Cell Events: 686 mean_circ: 0.936272
Ran for 17550 frames Cell Events: 708 mean_circ: 0.935839
Ran for 18050 frames Cell Events: 731 mean_circ: 0.936367
Ran for 18550 frames Cell Events: 754 mean_circ: 0.93595
Ran for 19050 frames Cell Events: 775 mean_circ: 0.935956
Ran for 19550 frames Cell Events: 803 mean_circ: 0.936237
Ran for 20050 frames Cell Events: 824 mean_circ: 0.936553
Ran for 21550 frames Cell Events: 876 mean_circ: 0.93653
Ran for 22050 frames Cell Events: 890 mean_circ: 0.936447
Ran for 22550 frames Cell Events: 912 mean_circ: 0.936399
Ran for 23550 frames Cell Events: 963 mean_circ: 0.936497
Ran for 24050 frames Cell Events: 992 mean_circ: 0.936221
Ran for 24550 frames Cell Events: 1009 mean_circ: 0.936395
Ran for 25050 frames Cell Events: 1029 mean_circ: 0.936325
Ran for 26050 frames Cell Events: 1069 mean_circ: 0.936401
Ran for 27550 frames Cell Events: 1142 mean_circ: 0.936582
Ran for 28050 frames Cell Events: 1166 mean_circ: 0.936656
Ran for 28550 frames Cell Events: 1187 mean_circ: 0.936698
Ran for 30550 frames Cell Events: 1306 mean_circ: 0.936231
Ran for 31050 frames Cell Events: 1329 mean_circ: 0.936389
Ran for 31550 frames Cell Events: 1340 mean_circ: 0.936361
Ran for 32550 frames Cell Events: 1395 mean_circ: 0.936201
Ran for 33550 frames Cell Events: 1435 mean_circ: 0.936296
Ran for 34050 frames Cell Events: 1448 mean_circ: 0.936132
Ran for 35550 frames Cell Events: 1522 mean_circ: 0.93579
Ran for 36050 frames Cell Events: 1540 mean_circ: 0.935834
Ran for 36550 frames Cell Events: 1562 mean_circ: 0.935898
Ran for 37050 frames Cell Events: 1578 mean_circ: 0.935911
Ran for 39050 frames Cell Events: 1656 mean_circ: 0.936002
Ran for 41050 frames Cell Events: 1741 mean_circ: 0.936023
Ran for 41550 frames Cell Events: 1765 mean_circ: 0.936058
Ran for 42050 frames Cell Events: 1788 mean_circ: 0.936084
Ran for 43550 frames Cell Events: 1863 mean_circ: 0.936074
Ran for 45050 frames Cell Events: 1954 mean_circ: 0.936009
Ran for 45550 frames Cell Events: 1978 mean_circ: 0.935974
Ran for 46550 frames Cell Events: 2031 mean_circ: 0.935812
Ran for 47050 frames Cell Events: 2057 mean_circ: 0.935756
Ran for 48550 frames Cell Events: 2119 mean_circ: 0.935808
Ran for 49050 frames Cell Events: 2144 mean_circ: 0.935774
Ran for 49550 frames Cell Events: 2172 mean_circ: 0.935813
Ran for 52050 frames Cell Events: 2297 mean_circ: 0.935947
Ran for 54050 frames Cell Events: 2380 mean_circ: 0.935861
Ran for 54550 frames Cell Events: 2403 mean_circ: 0.935879
Ran for 55050 frames Cell Events: 2426 mean_circ: 0.935922
Ran for 56050 frames Cell Events: 2487 mean_circ: 0.935905
Ran for 56550 frames Cell Events: 2503 mean_circ: 0.935946
Ran for 57550 frames Cell Events: 2553 mean_circ: 0.93592
Ran for 58550 frames Cell Events: 2594 mean_circ: 0.935981
Ran for 59550 frames Cell Events: 2628 mean_circ: 0.93593
Ran for 60550 frames Cell Events: 2667 mean_circ: 0.935909
Ran for 61050 frames Cell Events: 2684 mean_circ: 0.935938
Ran for 61550 frames Cell Events: 2703 mean_circ: 0.935973
Ran for 62050 frames Cell Events: 2727 mean_circ: 0.935965
Ran for 62550 frames Cell Events: 2758 mean_circ: 0.935994
Ran for 63050 frames Cell Events: 2784 mean_circ: 0.935904
Ran for 64550 frames Cell Events: 2857 mean_circ: 0.936038
Ran for 65050 frames Cell Events: 2875 mean_circ: 0.936074
Ran for 65550 frames Cell Events: 2900 mean_circ: 0.936077
Ran for 66050 frames Cell Events: 2912 mean_circ: 0.936021
Ran for 67050 frames Cell Events: 2940 mean_circ: 0.936004
Ran for 68550 frames Cell Events: 2999 mean_circ: 0.936185
Ran for 69550 frames Cell Events: 3030 mean_circ: 0.936242
Ran for 70050 frames Cell Events: 3048 mean_circ: 0.936185
Ran for 70550 frames Cell Events: 3065 mean_circ: 0.936214
Ran for 71050 frames Cell Events: 3081 mean_circ: 0.936147
Ran for 72550 frames Cell Events: 3163 mean_circ: 0.936026
Ran for 73050 frames Cell Events: 3179 mean_circ: 0.936051
Ran for 74050 frames Cell Events: 3218 mean_circ: 0.936083
Ran for 75050 frames Cell Events: 3257 mean_circ: 0.936003
Ran for 76050 frames Cell Events: 3294 mean_circ: 0.936049
Ran for 76550 frames Cell Events: 3312 mean_circ: 0.935996
Ran for 77050 frames Cell Events: 3336 mean_circ: 0.935912
Ran for 78050 frames Cell Events: 3389 mean_circ: 0.935922
Ran for 78550 frames Cell Events: 3419 mean_circ: 0.935817
Ran for 79050 frames Cell Events: 3436 mean_circ: 0.935789
Ran for 79550 frames Cell Events: 3456 mean_circ: 0.935812
Ran for 80050 frames Cell Events: 3478 mean_circ: 0.935906
Ran for 80550 frames Cell Events: 3500 mean_circ: 0.935893
Ran for 81050 frames Cell Events: 3521 mean_circ: 0.935943
Ran for 82550 frames Cell Events: 3571 mean_circ: 0.93603
Ran for 86050 frames Cell Events: 3771 mean_circ: 0.936137
Ran for 87050 frames Cell Events: 3816 mean_circ: 0.936101
Ran for 88550 frames Cell Events: 3897 mean_circ: 0.936099
Ran for 89550 frames Cell Events: 3938 mean_circ: 0.936061
Ran for 90550 frames Cell Events: 3969 mean_circ: 0.936015
Ran for 91550 frames Cell Events: 4011 mean_circ: 0.936036
Ran for 92050 frames Cell Events: 4039 mean_circ: 0.935885
Ran for 93550 frames Cell Events: 4112 mean_circ: 0.935856
Ran for 94050 frames Cell Events: 4133 mean_circ: 0.93588
Ran for 94550 frames Cell Events: 4155 mean_circ: 0.93584
Ran for 95050 frames Cell Events: 4172 mean_circ: 0.935816
Ran for 96050 frames Cell Events: 4218 mean_circ: 0.935777
Ran for 97050 frames Cell Events: 4271 mean_circ: 0.935741
Ran for 98050 frames Cell Events: 4322 mean_circ: 0.935684
Ran for 101050 frames Cell Events: 4454 mean_circ: 0.935784
Ran for 102050 frames Cell Events: 4494 mean_circ: 0.935769
Ran for 103550 frames Cell Events: 4555 mean_circ: 0.935868
Ran for 104550 frames Cell Events: 4611 mean_circ: 0.935898
Ran for 105050 frames Cell Events: 4634 mean_circ: 0.935883
Ran for 105550 frames Cell Events: 4658 mean_circ: 0.93582
Ran for 106550 frames Cell Events: 4703 mean_circ: 0.935751
Ran for 107050 frames Cell Events: 4723 mean_circ: 0.935768
Ran for 110050 frames Cell Events: 4840 mean_circ: 0.935812
Ran for 111050 frames Cell Events: 4886 mean_circ: 0.935819
Ran for 112550 frames Cell Events: 4955 mean_circ: 0.935916
Ran for 113050 frames Cell Events: 4977 mean_circ: 0.935878
Ran for 115050 frames Cell Events: 5063 mean_circ: 0.935791
Ran for 116050 frames Cell Events: 5111 mean_circ: 0.935771
Ran for 117050 frames Cell Events: 5157 mean_circ: 0.935802
Ran for 117550 frames Cell Events: 5168 mean_circ: 0.935828
Ran for 118550 frames Cell Events: 5206 mean_circ: 0.935861
Ran for 119050 frames Cell Events: 5229 mean_circ: 0.935877
Ran for 119550 frames Cell Events: 5251 mean_circ: 0.935915
Ran for 120050 frames Cell Events: 5262 mean_circ: 0.935869
Ran for 120550 frames Cell Events: 5284 mean_circ: 0.935863
Ran for 121050 frames Cell Events: 5303 mean_circ: 0.935823
Ran for 122050 frames Cell Events: 5355 mean_circ: 0.935709
Ran for 123050 frames Cell Events: 5400 mean_circ: 0.935667
Ran for 123550 frames Cell Events: 5422 mean_circ: 0.935645
Ran for 124550 frames Cell Events: 5458 mean_circ: 0.935658
Ran for 125050 frames Cell Events: 5476 mean_circ: 0.935686
Ran for 125550 frames Cell Events: 5494 mean_circ: 0.935609
Ran for 126550 frames Cell Events: 5542 mean_circ: 0.935626
Ran for 127550 frames Cell Events: 5598 mean_circ: 0.935563
Ran for 128050 frames Cell Events: 5620 mean_circ: 0.935495
Ran for 129050 frames Cell Events: 5652 mean_circ: 0.935476
Ran for 129550 frames Cell Events: 5674 mean_circ: 0.9354
Ran for 131050 frames Cell Events: 5740 mean_circ: 0.935389
Ran for 132050 frames Cell Events: 5799 mean_circ: 0.935315
Ran for 132550 frames Cell Events: 5819 mean_circ: 0.935282
Ran for 133050 frames Cell Events: 5838 mean_circ: 0.935326
Ran for 133550 frames Cell Events: 5869 mean_circ: 0.935253
Ran for 134550 frames Cell Events: 5905 mean_circ: 0.935279
Ran for 135050 frames Cell Events: 5919 mean_circ: 0.935288
Ran for 135550 frames Cell Events: 5940 mean_circ: 0.93525
Ran for 136550 frames Cell Events: 5981 mean_circ: 0.935293
Ran for 137550 frames Cell Events: 6007 mean_circ: 0.935262
Ran for 138050 frames Cell Events: 6020 mean_circ: 0.935231
Ran for 138550 frames Cell Events: 6040 mean_circ: 0.935215
Ran for 139050 frames Cell Events: 6061 mean_circ: 0.935275
Ran for 140550 frames Cell Events: 6149 mean_circ: 0.935225
Ran for 141050 frames Cell Events: 6171 mean_circ: 0.935177
Ran for 141550 frames Cell Events: 6186 mean_circ: 0.935144
Ran for 142550 frames Cell Events: 6246 mean_circ: 0.935101
Ran for 143050 frames Cell Events: 6275 mean_circ: 0.935109
Ran for 144050 frames Cell Events: 6334 mean_circ: 0.935084
Ran for 144550 frames Cell Events: 6353 mean_circ: 0.935074
Ran for 145050 frames Cell Events: 6384 mean_circ: 0.935069
Ran for 145550 frames Cell Events: 6406 mean_circ: 0.935023
Ran for 146550 frames Cell Events: 6451 mean_circ: 0.935098
Ran for 149050 frames Cell Events: 6568 mean_circ: 0.935086
Ran for 150550 frames Cell Events: 6640 mean_circ: 0.93507
Ran for 152550 frames Cell Events: 6728 mean_circ: 0.935124
Ran for 153050 frames Cell Events: 6746 mean_circ: 0.93511
Ran for 153550 frames Cell Events: 6775 mean_circ: 0.935109
Ran for 154050 frames Cell Events: 6797 mean_circ: 0.935083
Ran for 154550 frames Cell Events: 6816 mean_circ: 0.935078
Ran for 155050 frames Cell Events: 6839 mean_circ: 0.935095
Ran for 155550 frames Cell Events: 6879 mean_circ: 0.935066
Ran for 156050 frames Cell Events: 6907 mean_circ: 0.935055
Ran for 156550 frames Cell Events: 6929 mean_circ: 0.93504
Ran for 157050 frames Cell Events: 6951 mean_circ: 0.935028
Ran for 157550 frames Cell Events: 6966 mean_circ: 0.93502
Ran for 158050 frames Cell Events: 6989 mean_circ: 0.935029
Ran for 158550 frames Cell Events: 7010 mean_circ: 0.935023
Ran for 160550 frames Cell Events: 7099 mean_circ: 0.935029
Ran for 161050 frames Cell Events: 7113 mean_circ: 0.935043
Ran for 161550 frames Cell Events: 7138 mean_circ: 0.935051
Ran for 162550 frames Cell Events: 7174 mean_circ: 0.935046
Ran for 163050 frames Cell Events: 7205 mean_circ: 0.935038
Ran for 163550 frames Cell Events: 7226 mean_circ: 0.935048
Ran for 164550 frames Cell Events: 7272 mean_circ: 0.935017
Ran for 168050 frames Cell Events: 7435 mean_circ: 0.935013
Ran for 168550 frames Cell Events: 7456 mean_circ: 0.935012
Ran for 169050 frames Cell Events: 7479 mean_circ: 0.935023
Ran for 170050 frames Cell Events: 7521 mean_circ: 0.934966
Ran for 171050 frames Cell Events: 7575 mean_circ: 0.934943
Ran for 172550 frames Cell Events: 7643 mean_circ: 0.934878
Ran for 173050 frames Cell Events: 7667 mean_circ: 0.934868
Ran for 174550 frames Cell Events: 7736 mean_circ: 0.934838
Ran for 177550 frames Cell Events: 7883 mean_circ: 0.934842
Ran for 178050 frames Cell Events: 7898 mean_circ: 0.934855
Ran for 179550 frames Cell Events: 7957 mean_circ: 0.934875
Ran for 180050 frames Cell Events: 7984 mean_circ: 0.934909
End this measurment..
Stopping time: 1.67938e+009
****************************************
Measurement finished
Mean Circularitiy: 0.934852
Mean Area: 641.999
Mean FL-1 intensity: 3315.01
Mean FL-2 intensity: 3925.66
Mean FL-3 intensity: 0
frames processed: 179539
Total Time: 90556ms
Number of Runs (new bg img) 0
Frames collected: 180988
Skipped frames: 145940
Rescued Frames: 3953583
Samples DAQ: 45247078
Samples written: 90494034
Samples per Channel: 45247017
FPS: 1998.63
Samples per second: 499658
Expected samples/s: 500000
Events: 8014
Events per second: 88.4977
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :266398
# 1 frames :81035
# 2 frames :3830
# 3 frames :1404
# 4 frames :729
# 5 frames :323
# 6 frames :1764
# 7 frames :1437
# 8 frames :1062
# 9 frames :655
# 10 frames :927
# 11 frames :468
# 12 frames :406
# 13 frames :213
# 14 frames :223
# 15 frames :161
# 16 frames :170
# 17 frames :130
# 18 frames :92
# 19 frames :65
# 20 frames :65
# 21 frames :55
# 22 frames :50
# 23 frames :39
# 24 frames :28
# 25 frames :30
# 26 frames :27
# 27 frames :20
# 28 frames :20
# 29 frames :20
# 30 frames :12
# 31 frames :10
# 32 frames :6
# 33 frames :4
# 34 frames :9
# 35 frames :7
# 36 frames :5
# 37 frames :10
# 38 frames :6
# 40 frames :3
# 41 frames :1
# 42 frames :2
# 43 frames :4
# 44 frames :4
# 46 frames :2
# 47 frames :3
# 48 frames :2
# 49 frames :1
# 50 frames :2
# 52 frames :1
# 55 frames :1
# 58 frames :1
# 59 frames :1
# 72 frames :1
# 92 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9467
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0