Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.22.35
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "49048b2f1cdb937bda8ae208bf2fcc22",
"name": "M1_20170603_1859_dsRNA_B4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:22:56"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8603 (max 8604) in '8603'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9958
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67981e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 32 mean_circ: 0.946756
Ran for 1550 frames Cell Events: 51 mean_circ: 0.948434
Ran for 2050 frames Cell Events: 70 mean_circ: 0.944864
Ran for 3050 frames Cell Events: 110 mean_circ: 0.948459
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Ran for 8050 frames Cell Events: 279 mean_circ: 0.947481
Ran for 10050 frames Cell Events: 363 mean_circ: 0.947436
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Ran for 73550 frames Cell Events: 2703 mean_circ: 0.944345
Ran for 74050 frames Cell Events: 2717 mean_circ: 0.94417
Ran for 77550 frames Cell Events: 2847 mean_circ: 0.944202
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Ran for 81050 frames Cell Events: 2976 mean_circ: 0.944122
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Ran for 91050 frames Cell Events: 3384 mean_circ: 0.943956
Ran for 93050 frames Cell Events: 3459 mean_circ: 0.943951
Ran for 95050 frames Cell Events: 3546 mean_circ: 0.943966
Ran for 95550 frames Cell Events: 3563 mean_circ: 0.944009
Ran for 96050 frames Cell Events: 3587 mean_circ: 0.943966
Ran for 97050 frames Cell Events: 3623 mean_circ: 0.943919
Ran for 98050 frames Cell Events: 3663 mean_circ: 0.943933
Ran for 99050 frames Cell Events: 3710 mean_circ: 0.943879
Ran for 99550 frames Cell Events: 3726 mean_circ: 0.943855
Ran for 100550 frames Cell Events: 3784 mean_circ: 0.943972
Ran for 101050 frames Cell Events: 3796 mean_circ: 0.944025
Ran for 101550 frames Cell Events: 3816 mean_circ: 0.944
Ran for 102050 frames Cell Events: 3827 mean_circ: 0.944045
Ran for 104550 frames Cell Events: 3921 mean_circ: 0.943989
Ran for 105550 frames Cell Events: 3960 mean_circ: 0.944026
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Ran for 106550 frames Cell Events: 3996 mean_circ: 0.944097
Ran for 107050 frames Cell Events: 4009 mean_circ: 0.944102
Ran for 107550 frames Cell Events: 4030 mean_circ: 0.944176
Ran for 108050 frames Cell Events: 4043 mean_circ: 0.944174
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Ran for 121550 frames Cell Events: 4544 mean_circ: 0.944453
Ran for 122050 frames Cell Events: 4562 mean_circ: 0.944438
Ran for 122550 frames Cell Events: 4579 mean_circ: 0.944457
Ran for 123050 frames Cell Events: 4598 mean_circ: 0.944466
Ran for 123550 frames Cell Events: 4611 mean_circ: 0.94448
Ran for 124050 frames Cell Events: 4630 mean_circ: 0.944488
Ran for 124550 frames Cell Events: 4646 mean_circ: 0.944516
Ran for 125050 frames Cell Events: 4667 mean_circ: 0.94454
Ran for 126050 frames Cell Events: 4700 mean_circ: 0.944578
Ran for 126550 frames Cell Events: 4710 mean_circ: 0.944554
Ran for 127050 frames Cell Events: 4725 mean_circ: 0.94453
Ran for 127550 frames Cell Events: 4744 mean_circ: 0.944459
Ran for 128050 frames Cell Events: 4768 mean_circ: 0.944481
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Ran for 131050 frames Cell Events: 4897 mean_circ: 0.944429
Ran for 133050 frames Cell Events: 4985 mean_circ: 0.944437
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Ran for 143050 frames Cell Events: 5360 mean_circ: 0.944057
Ran for 144050 frames Cell Events: 5413 mean_circ: 0.944062
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Ran for 146550 frames Cell Events: 5498 mean_circ: 0.94408
Ran for 147550 frames Cell Events: 5532 mean_circ: 0.944108
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Ran for 152050 frames Cell Events: 5706 mean_circ: 0.944085
Ran for 152550 frames Cell Events: 5727 mean_circ: 0.944147
Ran for 153050 frames Cell Events: 5753 mean_circ: 0.944136
Ran for 153550 frames Cell Events: 5777 mean_circ: 0.944145
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Ran for 182550 frames Cell Events: 6949 mean_circ: 0.943807
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Ran for 196050 frames Cell Events: 7463 mean_circ: 0.943726
Ran for 197050 frames Cell Events: 7511 mean_circ: 0.943708
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Ran for 201550 frames Cell Events: 7663 mean_circ: 0.943731
Ran for 202050 frames Cell Events: 7690 mean_circ: 0.943703
Ran for 202550 frames Cell Events: 7711 mean_circ: 0.943712
Ran for 203050 frames Cell Events: 7731 mean_circ: 0.943723
Ran for 203550 frames Cell Events: 7741 mean_circ: 0.943706
Ran for 204550 frames Cell Events: 7795 mean_circ: 0.943671
Ran for 205050 frames Cell Events: 7818 mean_circ: 0.943636
Ran for 205550 frames Cell Events: 7833 mean_circ: 0.943656
Ran for 206050 frames Cell Events: 7846 mean_circ: 0.943669
Ran for 206550 frames Cell Events: 7854 mean_circ: 0.943662
Ran for 208550 frames Cell Events: 7932 mean_circ: 0.943586
Ran for 209050 frames Cell Events: 7949 mean_circ: 0.943588
Ran for 209550 frames Cell Events: 7964 mean_circ: 0.943591
Ran for 210050 frames Cell Events: 7972 mean_circ: 0.943599
Ran for 210550 frames Cell Events: 7993 mean_circ: 0.943606
Ran for 211050 frames Cell Events: 8012 mean_circ: 0.943608
Ran for 211550 frames Cell Events: 8033 mean_circ: 0.94358
Ran for 212050 frames Cell Events: 8055 mean_circ: 0.943565
Ran for 212550 frames Cell Events: 8076 mean_circ: 0.943521
Ran for 213050 frames Cell Events: 8099 mean_circ: 0.943529
Ran for 213550 frames Cell Events: 8115 mean_circ: 0.943549
Ran for 214050 frames Cell Events: 8136 mean_circ: 0.943566
Ran for 214550 frames Cell Events: 8157 mean_circ: 0.943567
Ran for 215050 frames Cell Events: 8180 mean_circ: 0.943539
Ran for 216050 frames Cell Events: 8218 mean_circ: 0.943538
Ran for 216550 frames Cell Events: 8238 mean_circ: 0.943564
Ran for 217550 frames Cell Events: 8271 mean_circ: 0.943567
Ran for 218050 frames Cell Events: 8287 mean_circ: 0.943601
Ran for 218550 frames Cell Events: 8320 mean_circ: 0.943586
Ran for 219550 frames Cell Events: 8378 mean_circ: 0.943581
Ran for 220050 frames Cell Events: 8391 mean_circ: 0.943521
Ran for 221550 frames Cell Events: 8444 mean_circ: 0.943516
Ran for 222050 frames Cell Events: 8468 mean_circ: 0.943506
Ran for 223050 frames Cell Events: 8490 mean_circ: 0.943521
Ran for 223550 frames Cell Events: 8511 mean_circ: 0.943489
Ran for 224050 frames Cell Events: 8530 mean_circ: 0.943507
Ran for 225550 frames Cell Events: 8598 mean_circ: 0.943507
End this measurment..
Stopping time: 1.67992e+009
****************************************
Measurement finished
Mean Circularitiy: 0.943509
Mean Area: 641.949
Mean FL-1 intensity: 3251.21
Mean FL-2 intensity: 3572.11
Mean FL-3 intensity: 0
frames processed: 224192
Total Time: 112884ms
Number of Runs (new bg img) 0
Frames collected: 225632
Skipped frames: 147380
Rescued Frames: 4048356
Samples DAQ: 56408013
Samples written: 112815944
Samples per Channel: 56407972
FPS: 1998.8
Samples per second: 499699
Expected samples/s: 500000
Events: 8604
Events per second: 76.2199
jumped back (PMT): 4928
#Frames read, frequency
# 0 frames :321753
# 1 frames :112036
# 2 frames :5040
# 3 frames :1759
# 4 frames :924
# 5 frames :390
# 6 frames :1819
# 7 frames :1835
# 8 frames :1170
# 9 frames :724
# 10 frames :1051
# 11 frames :518
# 12 frames :544
# 13 frames :254
# 14 frames :217
# 15 frames :183
# 16 frames :201
# 17 frames :148
# 18 frames :115
# 19 frames :86
# 20 frames :75
# 21 frames :53
# 22 frames :43
# 23 frames :32
# 24 frames :32
# 25 frames :27
# 26 frames :18
# 27 frames :20
# 28 frames :17
# 29 frames :8
# 30 frames :10
# 31 frames :12
# 32 frames :7
# 33 frames :6
# 34 frames :10
# 35 frames :7
# 36 frames :3
# 37 frames :4
# 38 frames :5
# 39 frames :3
# 40 frames :1
# 41 frames :2
# 42 frames :4
# 44 frames :2
# 45 frames :2
# 48 frames :1
# 49 frames :1
# 52 frames :1
# 55 frames :1
# 58 frames :1
# 63 frames :1
# 69 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9958
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0