Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.27.47
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "30f87f3673a9a39bd128aca71a6117cb",
"name": "M1_20170603_2003_dsRNA_D6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:28:07"
}
}
dclab-tdms2rtdc-warnings
MultipleSamplesPerEventFound
in dclab.rtdc_dataset.fmt_tdms.exc line 101:
Ignoring trace data of
'\\syn03.frontend.storage.int.mpl.mpg.de\guck_division2\Members\Laura\20240219_Kasia_screen_all_folders\20170603_katarzyna_kc167_phospho_screen\20170603_2003_dsRNA_D6_faster\M1_20170603_2003_dsRNA_D6_faster_traces.tdms'
due to multiple values for samples per event: [200 400]
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8128 (max 8129) in '8128'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9768
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.68363e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2550 frames Cell Events: 112 mean_circ: 0.942429
Ran for 3050 frames Cell Events: 129 mean_circ: 0.942126
Ran for 4050 frames Cell Events: 171 mean_circ: 0.942384
Ran for 4550 frames Cell Events: 187 mean_circ: 0.942577
Ran for 5050 frames Cell Events: 209 mean_circ: 0.942426
Ran for 6050 frames Cell Events: 257 mean_circ: 0.940126
Ran for 8050 frames Cell Events: 305 mean_circ: 0.941163
Ran for 9550 frames Cell Events: 366 mean_circ: 0.941286
Ran for 10050 frames Cell Events: 381 mean_circ: 0.941385
Ran for 10550 frames Cell Events: 392 mean_circ: 0.940805
Ran for 11050 frames Cell Events: 402 mean_circ: 0.941063
Ran for 11550 frames Cell Events: 425 mean_circ: 0.94115
Ran for 12050 frames Cell Events: 436 mean_circ: 0.941896
Ran for 12550 frames Cell Events: 461 mean_circ: 0.941872
Ran for 13550 frames Cell Events: 492 mean_circ: 0.941599
Ran for 14050 frames Cell Events: 519 mean_circ: 0.941184
Ran for 14550 frames Cell Events: 543 mean_circ: 0.941498
Ran for 15050 frames Cell Events: 568 mean_circ: 0.941837
Ran for 16550 frames Cell Events: 644 mean_circ: 0.940652
Ran for 17050 frames Cell Events: 669 mean_circ: 0.940402
Ran for 19050 frames Cell Events: 759 mean_circ: 0.940012
Ran for 19550 frames Cell Events: 787 mean_circ: 0.939912
Ran for 20050 frames Cell Events: 816 mean_circ: 0.939931
Ran for 20550 frames Cell Events: 841 mean_circ: 0.938976
Ran for 21050 frames Cell Events: 865 mean_circ: 0.938781
Ran for 21550 frames Cell Events: 891 mean_circ: 0.938939
Ran for 22050 frames Cell Events: 910 mean_circ: 0.938708
Ran for 22550 frames Cell Events: 930 mean_circ: 0.938923
Ran for 23050 frames Cell Events: 949 mean_circ: 0.938733
Ran for 23550 frames Cell Events: 966 mean_circ: 0.938821
Ran for 24050 frames Cell Events: 985 mean_circ: 0.938838
Ran for 24550 frames Cell Events: 1014 mean_circ: 0.93855
Ran for 25050 frames Cell Events: 1037 mean_circ: 0.93838
Ran for 25550 frames Cell Events: 1066 mean_circ: 0.938027
Ran for 26550 frames Cell Events: 1109 mean_circ: 0.938204
Ran for 27050 frames Cell Events: 1132 mean_circ: 0.9381
Ran for 27550 frames Cell Events: 1152 mean_circ: 0.938044
Ran for 28050 frames Cell Events: 1176 mean_circ: 0.937879
Ran for 29050 frames Cell Events: 1227 mean_circ: 0.937826
Ran for 29550 frames Cell Events: 1247 mean_circ: 0.937872
Ran for 30050 frames Cell Events: 1263 mean_circ: 0.937914
Ran for 30550 frames Cell Events: 1281 mean_circ: 0.938063
Ran for 31050 frames Cell Events: 1309 mean_circ: 0.938182
Ran for 31550 frames Cell Events: 1326 mean_circ: 0.938203
Ran for 32050 frames Cell Events: 1346 mean_circ: 0.938246
Ran for 32550 frames Cell Events: 1370 mean_circ: 0.938066
Ran for 33050 frames Cell Events: 1394 mean_circ: 0.937958
Ran for 34050 frames Cell Events: 1423 mean_circ: 0.93794
Ran for 35550 frames Cell Events: 1493 mean_circ: 0.937646
Ran for 36050 frames Cell Events: 1529 mean_circ: 0.93747
Ran for 37050 frames Cell Events: 1594 mean_circ: 0.937351
Ran for 37550 frames Cell Events: 1617 mean_circ: 0.937396
Ran for 38050 frames Cell Events: 1628 mean_circ: 0.937369
Ran for 39050 frames Cell Events: 1674 mean_circ: 0.937147
Ran for 40050 frames Cell Events: 1722 mean_circ: 0.936721
Ran for 40550 frames Cell Events: 1748 mean_circ: 0.936623
Ran for 41550 frames Cell Events: 1802 mean_circ: 0.936691
Ran for 42050 frames Cell Events: 1830 mean_circ: 0.936747
Ran for 44050 frames Cell Events: 1920 mean_circ: 0.936704
Ran for 44550 frames Cell Events: 1947 mean_circ: 0.936311
Ran for 45050 frames Cell Events: 1966 mean_circ: 0.936218
Ran for 46050 frames Cell Events: 2021 mean_circ: 0.936226
Ran for 46550 frames Cell Events: 2036 mean_circ: 0.936059
Ran for 47050 frames Cell Events: 2069 mean_circ: 0.936151
Ran for 48050 frames Cell Events: 2127 mean_circ: 0.936375
Ran for 48550 frames Cell Events: 2143 mean_circ: 0.936385
Ran for 49050 frames Cell Events: 2168 mean_circ: 0.936363
Ran for 49550 frames Cell Events: 2188 mean_circ: 0.936364
Ran for 50050 frames Cell Events: 2211 mean_circ: 0.936305
Ran for 50550 frames Cell Events: 2227 mean_circ: 0.93623
Ran for 51050 frames Cell Events: 2251 mean_circ: 0.936254
Ran for 51550 frames Cell Events: 2272 mean_circ: 0.936382
Ran for 53050 frames Cell Events: 2339 mean_circ: 0.936342
Ran for 53550 frames Cell Events: 2354 mean_circ: 0.93632
Ran for 54050 frames Cell Events: 2374 mean_circ: 0.936415
Ran for 54550 frames Cell Events: 2391 mean_circ: 0.936551
Ran for 55550 frames Cell Events: 2435 mean_circ: 0.936498
Ran for 56050 frames Cell Events: 2459 mean_circ: 0.936587
Ran for 56550 frames Cell Events: 2477 mean_circ: 0.936624
Ran for 57050 frames Cell Events: 2494 mean_circ: 0.93666
Ran for 58050 frames Cell Events: 2564 mean_circ: 0.936517
Ran for 58550 frames Cell Events: 2586 mean_circ: 0.936476
Ran for 60550 frames Cell Events: 2677 mean_circ: 0.936469
Ran for 61050 frames Cell Events: 2694 mean_circ: 0.936405
Ran for 61550 frames Cell Events: 2706 mean_circ: 0.936405
Ran for 62050 frames Cell Events: 2726 mean_circ: 0.936398
Ran for 62550 frames Cell Events: 2742 mean_circ: 0.936177
Ran for 63050 frames Cell Events: 2765 mean_circ: 0.936099
Ran for 63550 frames Cell Events: 2801 mean_circ: 0.93622
Ran for 64550 frames Cell Events: 2848 mean_circ: 0.936336
Ran for 65050 frames Cell Events: 2867 mean_circ: 0.93634
Ran for 65550 frames Cell Events: 2882 mean_circ: 0.936352
Ran for 66050 frames Cell Events: 2895 mean_circ: 0.93628
Ran for 67050 frames Cell Events: 2942 mean_circ: 0.936392
Ran for 68550 frames Cell Events: 3008 mean_circ: 0.936454
Ran for 70050 frames Cell Events: 3078 mean_circ: 0.936491
Ran for 72050 frames Cell Events: 3163 mean_circ: 0.936549
Ran for 75550 frames Cell Events: 3329 mean_circ: 0.936505
Ran for 76050 frames Cell Events: 3344 mean_circ: 0.936445
Ran for 77050 frames Cell Events: 3386 mean_circ: 0.936544
Ran for 78550 frames Cell Events: 3458 mean_circ: 0.936705
Ran for 79050 frames Cell Events: 3487 mean_circ: 0.936715
Ran for 80050 frames Cell Events: 3522 mean_circ: 0.936807
Ran for 80550 frames Cell Events: 3533 mean_circ: 0.936869
Ran for 81050 frames Cell Events: 3562 mean_circ: 0.936907
Ran for 82550 frames Cell Events: 3648 mean_circ: 0.936985
Ran for 84550 frames Cell Events: 3718 mean_circ: 0.937099
Ran for 85050 frames Cell Events: 3744 mean_circ: 0.937151
Ran for 86550 frames Cell Events: 3818 mean_circ: 0.937257
Ran for 87050 frames Cell Events: 3835 mean_circ: 0.937275
Ran for 88050 frames Cell Events: 3880 mean_circ: 0.937215
Ran for 89050 frames Cell Events: 3915 mean_circ: 0.937214
Ran for 89550 frames Cell Events: 3942 mean_circ: 0.937176
Ran for 90050 frames Cell Events: 3955 mean_circ: 0.937209
Ran for 90550 frames Cell Events: 3980 mean_circ: 0.937209
Ran for 91550 frames Cell Events: 4016 mean_circ: 0.937132
Ran for 92050 frames Cell Events: 4041 mean_circ: 0.937147
Ran for 92550 frames Cell Events: 4061 mean_circ: 0.937183
Ran for 93050 frames Cell Events: 4084 mean_circ: 0.937205
Ran for 94050 frames Cell Events: 4132 mean_circ: 0.937083
Ran for 95050 frames Cell Events: 4179 mean_circ: 0.937155
Ran for 95550 frames Cell Events: 4199 mean_circ: 0.937067
Ran for 98050 frames Cell Events: 4315 mean_circ: 0.937169
Ran for 99050 frames Cell Events: 4366 mean_circ: 0.937225
Ran for 99550 frames Cell Events: 4388 mean_circ: 0.937173
Ran for 100050 frames Cell Events: 4410 mean_circ: 0.937005
Ran for 101550 frames Cell Events: 4445 mean_circ: 0.937105
Ran for 103050 frames Cell Events: 4509 mean_circ: 0.937213
Ran for 104050 frames Cell Events: 4555 mean_circ: 0.937156
Ran for 104550 frames Cell Events: 4573 mean_circ: 0.937191
Ran for 105550 frames Cell Events: 4621 mean_circ: 0.937052
Ran for 106050 frames Cell Events: 4634 mean_circ: 0.937067
Ran for 107550 frames Cell Events: 4706 mean_circ: 0.937017
Ran for 108050 frames Cell Events: 4737 mean_circ: 0.936997
Ran for 109050 frames Cell Events: 4783 mean_circ: 0.936972
Ran for 110550 frames Cell Events: 4818 mean_circ: 0.936997
Ran for 111050 frames Cell Events: 4844 mean_circ: 0.936975
Ran for 113050 frames Cell Events: 4914 mean_circ: 0.937017
Ran for 113550 frames Cell Events: 4934 mean_circ: 0.93702
Ran for 114050 frames Cell Events: 4948 mean_circ: 0.936935
Ran for 115550 frames Cell Events: 4994 mean_circ: 0.936964
Ran for 116050 frames Cell Events: 5014 mean_circ: 0.936981
Ran for 116550 frames Cell Events: 5028 mean_circ: 0.936973
Ran for 117550 frames Cell Events: 5068 mean_circ: 0.937
Ran for 118550 frames Cell Events: 5094 mean_circ: 0.937
Ran for 119050 frames Cell Events: 5123 mean_circ: 0.936917
Ran for 120050 frames Cell Events: 5171 mean_circ: 0.936972
Ran for 121050 frames Cell Events: 5223 mean_circ: 0.936976
Ran for 121550 frames Cell Events: 5237 mean_circ: 0.936959
Ran for 122050 frames Cell Events: 5267 mean_circ: 0.936927
Ran for 122550 frames Cell Events: 5297 mean_circ: 0.936907
Ran for 124050 frames Cell Events: 5367 mean_circ: 0.93696
Ran for 125050 frames Cell Events: 5389 mean_circ: 0.936816
Ran for 125550 frames Cell Events: 5411 mean_circ: 0.936805
Ran for 126550 frames Cell Events: 5463 mean_circ: 0.936793
Ran for 128050 frames Cell Events: 5533 mean_circ: 0.936813
Ran for 129050 frames Cell Events: 5593 mean_circ: 0.936833
Ran for 130050 frames Cell Events: 5647 mean_circ: 0.936948
Ran for 130550 frames Cell Events: 5669 mean_circ: 0.936994
Ran for 132050 frames Cell Events: 5745 mean_circ: 0.936947
Ran for 132550 frames Cell Events: 5762 mean_circ: 0.936958
Ran for 133550 frames Cell Events: 5808 mean_circ: 0.937006
Ran for 134550 frames Cell Events: 5860 mean_circ: 0.937006
Ran for 136550 frames Cell Events: 5959 mean_circ: 0.937043
Ran for 139050 frames Cell Events: 6075 mean_circ: 0.936982
Ran for 140550 frames Cell Events: 6150 mean_circ: 0.937005
Ran for 141050 frames Cell Events: 6165 mean_circ: 0.937015
Ran for 141550 frames Cell Events: 6195 mean_circ: 0.937015
Ran for 144050 frames Cell Events: 6293 mean_circ: 0.937003
Ran for 144550 frames Cell Events: 6310 mean_circ: 0.937038
Ran for 146050 frames Cell Events: 6367 mean_circ: 0.93705
Ran for 147050 frames Cell Events: 6427 mean_circ: 0.93701
Ran for 147550 frames Cell Events: 6452 mean_circ: 0.937028
Ran for 148050 frames Cell Events: 6473 mean_circ: 0.937028
Ran for 149050 frames Cell Events: 6514 mean_circ: 0.937075
Ran for 149550 frames Cell Events: 6528 mean_circ: 0.937105
Ran for 150050 frames Cell Events: 6545 mean_circ: 0.937134
Ran for 150550 frames Cell Events: 6571 mean_circ: 0.937175
Ran for 151550 frames Cell Events: 6609 mean_circ: 0.937215
Ran for 152050 frames Cell Events: 6633 mean_circ: 0.937217
Ran for 152550 frames Cell Events: 6658 mean_circ: 0.937175
Ran for 154050 frames Cell Events: 6728 mean_circ: 0.93716
Ran for 156050 frames Cell Events: 6823 mean_circ: 0.937028
Ran for 156550 frames Cell Events: 6840 mean_circ: 0.936958
Ran for 157550 frames Cell Events: 6884 mean_circ: 0.93696
Ran for 158550 frames Cell Events: 6930 mean_circ: 0.936945
Ran for 160050 frames Cell Events: 6985 mean_circ: 0.936978
Ran for 160550 frames Cell Events: 7003 mean_circ: 0.936903
Ran for 161050 frames Cell Events: 7023 mean_circ: 0.936914
Ran for 162050 frames Cell Events: 7062 mean_circ: 0.936896
Ran for 162550 frames Cell Events: 7094 mean_circ: 0.936866
Ran for 163050 frames Cell Events: 7112 mean_circ: 0.936841
Ran for 163550 frames Cell Events: 7139 mean_circ: 0.936854
Ran for 164550 frames Cell Events: 7184 mean_circ: 0.936836
Ran for 167550 frames Cell Events: 7333 mean_circ: 0.936761
Ran for 168550 frames Cell Events: 7377 mean_circ: 0.936725
Ran for 169050 frames Cell Events: 7403 mean_circ: 0.936737
Ran for 169550 frames Cell Events: 7426 mean_circ: 0.936777
Ran for 171050 frames Cell Events: 7488 mean_circ: 0.93676
Ran for 171550 frames Cell Events: 7507 mean_circ: 0.93676
Ran for 173050 frames Cell Events: 7573 mean_circ: 0.936769
Ran for 173550 frames Cell Events: 7603 mean_circ: 0.936784
Ran for 174050 frames Cell Events: 7623 mean_circ: 0.936784
Ran for 175050 frames Cell Events: 7676 mean_circ: 0.936734
Ran for 176050 frames Cell Events: 7725 mean_circ: 0.936787
Ran for 177050 frames Cell Events: 7799 mean_circ: 0.936787
Ran for 177550 frames Cell Events: 7819 mean_circ: 0.9368
Ran for 178050 frames Cell Events: 7842 mean_circ: 0.936801
Ran for 178550 frames Cell Events: 7870 mean_circ: 0.936794
Ran for 180050 frames Cell Events: 7932 mean_circ: 0.936773
Ran for 182050 frames Cell Events: 8012 mean_circ: 0.936737
Ran for 183550 frames Cell Events: 8079 mean_circ: 0.936734
Ran for 184550 frames Cell Events: 8129 mean_circ: 0.936747
End this measurment..
Stopping time: 1.68373e+009
****************************************
Measurement finished
Mean Circularitiy: 0.936757
Mean Area: 591.615
Mean FL-1 intensity: 3467.95
Mean FL-2 intensity: 3619.11
Mean FL-3 intensity: 0
frames processed: 180387
Total Time: 92360.2ms
Number of Runs (new bg img) 0
Frames collected: 184594
Skipped frames: 184271
Rescued Frames: 5112698
Samples DAQ: 46148587
Samples written: 92297086
Samples per Channel: 46148543
FPS: 1998.63
Samples per second: 499658
Expected samples/s: 500000
Events: 8130
Events per second: 88.0249
jumped back (PMT): 23
#Frames read, frequency
# 0 frames :279115
# 1 frames :75237
# 2 frames :3645
# 3 frames :1417
# 4 frames :791
# 5 frames :406
# 6 frames :1746
# 7 frames :1395
# 8 frames :1137
# 9 frames :761
# 10 frames :515
# 11 frames :490
# 12 frames :389
# 13 frames :281
# 14 frames :252
# 15 frames :223
# 16 frames :173
# 17 frames :141
# 18 frames :106
# 19 frames :95
# 20 frames :106
# 21 frames :75
# 22 frames :76
# 23 frames :54
# 24 frames :42
# 25 frames :44
# 26 frames :29
# 27 frames :32
# 28 frames :27
# 29 frames :19
# 30 frames :18
# 31 frames :18
# 32 frames :16
# 33 frames :22
# 34 frames :12
# 35 frames :8
# 36 frames :12
# 37 frames :6
# 38 frames :6
# 39 frames :12
# 40 frames :5
# 41 frames :9
# 42 frames :4
# 43 frames :4
# 44 frames :4
# 45 frames :4
# 46 frames :1
# 47 frames :2
# 49 frames :4
# 51 frames :2
# 52 frames :3
# 54 frames :1
# 55 frames :2
# 56 frames :2
# 57 frames :3
# 58 frames :1
# 59 frames :2
# 61 frames :2
# 62 frames :1
# 64 frames :1
# 65 frames :2
# 68 frames :1
# 74 frames :1
# 85 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9768
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0