Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.39.01
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d871f9ddddb40fae34ac2a442fe0d1e1",
"name": "M1_20170604_1541_dsRNA_E4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:39:21"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8052 (max 8053) in '8052'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1618
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75433e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 15 mean_circ: 0.952303
Ran for 1050 frames Cell Events: 35 mean_circ: 0.942708
Ran for 1550 frames Cell Events: 57 mean_circ: 0.945716
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Ran for 2550 frames Cell Events: 85 mean_circ: 0.945838
Ran for 3050 frames Cell Events: 104 mean_circ: 0.947017
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Ran for 67050 frames Cell Events: 1766 mean_circ: 0.941885
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Ran for 72050 frames Cell Events: 1925 mean_circ: 0.941852
Ran for 72550 frames Cell Events: 1946 mean_circ: 0.941865
Ran for 73050 frames Cell Events: 1960 mean_circ: 0.941903
Ran for 73550 frames Cell Events: 1972 mean_circ: 0.94189
Ran for 74050 frames Cell Events: 1993 mean_circ: 0.941865
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Ran for 83050 frames Cell Events: 2254 mean_circ: 0.941876
Ran for 83550 frames Cell Events: 2272 mean_circ: 0.941826
Ran for 84050 frames Cell Events: 2284 mean_circ: 0.941863
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Ran for 85550 frames Cell Events: 2305 mean_circ: 0.941893
Ran for 86050 frames Cell Events: 2319 mean_circ: 0.941949
Ran for 86550 frames Cell Events: 2337 mean_circ: 0.942027
Ran for 87050 frames Cell Events: 2343 mean_circ: 0.94205
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Ran for 91050 frames Cell Events: 2457 mean_circ: 0.942203
Ran for 91550 frames Cell Events: 2467 mean_circ: 0.942215
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Ran for 103050 frames Cell Events: 2855 mean_circ: 0.942249
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Ran for 104550 frames Cell Events: 2889 mean_circ: 0.942097
Ran for 106550 frames Cell Events: 2939 mean_circ: 0.942154
Ran for 107050 frames Cell Events: 2953 mean_circ: 0.942067
Ran for 108050 frames Cell Events: 2992 mean_circ: 0.942142
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Ran for 121050 frames Cell Events: 3390 mean_circ: 0.942589
Ran for 121550 frames Cell Events: 3408 mean_circ: 0.942579
Ran for 122050 frames Cell Events: 3415 mean_circ: 0.942599
Ran for 122550 frames Cell Events: 3424 mean_circ: 0.94261
Ran for 123050 frames Cell Events: 3437 mean_circ: 0.942508
Ran for 123550 frames Cell Events: 3450 mean_circ: 0.942455
Ran for 124050 frames Cell Events: 3462 mean_circ: 0.942483
Ran for 124550 frames Cell Events: 3476 mean_circ: 0.942511
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Ran for 126050 frames Cell Events: 3528 mean_circ: 0.942555
Ran for 126550 frames Cell Events: 3539 mean_circ: 0.942582
Ran for 127550 frames Cell Events: 3568 mean_circ: 0.94253
Ran for 128050 frames Cell Events: 3578 mean_circ: 0.942511
Ran for 128550 frames Cell Events: 3590 mean_circ: 0.942557
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Ran for 131050 frames Cell Events: 3651 mean_circ: 0.942643
Ran for 132050 frames Cell Events: 3681 mean_circ: 0.942715
Ran for 132550 frames Cell Events: 3695 mean_circ: 0.942675
Ran for 134050 frames Cell Events: 3738 mean_circ: 0.942657
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Ran for 173550 frames Cell Events: 4884 mean_circ: 0.942801
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Ran for 181550 frames Cell Events: 5097 mean_circ: 0.942553
Ran for 182050 frames Cell Events: 5110 mean_circ: 0.942559
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Ran for 183550 frames Cell Events: 5156 mean_circ: 0.94254
Ran for 184550 frames Cell Events: 5199 mean_circ: 0.942538
Ran for 185050 frames Cell Events: 5209 mean_circ: 0.942523
Ran for 186050 frames Cell Events: 5232 mean_circ: 0.942583
Ran for 187550 frames Cell Events: 5286 mean_circ: 0.942557
Ran for 188050 frames Cell Events: 5301 mean_circ: 0.942612
Ran for 189050 frames Cell Events: 5314 mean_circ: 0.942567
Ran for 189550 frames Cell Events: 5333 mean_circ: 0.942554
Ran for 190550 frames Cell Events: 5356 mean_circ: 0.942486
Ran for 191550 frames Cell Events: 5393 mean_circ: 0.942513
Ran for 192050 frames Cell Events: 5417 mean_circ: 0.942607
Ran for 193050 frames Cell Events: 5439 mean_circ: 0.942628
Ran for 195050 frames Cell Events: 5511 mean_circ: 0.942596
Ran for 195550 frames Cell Events: 5521 mean_circ: 0.942607
Ran for 196050 frames Cell Events: 5536 mean_circ: 0.942618
Ran for 197050 frames Cell Events: 5570 mean_circ: 0.942635
Ran for 197550 frames Cell Events: 5595 mean_circ: 0.942642
Ran for 198550 frames Cell Events: 5625 mean_circ: 0.942686
Ran for 199050 frames Cell Events: 5647 mean_circ: 0.942662
Ran for 199550 frames Cell Events: 5658 mean_circ: 0.942667
Ran for 200550 frames Cell Events: 5688 mean_circ: 0.94265
Ran for 201050 frames Cell Events: 5703 mean_circ: 0.942645
Ran for 201550 frames Cell Events: 5721 mean_circ: 0.942644
Ran for 203550 frames Cell Events: 5784 mean_circ: 0.942726
Ran for 204050 frames Cell Events: 5800 mean_circ: 0.942719
Ran for 205050 frames Cell Events: 5824 mean_circ: 0.942699
Ran for 206050 frames Cell Events: 5861 mean_circ: 0.942703
Ran for 206550 frames Cell Events: 5873 mean_circ: 0.942634
Ran for 207050 frames Cell Events: 5887 mean_circ: 0.942653
Ran for 208050 frames Cell Events: 5915 mean_circ: 0.942693
Ran for 208550 frames Cell Events: 5928 mean_circ: 0.942697
Ran for 209050 frames Cell Events: 5938 mean_circ: 0.942711
Ran for 209550 frames Cell Events: 5953 mean_circ: 0.942719
Ran for 210050 frames Cell Events: 5966 mean_circ: 0.942686
Ran for 210550 frames Cell Events: 5982 mean_circ: 0.942724
Ran for 211050 frames Cell Events: 5998 mean_circ: 0.942717
Ran for 211550 frames Cell Events: 6017 mean_circ: 0.942732
Ran for 212050 frames Cell Events: 6028 mean_circ: 0.942732
Ran for 212550 frames Cell Events: 6042 mean_circ: 0.942712
Ran for 213050 frames Cell Events: 6058 mean_circ: 0.942652
Ran for 214050 frames Cell Events: 6090 mean_circ: 0.942629
Ran for 214550 frames Cell Events: 6106 mean_circ: 0.942617
Ran for 215050 frames Cell Events: 6116 mean_circ: 0.942615
Ran for 215550 frames Cell Events: 6133 mean_circ: 0.942611
Ran for 216050 frames Cell Events: 6151 mean_circ: 0.942585
Ran for 216550 frames Cell Events: 6163 mean_circ: 0.942599
Ran for 217050 frames Cell Events: 6175 mean_circ: 0.942637
Ran for 217550 frames Cell Events: 6184 mean_circ: 0.942676
Ran for 218050 frames Cell Events: 6189 mean_circ: 0.942668
Ran for 218550 frames Cell Events: 6192 mean_circ: 0.94266
Ran for 219050 frames Cell Events: 6205 mean_circ: 0.942629
Ran for 220050 frames Cell Events: 6236 mean_circ: 0.942608
Ran for 220550 frames Cell Events: 6249 mean_circ: 0.942604
Ran for 221050 frames Cell Events: 6268 mean_circ: 0.942613
Ran for 222050 frames Cell Events: 6300 mean_circ: 0.94266
Ran for 223050 frames Cell Events: 6327 mean_circ: 0.942681
Ran for 223550 frames Cell Events: 6342 mean_circ: 0.942664
Ran for 226050 frames Cell Events: 6432 mean_circ: 0.942644
Ran for 227050 frames Cell Events: 6468 mean_circ: 0.942615
Ran for 227550 frames Cell Events: 6476 mean_circ: 0.942631
Ran for 228050 frames Cell Events: 6488 mean_circ: 0.94264
Ran for 228550 frames Cell Events: 6507 mean_circ: 0.942648
Ran for 229050 frames Cell Events: 6514 mean_circ: 0.942665
Ran for 229550 frames Cell Events: 6529 mean_circ: 0.942672
Ran for 230050 frames Cell Events: 6538 mean_circ: 0.94266
Ran for 230550 frames Cell Events: 6550 mean_circ: 0.942684
Ran for 231050 frames Cell Events: 6566 mean_circ: 0.942662
Ran for 232050 frames Cell Events: 6592 mean_circ: 0.942618
Ran for 232550 frames Cell Events: 6606 mean_circ: 0.942603
Ran for 233050 frames Cell Events: 6626 mean_circ: 0.942551
Ran for 233550 frames Cell Events: 6635 mean_circ: 0.942534
Ran for 234050 frames Cell Events: 6643 mean_circ: 0.942537
Ran for 234550 frames Cell Events: 6657 mean_circ: 0.942524
Ran for 235050 frames Cell Events: 6668 mean_circ: 0.942539
Ran for 237550 frames Cell Events: 6738 mean_circ: 0.942523
Ran for 238050 frames Cell Events: 6754 mean_circ: 0.942523
Ran for 239050 frames Cell Events: 6780 mean_circ: 0.942468
Ran for 240050 frames Cell Events: 6807 mean_circ: 0.942476
Ran for 240550 frames Cell Events: 6814 mean_circ: 0.942494
Ran for 241050 frames Cell Events: 6829 mean_circ: 0.942489
Ran for 241550 frames Cell Events: 6843 mean_circ: 0.942467
Ran for 242050 frames Cell Events: 6852 mean_circ: 0.942483
Ran for 242550 frames Cell Events: 6870 mean_circ: 0.942492
Ran for 243050 frames Cell Events: 6894 mean_circ: 0.942537
Ran for 243550 frames Cell Events: 6907 mean_circ: 0.942582
Ran for 244050 frames Cell Events: 6920 mean_circ: 0.942588
Ran for 244550 frames Cell Events: 6928 mean_circ: 0.942601
Ran for 245050 frames Cell Events: 6938 mean_circ: 0.94259
Ran for 246050 frames Cell Events: 6968 mean_circ: 0.942589
Ran for 246550 frames Cell Events: 6982 mean_circ: 0.942544
Ran for 247050 frames Cell Events: 7010 mean_circ: 0.942529
Ran for 247550 frames Cell Events: 7024 mean_circ: 0.942553
Ran for 248050 frames Cell Events: 7036 mean_circ: 0.942568
Ran for 249050 frames Cell Events: 7064 mean_circ: 0.942587
Ran for 250050 frames Cell Events: 7096 mean_circ: 0.942539
Ran for 250550 frames Cell Events: 7110 mean_circ: 0.942547
Ran for 251050 frames Cell Events: 7125 mean_circ: 0.942497
Ran for 251550 frames Cell Events: 7144 mean_circ: 0.942495
Ran for 252050 frames Cell Events: 7155 mean_circ: 0.942517
Ran for 252550 frames Cell Events: 7174 mean_circ: 0.942528
Ran for 254050 frames Cell Events: 7235 mean_circ: 0.942453
Ran for 254550 frames Cell Events: 7250 mean_circ: 0.942444
Ran for 255050 frames Cell Events: 7269 mean_circ: 0.942413
Ran for 258050 frames Cell Events: 7360 mean_circ: 0.942391
Ran for 258550 frames Cell Events: 7375 mean_circ: 0.942391
Ran for 259050 frames Cell Events: 7389 mean_circ: 0.942355
Ran for 261050 frames Cell Events: 7447 mean_circ: 0.942346
Ran for 261550 frames Cell Events: 7471 mean_circ: 0.942357
Ran for 262050 frames Cell Events: 7486 mean_circ: 0.942366
Ran for 262550 frames Cell Events: 7504 mean_circ: 0.942372
Ran for 264550 frames Cell Events: 7563 mean_circ: 0.94242
Ran for 265050 frames Cell Events: 7580 mean_circ: 0.942424
Ran for 266050 frames Cell Events: 7603 mean_circ: 0.942458
Ran for 266550 frames Cell Events: 7616 mean_circ: 0.942413
Ran for 267050 frames Cell Events: 7625 mean_circ: 0.942397
Ran for 268050 frames Cell Events: 7636 mean_circ: 0.942373
Ran for 269050 frames Cell Events: 7674 mean_circ: 0.94233
Ran for 269550 frames Cell Events: 7684 mean_circ: 0.942354
Ran for 270050 frames Cell Events: 7706 mean_circ: 0.942377
Ran for 271550 frames Cell Events: 7749 mean_circ: 0.94233
Ran for 272550 frames Cell Events: 7787 mean_circ: 0.942327
Ran for 273050 frames Cell Events: 7805 mean_circ: 0.942335
Ran for 274550 frames Cell Events: 7850 mean_circ: 0.942404
Ran for 275050 frames Cell Events: 7859 mean_circ: 0.942424
Ran for 275550 frames Cell Events: 7872 mean_circ: 0.942416
Ran for 277050 frames Cell Events: 7918 mean_circ: 0.942379
Ran for 278050 frames Cell Events: 7959 mean_circ: 0.942363
Ran for 278550 frames Cell Events: 7980 mean_circ: 0.942381
Ran for 279050 frames Cell Events: 8004 mean_circ: 0.942301
Ran for 279550 frames Cell Events: 8019 mean_circ: 0.942306
Ran for 280050 frames Cell Events: 8033 mean_circ: 0.942313
Ran for 280550 frames Cell Events: 8048 mean_circ: 0.942321
End this measurment..
Stopping time: 1.75447e+009
****************************************
Measurement finished
Mean Circularitiy: 0.942321
Mean Area: 602.912
Mean FL-1 intensity: 3246.22
Mean FL-2 intensity: 3726
Mean FL-3 intensity: 0
frames processed: 278251
Total Time: 140395ms
Number of Runs (new bg img) 0
Frames collected: 280661
Skipped frames: 59873
Rescued Frames: 1601494
Samples DAQ: 70165280
Samples written: 140330438
Samples per Channel: 70165219
FPS: 1999.08
Samples per second: 499771
Expected samples/s: 500000
Events: 8053
Events per second: 57.3596
jumped back (PMT): 5232
#Frames read, frequency
# 0 frames :390536
# 1 frames :151573
# 2 frames :5274
# 3 frames :2187
# 4 frames :1224
# 5 frames :591
# 6 frames :1550
# 7 frames :1841
# 8 frames :1562
# 9 frames :970
# 10 frames :822
# 11 frames :610
# 12 frames :491
# 13 frames :333
# 14 frames :279
# 15 frames :247
# 16 frames :202
# 17 frames :169
# 18 frames :149
# 19 frames :105
# 20 frames :88
# 21 frames :72
# 22 frames :77
# 23 frames :60
# 24 frames :27
# 25 frames :27
# 26 frames :24
# 27 frames :35
# 28 frames :26
# 29 frames :23
# 30 frames :13
# 31 frames :9
# 32 frames :24
# 33 frames :7
# 34 frames :10
# 35 frames :9
# 36 frames :6
# 37 frames :4
# 38 frames :10
# 39 frames :4
# 40 frames :4
# 41 frames :6
# 42 frames :2
# 43 frames :2
# 45 frames :1
# 46 frames :2
# 47 frames :2
# 48 frames :1
# 50 frames :1
# 52 frames :2
# 54 frames :3
# 55 frames :1
# 57 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1618
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0