Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.39.29
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a0f181617a5b65f2d8f7e8f2456dd10e",
"name": "M1_20170604_1545_dsRNA_E4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:39:49"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8070 (max 8071) in '8070'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1702
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75455e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 55 mean_circ: 0.928531
Ran for 2050 frames Cell Events: 67 mean_circ: 0.93204
Ran for 2550 frames Cell Events: 78 mean_circ: 0.9329
Ran for 3050 frames Cell Events: 85 mean_circ: 0.935554
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Ran for 4550 frames Cell Events: 140 mean_circ: 0.934865
Ran for 5050 frames Cell Events: 155 mean_circ: 0.935386
Ran for 5550 frames Cell Events: 169 mean_circ: 0.935959
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Ran for 7550 frames Cell Events: 222 mean_circ: 0.9362
Ran for 8050 frames Cell Events: 240 mean_circ: 0.936908
Ran for 9550 frames Cell Events: 334 mean_circ: 0.934617
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Ran for 81050 frames Cell Events: 2643 mean_circ: 0.933133
Ran for 81550 frames Cell Events: 2662 mean_circ: 0.933044
Ran for 82050 frames Cell Events: 2683 mean_circ: 0.933018
Ran for 83050 frames Cell Events: 2716 mean_circ: 0.933161
Ran for 86050 frames Cell Events: 2806 mean_circ: 0.932909
Ran for 86550 frames Cell Events: 2825 mean_circ: 0.932909
Ran for 87050 frames Cell Events: 2833 mean_circ: 0.932949
Ran for 87550 frames Cell Events: 2851 mean_circ: 0.932785
Ran for 88050 frames Cell Events: 2871 mean_circ: 0.932739
Ran for 88550 frames Cell Events: 2895 mean_circ: 0.932681
Ran for 89050 frames Cell Events: 2907 mean_circ: 0.932639
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Ran for 90050 frames Cell Events: 2940 mean_circ: 0.932777
Ran for 90550 frames Cell Events: 2950 mean_circ: 0.932734
Ran for 91050 frames Cell Events: 2970 mean_circ: 0.932648
Ran for 93050 frames Cell Events: 3033 mean_circ: 0.932661
Ran for 93550 frames Cell Events: 3058 mean_circ: 0.932625
Ran for 94050 frames Cell Events: 3072 mean_circ: 0.932681
Ran for 95050 frames Cell Events: 3110 mean_circ: 0.932633
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Ran for 96550 frames Cell Events: 3153 mean_circ: 0.932659
Ran for 97050 frames Cell Events: 3175 mean_circ: 0.932701
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Ran for 98050 frames Cell Events: 3199 mean_circ: 0.932729
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Ran for 100050 frames Cell Events: 3264 mean_circ: 0.932668
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Ran for 107050 frames Cell Events: 3484 mean_circ: 0.932689
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Ran for 111050 frames Cell Events: 3599 mean_circ: 0.932653
Ran for 112050 frames Cell Events: 3627 mean_circ: 0.932606
Ran for 112550 frames Cell Events: 3638 mean_circ: 0.932572
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Ran for 120050 frames Cell Events: 3860 mean_circ: 0.932724
Ran for 120550 frames Cell Events: 3874 mean_circ: 0.932698
Ran for 121050 frames Cell Events: 3895 mean_circ: 0.932734
Ran for 122050 frames Cell Events: 3932 mean_circ: 0.932794
Ran for 123550 frames Cell Events: 3986 mean_circ: 0.932628
Ran for 124050 frames Cell Events: 3993 mean_circ: 0.932602
Ran for 124550 frames Cell Events: 4002 mean_circ: 0.932629
Ran for 125050 frames Cell Events: 4023 mean_circ: 0.932653
Ran for 125550 frames Cell Events: 4047 mean_circ: 0.932646
Ran for 126050 frames Cell Events: 4064 mean_circ: 0.932688
Ran for 127050 frames Cell Events: 4100 mean_circ: 0.932624
Ran for 128050 frames Cell Events: 4140 mean_circ: 0.932618
Ran for 129050 frames Cell Events: 4179 mean_circ: 0.93255
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Ran for 131050 frames Cell Events: 4264 mean_circ: 0.93262
Ran for 132050 frames Cell Events: 4294 mean_circ: 0.93272
Ran for 132550 frames Cell Events: 4307 mean_circ: 0.932746
Ran for 133550 frames Cell Events: 4346 mean_circ: 0.932772
Ran for 134050 frames Cell Events: 4359 mean_circ: 0.932753
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Ran for 141550 frames Cell Events: 4572 mean_circ: 0.932639
Ran for 142550 frames Cell Events: 4601 mean_circ: 0.932621
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Ran for 143550 frames Cell Events: 4633 mean_circ: 0.932633
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Ran for 163550 frames Cell Events: 5280 mean_circ: 0.932527
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Ran for 173050 frames Cell Events: 5580 mean_circ: 0.932688
Ran for 173550 frames Cell Events: 5590 mean_circ: 0.932662
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Ran for 176050 frames Cell Events: 5657 mean_circ: 0.932636
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Ran for 182550 frames Cell Events: 5900 mean_circ: 0.932443
Ran for 183050 frames Cell Events: 5919 mean_circ: 0.932471
Ran for 183550 frames Cell Events: 5938 mean_circ: 0.932433
Ran for 184050 frames Cell Events: 5958 mean_circ: 0.932469
Ran for 185050 frames Cell Events: 5986 mean_circ: 0.932454
Ran for 187050 frames Cell Events: 6058 mean_circ: 0.93249
Ran for 188050 frames Cell Events: 6093 mean_circ: 0.932352
Ran for 188550 frames Cell Events: 6102 mean_circ: 0.932303
Ran for 189050 frames Cell Events: 6119 mean_circ: 0.932281
Ran for 190050 frames Cell Events: 6161 mean_circ: 0.93223
Ran for 190550 frames Cell Events: 6179 mean_circ: 0.9322
Ran for 191050 frames Cell Events: 6188 mean_circ: 0.932206
Ran for 191550 frames Cell Events: 6203 mean_circ: 0.932214
Ran for 192550 frames Cell Events: 6234 mean_circ: 0.932206
Ran for 193050 frames Cell Events: 6257 mean_circ: 0.932144
Ran for 193550 frames Cell Events: 6267 mean_circ: 0.932155
Ran for 194050 frames Cell Events: 6285 mean_circ: 0.932165
Ran for 194550 frames Cell Events: 6311 mean_circ: 0.932185
Ran for 195050 frames Cell Events: 6319 mean_circ: 0.932184
Ran for 196050 frames Cell Events: 6342 mean_circ: 0.932177
Ran for 196550 frames Cell Events: 6355 mean_circ: 0.932207
Ran for 197050 frames Cell Events: 6370 mean_circ: 0.932225
Ran for 197550 frames Cell Events: 6386 mean_circ: 0.932257
Ran for 198050 frames Cell Events: 6398 mean_circ: 0.932278
Ran for 199050 frames Cell Events: 6443 mean_circ: 0.932299
Ran for 199550 frames Cell Events: 6461 mean_circ: 0.932307
Ran for 200050 frames Cell Events: 6478 mean_circ: 0.932267
Ran for 201050 frames Cell Events: 6508 mean_circ: 0.932224
Ran for 201550 frames Cell Events: 6526 mean_circ: 0.932224
Ran for 202050 frames Cell Events: 6545 mean_circ: 0.932221
Ran for 202550 frames Cell Events: 6557 mean_circ: 0.932262
Ran for 203550 frames Cell Events: 6590 mean_circ: 0.932287
Ran for 204050 frames Cell Events: 6608 mean_circ: 0.932312
Ran for 204550 frames Cell Events: 6622 mean_circ: 0.93231
Ran for 205050 frames Cell Events: 6640 mean_circ: 0.932349
Ran for 205550 frames Cell Events: 6654 mean_circ: 0.932338
Ran for 206050 frames Cell Events: 6664 mean_circ: 0.93236
Ran for 207050 frames Cell Events: 6698 mean_circ: 0.93236
Ran for 207550 frames Cell Events: 6714 mean_circ: 0.932359
Ran for 208050 frames Cell Events: 6731 mean_circ: 0.932373
Ran for 208550 frames Cell Events: 6745 mean_circ: 0.932387
Ran for 209050 frames Cell Events: 6753 mean_circ: 0.932387
Ran for 210050 frames Cell Events: 6796 mean_circ: 0.932398
Ran for 211050 frames Cell Events: 6830 mean_circ: 0.932354
Ran for 211550 frames Cell Events: 6848 mean_circ: 0.932364
Ran for 212550 frames Cell Events: 6874 mean_circ: 0.932383
Ran for 213550 frames Cell Events: 6903 mean_circ: 0.932421
Ran for 214050 frames Cell Events: 6919 mean_circ: 0.932422
Ran for 214550 frames Cell Events: 6935 mean_circ: 0.932412
Ran for 215550 frames Cell Events: 6965 mean_circ: 0.932425
Ran for 216050 frames Cell Events: 6988 mean_circ: 0.932442
Ran for 216550 frames Cell Events: 7000 mean_circ: 0.932449
Ran for 217050 frames Cell Events: 7015 mean_circ: 0.932443
Ran for 217550 frames Cell Events: 7039 mean_circ: 0.932452
Ran for 218050 frames Cell Events: 7055 mean_circ: 0.932488
Ran for 218550 frames Cell Events: 7068 mean_circ: 0.932502
Ran for 219050 frames Cell Events: 7094 mean_circ: 0.932523
Ran for 220050 frames Cell Events: 7128 mean_circ: 0.932541
Ran for 220550 frames Cell Events: 7144 mean_circ: 0.93254
Ran for 221550 frames Cell Events: 7181 mean_circ: 0.932525
Ran for 222050 frames Cell Events: 7202 mean_circ: 0.93257
Ran for 222550 frames Cell Events: 7222 mean_circ: 0.932563
Ran for 223050 frames Cell Events: 7241 mean_circ: 0.93251
Ran for 224050 frames Cell Events: 7262 mean_circ: 0.93247
Ran for 224550 frames Cell Events: 7280 mean_circ: 0.932458
Ran for 226050 frames Cell Events: 7314 mean_circ: 0.93244
Ran for 226550 frames Cell Events: 7328 mean_circ: 0.932445
Ran for 227050 frames Cell Events: 7342 mean_circ: 0.932396
Ran for 227550 frames Cell Events: 7355 mean_circ: 0.932412
Ran for 228050 frames Cell Events: 7366 mean_circ: 0.932398
Ran for 228550 frames Cell Events: 7380 mean_circ: 0.932416
Ran for 229050 frames Cell Events: 7394 mean_circ: 0.932381
Ran for 230050 frames Cell Events: 7428 mean_circ: 0.932366
Ran for 231050 frames Cell Events: 7457 mean_circ: 0.932367
Ran for 231550 frames Cell Events: 7475 mean_circ: 0.932343
Ran for 232050 frames Cell Events: 7489 mean_circ: 0.932354
Ran for 234050 frames Cell Events: 7568 mean_circ: 0.932277
Ran for 234550 frames Cell Events: 7592 mean_circ: 0.932277
Ran for 236550 frames Cell Events: 7656 mean_circ: 0.93225
Ran for 237050 frames Cell Events: 7670 mean_circ: 0.932251
Ran for 237550 frames Cell Events: 7686 mean_circ: 0.932267
Ran for 239050 frames Cell Events: 7726 mean_circ: 0.932299
Ran for 240550 frames Cell Events: 7776 mean_circ: 0.932302
Ran for 241050 frames Cell Events: 7795 mean_circ: 0.932307
Ran for 242050 frames Cell Events: 7823 mean_circ: 0.932329
Ran for 242550 frames Cell Events: 7838 mean_circ: 0.932356
Ran for 243050 frames Cell Events: 7854 mean_circ: 0.932348
Ran for 243550 frames Cell Events: 7876 mean_circ: 0.93235
Ran for 244050 frames Cell Events: 7894 mean_circ: 0.932367
Ran for 244550 frames Cell Events: 7901 mean_circ: 0.932367
Ran for 245050 frames Cell Events: 7917 mean_circ: 0.932372
Ran for 246050 frames Cell Events: 7949 mean_circ: 0.932266
Ran for 246550 frames Cell Events: 7965 mean_circ: 0.932298
Ran for 247050 frames Cell Events: 7984 mean_circ: 0.932275
Ran for 247550 frames Cell Events: 8004 mean_circ: 0.932291
Ran for 248050 frames Cell Events: 8020 mean_circ: 0.932284
End this measurment..
Stopping time: 1.75467e+009
****************************************
Measurement finished
Mean Circularitiy: 0.932267
Mean Area: 597.839
Mean FL-1 intensity: 3154.52
Mean FL-2 intensity: 4139.93
Mean FL-3 intensity: 0
frames processed: 246866
Total Time: 124669ms
Number of Runs (new bg img) 0
Frames collected: 249221
Skipped frames: 62228
Rescued Frames: 1694302
Samples DAQ: 62305387
Samples written: 124610552
Samples per Channel: 62305276
FPS: 1999.05
Samples per second: 499764
Expected samples/s: 500000
Events: 8071
Events per second: 64.7392
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :344356
# 1 frames :135804
# 2 frames :5319
# 3 frames :1884
# 4 frames :1152
# 5 frames :498
# 6 frames :1737
# 7 frames :2082
# 8 frames :1656
# 9 frames :1037
# 10 frames :586
# 11 frames :472
# 12 frames :459
# 13 frames :284
# 14 frames :194
# 15 frames :186
# 16 frames :147
# 17 frames :113
# 18 frames :91
# 19 frames :74
# 20 frames :54
# 21 frames :38
# 22 frames :39
# 23 frames :30
# 24 frames :23
# 25 frames :12
# 26 frames :16
# 27 frames :11
# 28 frames :4
# 29 frames :9
# 30 frames :7
# 31 frames :4
# 32 frames :6
# 33 frames :3
# 34 frames :8
# 35 frames :4
# 36 frames :3
# 37 frames :3
# 38 frames :1
# 40 frames :1
# 43 frames :1
# 44 frames :1
# 45 frames :2
# 48 frames :1
# 49 frames :1
# 50 frames :1
# 52 frames :2
# 62 frames :1
# 82 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1702
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0