Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.45.14
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bb8af2f1439fb880aa13b0d3f339652a",
"name": "M1_20170604_1704_dsRNA_G4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:45:37"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8883 (max 8884) in '8883'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.3033
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75929e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 22 mean_circ: 0.948346
Ran for 1550 frames Cell Events: 50 mean_circ: 0.940858
Ran for 3050 frames Cell Events: 92 mean_circ: 0.945109
Ran for 3550 frames Cell Events: 111 mean_circ: 0.944364
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Ran for 4550 frames Cell Events: 153 mean_circ: 0.946175
Ran for 5050 frames Cell Events: 171 mean_circ: 0.94693
Ran for 5550 frames Cell Events: 193 mean_circ: 0.94521
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Ran for 7550 frames Cell Events: 253 mean_circ: 0.944031
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Ran for 11050 frames Cell Events: 342 mean_circ: 0.945566
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Ran for 12550 frames Cell Events: 375 mean_circ: 0.945242
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Ran for 70050 frames Cell Events: 1926 mean_circ: 0.94652
Ran for 71050 frames Cell Events: 1960 mean_circ: 0.946457
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Ran for 73050 frames Cell Events: 2017 mean_circ: 0.946413
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Ran for 83550 frames Cell Events: 2301 mean_circ: 0.946434
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Ran for 86050 frames Cell Events: 2400 mean_circ: 0.946521
Ran for 86550 frames Cell Events: 2408 mean_circ: 0.946561
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Ran for 91050 frames Cell Events: 2557 mean_circ: 0.946773
Ran for 91550 frames Cell Events: 2570 mean_circ: 0.9467
Ran for 92550 frames Cell Events: 2600 mean_circ: 0.946633
Ran for 95550 frames Cell Events: 2701 mean_circ: 0.946363
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Ran for 96550 frames Cell Events: 2725 mean_circ: 0.946422
Ran for 97050 frames Cell Events: 2736 mean_circ: 0.946314
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Ran for 100050 frames Cell Events: 2817 mean_circ: 0.946276
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Ran for 101050 frames Cell Events: 2845 mean_circ: 0.946279
Ran for 102050 frames Cell Events: 2874 mean_circ: 0.946213
Ran for 103050 frames Cell Events: 2908 mean_circ: 0.946255
Ran for 103550 frames Cell Events: 2925 mean_circ: 0.946261
Ran for 104050 frames Cell Events: 2940 mean_circ: 0.946252
Ran for 104550 frames Cell Events: 2945 mean_circ: 0.946202
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Ran for 123050 frames Cell Events: 3438 mean_circ: 0.946362
Ran for 123550 frames Cell Events: 3452 mean_circ: 0.9464
Ran for 124050 frames Cell Events: 3471 mean_circ: 0.946407
Ran for 124550 frames Cell Events: 3488 mean_circ: 0.946408
Ran for 125550 frames Cell Events: 3527 mean_circ: 0.946433
Ran for 126050 frames Cell Events: 3531 mean_circ: 0.946438
Ran for 126550 frames Cell Events: 3549 mean_circ: 0.946447
Ran for 128050 frames Cell Events: 3599 mean_circ: 0.946478
Ran for 128550 frames Cell Events: 3610 mean_circ: 0.946477
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Ran for 131050 frames Cell Events: 3674 mean_circ: 0.946554
Ran for 132050 frames Cell Events: 3703 mean_circ: 0.946542
Ran for 132550 frames Cell Events: 3717 mean_circ: 0.946536
Ran for 133050 frames Cell Events: 3728 mean_circ: 0.946495
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Ran for 136050 frames Cell Events: 3805 mean_circ: 0.946675
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Ran for 138050 frames Cell Events: 3862 mean_circ: 0.946688
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Ran for 141550 frames Cell Events: 3961 mean_circ: 0.94667
Ran for 142550 frames Cell Events: 3994 mean_circ: 0.946657
Ran for 143050 frames Cell Events: 4005 mean_circ: 0.94671
Ran for 143550 frames Cell Events: 4028 mean_circ: 0.946693
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Ran for 147050 frames Cell Events: 4125 mean_circ: 0.946682
Ran for 147550 frames Cell Events: 4145 mean_circ: 0.946733
Ran for 148050 frames Cell Events: 4157 mean_circ: 0.94676
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Ran for 200050 frames Cell Events: 5637 mean_circ: 0.946592
Ran for 201050 frames Cell Events: 5669 mean_circ: 0.9466
Ran for 201550 frames Cell Events: 5696 mean_circ: 0.946626
Ran for 202550 frames Cell Events: 5727 mean_circ: 0.946645
Ran for 203550 frames Cell Events: 5756 mean_circ: 0.94664
Ran for 205050 frames Cell Events: 5818 mean_circ: 0.946714
Ran for 205550 frames Cell Events: 5828 mean_circ: 0.946769
Ran for 206050 frames Cell Events: 5842 mean_circ: 0.946768
Ran for 206550 frames Cell Events: 5854 mean_circ: 0.946713
Ran for 207050 frames Cell Events: 5865 mean_circ: 0.946734
Ran for 207550 frames Cell Events: 5873 mean_circ: 0.946745
Ran for 208050 frames Cell Events: 5883 mean_circ: 0.946763
Ran for 210050 frames Cell Events: 5939 mean_circ: 0.94681
Ran for 211550 frames Cell Events: 5995 mean_circ: 0.946774
Ran for 212550 frames Cell Events: 6038 mean_circ: 0.946829
Ran for 213050 frames Cell Events: 6044 mean_circ: 0.946862
Ran for 214550 frames Cell Events: 6090 mean_circ: 0.946886
Ran for 215050 frames Cell Events: 6102 mean_circ: 0.946916
Ran for 216050 frames Cell Events: 6127 mean_circ: 0.946958
Ran for 216550 frames Cell Events: 6139 mean_circ: 0.946977
Ran for 217050 frames Cell Events: 6149 mean_circ: 0.946979
Ran for 219550 frames Cell Events: 6233 mean_circ: 0.947026
Ran for 220050 frames Cell Events: 6248 mean_circ: 0.946994
Ran for 220550 frames Cell Events: 6258 mean_circ: 0.946942
Ran for 222050 frames Cell Events: 6299 mean_circ: 0.94694
Ran for 223050 frames Cell Events: 6338 mean_circ: 0.946938
Ran for 223550 frames Cell Events: 6349 mean_circ: 0.946923
Ran for 224050 frames Cell Events: 6361 mean_circ: 0.946902
Ran for 225050 frames Cell Events: 6399 mean_circ: 0.946878
Ran for 228050 frames Cell Events: 6501 mean_circ: 0.946865
Ran for 230550 frames Cell Events: 6578 mean_circ: 0.94695
Ran for 231050 frames Cell Events: 6586 mean_circ: 0.946901
Ran for 231550 frames Cell Events: 6601 mean_circ: 0.946895
Ran for 232050 frames Cell Events: 6611 mean_circ: 0.946911
Ran for 233050 frames Cell Events: 6630 mean_circ: 0.946948
Ran for 233550 frames Cell Events: 6644 mean_circ: 0.946957
Ran for 234550 frames Cell Events: 6667 mean_circ: 0.946988
Ran for 235050 frames Cell Events: 6683 mean_circ: 0.947009
Ran for 236550 frames Cell Events: 6739 mean_circ: 0.94698
Ran for 237550 frames Cell Events: 6773 mean_circ: 0.946983
Ran for 239050 frames Cell Events: 6822 mean_circ: 0.947027
Ran for 239550 frames Cell Events: 6836 mean_circ: 0.947027
Ran for 241550 frames Cell Events: 6896 mean_circ: 0.9469
Ran for 242050 frames Cell Events: 6922 mean_circ: 0.9469
Ran for 242550 frames Cell Events: 6936 mean_circ: 0.946956
Ran for 243050 frames Cell Events: 6950 mean_circ: 0.946975
Ran for 244050 frames Cell Events: 6968 mean_circ: 0.946999
Ran for 244550 frames Cell Events: 6982 mean_circ: 0.947
Ran for 245050 frames Cell Events: 6990 mean_circ: 0.947
Ran for 245550 frames Cell Events: 6999 mean_circ: 0.946991
Ran for 246550 frames Cell Events: 7026 mean_circ: 0.947018
Ran for 247050 frames Cell Events: 7039 mean_circ: 0.946994
Ran for 247550 frames Cell Events: 7052 mean_circ: 0.947004
Ran for 248050 frames Cell Events: 7065 mean_circ: 0.947004
Ran for 248550 frames Cell Events: 7078 mean_circ: 0.947022
Ran for 249050 frames Cell Events: 7097 mean_circ: 0.947032
Ran for 249550 frames Cell Events: 7113 mean_circ: 0.947028
Ran for 250050 frames Cell Events: 7126 mean_circ: 0.947047
Ran for 251050 frames Cell Events: 7161 mean_circ: 0.947032
Ran for 251550 frames Cell Events: 7173 mean_circ: 0.947061
Ran for 253050 frames Cell Events: 7209 mean_circ: 0.946993
Ran for 254050 frames Cell Events: 7236 mean_circ: 0.946965
Ran for 255050 frames Cell Events: 7258 mean_circ: 0.946927
Ran for 256050 frames Cell Events: 7289 mean_circ: 0.946924
Ran for 257050 frames Cell Events: 7321 mean_circ: 0.946891
Ran for 257550 frames Cell Events: 7333 mean_circ: 0.94692
Ran for 259050 frames Cell Events: 7389 mean_circ: 0.946962
Ran for 260050 frames Cell Events: 7411 mean_circ: 0.946977
Ran for 260550 frames Cell Events: 7419 mean_circ: 0.946995
Ran for 261050 frames Cell Events: 7437 mean_circ: 0.946995
Ran for 263050 frames Cell Events: 7511 mean_circ: 0.946966
Ran for 264050 frames Cell Events: 7534 mean_circ: 0.946955
Ran for 265550 frames Cell Events: 7574 mean_circ: 0.94696
Ran for 266050 frames Cell Events: 7587 mean_circ: 0.946961
Ran for 266550 frames Cell Events: 7601 mean_circ: 0.946977
Ran for 267050 frames Cell Events: 7624 mean_circ: 0.946992
Ran for 267550 frames Cell Events: 7645 mean_circ: 0.947005
Ran for 268550 frames Cell Events: 7691 mean_circ: 0.947022
Ran for 269050 frames Cell Events: 7704 mean_circ: 0.947034
Ran for 269550 frames Cell Events: 7716 mean_circ: 0.947014
Ran for 270050 frames Cell Events: 7735 mean_circ: 0.946997
Ran for 271050 frames Cell Events: 7762 mean_circ: 0.947009
Ran for 272550 frames Cell Events: 7802 mean_circ: 0.947016
Ran for 273550 frames Cell Events: 7841 mean_circ: 0.947078
Ran for 274050 frames Cell Events: 7851 mean_circ: 0.947068
Ran for 274550 frames Cell Events: 7867 mean_circ: 0.947058
Ran for 275050 frames Cell Events: 7884 mean_circ: 0.947017
Ran for 277050 frames Cell Events: 7960 mean_circ: 0.947037
Ran for 278550 frames Cell Events: 8004 mean_circ: 0.947052
Ran for 279050 frames Cell Events: 8017 mean_circ: 0.947054
Ran for 280050 frames Cell Events: 8064 mean_circ: 0.947055
Ran for 281050 frames Cell Events: 8091 mean_circ: 0.947049
Ran for 281550 frames Cell Events: 8099 mean_circ: 0.947044
Ran for 282550 frames Cell Events: 8129 mean_circ: 0.947026
Ran for 283050 frames Cell Events: 8139 mean_circ: 0.947007
Ran for 283550 frames Cell Events: 8158 mean_circ: 0.946993
Ran for 284050 frames Cell Events: 8179 mean_circ: 0.947042
Ran for 284550 frames Cell Events: 8193 mean_circ: 0.947043
Ran for 287050 frames Cell Events: 8260 mean_circ: 0.94704
Ran for 288050 frames Cell Events: 8277 mean_circ: 0.947062
Ran for 289050 frames Cell Events: 8302 mean_circ: 0.947061
Ran for 289550 frames Cell Events: 8305 mean_circ: 0.947065
Ran for 290050 frames Cell Events: 8317 mean_circ: 0.947063
Ran for 290550 frames Cell Events: 8328 mean_circ: 0.947061
Ran for 291050 frames Cell Events: 8335 mean_circ: 0.947077
Ran for 291550 frames Cell Events: 8344 mean_circ: 0.947068
Ran for 292550 frames Cell Events: 8376 mean_circ: 0.947085
Ran for 293050 frames Cell Events: 8396 mean_circ: 0.947104
Ran for 295050 frames Cell Events: 8460 mean_circ: 0.947033
Ran for 295550 frames Cell Events: 8479 mean_circ: 0.947048
Ran for 297050 frames Cell Events: 8524 mean_circ: 0.947072
Ran for 297550 frames Cell Events: 8540 mean_circ: 0.947078
Ran for 298550 frames Cell Events: 8578 mean_circ: 0.947089
Ran for 299050 frames Cell Events: 8594 mean_circ: 0.947079
Ran for 299550 frames Cell Events: 8602 mean_circ: 0.947051
Ran for 300050 frames Cell Events: 8614 mean_circ: 0.947039
Ran for 301050 frames Cell Events: 8642 mean_circ: 0.947061
Ran for 301550 frames Cell Events: 8661 mean_circ: 0.947067
Ran for 303050 frames Cell Events: 8694 mean_circ: 0.947063
Ran for 304550 frames Cell Events: 8754 mean_circ: 0.947013
Ran for 305050 frames Cell Events: 8770 mean_circ: 0.94702
Ran for 306550 frames Cell Events: 8823 mean_circ: 0.94705
End this measurment..
Stopping time: 1.75944e+009
****************************************
Measurement finished
Mean Circularitiy: 0.946984
Mean Area: 609.87
Mean FL-1 intensity: 3435.09
Mean FL-2 intensity: 3993.98
Mean FL-3 intensity: 0
frames processed: 307454
Total Time: 154330ms
Number of Runs (new bg img) 0
Frames collected: 308528
Skipped frames: 88674
Rescued Frames: 2974531
Samples DAQ: 77134180
Samples written: 154264154
Samples per Channel: 77132077
FPS: 1999.15
Samples per second: 499787
Expected samples/s: 500000
Events: 8884
Events per second: 57.565
jumped back (PMT): 4355
#Frames read, frequency
# 0 frames :451007
# 1 frames :144742
# 2 frames :4925
# 3 frames :2724
# 4 frames :1386
# 5 frames :929
# 6 frames :900
# 7 frames :1667
# 8 frames :1319
# 9 frames :1031
# 10 frames :998
# 11 frames :917
# 12 frames :800
# 13 frames :602
# 14 frames :399
# 15 frames :298
# 16 frames :281
# 17 frames :245
# 18 frames :222
# 19 frames :214
# 20 frames :204
# 21 frames :218
# 22 frames :233
# 23 frames :166
# 24 frames :95
# 25 frames :69
# 26 frames :50
# 27 frames :46
# 28 frames :37
# 29 frames :34
# 30 frames :30
# 31 frames :25
# 32 frames :22
# 33 frames :20
# 34 frames :17
# 35 frames :17
# 36 frames :9
# 37 frames :10
# 38 frames :12
# 39 frames :4
# 40 frames :6
# 41 frames :2
# 42 frames :2
# 43 frames :4
# 44 frames :6
# 45 frames :4
# 46 frames :2
# 47 frames :5
# 48 frames :4
# 49 frames :1
# 50 frames :1
# 52 frames :2
# 53 frames :1
# 54 frames :1
# 59 frames :1
# 60 frames :1
# 61 frames :1
# 63 frames :1
# 67 frames :2
# 68 frames :1
# 69 frames :1
# 70 frames :1
# 84 frames :1
# 94 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.3033
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0