Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.49.20
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fc85ebaad7fe86ac9e5835122450efcb",
"name": "M1_20170604_1755_dsRNA_LZ_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:49:40"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8144 (max 8145) in '8144'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2424
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.76237e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 35 mean_circ: 0.938005
Ran for 2050 frames Cell Events: 76 mean_circ: 0.933999
Ran for 2550 frames Cell Events: 90 mean_circ: 0.933525
Ran for 3050 frames Cell Events: 110 mean_circ: 0.932086
Ran for 4050 frames Cell Events: 141 mean_circ: 0.931848
Ran for 4550 frames Cell Events: 160 mean_circ: 0.934178
Ran for 5050 frames Cell Events: 187 mean_circ: 0.935561
Ran for 6550 frames Cell Events: 239 mean_circ: 0.935879
Ran for 7050 frames Cell Events: 256 mean_circ: 0.935131
Ran for 8050 frames Cell Events: 298 mean_circ: 0.934977
Ran for 8550 frames Cell Events: 316 mean_circ: 0.9346
Ran for 9050 frames Cell Events: 333 mean_circ: 0.935275
Ran for 9550 frames Cell Events: 358 mean_circ: 0.934807
Ran for 10050 frames Cell Events: 368 mean_circ: 0.935069
Ran for 10550 frames Cell Events: 386 mean_circ: 0.934818
Ran for 11050 frames Cell Events: 401 mean_circ: 0.934677
Ran for 12550 frames Cell Events: 452 mean_circ: 0.935093
Ran for 13050 frames Cell Events: 470 mean_circ: 0.935299
Ran for 13550 frames Cell Events: 487 mean_circ: 0.935492
Ran for 16050 frames Cell Events: 589 mean_circ: 0.935334
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Ran for 35550 frames Cell Events: 1274 mean_circ: 0.934532
Ran for 36050 frames Cell Events: 1303 mean_circ: 0.93429
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Ran for 53050 frames Cell Events: 1935 mean_circ: 0.933789
Ran for 53550 frames Cell Events: 1953 mean_circ: 0.933834
Ran for 54050 frames Cell Events: 1969 mean_circ: 0.933745
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Ran for 62550 frames Cell Events: 2303 mean_circ: 0.933753
Ran for 63550 frames Cell Events: 2349 mean_circ: 0.933863
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Ran for 67050 frames Cell Events: 2506 mean_circ: 0.933827
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Ran for 69050 frames Cell Events: 2612 mean_circ: 0.933944
Ran for 70550 frames Cell Events: 2667 mean_circ: 0.934097
Ran for 71050 frames Cell Events: 2688 mean_circ: 0.934201
Ran for 71550 frames Cell Events: 2706 mean_circ: 0.934229
Ran for 72050 frames Cell Events: 2726 mean_circ: 0.934255
Ran for 72550 frames Cell Events: 2744 mean_circ: 0.934087
Ran for 73050 frames Cell Events: 2768 mean_circ: 0.934067
Ran for 73550 frames Cell Events: 2792 mean_circ: 0.934158
Ran for 74550 frames Cell Events: 2824 mean_circ: 0.934311
Ran for 75050 frames Cell Events: 2839 mean_circ: 0.934224
Ran for 76050 frames Cell Events: 2887 mean_circ: 0.934289
Ran for 76550 frames Cell Events: 2906 mean_circ: 0.934438
Ran for 77550 frames Cell Events: 2955 mean_circ: 0.934541
Ran for 78050 frames Cell Events: 2977 mean_circ: 0.934477
Ran for 78550 frames Cell Events: 2991 mean_circ: 0.934447
Ran for 79550 frames Cell Events: 3033 mean_circ: 0.934555
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Ran for 80550 frames Cell Events: 3073 mean_circ: 0.934523
Ran for 81550 frames Cell Events: 3117 mean_circ: 0.934497
Ran for 82050 frames Cell Events: 3134 mean_circ: 0.934395
Ran for 83050 frames Cell Events: 3179 mean_circ: 0.934377
Ran for 84050 frames Cell Events: 3212 mean_circ: 0.934363
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Ran for 85550 frames Cell Events: 3262 mean_circ: 0.934432
Ran for 86050 frames Cell Events: 3271 mean_circ: 0.934429
Ran for 86550 frames Cell Events: 3286 mean_circ: 0.934492
Ran for 87050 frames Cell Events: 3300 mean_circ: 0.934602
Ran for 87550 frames Cell Events: 3319 mean_circ: 0.934622
Ran for 88050 frames Cell Events: 3341 mean_circ: 0.934641
Ran for 88550 frames Cell Events: 3358 mean_circ: 0.93459
Ran for 89050 frames Cell Events: 3376 mean_circ: 0.934545
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Ran for 90050 frames Cell Events: 3410 mean_circ: 0.934658
Ran for 90550 frames Cell Events: 3432 mean_circ: 0.934712
Ran for 92550 frames Cell Events: 3516 mean_circ: 0.934782
Ran for 93050 frames Cell Events: 3532 mean_circ: 0.934806
Ran for 93550 frames Cell Events: 3551 mean_circ: 0.934816
Ran for 94550 frames Cell Events: 3577 mean_circ: 0.934857
Ran for 95050 frames Cell Events: 3600 mean_circ: 0.934853
Ran for 95550 frames Cell Events: 3614 mean_circ: 0.93484
Ran for 96050 frames Cell Events: 3632 mean_circ: 0.934835
Ran for 96550 frames Cell Events: 3653 mean_circ: 0.934842
Ran for 97050 frames Cell Events: 3670 mean_circ: 0.934788
Ran for 99050 frames Cell Events: 3759 mean_circ: 0.934629
Ran for 99550 frames Cell Events: 3776 mean_circ: 0.93465
Ran for 101050 frames Cell Events: 3842 mean_circ: 0.934663
Ran for 102050 frames Cell Events: 3885 mean_circ: 0.934702
Ran for 102550 frames Cell Events: 3898 mean_circ: 0.934711
Ran for 103050 frames Cell Events: 3919 mean_circ: 0.934646
Ran for 103550 frames Cell Events: 3933 mean_circ: 0.934689
Ran for 104050 frames Cell Events: 3947 mean_circ: 0.934678
Ran for 105050 frames Cell Events: 3981 mean_circ: 0.934608
Ran for 106050 frames Cell Events: 4023 mean_circ: 0.934654
Ran for 107050 frames Cell Events: 4073 mean_circ: 0.934683
Ran for 107550 frames Cell Events: 4086 mean_circ: 0.934681
Ran for 108050 frames Cell Events: 4101 mean_circ: 0.934671
Ran for 108550 frames Cell Events: 4119 mean_circ: 0.934649
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Ran for 118050 frames Cell Events: 4490 mean_circ: 0.934702
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Ran for 120550 frames Cell Events: 4602 mean_circ: 0.934534
Ran for 121550 frames Cell Events: 4641 mean_circ: 0.934523
Ran for 122050 frames Cell Events: 4655 mean_circ: 0.934545
Ran for 122550 frames Cell Events: 4662 mean_circ: 0.934564
Ran for 124050 frames Cell Events: 4721 mean_circ: 0.934537
Ran for 124550 frames Cell Events: 4745 mean_circ: 0.934501
Ran for 126550 frames Cell Events: 4832 mean_circ: 0.934438
Ran for 127050 frames Cell Events: 4845 mean_circ: 0.934485
Ran for 127550 frames Cell Events: 4871 mean_circ: 0.934414
Ran for 128050 frames Cell Events: 4889 mean_circ: 0.934382
Ran for 129050 frames Cell Events: 4932 mean_circ: 0.934356
Ran for 129550 frames Cell Events: 4950 mean_circ: 0.934326
Ran for 130050 frames Cell Events: 4964 mean_circ: 0.934306
Ran for 130550 frames Cell Events: 4991 mean_circ: 0.934299
Ran for 131550 frames Cell Events: 5022 mean_circ: 0.934265
Ran for 132050 frames Cell Events: 5039 mean_circ: 0.934272
Ran for 133050 frames Cell Events: 5088 mean_circ: 0.934325
Ran for 133550 frames Cell Events: 5106 mean_circ: 0.934316
Ran for 134550 frames Cell Events: 5144 mean_circ: 0.934312
Ran for 135550 frames Cell Events: 5189 mean_circ: 0.934258
Ran for 136050 frames Cell Events: 5218 mean_circ: 0.93431
Ran for 136550 frames Cell Events: 5238 mean_circ: 0.934329
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Ran for 139050 frames Cell Events: 5335 mean_circ: 0.934431
Ran for 139550 frames Cell Events: 5352 mean_circ: 0.934442
Ran for 140050 frames Cell Events: 5370 mean_circ: 0.934423
Ran for 141550 frames Cell Events: 5435 mean_circ: 0.934409
Ran for 142050 frames Cell Events: 5447 mean_circ: 0.934283
Ran for 143050 frames Cell Events: 5486 mean_circ: 0.934338
Ran for 143550 frames Cell Events: 5505 mean_circ: 0.934353
Ran for 144050 frames Cell Events: 5514 mean_circ: 0.934359
Ran for 144550 frames Cell Events: 5528 mean_circ: 0.934336
Ran for 145050 frames Cell Events: 5553 mean_circ: 0.934331
Ran for 146050 frames Cell Events: 5585 mean_circ: 0.934364
Ran for 146550 frames Cell Events: 5618 mean_circ: 0.934257
Ran for 147550 frames Cell Events: 5669 mean_circ: 0.934331
Ran for 149050 frames Cell Events: 5728 mean_circ: 0.934399
Ran for 150050 frames Cell Events: 5754 mean_circ: 0.934409
Ran for 150550 frames Cell Events: 5775 mean_circ: 0.934407
Ran for 151050 frames Cell Events: 5793 mean_circ: 0.934397
Ran for 151550 frames Cell Events: 5813 mean_circ: 0.934394
Ran for 152050 frames Cell Events: 5839 mean_circ: 0.934406
Ran for 152550 frames Cell Events: 5858 mean_circ: 0.934421
Ran for 153050 frames Cell Events: 5876 mean_circ: 0.93448
Ran for 154050 frames Cell Events: 5919 mean_circ: 0.934468
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Ran for 156550 frames Cell Events: 6002 mean_circ: 0.934326
Ran for 157050 frames Cell Events: 6021 mean_circ: 0.934311
Ran for 158050 frames Cell Events: 6059 mean_circ: 0.934308
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Ran for 171550 frames Cell Events: 6567 mean_circ: 0.93437
Ran for 172550 frames Cell Events: 6609 mean_circ: 0.934425
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Ran for 173550 frames Cell Events: 6636 mean_circ: 0.934418
Ran for 174050 frames Cell Events: 6665 mean_circ: 0.934422
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Ran for 177050 frames Cell Events: 6793 mean_circ: 0.934405
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Ran for 201550 frames Cell Events: 7802 mean_circ: 0.934582
Ran for 202050 frames Cell Events: 7821 mean_circ: 0.93459
Ran for 203050 frames Cell Events: 7857 mean_circ: 0.934589
Ran for 204550 frames Cell Events: 7918 mean_circ: 0.934634
Ran for 205050 frames Cell Events: 7942 mean_circ: 0.934611
Ran for 205550 frames Cell Events: 7956 mean_circ: 0.934628
Ran for 206050 frames Cell Events: 7973 mean_circ: 0.934617
Ran for 207550 frames Cell Events: 8032 mean_circ: 0.934559
Ran for 208050 frames Cell Events: 8049 mean_circ: 0.934566
Ran for 208550 frames Cell Events: 8060 mean_circ: 0.934556
Ran for 209550 frames Cell Events: 8105 mean_circ: 0.934483
Ran for 210050 frames Cell Events: 8120 mean_circ: 0.934484
End this measurment..
Stopping time: 1.76247e+009
****************************************
Measurement finished
Mean Circularitiy: 0.93446
Mean Area: 626.819
Mean FL-1 intensity: 3260.96
Mean FL-2 intensity: 4431.33
Mean FL-3 intensity: 0
frames processed: 209516
Total Time: 105376ms
Number of Runs (new bg img) 0
Frames collected: 210635
Skipped frames: 108517
Rescued Frames: 3895024
Samples DAQ: 52658891
Samples written: 105317686
Samples per Channel: 52658843
FPS: 1998.9
Samples per second: 499725
Expected samples/s: 500000
Events: 8145
Events per second: 77.2949
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :298455
# 1 frames :106142
# 2 frames :4893
# 3 frames :1597
# 4 frames :820
# 5 frames :328
# 6 frames :2052
# 7 frames :1738
# 8 frames :1084
# 9 frames :662
# 10 frames :935
# 11 frames :846
# 12 frames :463
# 13 frames :212
# 14 frames :157
# 15 frames :160
# 16 frames :154
# 17 frames :122
# 18 frames :66
# 19 frames :54
# 20 frames :59
# 21 frames :46
# 22 frames :39
# 23 frames :28
# 24 frames :23
# 25 frames :26
# 26 frames :3
# 27 frames :12
# 28 frames :10
# 29 frames :11
# 30 frames :3
# 31 frames :3
# 32 frames :3
# 33 frames :2
# 34 frames :1
# 35 frames :3
# 36 frames :1
# 38 frames :1
# 42 frames :1
# 65 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2424
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0