Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.55.32
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ac190817b17a88afd88d2e53f4b09a73",
"name": "M1_20170604_1923_dsRNA_G8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:55:55"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9817 (max 9818) in '9817'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1928
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.76762e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 39 mean_circ: 0.942176
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Ran for 72550 frames Cell Events: 2750 mean_circ: 0.942935
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Ran for 83550 frames Cell Events: 3159 mean_circ: 0.943234
Ran for 84050 frames Cell Events: 3181 mean_circ: 0.943226
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Ran for 92050 frames Cell Events: 3447 mean_circ: 0.943398
Ran for 92550 frames Cell Events: 3472 mean_circ: 0.94334
Ran for 93050 frames Cell Events: 3492 mean_circ: 0.943384
Ran for 94050 frames Cell Events: 3526 mean_circ: 0.943438
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Ran for 96550 frames Cell Events: 3609 mean_circ: 0.943521
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Ran for 100550 frames Cell Events: 3767 mean_circ: 0.943309
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Ran for 102550 frames Cell Events: 3833 mean_circ: 0.943329
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Ran for 103550 frames Cell Events: 3861 mean_circ: 0.94325
Ran for 104050 frames Cell Events: 3885 mean_circ: 0.943272
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Ran for 121550 frames Cell Events: 4462 mean_circ: 0.943251
Ran for 122050 frames Cell Events: 4491 mean_circ: 0.943232
Ran for 123050 frames Cell Events: 4536 mean_circ: 0.943277
Ran for 123550 frames Cell Events: 4554 mean_circ: 0.943298
Ran for 124550 frames Cell Events: 4594 mean_circ: 0.943264
Ran for 125050 frames Cell Events: 4621 mean_circ: 0.943275
Ran for 125550 frames Cell Events: 4637 mean_circ: 0.94334
Ran for 126550 frames Cell Events: 4676 mean_circ: 0.943301
Ran for 127550 frames Cell Events: 4715 mean_circ: 0.943332
Ran for 128050 frames Cell Events: 4729 mean_circ: 0.943405
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Ran for 130050 frames Cell Events: 4819 mean_circ: 0.943505
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Ran for 131050 frames Cell Events: 4872 mean_circ: 0.943558
Ran for 132550 frames Cell Events: 4948 mean_circ: 0.943533
Ran for 133050 frames Cell Events: 4976 mean_circ: 0.943499
Ran for 134050 frames Cell Events: 5015 mean_circ: 0.943522
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Ran for 141550 frames Cell Events: 5332 mean_circ: 0.943502
Ran for 142050 frames Cell Events: 5343 mean_circ: 0.943517
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Ran for 172050 frames Cell Events: 6416 mean_circ: 0.944052
Ran for 172550 frames Cell Events: 6426 mean_circ: 0.944072
Ran for 173550 frames Cell Events: 6449 mean_circ: 0.944035
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Ran for 182550 frames Cell Events: 6812 mean_circ: 0.944107
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Ran for 185550 frames Cell Events: 6931 mean_circ: 0.944072
Ran for 186050 frames Cell Events: 6951 mean_circ: 0.944126
Ran for 186550 frames Cell Events: 6976 mean_circ: 0.944117
Ran for 187550 frames Cell Events: 7009 mean_circ: 0.944111
Ran for 188050 frames Cell Events: 7024 mean_circ: 0.944124
Ran for 188550 frames Cell Events: 7037 mean_circ: 0.944102
Ran for 189050 frames Cell Events: 7059 mean_circ: 0.94411
Ran for 189550 frames Cell Events: 7077 mean_circ: 0.944112
Ran for 190050 frames Cell Events: 7095 mean_circ: 0.944128
Ran for 190550 frames Cell Events: 7108 mean_circ: 0.944143
Ran for 191050 frames Cell Events: 7129 mean_circ: 0.944131
Ran for 192050 frames Cell Events: 7182 mean_circ: 0.944088
Ran for 192550 frames Cell Events: 7201 mean_circ: 0.944068
Ran for 193050 frames Cell Events: 7213 mean_circ: 0.944068
Ran for 193550 frames Cell Events: 7232 mean_circ: 0.944089
Ran for 194050 frames Cell Events: 7250 mean_circ: 0.944067
Ran for 195050 frames Cell Events: 7287 mean_circ: 0.944127
Ran for 195550 frames Cell Events: 7310 mean_circ: 0.944136
Ran for 196050 frames Cell Events: 7346 mean_circ: 0.944096
Ran for 196550 frames Cell Events: 7359 mean_circ: 0.944058
Ran for 197050 frames Cell Events: 7384 mean_circ: 0.943979
Ran for 198050 frames Cell Events: 7427 mean_circ: 0.943993
Ran for 198550 frames Cell Events: 7448 mean_circ: 0.943994
Ran for 199050 frames Cell Events: 7471 mean_circ: 0.943958
Ran for 199550 frames Cell Events: 7501 mean_circ: 0.943942
Ran for 200550 frames Cell Events: 7530 mean_circ: 0.943904
Ran for 201050 frames Cell Events: 7548 mean_circ: 0.943895
Ran for 202050 frames Cell Events: 7583 mean_circ: 0.943906
Ran for 202550 frames Cell Events: 7606 mean_circ: 0.943933
Ran for 203050 frames Cell Events: 7622 mean_circ: 0.943927
Ran for 204050 frames Cell Events: 7646 mean_circ: 0.943971
Ran for 204550 frames Cell Events: 7668 mean_circ: 0.943974
Ran for 205050 frames Cell Events: 7690 mean_circ: 0.944039
Ran for 205550 frames Cell Events: 7713 mean_circ: 0.944082
Ran for 206050 frames Cell Events: 7735 mean_circ: 0.944082
Ran for 206550 frames Cell Events: 7751 mean_circ: 0.944061
Ran for 207550 frames Cell Events: 7795 mean_circ: 0.944092
Ran for 208550 frames Cell Events: 7845 mean_circ: 0.944091
Ran for 209050 frames Cell Events: 7868 mean_circ: 0.944127
Ran for 210050 frames Cell Events: 7919 mean_circ: 0.944102
Ran for 211050 frames Cell Events: 7964 mean_circ: 0.944128
Ran for 211550 frames Cell Events: 7982 mean_circ: 0.944114
Ran for 212550 frames Cell Events: 8032 mean_circ: 0.944126
Ran for 213050 frames Cell Events: 8051 mean_circ: 0.944144
Ran for 213550 frames Cell Events: 8066 mean_circ: 0.944173
Ran for 214050 frames Cell Events: 8090 mean_circ: 0.944183
Ran for 215050 frames Cell Events: 8132 mean_circ: 0.944158
Ran for 215550 frames Cell Events: 8151 mean_circ: 0.944152
Ran for 216550 frames Cell Events: 8188 mean_circ: 0.944179
Ran for 217050 frames Cell Events: 8211 mean_circ: 0.944188
Ran for 217550 frames Cell Events: 8228 mean_circ: 0.944215
Ran for 218050 frames Cell Events: 8247 mean_circ: 0.944237
Ran for 219550 frames Cell Events: 8333 mean_circ: 0.944208
Ran for 220050 frames Cell Events: 8352 mean_circ: 0.944111
Ran for 220550 frames Cell Events: 8376 mean_circ: 0.944107
Ran for 223550 frames Cell Events: 8525 mean_circ: 0.944039
Ran for 224050 frames Cell Events: 8544 mean_circ: 0.944011
Ran for 225550 frames Cell Events: 8619 mean_circ: 0.943975
Ran for 226050 frames Cell Events: 8645 mean_circ: 0.944015
Ran for 226550 frames Cell Events: 8663 mean_circ: 0.944006
Ran for 227050 frames Cell Events: 8698 mean_circ: 0.94404
Ran for 228050 frames Cell Events: 8739 mean_circ: 0.944056
Ran for 228550 frames Cell Events: 8759 mean_circ: 0.944065
Ran for 229050 frames Cell Events: 8775 mean_circ: 0.944073
Ran for 229550 frames Cell Events: 8792 mean_circ: 0.944064
Ran for 230550 frames Cell Events: 8820 mean_circ: 0.944066
Ran for 231050 frames Cell Events: 8840 mean_circ: 0.94407
Ran for 231550 frames Cell Events: 8857 mean_circ: 0.944093
Ran for 232050 frames Cell Events: 8867 mean_circ: 0.944126
Ran for 232550 frames Cell Events: 8889 mean_circ: 0.94412
Ran for 235050 frames Cell Events: 8995 mean_circ: 0.944101
Ran for 236050 frames Cell Events: 9026 mean_circ: 0.944115
Ran for 236550 frames Cell Events: 9046 mean_circ: 0.944138
Ran for 237050 frames Cell Events: 9061 mean_circ: 0.944161
Ran for 237550 frames Cell Events: 9073 mean_circ: 0.944183
Ran for 238050 frames Cell Events: 9083 mean_circ: 0.944188
Ran for 238550 frames Cell Events: 9101 mean_circ: 0.944183
Ran for 239050 frames Cell Events: 9116 mean_circ: 0.944185
Ran for 239550 frames Cell Events: 9138 mean_circ: 0.94418
Ran for 240050 frames Cell Events: 9150 mean_circ: 0.944189
Ran for 240550 frames Cell Events: 9176 mean_circ: 0.944164
Ran for 241050 frames Cell Events: 9193 mean_circ: 0.94417
Ran for 241550 frames Cell Events: 9208 mean_circ: 0.944134
Ran for 242050 frames Cell Events: 9222 mean_circ: 0.94411
Ran for 242550 frames Cell Events: 9240 mean_circ: 0.94413
Ran for 243050 frames Cell Events: 9263 mean_circ: 0.944129
Ran for 244050 frames Cell Events: 9293 mean_circ: 0.94415
Ran for 245550 frames Cell Events: 9347 mean_circ: 0.94415
Ran for 247050 frames Cell Events: 9412 mean_circ: 0.944067
Ran for 247550 frames Cell Events: 9440 mean_circ: 0.944078
Ran for 249550 frames Cell Events: 9512 mean_circ: 0.944026
Ran for 250050 frames Cell Events: 9530 mean_circ: 0.944041
Ran for 250550 frames Cell Events: 9547 mean_circ: 0.944067
Ran for 251050 frames Cell Events: 9559 mean_circ: 0.944076
Ran for 251550 frames Cell Events: 9578 mean_circ: 0.944092
Ran for 252050 frames Cell Events: 9593 mean_circ: 0.944095
Ran for 253050 frames Cell Events: 9632 mean_circ: 0.94408
Ran for 254050 frames Cell Events: 9671 mean_circ: 0.944097
Ran for 254550 frames Cell Events: 9683 mean_circ: 0.944096
Ran for 256550 frames Cell Events: 9748 mean_circ: 0.944075
Ran for 257550 frames Cell Events: 9769 mean_circ: 0.944094
Ran for 258050 frames Cell Events: 9786 mean_circ: 0.944085
Ran for 258550 frames Cell Events: 9808 mean_circ: 0.944077
End this measurment..
Stopping time: 1.76775e+009
****************************************
Measurement finished
Mean Circularitiy: 0.944094
Mean Area: 620.258
Mean FL-1 intensity: 3541.52
Mean FL-2 intensity: 3986.2
Mean FL-3 intensity: 0
frames processed: 256747
Total Time: 129538ms
Number of Runs (new bg img) 0
Frames collected: 258936
Skipped frames: 147694
Rescued Frames: 5261844
Samples DAQ: 64737931
Samples written: 129468000
Samples per Channel: 64734000
FPS: 1998.92
Samples per second: 499729
Expected samples/s: 500000
Events: 9818
Events per second: 75.7923
jumped back (PMT): 6904
#Frames read, frequency
# 0 frames :353868
# 1 frames :144771
# 2 frames :6107
# 3 frames :1490
# 4 frames :770
# 5 frames :272
# 6 frames :3598
# 7 frames :2393
# 8 frames :1469
# 9 frames :645
# 10 frames :500
# 11 frames :290
# 12 frames :340
# 13 frames :237
# 14 frames :286
# 15 frames :155
# 16 frames :139
# 17 frames :75
# 18 frames :65
# 19 frames :48
# 20 frames :57
# 21 frames :46
# 22 frames :32
# 23 frames :35
# 24 frames :23
# 25 frames :23
# 26 frames :13
# 27 frames :11
# 28 frames :12
# 29 frames :9
# 30 frames :9
# 31 frames :10
# 32 frames :4
# 33 frames :5
# 34 frames :2
# 35 frames :2
# 36 frames :1
# 37 frames :2
# 38 frames :1
# 39 frames :1
# 40 frames :2
# 41 frames :1
# 42 frames :2
# 43 frames :3
# 44 frames :1
# 47 frames :1
# 48 frames :1
# 50 frames :1
# 51 frames :1
# 58 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1928
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0