Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.58.14
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "273205f3a4c782f6885ab427a3a09567",
"name": "M1_20170604_1956_dsRNA_H8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:58:34"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8039 (max 8040) in '8039'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1751
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.76961e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 2 mean_circ: 0.935087
Ran for 550 frames Cell Events: 20 mean_circ: 0.946657
Ran for 1550 frames Cell Events: 58 mean_circ: 0.954009
Ran for 2050 frames Cell Events: 69 mean_circ: 0.953108
Ran for 4050 frames Cell Events: 137 mean_circ: 0.952868
Ran for 4550 frames Cell Events: 148 mean_circ: 0.951731
Ran for 5550 frames Cell Events: 176 mean_circ: 0.951855
Ran for 6550 frames Cell Events: 226 mean_circ: 0.952611
Ran for 7050 frames Cell Events: 241 mean_circ: 0.95216
Ran for 7550 frames Cell Events: 259 mean_circ: 0.952131
Ran for 8550 frames Cell Events: 296 mean_circ: 0.951618
Ran for 9550 frames Cell Events: 328 mean_circ: 0.951409
Ran for 10050 frames Cell Events: 335 mean_circ: 0.951485
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Ran for 13050 frames Cell Events: 436 mean_circ: 0.95101
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Ran for 15050 frames Cell Events: 494 mean_circ: 0.950416
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Ran for 76050 frames Cell Events: 2427 mean_circ: 0.949548
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Ran for 84050 frames Cell Events: 2672 mean_circ: 0.949233
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Ran for 92550 frames Cell Events: 2996 mean_circ: 0.949038
Ran for 93050 frames Cell Events: 3017 mean_circ: 0.949066
Ran for 93550 frames Cell Events: 3027 mean_circ: 0.949106
Ran for 94050 frames Cell Events: 3052 mean_circ: 0.94908
Ran for 95550 frames Cell Events: 3116 mean_circ: 0.949136
Ran for 96050 frames Cell Events: 3139 mean_circ: 0.949136
Ran for 96550 frames Cell Events: 3164 mean_circ: 0.949073
Ran for 97050 frames Cell Events: 3180 mean_circ: 0.949123
Ran for 97550 frames Cell Events: 3205 mean_circ: 0.949111
Ran for 98050 frames Cell Events: 3225 mean_circ: 0.949186
Ran for 98550 frames Cell Events: 3244 mean_circ: 0.949179
Ran for 99050 frames Cell Events: 3251 mean_circ: 0.949171
Ran for 100050 frames Cell Events: 3281 mean_circ: 0.949086
Ran for 100550 frames Cell Events: 3297 mean_circ: 0.949116
Ran for 101050 frames Cell Events: 3325 mean_circ: 0.948951
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Ran for 103050 frames Cell Events: 3396 mean_circ: 0.948996
Ran for 105550 frames Cell Events: 3494 mean_circ: 0.948839
Ran for 106050 frames Cell Events: 3511 mean_circ: 0.948794
Ran for 106550 frames Cell Events: 3538 mean_circ: 0.948784
Ran for 108050 frames Cell Events: 3593 mean_circ: 0.948741
Ran for 109550 frames Cell Events: 3661 mean_circ: 0.948707
Ran for 110550 frames Cell Events: 3687 mean_circ: 0.948694
Ran for 111050 frames Cell Events: 3703 mean_circ: 0.948606
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Ran for 125050 frames Cell Events: 4200 mean_circ: 0.9484
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Ran for 126050 frames Cell Events: 4235 mean_circ: 0.948409
Ran for 127050 frames Cell Events: 4273 mean_circ: 0.948429
Ran for 127550 frames Cell Events: 4284 mean_circ: 0.948443
Ran for 128050 frames Cell Events: 4304 mean_circ: 0.948422
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Ran for 129550 frames Cell Events: 4340 mean_circ: 0.948423
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Ran for 134050 frames Cell Events: 4488 mean_circ: 0.948463
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Ran for 143050 frames Cell Events: 4800 mean_circ: 0.948521
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Ran for 146050 frames Cell Events: 4918 mean_circ: 0.948537
Ran for 147550 frames Cell Events: 4983 mean_circ: 0.948568
Ran for 150050 frames Cell Events: 5094 mean_circ: 0.948456
Ran for 152050 frames Cell Events: 5181 mean_circ: 0.948432
Ran for 152550 frames Cell Events: 5186 mean_circ: 0.948454
Ran for 153050 frames Cell Events: 5200 mean_circ: 0.948463
Ran for 153550 frames Cell Events: 5209 mean_circ: 0.948456
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Ran for 156550 frames Cell Events: 5310 mean_circ: 0.948401
Ran for 157050 frames Cell Events: 5329 mean_circ: 0.948454
Ran for 157550 frames Cell Events: 5341 mean_circ: 0.948486
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Ran for 216550 frames Cell Events: 7407 mean_circ: 0.948971
Ran for 217050 frames Cell Events: 7420 mean_circ: 0.948986
Ran for 217550 frames Cell Events: 7436 mean_circ: 0.948988
Ran for 219050 frames Cell Events: 7480 mean_circ: 0.948982
Ran for 219550 frames Cell Events: 7492 mean_circ: 0.948999
Ran for 220050 frames Cell Events: 7508 mean_circ: 0.948992
Ran for 220550 frames Cell Events: 7524 mean_circ: 0.948999
Ran for 222050 frames Cell Events: 7586 mean_circ: 0.94895
Ran for 222550 frames Cell Events: 7604 mean_circ: 0.948969
Ran for 223050 frames Cell Events: 7614 mean_circ: 0.948976
Ran for 223550 frames Cell Events: 7630 mean_circ: 0.948941
Ran for 224550 frames Cell Events: 7661 mean_circ: 0.948989
Ran for 225050 frames Cell Events: 7677 mean_circ: 0.949005
Ran for 226050 frames Cell Events: 7713 mean_circ: 0.949013
Ran for 227050 frames Cell Events: 7745 mean_circ: 0.949044
Ran for 227550 frames Cell Events: 7757 mean_circ: 0.949066
Ran for 228050 frames Cell Events: 7773 mean_circ: 0.949093
Ran for 229050 frames Cell Events: 7812 mean_circ: 0.949091
Ran for 229550 frames Cell Events: 7833 mean_circ: 0.949109
Ran for 230050 frames Cell Events: 7862 mean_circ: 0.94908
Ran for 231550 frames Cell Events: 7911 mean_circ: 0.949097
Ran for 232550 frames Cell Events: 7947 mean_circ: 0.949088
Ran for 233550 frames Cell Events: 7995 mean_circ: 0.949112
Ran for 234050 frames Cell Events: 8020 mean_circ: 0.949146
End this measurment..
Stopping time: 1.76972e+009
****************************************
Measurement finished
Mean Circularitiy: 0.949152
Mean Area: 550.712
Mean FL-1 intensity: 3105.66
Mean FL-2 intensity: 3753.24
Mean FL-3 intensity: 0
frames processed: 232821
Total Time: 117455ms
Number of Runs (new bg img) 0
Frames collected: 234786
Skipped frames: 160708
Rescued Frames: 5777830
Samples DAQ: 58696487
Samples written: 117392742
Samples per Channel: 58696371
FPS: 1998.94
Samples per second: 499733
Expected samples/s: 500000
Events: 8040
Events per second: 68.4515
jumped back (PMT): 4923
#Frames read, frequency
# 0 frames :334360
# 1 frames :117770
# 2 frames :4867
# 3 frames :1770
# 4 frames :992
# 5 frames :552
# 6 frames :1255
# 7 frames :2146
# 8 frames :1409
# 9 frames :851
# 10 frames :586
# 11 frames :571
# 12 frames :458
# 13 frames :327
# 14 frames :297
# 15 frames :233
# 16 frames :173
# 17 frames :148
# 18 frames :136
# 19 frames :97
# 20 frames :88
# 21 frames :68
# 22 frames :66
# 23 frames :45
# 24 frames :34
# 25 frames :33
# 26 frames :26
# 27 frames :21
# 28 frames :15
# 29 frames :16
# 30 frames :11
# 31 frames :11
# 32 frames :13
# 33 frames :11
# 34 frames :8
# 35 frames :3
# 36 frames :9
# 37 frames :7
# 38 frames :7
# 39 frames :3
# 40 frames :1
# 41 frames :3
# 44 frames :1
# 45 frames :1
# 46 frames :2
# 50 frames :2
# 51 frames :2
# 53 frames :1
# 77 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1751
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0