Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.13.03
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0d9c6e6864c1f069ce9ac6fbe233472f",
"name": "M1_20170605_1526_dsRNA_I3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:13:22"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8054 (max 8055) in '8054'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4175
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.83982e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 29 mean_circ: 0.920318
Ran for 1550 frames Cell Events: 44 mean_circ: 0.933159
Ran for 2050 frames Cell Events: 70 mean_circ: 0.938786
Ran for 2550 frames Cell Events: 93 mean_circ: 0.94139
Ran for 3050 frames Cell Events: 107 mean_circ: 0.942272
Ran for 3550 frames Cell Events: 119 mean_circ: 0.943243
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Ran for 5050 frames Cell Events: 163 mean_circ: 0.943486
Ran for 5550 frames Cell Events: 176 mean_circ: 0.943227
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Ran for 6550 frames Cell Events: 195 mean_circ: 0.942859
Ran for 7050 frames Cell Events: 209 mean_circ: 0.94311
Ran for 7550 frames Cell Events: 226 mean_circ: 0.942509
Ran for 8050 frames Cell Events: 246 mean_circ: 0.941986
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Ran for 13550 frames Cell Events: 439 mean_circ: 0.940176
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Ran for 73550 frames Cell Events: 2619 mean_circ: 0.940102
Ran for 74550 frames Cell Events: 2647 mean_circ: 0.940071
Ran for 75050 frames Cell Events: 2664 mean_circ: 0.940079
Ran for 75550 frames Cell Events: 2692 mean_circ: 0.940107
Ran for 76050 frames Cell Events: 2707 mean_circ: 0.940122
Ran for 76550 frames Cell Events: 2729 mean_circ: 0.940103
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Ran for 77550 frames Cell Events: 2766 mean_circ: 0.940092
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Ran for 78550 frames Cell Events: 2801 mean_circ: 0.940259
Ran for 79050 frames Cell Events: 2821 mean_circ: 0.940369
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Ran for 80550 frames Cell Events: 2881 mean_circ: 0.940479
Ran for 82550 frames Cell Events: 2934 mean_circ: 0.940511
Ran for 83050 frames Cell Events: 2956 mean_circ: 0.940624
Ran for 83550 frames Cell Events: 2985 mean_circ: 0.940605
Ran for 85050 frames Cell Events: 3040 mean_circ: 0.940602
Ran for 85550 frames Cell Events: 3057 mean_circ: 0.940673
Ran for 86050 frames Cell Events: 3069 mean_circ: 0.940727
Ran for 86550 frames Cell Events: 3080 mean_circ: 0.94073
Ran for 87050 frames Cell Events: 3109 mean_circ: 0.940743
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Ran for 90050 frames Cell Events: 3228 mean_circ: 0.940703
Ran for 90550 frames Cell Events: 3241 mean_circ: 0.94072
Ran for 91050 frames Cell Events: 3262 mean_circ: 0.940722
Ran for 91550 frames Cell Events: 3295 mean_circ: 0.940653
Ran for 92050 frames Cell Events: 3310 mean_circ: 0.940616
Ran for 92550 frames Cell Events: 3331 mean_circ: 0.940537
Ran for 93050 frames Cell Events: 3351 mean_circ: 0.94052
Ran for 93550 frames Cell Events: 3365 mean_circ: 0.940363
Ran for 94050 frames Cell Events: 3387 mean_circ: 0.940419
Ran for 96050 frames Cell Events: 3468 mean_circ: 0.940366
Ran for 96550 frames Cell Events: 3485 mean_circ: 0.940418
Ran for 97550 frames Cell Events: 3519 mean_circ: 0.940537
Ran for 98050 frames Cell Events: 3529 mean_circ: 0.940568
Ran for 99050 frames Cell Events: 3563 mean_circ: 0.940595
Ran for 100550 frames Cell Events: 3613 mean_circ: 0.940594
Ran for 101050 frames Cell Events: 3624 mean_circ: 0.940622
Ran for 103050 frames Cell Events: 3702 mean_circ: 0.940689
Ran for 103550 frames Cell Events: 3717 mean_circ: 0.940609
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Ran for 106050 frames Cell Events: 3810 mean_circ: 0.940743
Ran for 106550 frames Cell Events: 3822 mean_circ: 0.940784
Ran for 107550 frames Cell Events: 3852 mean_circ: 0.940874
Ran for 108050 frames Cell Events: 3865 mean_circ: 0.940848
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Ran for 120050 frames Cell Events: 4274 mean_circ: 0.940658
Ran for 121050 frames Cell Events: 4306 mean_circ: 0.940638
Ran for 121550 frames Cell Events: 4318 mean_circ: 0.940676
Ran for 122050 frames Cell Events: 4332 mean_circ: 0.940662
Ran for 122550 frames Cell Events: 4348 mean_circ: 0.940678
Ran for 123550 frames Cell Events: 4371 mean_circ: 0.940713
Ran for 124050 frames Cell Events: 4391 mean_circ: 0.940744
Ran for 124550 frames Cell Events: 4403 mean_circ: 0.940804
Ran for 125050 frames Cell Events: 4415 mean_circ: 0.940835
Ran for 125550 frames Cell Events: 4436 mean_circ: 0.940832
Ran for 126050 frames Cell Events: 4445 mean_circ: 0.940813
Ran for 126550 frames Cell Events: 4458 mean_circ: 0.940858
Ran for 127550 frames Cell Events: 4490 mean_circ: 0.940844
Ran for 128050 frames Cell Events: 4508 mean_circ: 0.940875
Ran for 128550 frames Cell Events: 4526 mean_circ: 0.940911
Ran for 130050 frames Cell Events: 4580 mean_circ: 0.940923
Ran for 131050 frames Cell Events: 4627 mean_circ: 0.940951
Ran for 131550 frames Cell Events: 4644 mean_circ: 0.940984
Ran for 132050 frames Cell Events: 4666 mean_circ: 0.941006
Ran for 132550 frames Cell Events: 4678 mean_circ: 0.941015
Ran for 133550 frames Cell Events: 4706 mean_circ: 0.941055
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Ran for 135550 frames Cell Events: 4766 mean_circ: 0.941056
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Ran for 138050 frames Cell Events: 4863 mean_circ: 0.941104
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Ran for 142550 frames Cell Events: 5047 mean_circ: 0.941207
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Ran for 181550 frames Cell Events: 6441 mean_circ: 0.941581
Ran for 182550 frames Cell Events: 6473 mean_circ: 0.941553
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Ran for 196550 frames Cell Events: 6993 mean_circ: 0.941586
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Ran for 199050 frames Cell Events: 7076 mean_circ: 0.941577
Ran for 199550 frames Cell Events: 7091 mean_circ: 0.941594
Ran for 200050 frames Cell Events: 7109 mean_circ: 0.941579
Ran for 201050 frames Cell Events: 7147 mean_circ: 0.941606
Ran for 201550 frames Cell Events: 7162 mean_circ: 0.941607
Ran for 202050 frames Cell Events: 7175 mean_circ: 0.941629
Ran for 202550 frames Cell Events: 7181 mean_circ: 0.941642
Ran for 203550 frames Cell Events: 7214 mean_circ: 0.941616
Ran for 204550 frames Cell Events: 7240 mean_circ: 0.941623
Ran for 205550 frames Cell Events: 7283 mean_circ: 0.941567
Ran for 207050 frames Cell Events: 7333 mean_circ: 0.94158
Ran for 207550 frames Cell Events: 7340 mean_circ: 0.941578
Ran for 208550 frames Cell Events: 7367 mean_circ: 0.941599
Ran for 209050 frames Cell Events: 7376 mean_circ: 0.941608
Ran for 209550 frames Cell Events: 7392 mean_circ: 0.941641
Ran for 210550 frames Cell Events: 7436 mean_circ: 0.941656
Ran for 211550 frames Cell Events: 7478 mean_circ: 0.941616
Ran for 212050 frames Cell Events: 7488 mean_circ: 0.9416
Ran for 212550 frames Cell Events: 7500 mean_circ: 0.94161
Ran for 214050 frames Cell Events: 7559 mean_circ: 0.941561
Ran for 214550 frames Cell Events: 7573 mean_circ: 0.941552
Ran for 216550 frames Cell Events: 7665 mean_circ: 0.941504
Ran for 217550 frames Cell Events: 7711 mean_circ: 0.94146
Ran for 218050 frames Cell Events: 7736 mean_circ: 0.941486
Ran for 218550 frames Cell Events: 7752 mean_circ: 0.941485
Ran for 219550 frames Cell Events: 7786 mean_circ: 0.94147
Ran for 223050 frames Cell Events: 7931 mean_circ: 0.941519
Ran for 223550 frames Cell Events: 7946 mean_circ: 0.941512
Ran for 224550 frames Cell Events: 7990 mean_circ: 0.941491
End this measurment..
Stopping time: 1.83993e+009
****************************************
Measurement finished
Mean Circularitiy: 0.941414
Mean Area: 629.489
Mean FL-1 intensity: 3247.18
Mean FL-2 intensity: 4012.57
Mean FL-3 intensity: 0
frames processed: 224306
Total Time: 112929ms
Number of Runs (new bg img) 0
Frames collected: 225703
Skipped frames: 21732
Rescued Frames: 1144492
Samples DAQ: 56427275
Samples written: 112851658
Samples per Channel: 56425829
FPS: 1998.63
Samples per second: 499657
Expected samples/s: 500000
Events: 8055
Events per second: 71.328
jumped back (PMT): 4574
#Frames read, frequency
# 0 frames :318223
# 1 frames :116700
# 2 frames :4704
# 3 frames :1593
# 4 frames :866
# 5 frames :405
# 6 frames :1810
# 7 frames :1644
# 8 frames :1303
# 9 frames :689
# 10 frames :655
# 11 frames :463
# 12 frames :464
# 13 frames :309
# 14 frames :280
# 15 frames :205
# 16 frames :161
# 17 frames :157
# 18 frames :140
# 19 frames :89
# 20 frames :70
# 21 frames :69
# 22 frames :51
# 23 frames :29
# 24 frames :36
# 25 frames :31
# 26 frames :20
# 27 frames :20
# 28 frames :15
# 29 frames :16
# 30 frames :13
# 31 frames :12
# 32 frames :9
# 33 frames :9
# 34 frames :5
# 35 frames :9
# 36 frames :3
# 37 frames :4
# 38 frames :3
# 39 frames :1
# 40 frames :5
# 42 frames :1
# 43 frames :2
# 44 frames :2
# 45 frames :2
# 47 frames :2
# 48 frames :1
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 53 frames :1
# 55 frames :1
# 60 frames :1
# 66 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4175
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0