Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.16.36
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "1208a8617d444426ba85afb8fea0e1d8",
"name": "M1_20170605_1615_dsRNA_J3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:16:56"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8025 (max 8026) in '8025'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6395
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.84273e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 13 mean_circ: 0.954709
Ran for 1550 frames Cell Events: 43 mean_circ: 0.94547
Ran for 2050 frames Cell Events: 58 mean_circ: 0.946013
Ran for 2550 frames Cell Events: 67 mean_circ: 0.947877
Ran for 3050 frames Cell Events: 77 mean_circ: 0.946831
Ran for 3550 frames Cell Events: 88 mean_circ: 0.946263
Ran for 4550 frames Cell Events: 126 mean_circ: 0.945503
Ran for 5050 frames Cell Events: 138 mean_circ: 0.946239
Ran for 6050 frames Cell Events: 172 mean_circ: 0.945457
Ran for 6550 frames Cell Events: 192 mean_circ: 0.945745
Ran for 7050 frames Cell Events: 206 mean_circ: 0.945743
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Ran for 10550 frames Cell Events: 289 mean_circ: 0.943671
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Ran for 12050 frames Cell Events: 331 mean_circ: 0.942955
Ran for 12550 frames Cell Events: 348 mean_circ: 0.94297
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Ran for 14050 frames Cell Events: 391 mean_circ: 0.943102
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Ran for 60550 frames Cell Events: 1956 mean_circ: 0.943176
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Ran for 64550 frames Cell Events: 2079 mean_circ: 0.943103
Ran for 65050 frames Cell Events: 2088 mean_circ: 0.943039
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Ran for 71550 frames Cell Events: 2328 mean_circ: 0.943511
Ran for 72550 frames Cell Events: 2362 mean_circ: 0.943553
Ran for 73050 frames Cell Events: 2383 mean_circ: 0.94368
Ran for 73550 frames Cell Events: 2391 mean_circ: 0.943725
Ran for 74050 frames Cell Events: 2412 mean_circ: 0.943621
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Ran for 76550 frames Cell Events: 2513 mean_circ: 0.943757
Ran for 77050 frames Cell Events: 2531 mean_circ: 0.943869
Ran for 78550 frames Cell Events: 2592 mean_circ: 0.943983
Ran for 80050 frames Cell Events: 2652 mean_circ: 0.944068
Ran for 80550 frames Cell Events: 2672 mean_circ: 0.943988
Ran for 81050 frames Cell Events: 2688 mean_circ: 0.944025
Ran for 81550 frames Cell Events: 2711 mean_circ: 0.943984
Ran for 82550 frames Cell Events: 2748 mean_circ: 0.943938
Ran for 83550 frames Cell Events: 2783 mean_circ: 0.943897
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Ran for 85550 frames Cell Events: 2848 mean_circ: 0.943838
Ran for 86550 frames Cell Events: 2894 mean_circ: 0.943904
Ran for 87050 frames Cell Events: 2928 mean_circ: 0.944048
Ran for 88050 frames Cell Events: 2977 mean_circ: 0.944073
Ran for 88550 frames Cell Events: 3000 mean_circ: 0.94402
Ran for 89050 frames Cell Events: 3029 mean_circ: 0.944109
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Ran for 90550 frames Cell Events: 3080 mean_circ: 0.944147
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Ran for 91550 frames Cell Events: 3110 mean_circ: 0.944194
Ran for 92050 frames Cell Events: 3125 mean_circ: 0.944235
Ran for 92550 frames Cell Events: 3141 mean_circ: 0.944319
Ran for 93550 frames Cell Events: 3180 mean_circ: 0.944433
Ran for 94050 frames Cell Events: 3195 mean_circ: 0.944469
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Ran for 103050 frames Cell Events: 3518 mean_circ: 0.944296
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Ran for 104050 frames Cell Events: 3548 mean_circ: 0.944382
Ran for 105550 frames Cell Events: 3605 mean_circ: 0.944449
Ran for 106050 frames Cell Events: 3622 mean_circ: 0.944414
Ran for 106550 frames Cell Events: 3640 mean_circ: 0.944483
Ran for 107050 frames Cell Events: 3655 mean_circ: 0.944446
Ran for 107550 frames Cell Events: 3668 mean_circ: 0.944464
Ran for 108050 frames Cell Events: 3684 mean_circ: 0.944441
Ran for 108550 frames Cell Events: 3695 mean_circ: 0.944401
Ran for 109050 frames Cell Events: 3708 mean_circ: 0.944399
Ran for 110050 frames Cell Events: 3753 mean_circ: 0.944404
Ran for 111050 frames Cell Events: 3785 mean_circ: 0.944439
Ran for 111550 frames Cell Events: 3795 mean_circ: 0.944446
Ran for 112050 frames Cell Events: 3803 mean_circ: 0.94448
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Ran for 117050 frames Cell Events: 3973 mean_circ: 0.944668
Ran for 118050 frames Cell Events: 4008 mean_circ: 0.944648
Ran for 119050 frames Cell Events: 4040 mean_circ: 0.944624
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Ran for 120050 frames Cell Events: 4090 mean_circ: 0.944554
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Ran for 122050 frames Cell Events: 4145 mean_circ: 0.944507
Ran for 123050 frames Cell Events: 4171 mean_circ: 0.944476
Ran for 124550 frames Cell Events: 4244 mean_circ: 0.94455
Ran for 125550 frames Cell Events: 4284 mean_circ: 0.944497
Ran for 126050 frames Cell Events: 4300 mean_circ: 0.944498
Ran for 127050 frames Cell Events: 4329 mean_circ: 0.944486
Ran for 127550 frames Cell Events: 4343 mean_circ: 0.944525
Ran for 128050 frames Cell Events: 4367 mean_circ: 0.9445
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Ran for 131050 frames Cell Events: 4461 mean_circ: 0.94446
Ran for 131550 frames Cell Events: 4471 mean_circ: 0.944421
Ran for 132050 frames Cell Events: 4496 mean_circ: 0.944442
Ran for 133050 frames Cell Events: 4539 mean_circ: 0.94437
Ran for 133550 frames Cell Events: 4561 mean_circ: 0.944355
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Ran for 162050 frames Cell Events: 5680 mean_circ: 0.944455
Ran for 162550 frames Cell Events: 5702 mean_circ: 0.944478
Ran for 163050 frames Cell Events: 5720 mean_circ: 0.944516
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Ran for 168050 frames Cell Events: 5895 mean_circ: 0.944603
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Ran for 169050 frames Cell Events: 5938 mean_circ: 0.944555
Ran for 170050 frames Cell Events: 5978 mean_circ: 0.944539
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Ran for 171050 frames Cell Events: 6027 mean_circ: 0.944567
Ran for 171550 frames Cell Events: 6055 mean_circ: 0.944583
Ran for 173050 frames Cell Events: 6130 mean_circ: 0.944607
Ran for 173550 frames Cell Events: 6156 mean_circ: 0.944622
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Ran for 176550 frames Cell Events: 6265 mean_circ: 0.944537
Ran for 177050 frames Cell Events: 6284 mean_circ: 0.944536
Ran for 177550 frames Cell Events: 6297 mean_circ: 0.944555
Ran for 178050 frames Cell Events: 6313 mean_circ: 0.944549
Ran for 178550 frames Cell Events: 6327 mean_circ: 0.944561
Ran for 179050 frames Cell Events: 6344 mean_circ: 0.944553
Ran for 179550 frames Cell Events: 6362 mean_circ: 0.944532
Ran for 182550 frames Cell Events: 6461 mean_circ: 0.94452
Ran for 183050 frames Cell Events: 6475 mean_circ: 0.944541
Ran for 183550 frames Cell Events: 6487 mean_circ: 0.944512
Ran for 184050 frames Cell Events: 6503 mean_circ: 0.944512
Ran for 185550 frames Cell Events: 6557 mean_circ: 0.94458
Ran for 186050 frames Cell Events: 6574 mean_circ: 0.944572
Ran for 186550 frames Cell Events: 6590 mean_circ: 0.944567
Ran for 189050 frames Cell Events: 6672 mean_circ: 0.944557
Ran for 189550 frames Cell Events: 6687 mean_circ: 0.944545
Ran for 190050 frames Cell Events: 6706 mean_circ: 0.944554
Ran for 190550 frames Cell Events: 6717 mean_circ: 0.944548
Ran for 191550 frames Cell Events: 6752 mean_circ: 0.944516
Ran for 192050 frames Cell Events: 6763 mean_circ: 0.944529
Ran for 192550 frames Cell Events: 6777 mean_circ: 0.944495
Ran for 193050 frames Cell Events: 6792 mean_circ: 0.944501
Ran for 194550 frames Cell Events: 6841 mean_circ: 0.944518
Ran for 195050 frames Cell Events: 6859 mean_circ: 0.944488
Ran for 195550 frames Cell Events: 6879 mean_circ: 0.944494
Ran for 196050 frames Cell Events: 6891 mean_circ: 0.944519
Ran for 196550 frames Cell Events: 6908 mean_circ: 0.944511
Ran for 197050 frames Cell Events: 6924 mean_circ: 0.944504
Ran for 197550 frames Cell Events: 6950 mean_circ: 0.944435
Ran for 198050 frames Cell Events: 6966 mean_circ: 0.944445
Ran for 198550 frames Cell Events: 6979 mean_circ: 0.944446
Ran for 199050 frames Cell Events: 6997 mean_circ: 0.944476
Ran for 199550 frames Cell Events: 7019 mean_circ: 0.944482
Ran for 200050 frames Cell Events: 7034 mean_circ: 0.944482
Ran for 201050 frames Cell Events: 7082 mean_circ: 0.944432
Ran for 202550 frames Cell Events: 7136 mean_circ: 0.944419
Ran for 203050 frames Cell Events: 7149 mean_circ: 0.944436
Ran for 204550 frames Cell Events: 7197 mean_circ: 0.944464
Ran for 205550 frames Cell Events: 7244 mean_circ: 0.944514
Ran for 207050 frames Cell Events: 7306 mean_circ: 0.944539
Ran for 207550 frames Cell Events: 7330 mean_circ: 0.944577
Ran for 208050 frames Cell Events: 7354 mean_circ: 0.944598
Ran for 208550 frames Cell Events: 7379 mean_circ: 0.944621
Ran for 209050 frames Cell Events: 7393 mean_circ: 0.9446
Ran for 210550 frames Cell Events: 7455 mean_circ: 0.944612
Ran for 211050 frames Cell Events: 7473 mean_circ: 0.944595
Ran for 211550 frames Cell Events: 7490 mean_circ: 0.944626
Ran for 212550 frames Cell Events: 7526 mean_circ: 0.94464
Ran for 213050 frames Cell Events: 7546 mean_circ: 0.944655
Ran for 214050 frames Cell Events: 7579 mean_circ: 0.944692
Ran for 214550 frames Cell Events: 7606 mean_circ: 0.944666
Ran for 215550 frames Cell Events: 7649 mean_circ: 0.944583
Ran for 216050 frames Cell Events: 7661 mean_circ: 0.944593
Ran for 216550 frames Cell Events: 7683 mean_circ: 0.944589
Ran for 217050 frames Cell Events: 7703 mean_circ: 0.944595
Ran for 217550 frames Cell Events: 7716 mean_circ: 0.944597
Ran for 218050 frames Cell Events: 7739 mean_circ: 0.944588
Ran for 218550 frames Cell Events: 7751 mean_circ: 0.944583
Ran for 219550 frames Cell Events: 7775 mean_circ: 0.944634
Ran for 221550 frames Cell Events: 7839 mean_circ: 0.944642
Ran for 222550 frames Cell Events: 7879 mean_circ: 0.944641
Ran for 223050 frames Cell Events: 7895 mean_circ: 0.944623
Ran for 226050 frames Cell Events: 8013 mean_circ: 0.944678
End this measurment..
Stopping time: 1.84285e+009
****************************************
Measurement finished
Mean Circularitiy: 0.944679
Mean Area: 625.96
Mean FL-1 intensity: 3329.56
Mean FL-2 intensity: 4185.21
Mean FL-3 intensity: 0
frames processed: 224224
Total Time: 113247ms
Number of Runs (new bg img) 0
Frames collected: 226353
Skipped frames: 10053
Rescued Frames: 461746
Samples DAQ: 56588180
Samples written: 113176034
Samples per Channel: 56588017
FPS: 1998.75
Samples per second: 499685
Expected samples/s: 500000
Events: 8026
Events per second: 70.8714
jumped back (PMT): 4872
#Frames read, frequency
# 0 frames :311882
# 1 frames :123682
# 2 frames :5097
# 3 frames :1684
# 4 frames :847
# 5 frames :1599
# 6 frames :2229
# 7 frames :1545
# 8 frames :931
# 9 frames :541
# 10 frames :529
# 11 frames :409
# 12 frames :367
# 13 frames :279
# 14 frames :200
# 15 frames :170
# 16 frames :124
# 17 frames :111
# 18 frames :83
# 19 frames :71
# 20 frames :45
# 21 frames :50
# 22 frames :33
# 23 frames :23
# 24 frames :22
# 25 frames :15
# 26 frames :15
# 27 frames :15
# 28 frames :13
# 29 frames :12
# 30 frames :7
# 31 frames :4
# 32 frames :4
# 33 frames :4
# 34 frames :9
# 35 frames :2
# 36 frames :5
# 37 frames :3
# 38 frames :3
# 40 frames :2
# 43 frames :4
# 44 frames :1
# 46 frames :1
# 47 frames :2
# 48 frames :1
# 49 frames :1
# 51 frames :1
# 55 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6395
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0