Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.21.35
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5dab63d4861a621a4dc3ca54ecd07af9",
"name": "M1_20170605_1717_dsRNA_L3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:21:55"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8140 (max 8141) in '8140'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6057
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.84645e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 15 mean_circ: 0.946924
Ran for 1050 frames Cell Events: 37 mean_circ: 0.934225
Ran for 1550 frames Cell Events: 55 mean_circ: 0.937074
Ran for 2050 frames Cell Events: 71 mean_circ: 0.935414
Ran for 2550 frames Cell Events: 88 mean_circ: 0.9321
Ran for 3050 frames Cell Events: 102 mean_circ: 0.933056
Ran for 3550 frames Cell Events: 124 mean_circ: 0.936306
Ran for 5050 frames Cell Events: 148 mean_circ: 0.936069
Ran for 5550 frames Cell Events: 166 mean_circ: 0.935648
Ran for 6050 frames Cell Events: 184 mean_circ: 0.935734
Ran for 6550 frames Cell Events: 199 mean_circ: 0.936067
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Ran for 11050 frames Cell Events: 374 mean_circ: 0.936384
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Ran for 72550 frames Cell Events: 2953 mean_circ: 0.935359
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Ran for 76550 frames Cell Events: 3128 mean_circ: 0.935693
Ran for 77050 frames Cell Events: 3149 mean_circ: 0.93578
Ran for 78550 frames Cell Events: 3210 mean_circ: 0.9356
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Ran for 81050 frames Cell Events: 3304 mean_circ: 0.935664
Ran for 81550 frames Cell Events: 3331 mean_circ: 0.935606
Ran for 82550 frames Cell Events: 3365 mean_circ: 0.935572
Ran for 84050 frames Cell Events: 3417 mean_circ: 0.93545
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Ran for 86550 frames Cell Events: 3492 mean_circ: 0.935596
Ran for 87050 frames Cell Events: 3512 mean_circ: 0.935659
Ran for 87550 frames Cell Events: 3538 mean_circ: 0.935735
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Ran for 92050 frames Cell Events: 3715 mean_circ: 0.935943
Ran for 92550 frames Cell Events: 3739 mean_circ: 0.935953
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Ran for 94050 frames Cell Events: 3798 mean_circ: 0.935863
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Ran for 97050 frames Cell Events: 3940 mean_circ: 0.936054
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Ran for 120550 frames Cell Events: 4844 mean_circ: 0.935645
Ran for 122050 frames Cell Events: 4916 mean_circ: 0.935687
Ran for 123050 frames Cell Events: 4965 mean_circ: 0.935657
Ran for 125050 frames Cell Events: 5050 mean_circ: 0.93581
Ran for 125550 frames Cell Events: 5067 mean_circ: 0.935824
Ran for 126550 frames Cell Events: 5115 mean_circ: 0.935748
Ran for 127050 frames Cell Events: 5141 mean_circ: 0.935722
Ran for 128050 frames Cell Events: 5181 mean_circ: 0.9357
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Ran for 130050 frames Cell Events: 5252 mean_circ: 0.935665
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Ran for 131050 frames Cell Events: 5295 mean_circ: 0.935756
Ran for 131550 frames Cell Events: 5313 mean_circ: 0.935765
Ran for 132050 frames Cell Events: 5328 mean_circ: 0.935773
Ran for 132550 frames Cell Events: 5337 mean_circ: 0.935736
Ran for 134050 frames Cell Events: 5409 mean_circ: 0.935682
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Ran for 192550 frames Cell Events: 7856 mean_circ: 0.935662
Ran for 193050 frames Cell Events: 7880 mean_circ: 0.935689
Ran for 195050 frames Cell Events: 7971 mean_circ: 0.935669
Ran for 195550 frames Cell Events: 7987 mean_circ: 0.935662
Ran for 196050 frames Cell Events: 8004 mean_circ: 0.935622
Ran for 197050 frames Cell Events: 8040 mean_circ: 0.935614
Ran for 198050 frames Cell Events: 8078 mean_circ: 0.935654
Ran for 198550 frames Cell Events: 8101 mean_circ: 0.935632
Ran for 199550 frames Cell Events: 8138 mean_circ: 0.935666
End this measurment..
Stopping time: 1.84655e+009
****************************************
Measurement finished
Mean Circularitiy: 0.935674
Mean Area: 619.985
Mean FL-1 intensity: 3235.07
Mean FL-2 intensity: 4368.12
Mean FL-3 intensity: 0
frames processed: 196898
Total Time: 99947.6ms
Number of Runs (new bg img) 0
Frames collected: 199721
Skipped frames: 27833
Rescued Frames: 1263715
Samples DAQ: 49930167
Samples written: 99860140
Samples per Channel: 49930070
FPS: 1998.26
Samples per second: 499562
Expected samples/s: 500000
Events: 8141
Events per second: 81.4527
jumped back (PMT): 19
#Frames read, frequency
# 0 frames :277995
# 1 frames :105835
# 2 frames :4184
# 3 frames :1443
# 4 frames :663
# 5 frames :2284
# 6 frames :2258
# 7 frames :1380
# 8 frames :715
# 9 frames :467
# 10 frames :495
# 11 frames :356
# 12 frames :270
# 13 frames :199
# 14 frames :161
# 15 frames :110
# 16 frames :97
# 17 frames :90
# 18 frames :60
# 19 frames :58
# 20 frames :42
# 21 frames :40
# 22 frames :38
# 23 frames :24
# 24 frames :17
# 25 frames :18
# 26 frames :14
# 27 frames :10
# 28 frames :10
# 29 frames :14
# 30 frames :10
# 31 frames :7
# 32 frames :8
# 33 frames :4
# 34 frames :5
# 35 frames :6
# 36 frames :3
# 37 frames :3
# 39 frames :3
# 40 frames :1
# 41 frames :2
# 42 frames :2
# 43 frames :1
# 45 frames :2
# 47 frames :1
# 49 frames :1
# 63 frames :1
# 73 frames :1
# 80 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6057
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0