Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.28.14
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "248a2bad36f830be676fa3fa6529be13",
"name": "M1_20170605_1837_dsRNA_L7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:28:34"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8176 (max 8177) in '8176'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6763
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.85129e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 120 mean_circ: 0.934258
Ran for 4050 frames Cell Events: 213 mean_circ: 0.936591
Ran for 4550 frames Cell Events: 240 mean_circ: 0.937187
Ran for 5050 frames Cell Events: 275 mean_circ: 0.936383
Ran for 5550 frames Cell Events: 303 mean_circ: 0.936383
Ran for 6050 frames Cell Events: 343 mean_circ: 0.937318
Ran for 7550 frames Cell Events: 441 mean_circ: 0.935125
Ran for 8550 frames Cell Events: 495 mean_circ: 0.935548
Ran for 9550 frames Cell Events: 543 mean_circ: 0.935607
Ran for 10050 frames Cell Events: 565 mean_circ: 0.935125
Ran for 11050 frames Cell Events: 620 mean_circ: 0.935652
Ran for 11550 frames Cell Events: 649 mean_circ: 0.934973
Ran for 12050 frames Cell Events: 680 mean_circ: 0.935318
Ran for 12550 frames Cell Events: 703 mean_circ: 0.935451
Ran for 13050 frames Cell Events: 724 mean_circ: 0.935571
Ran for 13550 frames Cell Events: 767 mean_circ: 0.935802
Ran for 14050 frames Cell Events: 793 mean_circ: 0.935879
Ran for 14550 frames Cell Events: 815 mean_circ: 0.935771
Ran for 15050 frames Cell Events: 843 mean_circ: 0.935845
Ran for 16550 frames Cell Events: 936 mean_circ: 0.936867
Ran for 17050 frames Cell Events: 970 mean_circ: 0.93661
Ran for 17550 frames Cell Events: 1009 mean_circ: 0.936732
Ran for 18050 frames Cell Events: 1043 mean_circ: 0.936472
Ran for 19050 frames Cell Events: 1108 mean_circ: 0.935716
Ran for 19550 frames Cell Events: 1139 mean_circ: 0.935421
Ran for 20050 frames Cell Events: 1161 mean_circ: 0.935524
Ran for 20550 frames Cell Events: 1187 mean_circ: 0.93573
Ran for 21050 frames Cell Events: 1212 mean_circ: 0.935957
Ran for 22550 frames Cell Events: 1317 mean_circ: 0.936137
Ran for 23550 frames Cell Events: 1386 mean_circ: 0.93606
Ran for 24050 frames Cell Events: 1421 mean_circ: 0.935851
Ran for 24550 frames Cell Events: 1449 mean_circ: 0.935896
Ran for 25050 frames Cell Events: 1484 mean_circ: 0.935991
Ran for 25550 frames Cell Events: 1529 mean_circ: 0.935984
Ran for 27050 frames Cell Events: 1637 mean_circ: 0.936051
Ran for 27550 frames Cell Events: 1661 mean_circ: 0.935898
Ran for 28050 frames Cell Events: 1687 mean_circ: 0.93598
Ran for 28550 frames Cell Events: 1719 mean_circ: 0.935972
Ran for 29050 frames Cell Events: 1768 mean_circ: 0.935407
Ran for 29550 frames Cell Events: 1807 mean_circ: 0.935334
Ran for 30050 frames Cell Events: 1826 mean_circ: 0.935443
Ran for 31050 frames Cell Events: 1892 mean_circ: 0.935791
Ran for 32050 frames Cell Events: 1949 mean_circ: 0.935771
Ran for 33050 frames Cell Events: 2019 mean_circ: 0.935856
Ran for 34050 frames Cell Events: 2093 mean_circ: 0.935742
Ran for 35050 frames Cell Events: 2161 mean_circ: 0.93575
Ran for 35550 frames Cell Events: 2191 mean_circ: 0.935697
Ran for 36050 frames Cell Events: 2219 mean_circ: 0.935739
Ran for 37550 frames Cell Events: 2317 mean_circ: 0.93586
Ran for 38550 frames Cell Events: 2367 mean_circ: 0.936023
Ran for 39050 frames Cell Events: 2391 mean_circ: 0.935774
Ran for 39550 frames Cell Events: 2413 mean_circ: 0.93581
Ran for 40050 frames Cell Events: 2445 mean_circ: 0.935642
Ran for 41050 frames Cell Events: 2523 mean_circ: 0.93578
Ran for 41550 frames Cell Events: 2550 mean_circ: 0.935524
Ran for 42550 frames Cell Events: 2598 mean_circ: 0.935467
Ran for 43050 frames Cell Events: 2625 mean_circ: 0.935406
Ran for 43550 frames Cell Events: 2647 mean_circ: 0.9353
Ran for 44050 frames Cell Events: 2684 mean_circ: 0.935408
Ran for 45050 frames Cell Events: 2730 mean_circ: 0.935205
Ran for 45550 frames Cell Events: 2759 mean_circ: 0.935119
Ran for 46050 frames Cell Events: 2795 mean_circ: 0.935209
Ran for 46550 frames Cell Events: 2839 mean_circ: 0.935302
Ran for 47050 frames Cell Events: 2865 mean_circ: 0.935331
Ran for 47550 frames Cell Events: 2889 mean_circ: 0.935379
Ran for 48050 frames Cell Events: 2919 mean_circ: 0.935312
Ran for 50550 frames Cell Events: 3038 mean_circ: 0.935146
Ran for 51050 frames Cell Events: 3064 mean_circ: 0.93507
Ran for 51550 frames Cell Events: 3116 mean_circ: 0.935062
Ran for 52050 frames Cell Events: 3136 mean_circ: 0.934936
Ran for 53550 frames Cell Events: 3247 mean_circ: 0.935143
Ran for 54550 frames Cell Events: 3299 mean_circ: 0.935078
Ran for 55050 frames Cell Events: 3332 mean_circ: 0.935147
Ran for 56550 frames Cell Events: 3428 mean_circ: 0.934909
Ran for 57050 frames Cell Events: 3467 mean_circ: 0.935016
Ran for 57550 frames Cell Events: 3496 mean_circ: 0.934923
Ran for 59050 frames Cell Events: 3580 mean_circ: 0.934884
Ran for 60050 frames Cell Events: 3641 mean_circ: 0.934947
Ran for 60550 frames Cell Events: 3671 mean_circ: 0.934908
Ran for 61050 frames Cell Events: 3713 mean_circ: 0.934908
Ran for 61550 frames Cell Events: 3737 mean_circ: 0.934971
Ran for 62050 frames Cell Events: 3769 mean_circ: 0.935058
Ran for 62550 frames Cell Events: 3803 mean_circ: 0.935036
Ran for 63050 frames Cell Events: 3840 mean_circ: 0.935115
Ran for 64550 frames Cell Events: 3948 mean_circ: 0.935114
Ran for 65050 frames Cell Events: 3975 mean_circ: 0.93512
Ran for 66550 frames Cell Events: 4069 mean_circ: 0.935212
Ran for 67550 frames Cell Events: 4122 mean_circ: 0.93512
Ran for 69050 frames Cell Events: 4234 mean_circ: 0.935146
Ran for 69550 frames Cell Events: 4265 mean_circ: 0.935162
Ran for 70550 frames Cell Events: 4343 mean_circ: 0.934984
Ran for 71050 frames Cell Events: 4370 mean_circ: 0.934989
Ran for 72050 frames Cell Events: 4434 mean_circ: 0.934977
Ran for 72550 frames Cell Events: 4456 mean_circ: 0.934952
Ran for 73050 frames Cell Events: 4479 mean_circ: 0.934931
Ran for 75050 frames Cell Events: 4599 mean_circ: 0.935093
Ran for 75550 frames Cell Events: 4623 mean_circ: 0.935086
Ran for 77550 frames Cell Events: 4756 mean_circ: 0.935182
Ran for 78550 frames Cell Events: 4835 mean_circ: 0.93514
Ran for 79050 frames Cell Events: 4867 mean_circ: 0.935193
Ran for 79550 frames Cell Events: 4891 mean_circ: 0.935244
Ran for 80550 frames Cell Events: 4962 mean_circ: 0.935221
Ran for 81050 frames Cell Events: 5001 mean_circ: 0.935259
Ran for 82050 frames Cell Events: 5071 mean_circ: 0.935282
Ran for 83050 frames Cell Events: 5141 mean_circ: 0.93515
Ran for 84550 frames Cell Events: 5276 mean_circ: 0.934926
Ran for 85050 frames Cell Events: 5325 mean_circ: 0.934934
Ran for 85550 frames Cell Events: 5359 mean_circ: 0.934913
Ran for 86050 frames Cell Events: 5400 mean_circ: 0.934944
Ran for 86550 frames Cell Events: 5441 mean_circ: 0.934784
Ran for 87050 frames Cell Events: 5473 mean_circ: 0.934809
Ran for 89050 frames Cell Events: 5590 mean_circ: 0.934647
Ran for 90550 frames Cell Events: 5679 mean_circ: 0.93464
Ran for 92050 frames Cell Events: 5780 mean_circ: 0.934622
Ran for 93050 frames Cell Events: 5830 mean_circ: 0.93459
Ran for 93550 frames Cell Events: 5863 mean_circ: 0.934611
Ran for 94050 frames Cell Events: 5897 mean_circ: 0.934618
Ran for 95050 frames Cell Events: 5976 mean_circ: 0.934652
Ran for 96550 frames Cell Events: 6081 mean_circ: 0.934674
Ran for 97550 frames Cell Events: 6126 mean_circ: 0.934624
Ran for 98050 frames Cell Events: 6163 mean_circ: 0.934649
Ran for 100050 frames Cell Events: 6242 mean_circ: 0.934705
Ran for 102050 frames Cell Events: 6403 mean_circ: 0.93471
Ran for 102550 frames Cell Events: 6447 mean_circ: 0.934726
Ran for 104050 frames Cell Events: 6532 mean_circ: 0.93472
Ran for 105050 frames Cell Events: 6604 mean_circ: 0.934743
Ran for 106050 frames Cell Events: 6679 mean_circ: 0.934672
Ran for 108050 frames Cell Events: 6803 mean_circ: 0.934642
Ran for 108550 frames Cell Events: 6818 mean_circ: 0.934618
Ran for 109050 frames Cell Events: 6842 mean_circ: 0.93463
Ran for 110550 frames Cell Events: 6942 mean_circ: 0.934684
Ran for 111050 frames Cell Events: 6981 mean_circ: 0.934666
Ran for 112050 frames Cell Events: 7006 mean_circ: 0.934699
Ran for 113050 frames Cell Events: 7066 mean_circ: 0.934692
Ran for 113550 frames Cell Events: 7090 mean_circ: 0.934729
Ran for 114050 frames Cell Events: 7110 mean_circ: 0.934716
Ran for 114550 frames Cell Events: 7145 mean_circ: 0.934711
Ran for 115050 frames Cell Events: 7176 mean_circ: 0.934719
Ran for 116550 frames Cell Events: 7233 mean_circ: 0.934758
Ran for 117050 frames Cell Events: 7278 mean_circ: 0.934762
Ran for 118550 frames Cell Events: 7401 mean_circ: 0.934752
Ran for 119050 frames Cell Events: 7432 mean_circ: 0.934746
Ran for 119550 frames Cell Events: 7457 mean_circ: 0.934769
Ran for 122550 frames Cell Events: 7657 mean_circ: 0.934784
Ran for 123550 frames Cell Events: 7713 mean_circ: 0.934729
Ran for 125050 frames Cell Events: 7806 mean_circ: 0.934651
Ran for 126050 frames Cell Events: 7871 mean_circ: 0.934648
Ran for 127050 frames Cell Events: 7937 mean_circ: 0.934642
Ran for 127550 frames Cell Events: 7966 mean_circ: 0.934673
Ran for 128050 frames Cell Events: 7997 mean_circ: 0.934659
Ran for 128550 frames Cell Events: 8029 mean_circ: 0.93467
Ran for 129550 frames Cell Events: 8091 mean_circ: 0.934723
End this measurment..
Stopping time: 1.85136e+009
****************************************
Measurement finished
Mean Circularitiy: 0.934717
Mean Area: 613.263
Mean FL-1 intensity: 3333.57
Mean FL-2 intensity: 4180.41
Mean FL-3 intensity: 0
frames processed: 128350
Total Time: 65451.9ms
Number of Runs (new bg img) 0
Frames collected: 130746
Skipped frames: 46262
Rescued Frames: 2368344
Samples DAQ: 32687610
Samples written: 65373028
Samples per Channel: 32686514
FPS: 1997.59
Samples per second: 499398
Expected samples/s: 500000
Events: 8177
Events per second: 124.931
jumped back (PMT): 13
#Frames read, frequency
# 0 frames :193664
# 1 frames :55924
# 2 frames :2514
# 3 frames :1044
# 4 frames :407
# 5 frames :2451
# 6 frames :1499
# 7 frames :1178
# 8 frames :576
# 9 frames :334
# 10 frames :280
# 11 frames :300
# 12 frames :260
# 13 frames :204
# 14 frames :161
# 15 frames :83
# 16 frames :102
# 17 frames :59
# 18 frames :53
# 19 frames :45
# 20 frames :38
# 21 frames :31
# 22 frames :27
# 23 frames :24
# 24 frames :18
# 25 frames :20
# 26 frames :5
# 27 frames :13
# 28 frames :13
# 29 frames :7
# 30 frames :10
# 31 frames :7
# 32 frames :8
# 33 frames :2
# 34 frames :1
# 35 frames :2
# 36 frames :1
# 37 frames :2
# 40 frames :1
# 41 frames :1
# 43 frames :1
# 51 frames :2
# 52 frames :1
# 53 frames :1
# 99 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6763
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0