Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.31.02
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b33f43a108ce7bf0a607e52598bf1065",
"name": "M1_20170605_1916_dsRNA_L5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:31:22"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7802 (max 7803) in '7802'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.71
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.8536e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.947719
Ran for 1050 frames Cell Events: 36 mean_circ: 0.943812
Ran for 1550 frames Cell Events: 48 mean_circ: 0.945286
Ran for 2550 frames Cell Events: 80 mean_circ: 0.941676
Ran for 3550 frames Cell Events: 123 mean_circ: 0.937613
Ran for 5550 frames Cell Events: 205 mean_circ: 0.938996
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Ran for 6550 frames Cell Events: 239 mean_circ: 0.936496
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Ran for 10050 frames Cell Events: 339 mean_circ: 0.937016
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Ran for 72550 frames Cell Events: 2362 mean_circ: 0.936954
Ran for 73050 frames Cell Events: 2373 mean_circ: 0.936966
Ran for 73550 frames Cell Events: 2394 mean_circ: 0.937046
Ran for 74050 frames Cell Events: 2407 mean_circ: 0.937064
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Ran for 76050 frames Cell Events: 2474 mean_circ: 0.936921
Ran for 76550 frames Cell Events: 2495 mean_circ: 0.93676
Ran for 77550 frames Cell Events: 2532 mean_circ: 0.936862
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Ran for 81550 frames Cell Events: 2681 mean_circ: 0.937101
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Ran for 83050 frames Cell Events: 2735 mean_circ: 0.937061
Ran for 83550 frames Cell Events: 2750 mean_circ: 0.937102
Ran for 84050 frames Cell Events: 2772 mean_circ: 0.937155
Ran for 85550 frames Cell Events: 2829 mean_circ: 0.936987
Ran for 86050 frames Cell Events: 2848 mean_circ: 0.937043
Ran for 86550 frames Cell Events: 2875 mean_circ: 0.937036
Ran for 87050 frames Cell Events: 2891 mean_circ: 0.937144
Ran for 87550 frames Cell Events: 2903 mean_circ: 0.937141
Ran for 88050 frames Cell Events: 2926 mean_circ: 0.937205
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Ran for 91050 frames Cell Events: 3036 mean_circ: 0.93685
Ran for 92050 frames Cell Events: 3079 mean_circ: 0.93691
Ran for 92550 frames Cell Events: 3101 mean_circ: 0.936991
Ran for 93050 frames Cell Events: 3118 mean_circ: 0.937014
Ran for 94050 frames Cell Events: 3159 mean_circ: 0.936984
Ran for 94550 frames Cell Events: 3176 mean_circ: 0.936938
Ran for 95050 frames Cell Events: 3188 mean_circ: 0.936927
Ran for 96050 frames Cell Events: 3216 mean_circ: 0.93687
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Ran for 97050 frames Cell Events: 3248 mean_circ: 0.936883
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Ran for 98050 frames Cell Events: 3285 mean_circ: 0.936748
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Ran for 100050 frames Cell Events: 3348 mean_circ: 0.936678
Ran for 100550 frames Cell Events: 3365 mean_circ: 0.936726
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Ran for 102550 frames Cell Events: 3430 mean_circ: 0.936773
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Ran for 104050 frames Cell Events: 3470 mean_circ: 0.936724
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Ran for 106550 frames Cell Events: 3555 mean_circ: 0.936753
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Ran for 120550 frames Cell Events: 4031 mean_circ: 0.936771
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Ran for 121550 frames Cell Events: 4075 mean_circ: 0.936793
Ran for 122550 frames Cell Events: 4112 mean_circ: 0.93683
Ran for 123050 frames Cell Events: 4126 mean_circ: 0.936842
Ran for 124050 frames Cell Events: 4152 mean_circ: 0.936863
Ran for 124550 frames Cell Events: 4172 mean_circ: 0.936833
Ran for 126050 frames Cell Events: 4227 mean_circ: 0.936834
Ran for 126550 frames Cell Events: 4245 mean_circ: 0.936869
Ran for 127050 frames Cell Events: 4264 mean_circ: 0.936796
Ran for 129050 frames Cell Events: 4340 mean_circ: 0.936574
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Ran for 131050 frames Cell Events: 4419 mean_circ: 0.936542
Ran for 131550 frames Cell Events: 4436 mean_circ: 0.93648
Ran for 132550 frames Cell Events: 4477 mean_circ: 0.936416
Ran for 133050 frames Cell Events: 4493 mean_circ: 0.936396
Ran for 134050 frames Cell Events: 4523 mean_circ: 0.936413
Ran for 135050 frames Cell Events: 4561 mean_circ: 0.936401
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Ran for 141050 frames Cell Events: 4770 mean_circ: 0.936216
Ran for 141550 frames Cell Events: 4782 mean_circ: 0.936239
Ran for 142050 frames Cell Events: 4796 mean_circ: 0.936262
Ran for 142550 frames Cell Events: 4816 mean_circ: 0.936252
Ran for 143550 frames Cell Events: 4840 mean_circ: 0.936272
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Ran for 171550 frames Cell Events: 5785 mean_circ: 0.936097
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Ran for 173050 frames Cell Events: 5830 mean_circ: 0.936108
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Ran for 183550 frames Cell Events: 6241 mean_circ: 0.936094
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Ran for 186550 frames Cell Events: 6354 mean_circ: 0.936125
Ran for 187050 frames Cell Events: 6369 mean_circ: 0.936142
Ran for 187550 frames Cell Events: 6383 mean_circ: 0.936158
Ran for 188050 frames Cell Events: 6402 mean_circ: 0.936171
Ran for 188550 frames Cell Events: 6422 mean_circ: 0.936199
Ran for 189050 frames Cell Events: 6438 mean_circ: 0.936201
Ran for 190050 frames Cell Events: 6466 mean_circ: 0.936182
Ran for 191050 frames Cell Events: 6509 mean_circ: 0.936161
Ran for 192050 frames Cell Events: 6545 mean_circ: 0.93617
Ran for 192550 frames Cell Events: 6573 mean_circ: 0.936185
Ran for 193050 frames Cell Events: 6596 mean_circ: 0.936142
Ran for 194050 frames Cell Events: 6624 mean_circ: 0.936092
Ran for 194550 frames Cell Events: 6643 mean_circ: 0.936089
Ran for 195050 frames Cell Events: 6662 mean_circ: 0.936124
Ran for 197050 frames Cell Events: 6706 mean_circ: 0.935922
Ran for 197550 frames Cell Events: 6721 mean_circ: 0.935926
Ran for 198050 frames Cell Events: 6733 mean_circ: 0.9359
Ran for 198550 frames Cell Events: 6746 mean_circ: 0.935928
Ran for 199550 frames Cell Events: 6791 mean_circ: 0.935875
Ran for 200050 frames Cell Events: 6813 mean_circ: 0.935937
Ran for 200550 frames Cell Events: 6839 mean_circ: 0.935927
Ran for 201050 frames Cell Events: 6863 mean_circ: 0.935921
Ran for 201550 frames Cell Events: 6875 mean_circ: 0.935926
Ran for 202050 frames Cell Events: 6892 mean_circ: 0.935869
Ran for 203050 frames Cell Events: 6937 mean_circ: 0.93585
Ran for 203550 frames Cell Events: 6956 mean_circ: 0.93584
Ran for 204050 frames Cell Events: 6977 mean_circ: 0.935823
Ran for 204550 frames Cell Events: 6995 mean_circ: 0.935844
Ran for 205550 frames Cell Events: 7038 mean_circ: 0.935774
Ran for 206050 frames Cell Events: 7058 mean_circ: 0.935775
Ran for 206550 frames Cell Events: 7073 mean_circ: 0.935775
Ran for 207550 frames Cell Events: 7113 mean_circ: 0.93576
Ran for 208050 frames Cell Events: 7133 mean_circ: 0.935765
Ran for 208550 frames Cell Events: 7153 mean_circ: 0.935774
Ran for 209050 frames Cell Events: 7172 mean_circ: 0.935742
Ran for 210050 frames Cell Events: 7208 mean_circ: 0.935751
Ran for 211550 frames Cell Events: 7281 mean_circ: 0.935759
Ran for 212050 frames Cell Events: 7306 mean_circ: 0.935761
Ran for 212550 frames Cell Events: 7324 mean_circ: 0.935735
Ran for 213550 frames Cell Events: 7349 mean_circ: 0.935763
Ran for 214050 frames Cell Events: 7374 mean_circ: 0.9358
Ran for 214550 frames Cell Events: 7382 mean_circ: 0.935816
Ran for 216050 frames Cell Events: 7430 mean_circ: 0.935834
Ran for 217050 frames Cell Events: 7466 mean_circ: 0.935855
Ran for 217550 frames Cell Events: 7494 mean_circ: 0.935852
Ran for 218550 frames Cell Events: 7536 mean_circ: 0.935869
Ran for 219050 frames Cell Events: 7560 mean_circ: 0.935859
Ran for 219550 frames Cell Events: 7575 mean_circ: 0.935873
Ran for 221550 frames Cell Events: 7633 mean_circ: 0.935809
Ran for 222050 frames Cell Events: 7646 mean_circ: 0.935814
Ran for 222550 frames Cell Events: 7666 mean_circ: 0.935833
Ran for 223050 frames Cell Events: 7679 mean_circ: 0.935839
Ran for 223550 frames Cell Events: 7699 mean_circ: 0.9358
Ran for 224050 frames Cell Events: 7712 mean_circ: 0.935819
Ran for 225050 frames Cell Events: 7753 mean_circ: 0.935824
End this measurment..
Stopping time: 1.85371e+009
****************************************
Measurement finished
Mean Circularitiy: 0.93584
Mean Area: 589.111
Mean FL-1 intensity: 3158.82
Mean FL-2 intensity: 4416.99
Mean FL-3 intensity: 0
frames processed: 224243
Total Time: 113289ms
Number of Runs (new bg img) 0
Frames collected: 226388
Skipped frames: 54173
Rescued Frames: 2986682
Samples DAQ: 56596982
Samples written: 113193780
Samples per Channel: 56596890
FPS: 1998.32
Samples per second: 499580
Expected samples/s: 500000
Events: 7803
Events per second: 68.8769
jumped back (PMT): 9
#Frames read, frequency
# 0 frames :306854
# 1 frames :128793
# 2 frames :5079
# 3 frames :1645
# 4 frames :772
# 5 frames :2114
# 6 frames :2281
# 7 frames :1559
# 8 frames :887
# 9 frames :519
# 10 frames :518
# 11 frames :371
# 12 frames :319
# 13 frames :234
# 14 frames :156
# 15 frames :130
# 16 frames :92
# 17 frames :94
# 18 frames :84
# 19 frames :56
# 20 frames :43
# 21 frames :20
# 22 frames :17
# 23 frames :15
# 24 frames :22
# 25 frames :10
# 26 frames :7
# 27 frames :10
# 28 frames :9
# 29 frames :6
# 30 frames :2
# 31 frames :9
# 32 frames :2
# 33 frames :2
# 34 frames :1
# 35 frames :1
# 37 frames :3
# 38 frames :1
# 40 frames :2
# 60 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.71
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0