Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.44.28
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3eaf65ef74abaecdca6ceb93cc7f424a",
"name": "M1_20170606_1527_dsRNA_N7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:44:47"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7155 (max 7156) in '7155'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9396
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.92624e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 25 mean_circ: 0.912634
Ran for 1550 frames Cell Events: 38 mean_circ: 0.922017
Ran for 2050 frames Cell Events: 52 mean_circ: 0.926215
Ran for 2550 frames Cell Events: 68 mean_circ: 0.924449
Ran for 3550 frames Cell Events: 103 mean_circ: 0.924349
Ran for 4050 frames Cell Events: 119 mean_circ: 0.924785
Ran for 4550 frames Cell Events: 129 mean_circ: 0.92061
Ran for 5550 frames Cell Events: 160 mean_circ: 0.920425
Ran for 6050 frames Cell Events: 174 mean_circ: 0.919025
Ran for 6550 frames Cell Events: 187 mean_circ: 0.918934
Ran for 7050 frames Cell Events: 203 mean_circ: 0.918553
Ran for 8050 frames Cell Events: 246 mean_circ: 0.918858
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Ran for 9050 frames Cell Events: 277 mean_circ: 0.917502
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Ran for 10050 frames Cell Events: 300 mean_circ: 0.916138
Ran for 11050 frames Cell Events: 340 mean_circ: 0.915281
Ran for 11550 frames Cell Events: 356 mean_circ: 0.915867
Ran for 12550 frames Cell Events: 375 mean_circ: 0.914969
Ran for 13050 frames Cell Events: 392 mean_circ: 0.915921
Ran for 14050 frames Cell Events: 417 mean_circ: 0.916801
Ran for 14550 frames Cell Events: 431 mean_circ: 0.916999
Ran for 15050 frames Cell Events: 447 mean_circ: 0.917675
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Ran for 35550 frames Cell Events: 1141 mean_circ: 0.91809
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Ran for 40550 frames Cell Events: 1288 mean_circ: 0.917885
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Ran for 67050 frames Cell Events: 2058 mean_circ: 0.916806
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Ran for 68550 frames Cell Events: 2119 mean_circ: 0.916655
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Ran for 72550 frames Cell Events: 2337 mean_circ: 0.917779
Ran for 73050 frames Cell Events: 2349 mean_circ: 0.9178
Ran for 73550 frames Cell Events: 2361 mean_circ: 0.917889
Ran for 74050 frames Cell Events: 2383 mean_circ: 0.917949
Ran for 75050 frames Cell Events: 2421 mean_circ: 0.917831
Ran for 76550 frames Cell Events: 2478 mean_circ: 0.917899
Ran for 77050 frames Cell Events: 2493 mean_circ: 0.917785
Ran for 78050 frames Cell Events: 2531 mean_circ: 0.917592
Ran for 79550 frames Cell Events: 2577 mean_circ: 0.917523
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Ran for 85550 frames Cell Events: 2709 mean_circ: 0.917509
Ran for 86050 frames Cell Events: 2720 mean_circ: 0.917373
Ran for 86550 frames Cell Events: 2744 mean_circ: 0.917447
Ran for 87550 frames Cell Events: 2778 mean_circ: 0.917489
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Ran for 90550 frames Cell Events: 2839 mean_circ: 0.917396
Ran for 91050 frames Cell Events: 2857 mean_circ: 0.917259
Ran for 92050 frames Cell Events: 2872 mean_circ: 0.917265
Ran for 92550 frames Cell Events: 2884 mean_circ: 0.917325
Ran for 93050 frames Cell Events: 2895 mean_circ: 0.91742
Ran for 94550 frames Cell Events: 2964 mean_circ: 0.917353
Ran for 95050 frames Cell Events: 2980 mean_circ: 0.91737
Ran for 95550 frames Cell Events: 2990 mean_circ: 0.917352
Ran for 96050 frames Cell Events: 2994 mean_circ: 0.917382
Ran for 96550 frames Cell Events: 2999 mean_circ: 0.917427
Ran for 97050 frames Cell Events: 3018 mean_circ: 0.917391
Ran for 97550 frames Cell Events: 3033 mean_circ: 0.917567
Ran for 98050 frames Cell Events: 3045 mean_circ: 0.917558
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Ran for 100550 frames Cell Events: 3123 mean_circ: 0.91764
Ran for 101050 frames Cell Events: 3138 mean_circ: 0.917615
Ran for 101550 frames Cell Events: 3156 mean_circ: 0.917587
Ran for 102050 frames Cell Events: 3168 mean_circ: 0.917577
Ran for 104050 frames Cell Events: 3216 mean_circ: 0.917203
Ran for 104550 frames Cell Events: 3235 mean_circ: 0.917118
Ran for 105050 frames Cell Events: 3250 mean_circ: 0.917091
Ran for 106550 frames Cell Events: 3294 mean_circ: 0.917
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Ran for 112550 frames Cell Events: 3449 mean_circ: 0.917053
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Ran for 121050 frames Cell Events: 3750 mean_circ: 0.916735
Ran for 123050 frames Cell Events: 3839 mean_circ: 0.916999
Ran for 124550 frames Cell Events: 3924 mean_circ: 0.917312
Ran for 125550 frames Cell Events: 3966 mean_circ: 0.91732
Ran for 126050 frames Cell Events: 3992 mean_circ: 0.917394
Ran for 126550 frames Cell Events: 4010 mean_circ: 0.917323
Ran for 127550 frames Cell Events: 4045 mean_circ: 0.917294
Ran for 128550 frames Cell Events: 4071 mean_circ: 0.917316
Ran for 129050 frames Cell Events: 4083 mean_circ: 0.917361
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Ran for 130050 frames Cell Events: 4105 mean_circ: 0.917314
Ran for 130550 frames Cell Events: 4118 mean_circ: 0.917337
Ran for 131550 frames Cell Events: 4157 mean_circ: 0.917355
Ran for 132050 frames Cell Events: 4170 mean_circ: 0.917274
Ran for 132550 frames Cell Events: 4183 mean_circ: 0.917352
Ran for 133050 frames Cell Events: 4200 mean_circ: 0.917384
Ran for 133550 frames Cell Events: 4217 mean_circ: 0.91742
Ran for 134050 frames Cell Events: 4233 mean_circ: 0.917426
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Ran for 138050 frames Cell Events: 4325 mean_circ: 0.917531
Ran for 139050 frames Cell Events: 4356 mean_circ: 0.917445
Ran for 140050 frames Cell Events: 4401 mean_circ: 0.917426
Ran for 140550 frames Cell Events: 4418 mean_circ: 0.917418
Ran for 141050 frames Cell Events: 4425 mean_circ: 0.917352
Ran for 141550 frames Cell Events: 4436 mean_circ: 0.917368
Ran for 142550 frames Cell Events: 4474 mean_circ: 0.917451
Ran for 143050 frames Cell Events: 4489 mean_circ: 0.917331
Ran for 143550 frames Cell Events: 4501 mean_circ: 0.917425
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Ran for 146050 frames Cell Events: 4555 mean_circ: 0.917355
Ran for 147050 frames Cell Events: 4582 mean_circ: 0.917345
Ran for 147550 frames Cell Events: 4590 mean_circ: 0.917316
Ran for 148050 frames Cell Events: 4602 mean_circ: 0.917226
Ran for 148550 frames Cell Events: 4618 mean_circ: 0.917161
Ran for 149050 frames Cell Events: 4629 mean_circ: 0.91711
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Ran for 152050 frames Cell Events: 4725 mean_circ: 0.917099
Ran for 153550 frames Cell Events: 4782 mean_circ: 0.916983
Ran for 154050 frames Cell Events: 4796 mean_circ: 0.916965
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Ran for 193050 frames Cell Events: 5965 mean_circ: 0.916156
Ran for 195050 frames Cell Events: 6035 mean_circ: 0.916107
Ran for 196050 frames Cell Events: 6061 mean_circ: 0.916086
Ran for 197550 frames Cell Events: 6104 mean_circ: 0.916026
Ran for 198550 frames Cell Events: 6126 mean_circ: 0.916011
Ran for 199550 frames Cell Events: 6147 mean_circ: 0.916029
Ran for 200550 frames Cell Events: 6198 mean_circ: 0.916034
Ran for 201550 frames Cell Events: 6223 mean_circ: 0.916077
Ran for 202550 frames Cell Events: 6257 mean_circ: 0.916133
Ran for 203050 frames Cell Events: 6271 mean_circ: 0.916149
Ran for 203550 frames Cell Events: 6294 mean_circ: 0.916145
Ran for 204050 frames Cell Events: 6308 mean_circ: 0.916132
Ran for 204550 frames Cell Events: 6333 mean_circ: 0.916137
Ran for 205050 frames Cell Events: 6347 mean_circ: 0.916092
Ran for 206050 frames Cell Events: 6371 mean_circ: 0.916056
Ran for 206550 frames Cell Events: 6384 mean_circ: 0.916107
Ran for 207050 frames Cell Events: 6393 mean_circ: 0.916131
Ran for 208050 frames Cell Events: 6435 mean_circ: 0.916215
Ran for 208550 frames Cell Events: 6447 mean_circ: 0.916217
Ran for 209050 frames Cell Events: 6454 mean_circ: 0.916222
Ran for 211050 frames Cell Events: 6519 mean_circ: 0.91596
Ran for 212050 frames Cell Events: 6555 mean_circ: 0.915959
Ran for 212550 frames Cell Events: 6567 mean_circ: 0.915994
Ran for 213550 frames Cell Events: 6598 mean_circ: 0.916075
Ran for 214050 frames Cell Events: 6612 mean_circ: 0.916135
Ran for 214550 frames Cell Events: 6626 mean_circ: 0.916099
Ran for 215050 frames Cell Events: 6641 mean_circ: 0.916089
Ran for 215550 frames Cell Events: 6653 mean_circ: 0.916098
Ran for 216050 frames Cell Events: 6667 mean_circ: 0.916052
Ran for 216550 frames Cell Events: 6691 mean_circ: 0.916075
Ran for 217550 frames Cell Events: 6724 mean_circ: 0.916046
Ran for 218050 frames Cell Events: 6748 mean_circ: 0.916056
Ran for 218550 frames Cell Events: 6759 mean_circ: 0.916048
Ran for 219050 frames Cell Events: 6770 mean_circ: 0.916061
Ran for 219550 frames Cell Events: 6787 mean_circ: 0.915987
Ran for 220550 frames Cell Events: 6813 mean_circ: 0.915986
Ran for 221050 frames Cell Events: 6824 mean_circ: 0.916016
Ran for 222050 frames Cell Events: 6863 mean_circ: 0.915953
Ran for 223050 frames Cell Events: 6902 mean_circ: 0.915955
Ran for 224050 frames Cell Events: 6919 mean_circ: 0.915971
Ran for 224550 frames Cell Events: 6937 mean_circ: 0.91602
Ran for 226550 frames Cell Events: 6986 mean_circ: 0.916088
Ran for 227050 frames Cell Events: 7006 mean_circ: 0.916082
Ran for 227550 frames Cell Events: 7016 mean_circ: 0.916106
Ran for 228050 frames Cell Events: 7029 mean_circ: 0.916045
Ran for 228550 frames Cell Events: 7039 mean_circ: 0.916033
Ran for 229050 frames Cell Events: 7058 mean_circ: 0.916027
Ran for 230550 frames Cell Events: 7110 mean_circ: 0.915895
Ran for 231050 frames Cell Events: 7130 mean_circ: 0.915875
End this measurment..
Stopping time: 1.92636e+009
****************************************
Measurement finished
Mean Circularitiy: 0.915803
Mean Area: 764.184
Mean FL-1 intensity: 3308.37
Mean FL-2 intensity: 4569.86
Mean FL-3 intensity: 0
frames processed: 225582
Total Time: 116045ms
Number of Runs (new bg img) 0
Frames collected: 231933
Skipped frames: 64056
Rescued Frames: 1513879
Samples DAQ: 57983247
Samples written: 115966334
Samples per Channel: 57983167
FPS: 1998.65
Samples per second: 499662
Expected samples/s: 500000
Events: 7156
Events per second: 61.6659
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :318380
# 1 frames :129671
# 2 frames :4730
# 3 frames :1557
# 4 frames :806
# 5 frames :351
# 6 frames :1798
# 7 frames :1755
# 8 frames :1441
# 9 frames :938
# 10 frames :552
# 11 frames :332
# 12 frames :341
# 13 frames :197
# 14 frames :196
# 15 frames :196
# 16 frames :129
# 17 frames :76
# 18 frames :77
# 19 frames :36
# 20 frames :35
# 21 frames :37
# 22 frames :36
# 23 frames :25
# 24 frames :13
# 25 frames :13
# 26 frames :15
# 27 frames :14
# 28 frames :4
# 29 frames :11
# 30 frames :8
# 31 frames :5
# 32 frames :4
# 33 frames :2
# 34 frames :5
# 35 frames :4
# 36 frames :3
# 37 frames :4
# 38 frames :3
# 39 frames :2
# 40 frames :2
# 41 frames :1
# 42 frames :2
# 43 frames :4
# 45 frames :3
# 46 frames :3
# 48 frames :2
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 53 frames :2
# 69 frames :1
# 86 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9396
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0