Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.49.07
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e7b61ea80a3aeef41ed729fce41eeaaf",
"name": "M1_20170606_1626_dsRNA_O4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:49:27"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8033 (max 8034) in '8033'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0454
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.92978e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 14 mean_circ: 0.951555
Ran for 1550 frames Cell Events: 45 mean_circ: 0.938448
Ran for 3050 frames Cell Events: 88 mean_circ: 0.938564
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Ran for 5050 frames Cell Events: 143 mean_circ: 0.939995
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Ran for 7550 frames Cell Events: 210 mean_circ: 0.94281
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Ran for 92550 frames Cell Events: 2935 mean_circ: 0.941128
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Ran for 94050 frames Cell Events: 2992 mean_circ: 0.941166
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Ran for 100050 frames Cell Events: 3240 mean_circ: 0.941046
Ran for 100550 frames Cell Events: 3253 mean_circ: 0.941041
Ran for 101550 frames Cell Events: 3285 mean_circ: 0.940975
Ran for 102050 frames Cell Events: 3304 mean_circ: 0.941075
Ran for 102550 frames Cell Events: 3316 mean_circ: 0.941108
Ran for 103050 frames Cell Events: 3327 mean_circ: 0.941109
Ran for 103550 frames Cell Events: 3345 mean_circ: 0.941145
Ran for 104050 frames Cell Events: 3354 mean_circ: 0.941112
Ran for 104550 frames Cell Events: 3375 mean_circ: 0.941009
Ran for 105050 frames Cell Events: 3398 mean_circ: 0.940962
Ran for 105550 frames Cell Events: 3413 mean_circ: 0.940905
Ran for 106050 frames Cell Events: 3424 mean_circ: 0.940912
Ran for 108050 frames Cell Events: 3469 mean_circ: 0.940981
Ran for 108550 frames Cell Events: 3486 mean_circ: 0.940956
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Ran for 111050 frames Cell Events: 3573 mean_circ: 0.940875
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Ran for 121550 frames Cell Events: 3934 mean_circ: 0.940679
Ran for 122050 frames Cell Events: 3950 mean_circ: 0.9407
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Ran for 123050 frames Cell Events: 3982 mean_circ: 0.940662
Ran for 123550 frames Cell Events: 4001 mean_circ: 0.940694
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Ran for 126050 frames Cell Events: 4103 mean_circ: 0.940684
Ran for 126550 frames Cell Events: 4121 mean_circ: 0.940677
Ran for 127050 frames Cell Events: 4143 mean_circ: 0.940689
Ran for 128050 frames Cell Events: 4180 mean_circ: 0.940695
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Ran for 131050 frames Cell Events: 4300 mean_circ: 0.940685
Ran for 131550 frames Cell Events: 4313 mean_circ: 0.940673
Ran for 132050 frames Cell Events: 4324 mean_circ: 0.940638
Ran for 132550 frames Cell Events: 4331 mean_circ: 0.940583
Ran for 133050 frames Cell Events: 4347 mean_circ: 0.940584
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Ran for 141550 frames Cell Events: 4620 mean_circ: 0.940629
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Ran for 142550 frames Cell Events: 4649 mean_circ: 0.940638
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Ran for 146050 frames Cell Events: 4781 mean_circ: 0.940683
Ran for 146550 frames Cell Events: 4792 mean_circ: 0.94072
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Ran for 197050 frames Cell Events: 6634 mean_circ: 0.940247
Ran for 197550 frames Cell Events: 6655 mean_circ: 0.940262
Ran for 198550 frames Cell Events: 6689 mean_circ: 0.940281
Ran for 199050 frames Cell Events: 6701 mean_circ: 0.940243
Ran for 200050 frames Cell Events: 6749 mean_circ: 0.940237
Ran for 201050 frames Cell Events: 6781 mean_circ: 0.940189
Ran for 201550 frames Cell Events: 6802 mean_circ: 0.940184
Ran for 203050 frames Cell Events: 6881 mean_circ: 0.940142
Ran for 203550 frames Cell Events: 6898 mean_circ: 0.940123
Ran for 204050 frames Cell Events: 6915 mean_circ: 0.940121
Ran for 205550 frames Cell Events: 6969 mean_circ: 0.940111
Ran for 206050 frames Cell Events: 6985 mean_circ: 0.940164
Ran for 206550 frames Cell Events: 7008 mean_circ: 0.940187
Ran for 207050 frames Cell Events: 7026 mean_circ: 0.940223
Ran for 208550 frames Cell Events: 7097 mean_circ: 0.940184
Ran for 209050 frames Cell Events: 7115 mean_circ: 0.940168
Ran for 210050 frames Cell Events: 7158 mean_circ: 0.940148
Ran for 210550 frames Cell Events: 7173 mean_circ: 0.940146
Ran for 212050 frames Cell Events: 7231 mean_circ: 0.940088
Ran for 212550 frames Cell Events: 7250 mean_circ: 0.940129
Ran for 213050 frames Cell Events: 7263 mean_circ: 0.940131
Ran for 213550 frames Cell Events: 7281 mean_circ: 0.940161
Ran for 214550 frames Cell Events: 7317 mean_circ: 0.940151
Ran for 215050 frames Cell Events: 7335 mean_circ: 0.940137
Ran for 215550 frames Cell Events: 7358 mean_circ: 0.940112
Ran for 216050 frames Cell Events: 7377 mean_circ: 0.940063
Ran for 216550 frames Cell Events: 7392 mean_circ: 0.940091
Ran for 217550 frames Cell Events: 7420 mean_circ: 0.940104
Ran for 218050 frames Cell Events: 7432 mean_circ: 0.940123
Ran for 219050 frames Cell Events: 7482 mean_circ: 0.940183
Ran for 219550 frames Cell Events: 7492 mean_circ: 0.940189
Ran for 220050 frames Cell Events: 7507 mean_circ: 0.940204
Ran for 221050 frames Cell Events: 7556 mean_circ: 0.940242
Ran for 221550 frames Cell Events: 7572 mean_circ: 0.940263
Ran for 222050 frames Cell Events: 7596 mean_circ: 0.940257
Ran for 222550 frames Cell Events: 7610 mean_circ: 0.940261
Ran for 223050 frames Cell Events: 7620 mean_circ: 0.940281
Ran for 223550 frames Cell Events: 7634 mean_circ: 0.940274
Ran for 224550 frames Cell Events: 7670 mean_circ: 0.940263
Ran for 225050 frames Cell Events: 7693 mean_circ: 0.940298
Ran for 227050 frames Cell Events: 7761 mean_circ: 0.940327
Ran for 228050 frames Cell Events: 7792 mean_circ: 0.940311
Ran for 228550 frames Cell Events: 7821 mean_circ: 0.940325
Ran for 230050 frames Cell Events: 7868 mean_circ: 0.940385
Ran for 230550 frames Cell Events: 7882 mean_circ: 0.940389
Ran for 231050 frames Cell Events: 7896 mean_circ: 0.940377
Ran for 231550 frames Cell Events: 7918 mean_circ: 0.940393
Ran for 232050 frames Cell Events: 7935 mean_circ: 0.940407
Ran for 232550 frames Cell Events: 7954 mean_circ: 0.940415
Ran for 233050 frames Cell Events: 7970 mean_circ: 0.940387
Ran for 233550 frames Cell Events: 7997 mean_circ: 0.940379
Ran for 234050 frames Cell Events: 8022 mean_circ: 0.940368
End this measurment..
Stopping time: 1.9299e+009
****************************************
Measurement finished
Mean Circularitiy: 0.940397
Mean Area: 633.315
Mean FL-1 intensity: 3920.45
Mean FL-2 intensity: 5492
Mean FL-3 intensity: 0
frames processed: 220963
Total Time: 117272ms
Number of Runs (new bg img) 0
Frames collected: 234418
Skipped frames: 72574
Rescued Frames: 469122
Samples DAQ: 58606562
Samples written: 117209124
Samples per Channel: 58604562
FPS: 1998.92
Samples per second: 499730
Expected samples/s: 500000
Events: 8034
Events per second: 68.5072
jumped back (PMT): 15
#Frames read, frequency
# 0 frames :330349
# 1 frames :122136
# 2 frames :4176
# 3 frames :1703
# 4 frames :780
# 5 frames :2069
# 6 frames :2103
# 7 frames :1372
# 8 frames :814
# 9 frames :499
# 10 frames :523
# 11 frames :464
# 12 frames :330
# 13 frames :292
# 14 frames :192
# 15 frames :146
# 16 frames :97
# 17 frames :97
# 18 frames :99
# 19 frames :54
# 20 frames :54
# 21 frames :45
# 22 frames :37
# 23 frames :36
# 24 frames :27
# 25 frames :23
# 26 frames :19
# 27 frames :21
# 28 frames :12
# 29 frames :18
# 30 frames :11
# 31 frames :6
# 32 frames :6
# 33 frames :6
# 34 frames :2
# 35 frames :4
# 36 frames :5
# 37 frames :7
# 38 frames :1
# 39 frames :2
# 40 frames :5
# 42 frames :2
# 43 frames :2
# 44 frames :2
# 45 frames :2
# 46 frames :3
# 47 frames :2
# 48 frames :4
# 49 frames :1
# 50 frames :4
# 51 frames :3
# 52 frames :1
# 53 frames :3
# 54 frames :2
# 55 frames :2
# 57 frames :1
# 58 frames :2
# 59 frames :1
# 61 frames :4
# 62 frames :3
# 63 frames :1
# 65 frames :2
# 69 frames :1
# 75 frames :2
# 76 frames :1
# 78 frames :4
# 79 frames :4
# 81 frames :1
# 82 frames :2
# 83 frames :2
# 86 frames :3
# 87 frames :1
# 89 frames :4
# 90 frames :2
# 95 frames :1
# 97 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0454
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0