Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.52.47
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f28b7677ac006e0c2b21b15978f06d31",
"name": "M1_20170606_1723_dsRNA_O8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:53:11"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9428 (max 9429) in '9428'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1466
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.93321e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 15 mean_circ: 0.920393
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Ran for 199050 frames Cell Events: 7799 mean_circ: 0.931613
Ran for 199550 frames Cell Events: 7806 mean_circ: 0.931615
Ran for 201050 frames Cell Events: 7856 mean_circ: 0.931594
Ran for 202050 frames Cell Events: 7919 mean_circ: 0.931625
Ran for 203550 frames Cell Events: 7974 mean_circ: 0.93151
Ran for 204550 frames Cell Events: 8002 mean_circ: 0.93155
Ran for 205050 frames Cell Events: 8019 mean_circ: 0.931523
Ran for 205550 frames Cell Events: 8030 mean_circ: 0.931528
Ran for 206050 frames Cell Events: 8043 mean_circ: 0.931517
Ran for 206550 frames Cell Events: 8059 mean_circ: 0.931528
Ran for 207050 frames Cell Events: 8069 mean_circ: 0.931535
Ran for 207550 frames Cell Events: 8084 mean_circ: 0.9315
Ran for 208550 frames Cell Events: 8136 mean_circ: 0.931539
Ran for 209050 frames Cell Events: 8156 mean_circ: 0.931562
Ran for 209550 frames Cell Events: 8175 mean_circ: 0.931593
Ran for 213550 frames Cell Events: 8322 mean_circ: 0.931527
Ran for 214550 frames Cell Events: 8379 mean_circ: 0.931485
Ran for 215050 frames Cell Events: 8398 mean_circ: 0.93145
Ran for 215550 frames Cell Events: 8416 mean_circ: 0.931476
Ran for 216050 frames Cell Events: 8435 mean_circ: 0.931484
Ran for 216550 frames Cell Events: 8448 mean_circ: 0.931512
Ran for 217050 frames Cell Events: 8466 mean_circ: 0.931532
Ran for 218050 frames Cell Events: 8513 mean_circ: 0.931524
Ran for 218550 frames Cell Events: 8538 mean_circ: 0.931548
Ran for 219050 frames Cell Events: 8558 mean_circ: 0.931526
Ran for 220550 frames Cell Events: 8622 mean_circ: 0.93159
Ran for 221050 frames Cell Events: 8635 mean_circ: 0.9316
Ran for 222550 frames Cell Events: 8684 mean_circ: 0.931609
Ran for 223050 frames Cell Events: 8707 mean_circ: 0.931565
Ran for 223550 frames Cell Events: 8728 mean_circ: 0.931568
Ran for 225550 frames Cell Events: 8789 mean_circ: 0.931487
Ran for 227050 frames Cell Events: 8852 mean_circ: 0.931442
Ran for 227550 frames Cell Events: 8869 mean_circ: 0.9315
Ran for 228050 frames Cell Events: 8889 mean_circ: 0.931527
Ran for 228550 frames Cell Events: 8908 mean_circ: 0.931532
Ran for 229050 frames Cell Events: 8923 mean_circ: 0.93153
Ran for 229550 frames Cell Events: 8940 mean_circ: 0.931535
Ran for 230050 frames Cell Events: 8967 mean_circ: 0.931558
Ran for 231550 frames Cell Events: 9018 mean_circ: 0.931575
Ran for 232050 frames Cell Events: 9034 mean_circ: 0.93158
Ran for 233050 frames Cell Events: 9086 mean_circ: 0.931537
Ran for 233550 frames Cell Events: 9104 mean_circ: 0.931492
Ran for 234550 frames Cell Events: 9139 mean_circ: 0.931551
Ran for 235050 frames Cell Events: 9154 mean_circ: 0.93157
Ran for 235550 frames Cell Events: 9172 mean_circ: 0.93157
Ran for 236050 frames Cell Events: 9195 mean_circ: 0.931571
Ran for 236550 frames Cell Events: 9209 mean_circ: 0.93158
Ran for 237050 frames Cell Events: 9224 mean_circ: 0.931528
Ran for 238050 frames Cell Events: 9281 mean_circ: 0.931585
Ran for 238550 frames Cell Events: 9292 mean_circ: 0.931585
Ran for 239050 frames Cell Events: 9317 mean_circ: 0.931541
Ran for 239550 frames Cell Events: 9334 mean_circ: 0.931547
Ran for 240550 frames Cell Events: 9399 mean_circ: 0.931575
Ran for 241050 frames Cell Events: 9412 mean_circ: 0.931596
End this measurment..
Stopping time: 1.93333e+009
****************************************
Measurement finished
Mean Circularitiy: 0.931606
Mean Area: 635.371
Mean FL-1 intensity: 3355.76
Mean FL-2 intensity: 4998.56
Mean FL-3 intensity: 0
frames processed: 240737
Total Time: 120817ms
Number of Runs (new bg img) 0
Frames collected: 241518
Skipped frames: 90964
Rescued Frames: 1233826
Samples DAQ: 60379443
Samples written: 120758746
Samples per Channel: 60379373
FPS: 1999.05
Samples per second: 499761
Expected samples/s: 500000
Events: 9429
Events per second: 78.0439
jumped back (PMT): 26
#Frames read, frequency
# 0 frames :347544
# 1 frames :115890
# 2 frames :5105
# 3 frames :2119
# 4 frames :1159
# 5 frames :1482
# 6 frames :2075
# 7 frames :1691
# 8 frames :998
# 9 frames :859
# 10 frames :855
# 11 frames :697
# 12 frames :535
# 13 frames :331
# 14 frames :271
# 15 frames :231
# 16 frames :160
# 17 frames :132
# 18 frames :138
# 19 frames :112
# 20 frames :101
# 21 frames :98
# 22 frames :62
# 23 frames :45
# 24 frames :32
# 25 frames :24
# 26 frames :18
# 27 frames :22
# 28 frames :11
# 29 frames :12
# 30 frames :7
# 31 frames :10
# 32 frames :13
# 33 frames :8
# 34 frames :3
# 35 frames :10
# 36 frames :7
# 38 frames :8
# 39 frames :5
# 40 frames :3
# 41 frames :3
# 42 frames :3
# 45 frames :1
# 46 frames :1
# 47 frames :2
# 48 frames :1
# 49 frames :1
# 51 frames :1
# 53 frames :1
# 54 frames :1
# 70 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1466
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0