Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.55.39
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "174c7dbdceeefa0f28aaefe4558cf32a",
"name": "M1_20170606_1806_dsRNA_Z3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:55:59"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8002 (max 8003) in '8002'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2626
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.9358e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 16 mean_circ: 0.894978
Ran for 3550 frames Cell Events: 153 mean_circ: 0.936995
Ran for 4550 frames Cell Events: 202 mean_circ: 0.935592
Ran for 7050 frames Cell Events: 279 mean_circ: 0.936667
Ran for 7550 frames Cell Events: 294 mean_circ: 0.935938
Ran for 8050 frames Cell Events: 314 mean_circ: 0.935018
Ran for 8550 frames Cell Events: 340 mean_circ: 0.935782
Ran for 9550 frames Cell Events: 382 mean_circ: 0.936114
Ran for 10550 frames Cell Events: 429 mean_circ: 0.935788
Ran for 11050 frames Cell Events: 444 mean_circ: 0.936156
Ran for 11550 frames Cell Events: 471 mean_circ: 0.936529
Ran for 12050 frames Cell Events: 486 mean_circ: 0.936548
Ran for 12550 frames Cell Events: 500 mean_circ: 0.936868
Ran for 13050 frames Cell Events: 513 mean_circ: 0.936371
Ran for 13550 frames Cell Events: 535 mean_circ: 0.936371
Ran for 14050 frames Cell Events: 549 mean_circ: 0.936445
Ran for 14550 frames Cell Events: 573 mean_circ: 0.936131
Ran for 15050 frames Cell Events: 600 mean_circ: 0.936128
Ran for 15550 frames Cell Events: 612 mean_circ: 0.936299
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Ran for 18050 frames Cell Events: 704 mean_circ: 0.936421
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Ran for 63550 frames Cell Events: 2469 mean_circ: 0.934625
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Ran for 65550 frames Cell Events: 2542 mean_circ: 0.934543
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Ran for 66550 frames Cell Events: 2589 mean_circ: 0.934445
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Ran for 68550 frames Cell Events: 2646 mean_circ: 0.934316
Ran for 69050 frames Cell Events: 2672 mean_circ: 0.934147
Ran for 69550 frames Cell Events: 2687 mean_circ: 0.934266
Ran for 70050 frames Cell Events: 2702 mean_circ: 0.934341
Ran for 71050 frames Cell Events: 2754 mean_circ: 0.934359
Ran for 72050 frames Cell Events: 2804 mean_circ: 0.934306
Ran for 72550 frames Cell Events: 2823 mean_circ: 0.934289
Ran for 73050 frames Cell Events: 2836 mean_circ: 0.934305
Ran for 74050 frames Cell Events: 2870 mean_circ: 0.934155
Ran for 74550 frames Cell Events: 2897 mean_circ: 0.934205
Ran for 76550 frames Cell Events: 2975 mean_circ: 0.934135
Ran for 77050 frames Cell Events: 2996 mean_circ: 0.934087
Ran for 77550 frames Cell Events: 3013 mean_circ: 0.934107
Ran for 78050 frames Cell Events: 3038 mean_circ: 0.934073
Ran for 78550 frames Cell Events: 3057 mean_circ: 0.934077
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Ran for 80050 frames Cell Events: 3117 mean_circ: 0.933936
Ran for 80550 frames Cell Events: 3132 mean_circ: 0.934005
Ran for 81050 frames Cell Events: 3152 mean_circ: 0.933989
Ran for 81550 frames Cell Events: 3171 mean_circ: 0.933852
Ran for 82550 frames Cell Events: 3208 mean_circ: 0.933789
Ran for 83050 frames Cell Events: 3228 mean_circ: 0.933794
Ran for 83550 frames Cell Events: 3249 mean_circ: 0.933692
Ran for 84050 frames Cell Events: 3271 mean_circ: 0.933691
Ran for 85050 frames Cell Events: 3328 mean_circ: 0.933708
Ran for 85550 frames Cell Events: 3351 mean_circ: 0.933764
Ran for 86550 frames Cell Events: 3393 mean_circ: 0.933997
Ran for 87050 frames Cell Events: 3413 mean_circ: 0.933941
Ran for 87550 frames Cell Events: 3431 mean_circ: 0.933937
Ran for 88050 frames Cell Events: 3459 mean_circ: 0.933952
Ran for 88550 frames Cell Events: 3480 mean_circ: 0.933883
Ran for 89550 frames Cell Events: 3519 mean_circ: 0.933876
Ran for 90050 frames Cell Events: 3536 mean_circ: 0.933924
Ran for 90550 frames Cell Events: 3558 mean_circ: 0.934
Ran for 91550 frames Cell Events: 3601 mean_circ: 0.933977
Ran for 92550 frames Cell Events: 3650 mean_circ: 0.933864
Ran for 93550 frames Cell Events: 3690 mean_circ: 0.933912
Ran for 94050 frames Cell Events: 3711 mean_circ: 0.933935
Ran for 95050 frames Cell Events: 3746 mean_circ: 0.933958
Ran for 95550 frames Cell Events: 3762 mean_circ: 0.933899
Ran for 96050 frames Cell Events: 3778 mean_circ: 0.933846
Ran for 96550 frames Cell Events: 3792 mean_circ: 0.933821
Ran for 97050 frames Cell Events: 3809 mean_circ: 0.933779
Ran for 98050 frames Cell Events: 3860 mean_circ: 0.933627
Ran for 98550 frames Cell Events: 3882 mean_circ: 0.933596
Ran for 101050 frames Cell Events: 4001 mean_circ: 0.933397
Ran for 101550 frames Cell Events: 4018 mean_circ: 0.933466
Ran for 102050 frames Cell Events: 4034 mean_circ: 0.933473
Ran for 102550 frames Cell Events: 4055 mean_circ: 0.933388
Ran for 103550 frames Cell Events: 4088 mean_circ: 0.933277
Ran for 104050 frames Cell Events: 4107 mean_circ: 0.933209
Ran for 104550 frames Cell Events: 4127 mean_circ: 0.933142
Ran for 105050 frames Cell Events: 4145 mean_circ: 0.933213
Ran for 106050 frames Cell Events: 4177 mean_circ: 0.933164
Ran for 106550 frames Cell Events: 4196 mean_circ: 0.93315
Ran for 107050 frames Cell Events: 4224 mean_circ: 0.933202
Ran for 107550 frames Cell Events: 4242 mean_circ: 0.933198
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Ran for 108550 frames Cell Events: 4286 mean_circ: 0.933273
Ran for 109050 frames Cell Events: 4313 mean_circ: 0.933216
Ran for 110050 frames Cell Events: 4352 mean_circ: 0.933316
Ran for 110550 frames Cell Events: 4369 mean_circ: 0.933366
Ran for 111050 frames Cell Events: 4383 mean_circ: 0.933438
Ran for 112050 frames Cell Events: 4429 mean_circ: 0.933506
Ran for 112550 frames Cell Events: 4450 mean_circ: 0.93348
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Ran for 116050 frames Cell Events: 4580 mean_circ: 0.933609
Ran for 118050 frames Cell Events: 4655 mean_circ: 0.933508
Ran for 118550 frames Cell Events: 4669 mean_circ: 0.933481
Ran for 120050 frames Cell Events: 4734 mean_circ: 0.933537
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Ran for 121550 frames Cell Events: 4795 mean_circ: 0.933457
Ran for 122050 frames Cell Events: 4810 mean_circ: 0.933471
Ran for 123050 frames Cell Events: 4849 mean_circ: 0.933344
Ran for 124050 frames Cell Events: 4865 mean_circ: 0.933334
Ran for 125050 frames Cell Events: 4911 mean_circ: 0.933291
Ran for 125550 frames Cell Events: 4931 mean_circ: 0.933336
Ran for 126050 frames Cell Events: 4955 mean_circ: 0.933358
Ran for 126550 frames Cell Events: 4989 mean_circ: 0.933366
Ran for 129050 frames Cell Events: 5100 mean_circ: 0.933228
Ran for 129550 frames Cell Events: 5116 mean_circ: 0.933301
Ran for 130050 frames Cell Events: 5127 mean_circ: 0.933311
Ran for 130550 frames Cell Events: 5149 mean_circ: 0.933256
Ran for 131050 frames Cell Events: 5168 mean_circ: 0.933222
Ran for 132050 frames Cell Events: 5217 mean_circ: 0.933059
Ran for 132550 frames Cell Events: 5238 mean_circ: 0.932998
Ran for 133550 frames Cell Events: 5276 mean_circ: 0.932927
Ran for 134050 frames Cell Events: 5292 mean_circ: 0.932954
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Ran for 135550 frames Cell Events: 5357 mean_circ: 0.932974
Ran for 136050 frames Cell Events: 5382 mean_circ: 0.93301
Ran for 136550 frames Cell Events: 5402 mean_circ: 0.932996
Ran for 137050 frames Cell Events: 5423 mean_circ: 0.932917
Ran for 137550 frames Cell Events: 5445 mean_circ: 0.932955
Ran for 138050 frames Cell Events: 5467 mean_circ: 0.932966
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Ran for 140050 frames Cell Events: 5569 mean_circ: 0.932772
Ran for 140550 frames Cell Events: 5590 mean_circ: 0.932831
Ran for 142550 frames Cell Events: 5661 mean_circ: 0.932782
Ran for 143550 frames Cell Events: 5691 mean_circ: 0.932794
Ran for 144550 frames Cell Events: 5738 mean_circ: 0.932802
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Ran for 145550 frames Cell Events: 5780 mean_circ: 0.932878
Ran for 146050 frames Cell Events: 5802 mean_circ: 0.932908
Ran for 147050 frames Cell Events: 5844 mean_circ: 0.932838
Ran for 149050 frames Cell Events: 5927 mean_circ: 0.932766
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Ran for 150550 frames Cell Events: 5988 mean_circ: 0.932781
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Ran for 152050 frames Cell Events: 6044 mean_circ: 0.932768
Ran for 152550 frames Cell Events: 6070 mean_circ: 0.932758
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Ran for 156550 frames Cell Events: 6241 mean_circ: 0.932635
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Ran for 157550 frames Cell Events: 6272 mean_circ: 0.932636
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Ran for 168050 frames Cell Events: 6690 mean_circ: 0.932116
Ran for 170550 frames Cell Events: 6772 mean_circ: 0.932085
Ran for 171550 frames Cell Events: 6797 mean_circ: 0.932082
Ran for 172550 frames Cell Events: 6840 mean_circ: 0.932063
Ran for 173050 frames Cell Events: 6863 mean_circ: 0.931986
Ran for 174550 frames Cell Events: 6944 mean_circ: 0.932075
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Ran for 196550 frames Cell Events: 7804 mean_circ: 0.93202
Ran for 198550 frames Cell Events: 7897 mean_circ: 0.931917
Ran for 199550 frames Cell Events: 7937 mean_circ: 0.931947
Ran for 200050 frames Cell Events: 7956 mean_circ: 0.93193
Ran for 200550 frames Cell Events: 7976 mean_circ: 0.931936
End this measurment..
Stopping time: 1.9359e+009
****************************************
Measurement finished
Mean Circularitiy: 0.931944
Mean Area: 657.609
Mean FL-1 intensity: 3782.09
Mean FL-2 intensity: 4241.07
Mean FL-3 intensity: 0
frames processed: 197855
Total Time: 100661ms
Number of Runs (new bg img) 0
Frames collected: 201119
Skipped frames: 106488
Rescued Frames: 1859376
Samples DAQ: 50287145
Samples written: 100559354
Samples per Channel: 50279677
FPS: 1997.99
Samples per second: 499496
Expected samples/s: 500000
Events: 8003
Events per second: 79.5046
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :283126
# 1 frames :103068
# 2 frames :4353
# 3 frames :1603
# 4 frames :751
# 5 frames :2175
# 6 frames :1995
# 7 frames :1361
# 8 frames :830
# 9 frames :530
# 10 frames :494
# 11 frames :357
# 12 frames :266
# 13 frames :217
# 14 frames :183
# 15 frames :152
# 16 frames :120
# 17 frames :91
# 18 frames :82
# 19 frames :66
# 20 frames :62
# 21 frames :55
# 22 frames :31
# 23 frames :33
# 24 frames :31
# 25 frames :29
# 26 frames :13
# 27 frames :15
# 28 frames :12
# 29 frames :9
# 30 frames :4
# 31 frames :7
# 32 frames :4
# 33 frames :8
# 34 frames :4
# 35 frames :4
# 36 frames :2
# 37 frames :3
# 39 frames :2
# 40 frames :2
# 41 frames :1
# 42 frames :3
# 43 frames :2
# 44 frames :4
# 48 frames :1
# 49 frames :1
# 50 frames :1
# 52 frames :1
# 56 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2626
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0