Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.57.08
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "18b7374a5f404edb5deac934c493abd1",
"name": "M1_20170606_1831_dsRNA_LZ_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:57:28"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8136 (max 8137) in '8136'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1352
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.93728e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 89 mean_circ: 0.946166
Ran for 2550 frames Cell Events: 99 mean_circ: 0.946927
Ran for 3050 frames Cell Events: 115 mean_circ: 0.946874
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Ran for 5550 frames Cell Events: 200 mean_circ: 0.948033
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Ran for 77550 frames Cell Events: 2451 mean_circ: 0.950886
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Ran for 86050 frames Cell Events: 2655 mean_circ: 0.950681
Ran for 87050 frames Cell Events: 2688 mean_circ: 0.950697
Ran for 87550 frames Cell Events: 2703 mean_circ: 0.950619
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Ran for 91050 frames Cell Events: 2789 mean_circ: 0.950536
Ran for 93050 frames Cell Events: 2856 mean_circ: 0.95057
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Ran for 94050 frames Cell Events: 2898 mean_circ: 0.95062
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Ran for 95050 frames Cell Events: 2921 mean_circ: 0.950599
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Ran for 96550 frames Cell Events: 2961 mean_circ: 0.950659
Ran for 97050 frames Cell Events: 2980 mean_circ: 0.950729
Ran for 97550 frames Cell Events: 2991 mean_circ: 0.950798
Ran for 98050 frames Cell Events: 3000 mean_circ: 0.950825
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Ran for 121550 frames Cell Events: 3781 mean_circ: 0.951122
Ran for 122050 frames Cell Events: 3796 mean_circ: 0.951162
Ran for 122550 frames Cell Events: 3808 mean_circ: 0.951185
Ran for 123050 frames Cell Events: 3828 mean_circ: 0.951232
Ran for 123550 frames Cell Events: 3846 mean_circ: 0.951204
Ran for 124050 frames Cell Events: 3857 mean_circ: 0.951251
Ran for 124550 frames Cell Events: 3867 mean_circ: 0.951292
Ran for 125050 frames Cell Events: 3883 mean_circ: 0.951318
Ran for 125550 frames Cell Events: 3895 mean_circ: 0.951332
Ran for 126550 frames Cell Events: 3921 mean_circ: 0.9513
Ran for 127050 frames Cell Events: 3935 mean_circ: 0.951289
Ran for 127550 frames Cell Events: 3946 mean_circ: 0.951341
Ran for 128050 frames Cell Events: 3962 mean_circ: 0.951357
Ran for 128550 frames Cell Events: 3985 mean_circ: 0.95138
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Ran for 131050 frames Cell Events: 4069 mean_circ: 0.951496
Ran for 131550 frames Cell Events: 4087 mean_circ: 0.951492
Ran for 132050 frames Cell Events: 4103 mean_circ: 0.951504
Ran for 132550 frames Cell Events: 4120 mean_circ: 0.951512
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Ran for 172550 frames Cell Events: 5423 mean_circ: 0.95099
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Ran for 182050 frames Cell Events: 5721 mean_circ: 0.950951
Ran for 182550 frames Cell Events: 5730 mean_circ: 0.950949
Ran for 183050 frames Cell Events: 5742 mean_circ: 0.950951
Ran for 183550 frames Cell Events: 5754 mean_circ: 0.950977
Ran for 184050 frames Cell Events: 5770 mean_circ: 0.950898
Ran for 184550 frames Cell Events: 5785 mean_circ: 0.95084
Ran for 185050 frames Cell Events: 5797 mean_circ: 0.950816
Ran for 185550 frames Cell Events: 5820 mean_circ: 0.950814
Ran for 186050 frames Cell Events: 5839 mean_circ: 0.950855
Ran for 186550 frames Cell Events: 5851 mean_circ: 0.950816
Ran for 187050 frames Cell Events: 5865 mean_circ: 0.950846
Ran for 187550 frames Cell Events: 5879 mean_circ: 0.950832
Ran for 188050 frames Cell Events: 5897 mean_circ: 0.950846
Ran for 188550 frames Cell Events: 5920 mean_circ: 0.950868
Ran for 189050 frames Cell Events: 5946 mean_circ: 0.950864
Ran for 189550 frames Cell Events: 5951 mean_circ: 0.950849
Ran for 190550 frames Cell Events: 5988 mean_circ: 0.950838
Ran for 191050 frames Cell Events: 6000 mean_circ: 0.950858
Ran for 192550 frames Cell Events: 6049 mean_circ: 0.950858
Ran for 193050 frames Cell Events: 6062 mean_circ: 0.95085
Ran for 194050 frames Cell Events: 6086 mean_circ: 0.950869
Ran for 195550 frames Cell Events: 6144 mean_circ: 0.950798
Ran for 196050 frames Cell Events: 6156 mean_circ: 0.950784
Ran for 196550 frames Cell Events: 6165 mean_circ: 0.950797
Ran for 197050 frames Cell Events: 6184 mean_circ: 0.950813
Ran for 197550 frames Cell Events: 6201 mean_circ: 0.950799
Ran for 198050 frames Cell Events: 6219 mean_circ: 0.950723
Ran for 198550 frames Cell Events: 6239 mean_circ: 0.950714
Ran for 199050 frames Cell Events: 6255 mean_circ: 0.950648
Ran for 199550 frames Cell Events: 6271 mean_circ: 0.950667
Ran for 200050 frames Cell Events: 6284 mean_circ: 0.950665
Ran for 200550 frames Cell Events: 6298 mean_circ: 0.950695
Ran for 201050 frames Cell Events: 6312 mean_circ: 0.950712
Ran for 201550 frames Cell Events: 6332 mean_circ: 0.950734
Ran for 202050 frames Cell Events: 6352 mean_circ: 0.950744
Ran for 202550 frames Cell Events: 6359 mean_circ: 0.950754
Ran for 204050 frames Cell Events: 6402 mean_circ: 0.950744
Ran for 204550 frames Cell Events: 6414 mean_circ: 0.950765
Ran for 206050 frames Cell Events: 6470 mean_circ: 0.950692
Ran for 206550 frames Cell Events: 6490 mean_circ: 0.950691
Ran for 207050 frames Cell Events: 6508 mean_circ: 0.950684
Ran for 207550 frames Cell Events: 6514 mean_circ: 0.950682
Ran for 208050 frames Cell Events: 6533 mean_circ: 0.950694
Ran for 208550 frames Cell Events: 6548 mean_circ: 0.950675
Ran for 209050 frames Cell Events: 6568 mean_circ: 0.950607
Ran for 209550 frames Cell Events: 6584 mean_circ: 0.950659
Ran for 210050 frames Cell Events: 6608 mean_circ: 0.950674
Ran for 211050 frames Cell Events: 6648 mean_circ: 0.950663
Ran for 212050 frames Cell Events: 6682 mean_circ: 0.950647
Ran for 212550 frames Cell Events: 6698 mean_circ: 0.950644
Ran for 213550 frames Cell Events: 6737 mean_circ: 0.950613
Ran for 214050 frames Cell Events: 6751 mean_circ: 0.950642
Ran for 214550 frames Cell Events: 6765 mean_circ: 0.950658
Ran for 215050 frames Cell Events: 6782 mean_circ: 0.950676
Ran for 215550 frames Cell Events: 6793 mean_circ: 0.950666
Ran for 216050 frames Cell Events: 6814 mean_circ: 0.950671
Ran for 216550 frames Cell Events: 6830 mean_circ: 0.950638
Ran for 217050 frames Cell Events: 6850 mean_circ: 0.950649
Ran for 217550 frames Cell Events: 6869 mean_circ: 0.95068
Ran for 218050 frames Cell Events: 6888 mean_circ: 0.950693
Ran for 218550 frames Cell Events: 6905 mean_circ: 0.950699
Ran for 219050 frames Cell Events: 6926 mean_circ: 0.950685
Ran for 219550 frames Cell Events: 6946 mean_circ: 0.950687
Ran for 220050 frames Cell Events: 6958 mean_circ: 0.950698
Ran for 221050 frames Cell Events: 6994 mean_circ: 0.950731
Ran for 223050 frames Cell Events: 7079 mean_circ: 0.950742
Ran for 224050 frames Cell Events: 7111 mean_circ: 0.950742
Ran for 224550 frames Cell Events: 7135 mean_circ: 0.950728
Ran for 225050 frames Cell Events: 7155 mean_circ: 0.950704
Ran for 225550 frames Cell Events: 7172 mean_circ: 0.95069
Ran for 226550 frames Cell Events: 7212 mean_circ: 0.950702
Ran for 227050 frames Cell Events: 7235 mean_circ: 0.950674
Ran for 227550 frames Cell Events: 7260 mean_circ: 0.950653
Ran for 228050 frames Cell Events: 7276 mean_circ: 0.950684
Ran for 230050 frames Cell Events: 7343 mean_circ: 0.950686
Ran for 230550 frames Cell Events: 7355 mean_circ: 0.950698
Ran for 231050 frames Cell Events: 7376 mean_circ: 0.950706
Ran for 232550 frames Cell Events: 7429 mean_circ: 0.950709
Ran for 233550 frames Cell Events: 7462 mean_circ: 0.950711
Ran for 235050 frames Cell Events: 7507 mean_circ: 0.950723
Ran for 235550 frames Cell Events: 7521 mean_circ: 0.950701
Ran for 236050 frames Cell Events: 7535 mean_circ: 0.950724
Ran for 236550 frames Cell Events: 7547 mean_circ: 0.950722
Ran for 237050 frames Cell Events: 7567 mean_circ: 0.950722
Ran for 237550 frames Cell Events: 7575 mean_circ: 0.950733
Ran for 238050 frames Cell Events: 7590 mean_circ: 0.950723
Ran for 238550 frames Cell Events: 7601 mean_circ: 0.950729
Ran for 239050 frames Cell Events: 7617 mean_circ: 0.950746
Ran for 239550 frames Cell Events: 7642 mean_circ: 0.950701
Ran for 240050 frames Cell Events: 7671 mean_circ: 0.950608
Ran for 241050 frames Cell Events: 7708 mean_circ: 0.950627
Ran for 242050 frames Cell Events: 7742 mean_circ: 0.950606
Ran for 243050 frames Cell Events: 7774 mean_circ: 0.950663
Ran for 243550 frames Cell Events: 7793 mean_circ: 0.950684
Ran for 244050 frames Cell Events: 7813 mean_circ: 0.950701
Ran for 244550 frames Cell Events: 7824 mean_circ: 0.95072
Ran for 245050 frames Cell Events: 7837 mean_circ: 0.950737
Ran for 245550 frames Cell Events: 7862 mean_circ: 0.950721
Ran for 246050 frames Cell Events: 7874 mean_circ: 0.950748
Ran for 246550 frames Cell Events: 7885 mean_circ: 0.95074
Ran for 247050 frames Cell Events: 7895 mean_circ: 0.950745
Ran for 247550 frames Cell Events: 7906 mean_circ: 0.95076
Ran for 249050 frames Cell Events: 7951 mean_circ: 0.95076
Ran for 249550 frames Cell Events: 7966 mean_circ: 0.95074
Ran for 250050 frames Cell Events: 7984 mean_circ: 0.950702
Ran for 250550 frames Cell Events: 8002 mean_circ: 0.950683
Ran for 252550 frames Cell Events: 8042 mean_circ: 0.950648
Ran for 254550 frames Cell Events: 8108 mean_circ: 0.950664
End this measurment..
Stopping time: 1.9374e+009
****************************************
Measurement finished
Mean Circularitiy: 0.950663
Mean Area: 655.964
Mean FL-1 intensity: 3994.69
Mean FL-2 intensity: 4687.49
Mean FL-3 intensity: 0
frames processed: 251135
Total Time: 127728ms
Number of Runs (new bg img) 0
Frames collected: 255315
Skipped frames: 114735
Rescued Frames: 2167433
Samples DAQ: 63829004
Samples written: 127657596
Samples per Channel: 63828798
FPS: 1998.9
Samples per second: 499725
Expected samples/s: 500000
Events: 8137
Events per second: 63.7058
jumped back (PMT): 5914
#Frames read, frequency
# 0 frames :342097
# 1 frames :151190
# 2 frames :4880
# 3 frames :1753
# 4 frames :715
# 5 frames :2583
# 6 frames :2122
# 7 frames :1405
# 8 frames :749
# 9 frames :424
# 10 frames :527
# 11 frames :431
# 12 frames :337
# 13 frames :272
# 14 frames :199
# 15 frames :149
# 16 frames :109
# 17 frames :101
# 18 frames :92
# 19 frames :85
# 20 frames :68
# 21 frames :56
# 22 frames :47
# 23 frames :23
# 24 frames :23
# 25 frames :20
# 26 frames :24
# 27 frames :16
# 28 frames :6
# 29 frames :6
# 30 frames :3
# 31 frames :6
# 32 frames :6
# 33 frames :2
# 34 frames :2
# 36 frames :1
# 37 frames :1
# 38 frames :3
# 44 frames :1
# 50 frames :1
# 58 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1352
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0