Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.58.33
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "989d50da28a4358dbd0af3a80fa02f4c",
"name": "M1_20170606_1853_dsRNA_LZ_repeat_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:58:53"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7978 (max 7979) in '7978'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.184
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.93862e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 42 mean_circ: 0.925994
Ran for 1550 frames Cell Events: 64 mean_circ: 0.931839
Ran for 2050 frames Cell Events: 82 mean_circ: 0.934615
Ran for 3550 frames Cell Events: 138 mean_circ: 0.938387
Ran for 5550 frames Cell Events: 262 mean_circ: 0.939514
Ran for 7050 frames Cell Events: 334 mean_circ: 0.940096
Ran for 8550 frames Cell Events: 394 mean_circ: 0.940571
Ran for 9050 frames Cell Events: 416 mean_circ: 0.939439
Ran for 9550 frames Cell Events: 435 mean_circ: 0.940128
Ran for 11050 frames Cell Events: 502 mean_circ: 0.939619
Ran for 12550 frames Cell Events: 562 mean_circ: 0.940421
Ran for 13050 frames Cell Events: 587 mean_circ: 0.940388
Ran for 13550 frames Cell Events: 609 mean_circ: 0.940321
Ran for 14050 frames Cell Events: 634 mean_circ: 0.940625
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Ran for 17050 frames Cell Events: 752 mean_circ: 0.940925
Ran for 18050 frames Cell Events: 794 mean_circ: 0.941304
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Ran for 19550 frames Cell Events: 833 mean_circ: 0.940368
Ran for 20550 frames Cell Events: 865 mean_circ: 0.940081
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Ran for 66550 frames Cell Events: 2803 mean_circ: 0.939106
Ran for 67050 frames Cell Events: 2825 mean_circ: 0.939038
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Ran for 70050 frames Cell Events: 2943 mean_circ: 0.939155
Ran for 71050 frames Cell Events: 2975 mean_circ: 0.9392
Ran for 72050 frames Cell Events: 3008 mean_circ: 0.939318
Ran for 72550 frames Cell Events: 3031 mean_circ: 0.939415
Ran for 73550 frames Cell Events: 3086 mean_circ: 0.939331
Ran for 74550 frames Cell Events: 3118 mean_circ: 0.939333
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Ran for 75550 frames Cell Events: 3153 mean_circ: 0.939283
Ran for 76050 frames Cell Events: 3178 mean_circ: 0.939267
Ran for 76550 frames Cell Events: 3193 mean_circ: 0.939347
Ran for 77550 frames Cell Events: 3235 mean_circ: 0.939324
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Ran for 78550 frames Cell Events: 3281 mean_circ: 0.939255
Ran for 79050 frames Cell Events: 3299 mean_circ: 0.939306
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Ran for 80550 frames Cell Events: 3360 mean_circ: 0.9394
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Ran for 81550 frames Cell Events: 3404 mean_circ: 0.939571
Ran for 82050 frames Cell Events: 3426 mean_circ: 0.939587
Ran for 82550 frames Cell Events: 3446 mean_circ: 0.939522
Ran for 83050 frames Cell Events: 3472 mean_circ: 0.939474
Ran for 83550 frames Cell Events: 3486 mean_circ: 0.939471
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Ran for 85050 frames Cell Events: 3547 mean_circ: 0.939334
Ran for 85550 frames Cell Events: 3572 mean_circ: 0.939274
Ran for 86550 frames Cell Events: 3631 mean_circ: 0.939069
Ran for 87550 frames Cell Events: 3683 mean_circ: 0.939125
Ran for 88050 frames Cell Events: 3705 mean_circ: 0.939114
Ran for 88550 frames Cell Events: 3727 mean_circ: 0.939025
Ran for 90550 frames Cell Events: 3810 mean_circ: 0.938952
Ran for 91050 frames Cell Events: 3836 mean_circ: 0.938936
Ran for 92550 frames Cell Events: 3902 mean_circ: 0.939075
Ran for 93050 frames Cell Events: 3913 mean_circ: 0.939109
Ran for 93550 frames Cell Events: 3940 mean_circ: 0.939107
Ran for 94550 frames Cell Events: 3970 mean_circ: 0.939101
Ran for 96050 frames Cell Events: 4035 mean_circ: 0.939133
Ran for 96550 frames Cell Events: 4051 mean_circ: 0.939174
Ran for 97050 frames Cell Events: 4069 mean_circ: 0.939148
Ran for 98050 frames Cell Events: 4115 mean_circ: 0.939057
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Ran for 100050 frames Cell Events: 4217 mean_circ: 0.93917
Ran for 100550 frames Cell Events: 4242 mean_circ: 0.939133
Ran for 101050 frames Cell Events: 4261 mean_circ: 0.939179
Ran for 101550 frames Cell Events: 4284 mean_circ: 0.939206
Ran for 102050 frames Cell Events: 4301 mean_circ: 0.939206
Ran for 102550 frames Cell Events: 4325 mean_circ: 0.939116
Ran for 103550 frames Cell Events: 4364 mean_circ: 0.939146
Ran for 104050 frames Cell Events: 4384 mean_circ: 0.939181
Ran for 104550 frames Cell Events: 4413 mean_circ: 0.939154
Ran for 105050 frames Cell Events: 4442 mean_circ: 0.93904
Ran for 106050 frames Cell Events: 4496 mean_circ: 0.939174
Ran for 106550 frames Cell Events: 4517 mean_circ: 0.939134
Ran for 107050 frames Cell Events: 4531 mean_circ: 0.939147
Ran for 107550 frames Cell Events: 4548 mean_circ: 0.939152
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Ran for 110050 frames Cell Events: 4633 mean_circ: 0.93909
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Ran for 115050 frames Cell Events: 4854 mean_circ: 0.939139
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Ran for 120050 frames Cell Events: 5087 mean_circ: 0.939174
Ran for 120550 frames Cell Events: 5101 mean_circ: 0.939223
Ran for 121050 frames Cell Events: 5117 mean_circ: 0.939226
Ran for 121550 frames Cell Events: 5140 mean_circ: 0.939251
Ran for 122050 frames Cell Events: 5152 mean_circ: 0.939191
Ran for 122550 frames Cell Events: 5175 mean_circ: 0.939201
Ran for 123550 frames Cell Events: 5225 mean_circ: 0.939178
Ran for 124050 frames Cell Events: 5245 mean_circ: 0.9391
Ran for 125050 frames Cell Events: 5290 mean_circ: 0.939203
Ran for 125550 frames Cell Events: 5310 mean_circ: 0.939264
Ran for 126050 frames Cell Events: 5341 mean_circ: 0.939306
Ran for 126550 frames Cell Events: 5358 mean_circ: 0.939319
Ran for 127550 frames Cell Events: 5393 mean_circ: 0.939412
Ran for 128050 frames Cell Events: 5414 mean_circ: 0.939425
Ran for 128550 frames Cell Events: 5446 mean_circ: 0.939443
Ran for 129050 frames Cell Events: 5457 mean_circ: 0.939342
Ran for 129550 frames Cell Events: 5473 mean_circ: 0.939387
Ran for 130050 frames Cell Events: 5494 mean_circ: 0.93935
Ran for 130550 frames Cell Events: 5515 mean_circ: 0.939401
Ran for 131050 frames Cell Events: 5536 mean_circ: 0.939408
Ran for 132050 frames Cell Events: 5567 mean_circ: 0.939424
Ran for 132550 frames Cell Events: 5591 mean_circ: 0.939448
Ran for 133050 frames Cell Events: 5615 mean_circ: 0.939347
Ran for 133550 frames Cell Events: 5632 mean_circ: 0.939305
Ran for 134550 frames Cell Events: 5686 mean_circ: 0.939319
Ran for 135550 frames Cell Events: 5727 mean_circ: 0.939349
Ran for 136050 frames Cell Events: 5743 mean_circ: 0.939293
Ran for 136550 frames Cell Events: 5772 mean_circ: 0.939261
Ran for 137050 frames Cell Events: 5795 mean_circ: 0.939273
Ran for 137550 frames Cell Events: 5815 mean_circ: 0.939292
Ran for 138050 frames Cell Events: 5834 mean_circ: 0.939216
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Ran for 140550 frames Cell Events: 5934 mean_circ: 0.939274
Ran for 141050 frames Cell Events: 5954 mean_circ: 0.939257
Ran for 141550 frames Cell Events: 5975 mean_circ: 0.939294
Ran for 142550 frames Cell Events: 6019 mean_circ: 0.939283
Ran for 143050 frames Cell Events: 6047 mean_circ: 0.939251
Ran for 143550 frames Cell Events: 6072 mean_circ: 0.939258
Ran for 144050 frames Cell Events: 6093 mean_circ: 0.93921
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Ran for 145550 frames Cell Events: 6153 mean_circ: 0.939203
Ran for 146050 frames Cell Events: 6178 mean_circ: 0.9392
Ran for 147050 frames Cell Events: 6214 mean_circ: 0.939222
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Ran for 151050 frames Cell Events: 6341 mean_circ: 0.939183
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Ran for 152550 frames Cell Events: 6414 mean_circ: 0.9393
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Ran for 167050 frames Cell Events: 7015 mean_circ: 0.939583
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Ran for 172550 frames Cell Events: 7246 mean_circ: 0.939649
Ran for 173050 frames Cell Events: 7263 mean_circ: 0.939597
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Ran for 182550 frames Cell Events: 7653 mean_circ: 0.939432
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Ran for 184550 frames Cell Events: 7722 mean_circ: 0.939364
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Ran for 186550 frames Cell Events: 7814 mean_circ: 0.939286
Ran for 187050 frames Cell Events: 7829 mean_circ: 0.939313
Ran for 187550 frames Cell Events: 7849 mean_circ: 0.939329
Ran for 188050 frames Cell Events: 7864 mean_circ: 0.939329
Ran for 188550 frames Cell Events: 7880 mean_circ: 0.939373
Ran for 189550 frames Cell Events: 7906 mean_circ: 0.939379
Ran for 190550 frames Cell Events: 7942 mean_circ: 0.939359
End this measurment..
Stopping time: 1.93871e+009
****************************************
Measurement finished
Mean Circularitiy: 0.939375
Mean Area: 643.359
Mean FL-1 intensity: 3978.67
Mean FL-2 intensity: 5722.92
Mean FL-3 intensity: 0
frames processed: 190328
Total Time: 95816.7ms
Number of Runs (new bg img) 0
Frames collected: 191489
Skipped frames: 120213
Rescued Frames: 2392281
Samples DAQ: 47872402
Samples written: 95744534
Samples per Channel: 47872267
FPS: 1998.49
Samples per second: 499624
Expected samples/s: 500000
Events: 7979
Events per second: 83.2736
jumped back (PMT): 13
#Frames read, frequency
# 0 frames :259049
# 1 frames :109540
# 2 frames :3636
# 3 frames :1281
# 4 frames :474
# 5 frames :3053
# 6 frames :2059
# 7 frames :1145
# 8 frames :578
# 9 frames :386
# 10 frames :422
# 11 frames :294
# 12 frames :222
# 13 frames :164
# 14 frames :132
# 15 frames :94
# 16 frames :72
# 17 frames :69
# 18 frames :49
# 19 frames :58
# 20 frames :31
# 21 frames :32
# 22 frames :24
# 23 frames :22
# 24 frames :12
# 25 frames :10
# 26 frames :7
# 27 frames :10
# 28 frames :4
# 29 frames :7
# 30 frames :5
# 31 frames :2
# 32 frames :2
# 33 frames :2
# 36 frames :1
# 39 frames :1
# 42 frames :1
# 44 frames :1
# 47 frames :2
# 89 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.184
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0