Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.12.21
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "12e88e07b40973e1967741f4942bea6c",
"name": "M1_20170606_2158_dsRNA_Q6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:12:41"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8086 (max 8087) in '8086'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1904
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.94975e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 73 mean_circ: 0.944107
Ran for 1550 frames Cell Events: 101 mean_circ: 0.943112
Ran for 6050 frames Cell Events: 377 mean_circ: 0.939841
Ran for 7050 frames Cell Events: 451 mean_circ: 0.939266
Ran for 7550 frames Cell Events: 484 mean_circ: 0.939543
Ran for 8050 frames Cell Events: 529 mean_circ: 0.940843
Ran for 9550 frames Cell Events: 615 mean_circ: 0.940862
Ran for 10050 frames Cell Events: 643 mean_circ: 0.940271
Ran for 10550 frames Cell Events: 664 mean_circ: 0.939898
Ran for 11050 frames Cell Events: 689 mean_circ: 0.939577
Ran for 12050 frames Cell Events: 768 mean_circ: 0.939077
Ran for 13050 frames Cell Events: 819 mean_circ: 0.938741
Ran for 14050 frames Cell Events: 883 mean_circ: 0.939412
Ran for 15050 frames Cell Events: 956 mean_circ: 0.939359
Ran for 16050 frames Cell Events: 1032 mean_circ: 0.93926
Ran for 16550 frames Cell Events: 1062 mean_circ: 0.938903
Ran for 17050 frames Cell Events: 1094 mean_circ: 0.938986
Ran for 17550 frames Cell Events: 1119 mean_circ: 0.93916
Ran for 18550 frames Cell Events: 1178 mean_circ: 0.939021
Ran for 19050 frames Cell Events: 1211 mean_circ: 0.938722
Ran for 21050 frames Cell Events: 1382 mean_circ: 0.938274
Ran for 22050 frames Cell Events: 1479 mean_circ: 0.93807
Ran for 22550 frames Cell Events: 1511 mean_circ: 0.937877
Ran for 23050 frames Cell Events: 1554 mean_circ: 0.937454
Ran for 24050 frames Cell Events: 1632 mean_circ: 0.937766
Ran for 26050 frames Cell Events: 1768 mean_circ: 0.938026
Ran for 26550 frames Cell Events: 1797 mean_circ: 0.938149
Ran for 27050 frames Cell Events: 1826 mean_circ: 0.938289
Ran for 27550 frames Cell Events: 1849 mean_circ: 0.938296
Ran for 28050 frames Cell Events: 1870 mean_circ: 0.938271
Ran for 28550 frames Cell Events: 1897 mean_circ: 0.938461
Ran for 29050 frames Cell Events: 1922 mean_circ: 0.938536
Ran for 29550 frames Cell Events: 1946 mean_circ: 0.938664
Ran for 30550 frames Cell Events: 2006 mean_circ: 0.938834
Ran for 31550 frames Cell Events: 2078 mean_circ: 0.9389
Ran for 32050 frames Cell Events: 2109 mean_circ: 0.939015
Ran for 33050 frames Cell Events: 2154 mean_circ: 0.939067
Ran for 33550 frames Cell Events: 2202 mean_circ: 0.939108
Ran for 34050 frames Cell Events: 2230 mean_circ: 0.939329
Ran for 34550 frames Cell Events: 2251 mean_circ: 0.939277
Ran for 35050 frames Cell Events: 2275 mean_circ: 0.939388
Ran for 36050 frames Cell Events: 2338 mean_circ: 0.939406
Ran for 36550 frames Cell Events: 2364 mean_circ: 0.939511
Ran for 37050 frames Cell Events: 2396 mean_circ: 0.939597
Ran for 38550 frames Cell Events: 2480 mean_circ: 0.93967
Ran for 39050 frames Cell Events: 2501 mean_circ: 0.939786
Ran for 40050 frames Cell Events: 2555 mean_circ: 0.939893
Ran for 40550 frames Cell Events: 2592 mean_circ: 0.93987
Ran for 41050 frames Cell Events: 2626 mean_circ: 0.939809
Ran for 43050 frames Cell Events: 2735 mean_circ: 0.939745
Ran for 44050 frames Cell Events: 2794 mean_circ: 0.939774
Ran for 44550 frames Cell Events: 2831 mean_circ: 0.939861
Ran for 45050 frames Cell Events: 2860 mean_circ: 0.939877
Ran for 46550 frames Cell Events: 2997 mean_circ: 0.940005
Ran for 47050 frames Cell Events: 3034 mean_circ: 0.940023
Ran for 48050 frames Cell Events: 3085 mean_circ: 0.939931
Ran for 49050 frames Cell Events: 3137 mean_circ: 0.940076
Ran for 49550 frames Cell Events: 3156 mean_circ: 0.940125
Ran for 50050 frames Cell Events: 3185 mean_circ: 0.940021
Ran for 51050 frames Cell Events: 3237 mean_circ: 0.940095
Ran for 52050 frames Cell Events: 3286 mean_circ: 0.939993
Ran for 52550 frames Cell Events: 3316 mean_circ: 0.939987
Ran for 53050 frames Cell Events: 3348 mean_circ: 0.94
Ran for 53550 frames Cell Events: 3384 mean_circ: 0.93999
Ran for 55050 frames Cell Events: 3473 mean_circ: 0.939959
Ran for 55550 frames Cell Events: 3507 mean_circ: 0.939993
Ran for 56050 frames Cell Events: 3531 mean_circ: 0.94001
Ran for 56550 frames Cell Events: 3557 mean_circ: 0.940009
Ran for 57550 frames Cell Events: 3627 mean_circ: 0.93997
Ran for 58050 frames Cell Events: 3651 mean_circ: 0.940026
Ran for 58550 frames Cell Events: 3681 mean_circ: 0.940099
Ran for 59050 frames Cell Events: 3708 mean_circ: 0.940071
Ran for 60550 frames Cell Events: 3792 mean_circ: 0.940014
Ran for 61050 frames Cell Events: 3823 mean_circ: 0.940023
Ran for 62050 frames Cell Events: 3886 mean_circ: 0.939974
Ran for 62550 frames Cell Events: 3929 mean_circ: 0.939951
Ran for 63050 frames Cell Events: 3953 mean_circ: 0.939998
Ran for 63550 frames Cell Events: 3982 mean_circ: 0.939976
Ran for 64050 frames Cell Events: 4011 mean_circ: 0.939994
Ran for 64550 frames Cell Events: 4036 mean_circ: 0.940038
Ran for 65050 frames Cell Events: 4059 mean_circ: 0.940053
Ran for 66050 frames Cell Events: 4105 mean_circ: 0.940051
Ran for 66550 frames Cell Events: 4129 mean_circ: 0.939988
Ran for 68550 frames Cell Events: 4258 mean_circ: 0.94005
Ran for 69050 frames Cell Events: 4281 mean_circ: 0.940072
Ran for 69550 frames Cell Events: 4303 mean_circ: 0.939949
Ran for 70550 frames Cell Events: 4357 mean_circ: 0.939809
Ran for 73550 frames Cell Events: 4504 mean_circ: 0.939846
Ran for 75550 frames Cell Events: 4611 mean_circ: 0.939997
Ran for 78050 frames Cell Events: 4786 mean_circ: 0.940002
Ran for 78550 frames Cell Events: 4818 mean_circ: 0.940038
Ran for 79050 frames Cell Events: 4846 mean_circ: 0.939973
Ran for 79550 frames Cell Events: 4886 mean_circ: 0.939898
Ran for 81550 frames Cell Events: 5003 mean_circ: 0.939966
Ran for 82050 frames Cell Events: 5023 mean_circ: 0.939967
Ran for 83050 frames Cell Events: 5095 mean_circ: 0.939863
Ran for 83550 frames Cell Events: 5127 mean_circ: 0.939861
Ran for 84050 frames Cell Events: 5145 mean_circ: 0.939919
Ran for 84550 frames Cell Events: 5172 mean_circ: 0.939924
Ran for 85050 frames Cell Events: 5196 mean_circ: 0.93992
Ran for 85550 frames Cell Events: 5224 mean_circ: 0.939939
Ran for 86550 frames Cell Events: 5281 mean_circ: 0.940004
Ran for 87550 frames Cell Events: 5324 mean_circ: 0.940064
Ran for 89050 frames Cell Events: 5405 mean_circ: 0.940026
Ran for 89550 frames Cell Events: 5427 mean_circ: 0.940065
Ran for 91550 frames Cell Events: 5527 mean_circ: 0.94001
Ran for 92050 frames Cell Events: 5554 mean_circ: 0.939996
Ran for 93050 frames Cell Events: 5616 mean_circ: 0.939831
Ran for 96050 frames Cell Events: 5786 mean_circ: 0.939855
Ran for 97050 frames Cell Events: 5853 mean_circ: 0.939951
Ran for 98050 frames Cell Events: 5912 mean_circ: 0.940008
Ran for 98550 frames Cell Events: 5936 mean_circ: 0.940011
Ran for 99050 frames Cell Events: 5964 mean_circ: 0.939959
Ran for 100050 frames Cell Events: 6010 mean_circ: 0.939983
Ran for 101050 frames Cell Events: 6058 mean_circ: 0.939992
Ran for 101550 frames Cell Events: 6083 mean_circ: 0.939941
Ran for 102550 frames Cell Events: 6159 mean_circ: 0.939862
Ran for 103550 frames Cell Events: 6218 mean_circ: 0.9399
Ran for 104050 frames Cell Events: 6256 mean_circ: 0.939909
Ran for 104550 frames Cell Events: 6286 mean_circ: 0.939903
Ran for 105550 frames Cell Events: 6353 mean_circ: 0.939901
Ran for 106050 frames Cell Events: 6381 mean_circ: 0.939877
Ran for 106550 frames Cell Events: 6412 mean_circ: 0.939806
Ran for 107050 frames Cell Events: 6446 mean_circ: 0.939859
Ran for 108050 frames Cell Events: 6515 mean_circ: 0.939871
Ran for 108550 frames Cell Events: 6552 mean_circ: 0.939876
Ran for 109050 frames Cell Events: 6597 mean_circ: 0.939842
Ran for 111050 frames Cell Events: 6741 mean_circ: 0.93975
Ran for 112050 frames Cell Events: 6797 mean_circ: 0.939787
Ran for 112550 frames Cell Events: 6830 mean_circ: 0.939724
Ran for 113050 frames Cell Events: 6878 mean_circ: 0.939757
Ran for 114050 frames Cell Events: 6930 mean_circ: 0.939678
Ran for 114550 frames Cell Events: 6960 mean_circ: 0.939705
Ran for 115550 frames Cell Events: 7015 mean_circ: 0.939688
Ran for 116050 frames Cell Events: 7050 mean_circ: 0.939661
Ran for 117050 frames Cell Events: 7117 mean_circ: 0.939716
Ran for 119050 frames Cell Events: 7256 mean_circ: 0.939711
Ran for 119550 frames Cell Events: 7283 mean_circ: 0.939696
Ran for 120550 frames Cell Events: 7348 mean_circ: 0.939761
Ran for 123050 frames Cell Events: 7505 mean_circ: 0.93972
Ran for 124050 frames Cell Events: 7556 mean_circ: 0.939765
Ran for 127050 frames Cell Events: 7754 mean_circ: 0.939752
Ran for 128550 frames Cell Events: 7857 mean_circ: 0.939721
Ran for 129050 frames Cell Events: 7884 mean_circ: 0.939662
Ran for 129550 frames Cell Events: 7912 mean_circ: 0.939691
Ran for 130550 frames Cell Events: 7999 mean_circ: 0.93965
Ran for 131550 frames Cell Events: 8063 mean_circ: 0.939566
End this measurment..
Stopping time: 1.94982e+009
****************************************
Measurement finished
Mean Circularitiy: 0.939572
Mean Area: 634.234
Mean FL-1 intensity: 4129.72
Mean FL-2 intensity: 5490.01
Mean FL-3 intensity: 0
frames processed: 130632
Total Time: 66009.4ms
Number of Runs (new bg img) 0
Frames collected: 131870
Skipped frames: 165029
Rescued Frames: 5154370
Samples DAQ: 32967403
Samples written: 65934708
Samples per Channel: 32967354
FPS: 1997.75
Samples per second: 499434
Expected samples/s: 500000
Events: 8087
Events per second: 122.513
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :195728
# 1 frames :56194
# 2 frames :2554
# 3 frames :1031
# 4 frames :378
# 5 frames :2366
# 6 frames :1497
# 7 frames :998
# 8 frames :535
# 9 frames :309
# 10 frames :398
# 11 frames :282
# 12 frames :261
# 13 frames :192
# 14 frames :149
# 15 frames :107
# 16 frames :78
# 17 frames :98
# 18 frames :81
# 19 frames :81
# 20 frames :40
# 21 frames :39
# 22 frames :38
# 23 frames :31
# 24 frames :23
# 25 frames :23
# 26 frames :15
# 27 frames :16
# 28 frames :13
# 29 frames :8
# 30 frames :6
# 31 frames :4
# 32 frames :6
# 33 frames :5
# 34 frames :4
# 35 frames :7
# 36 frames :2
# 37 frames :4
# 38 frames :4
# 39 frames :3
# 40 frames :1
# 42 frames :1
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 47 frames :1
# 49 frames :1
# 84 frames :1
# 95 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1904
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0