Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.12.48
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7af905f5551712aa2163ba8051818b48",
"name": "M1_20170606_2206_dsRNA_Q7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:13:08"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8196 (max 8198) in '8196'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.24
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.95023e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 64 mean_circ: 0.939655
Ran for 2550 frames Cell Events: 145 mean_circ: 0.941014
Ran for 3550 frames Cell Events: 202 mean_circ: 0.944494
Ran for 6050 frames Cell Events: 356 mean_circ: 0.945096
Ran for 7550 frames Cell Events: 402 mean_circ: 0.945995
Ran for 8050 frames Cell Events: 431 mean_circ: 0.945764
Ran for 8550 frames Cell Events: 455 mean_circ: 0.945889
Ran for 9050 frames Cell Events: 487 mean_circ: 0.94589
Ran for 10550 frames Cell Events: 571 mean_circ: 0.946494
Ran for 11050 frames Cell Events: 596 mean_circ: 0.946396
Ran for 12050 frames Cell Events: 633 mean_circ: 0.946456
Ran for 12550 frames Cell Events: 657 mean_circ: 0.946749
Ran for 13550 frames Cell Events: 705 mean_circ: 0.946608
Ran for 14050 frames Cell Events: 736 mean_circ: 0.947166
Ran for 14550 frames Cell Events: 762 mean_circ: 0.94742
Ran for 17050 frames Cell Events: 918 mean_circ: 0.947788
Ran for 17550 frames Cell Events: 956 mean_circ: 0.947539
Ran for 18050 frames Cell Events: 989 mean_circ: 0.947198
Ran for 18550 frames Cell Events: 1028 mean_circ: 0.94721
Ran for 19050 frames Cell Events: 1059 mean_circ: 0.947404
Ran for 19550 frames Cell Events: 1087 mean_circ: 0.947254
Ran for 20050 frames Cell Events: 1109 mean_circ: 0.947552
Ran for 20550 frames Cell Events: 1127 mean_circ: 0.947676
Ran for 21050 frames Cell Events: 1161 mean_circ: 0.947829
Ran for 22050 frames Cell Events: 1208 mean_circ: 0.947635
Ran for 23550 frames Cell Events: 1304 mean_circ: 0.947379
Ran for 26050 frames Cell Events: 1441 mean_circ: 0.948041
Ran for 28050 frames Cell Events: 1596 mean_circ: 0.947543
Ran for 28550 frames Cell Events: 1635 mean_circ: 0.947785
Ran for 29050 frames Cell Events: 1662 mean_circ: 0.947646
Ran for 29550 frames Cell Events: 1681 mean_circ: 0.947669
Ran for 30050 frames Cell Events: 1703 mean_circ: 0.947876
Ran for 31050 frames Cell Events: 1773 mean_circ: 0.947927
Ran for 31550 frames Cell Events: 1805 mean_circ: 0.947593
Ran for 32550 frames Cell Events: 1865 mean_circ: 0.947762
Ran for 33050 frames Cell Events: 1906 mean_circ: 0.94789
Ran for 33550 frames Cell Events: 1938 mean_circ: 0.947673
Ran for 34050 frames Cell Events: 1969 mean_circ: 0.947661
Ran for 34550 frames Cell Events: 1997 mean_circ: 0.947724
Ran for 35550 frames Cell Events: 2066 mean_circ: 0.947908
Ran for 36550 frames Cell Events: 2116 mean_circ: 0.947852
Ran for 37050 frames Cell Events: 2142 mean_circ: 0.947851
Ran for 37550 frames Cell Events: 2172 mean_circ: 0.947944
Ran for 38550 frames Cell Events: 2230 mean_circ: 0.947945
Ran for 39550 frames Cell Events: 2288 mean_circ: 0.947972
Ran for 40050 frames Cell Events: 2317 mean_circ: 0.948065
Ran for 41050 frames Cell Events: 2367 mean_circ: 0.948065
Ran for 41550 frames Cell Events: 2392 mean_circ: 0.948067
Ran for 42050 frames Cell Events: 2411 mean_circ: 0.948176
Ran for 43550 frames Cell Events: 2485 mean_circ: 0.948311
Ran for 44050 frames Cell Events: 2511 mean_circ: 0.948313
Ran for 45550 frames Cell Events: 2615 mean_circ: 0.948603
Ran for 46550 frames Cell Events: 2670 mean_circ: 0.948542
Ran for 47050 frames Cell Events: 2699 mean_circ: 0.948567
Ran for 48050 frames Cell Events: 2760 mean_circ: 0.948672
Ran for 49550 frames Cell Events: 2840 mean_circ: 0.948417
Ran for 50050 frames Cell Events: 2849 mean_circ: 0.948367
Ran for 50550 frames Cell Events: 2861 mean_circ: 0.948389
Ran for 51550 frames Cell Events: 2909 mean_circ: 0.948387
Ran for 52050 frames Cell Events: 2940 mean_circ: 0.948303
Ran for 53050 frames Cell Events: 3011 mean_circ: 0.948181
Ran for 53550 frames Cell Events: 3047 mean_circ: 0.948114
Ran for 54050 frames Cell Events: 3082 mean_circ: 0.948143
Ran for 55050 frames Cell Events: 3148 mean_circ: 0.948243
Ran for 55550 frames Cell Events: 3176 mean_circ: 0.948217
Ran for 56550 frames Cell Events: 3232 mean_circ: 0.948261
Ran for 57050 frames Cell Events: 3254 mean_circ: 0.948356
Ran for 58550 frames Cell Events: 3341 mean_circ: 0.948429
Ran for 59050 frames Cell Events: 3368 mean_circ: 0.948355
Ran for 60050 frames Cell Events: 3422 mean_circ: 0.94829
Ran for 61550 frames Cell Events: 3517 mean_circ: 0.94828
Ran for 62050 frames Cell Events: 3543 mean_circ: 0.94823
Ran for 63050 frames Cell Events: 3591 mean_circ: 0.948184
Ran for 63550 frames Cell Events: 3621 mean_circ: 0.948204
Ran for 64550 frames Cell Events: 3674 mean_circ: 0.948351
Ran for 65050 frames Cell Events: 3704 mean_circ: 0.948354
Ran for 65550 frames Cell Events: 3737 mean_circ: 0.948319
Ran for 66550 frames Cell Events: 3804 mean_circ: 0.94827
Ran for 69050 frames Cell Events: 3950 mean_circ: 0.948156
Ran for 69550 frames Cell Events: 3966 mean_circ: 0.948222
Ran for 70550 frames Cell Events: 4041 mean_circ: 0.94804
Ran for 71050 frames Cell Events: 4072 mean_circ: 0.947988
Ran for 72050 frames Cell Events: 4123 mean_circ: 0.947972
Ran for 72550 frames Cell Events: 4146 mean_circ: 0.948006
Ran for 75050 frames Cell Events: 4298 mean_circ: 0.947878
Ran for 76050 frames Cell Events: 4351 mean_circ: 0.947875
Ran for 76550 frames Cell Events: 4366 mean_circ: 0.947904
Ran for 77050 frames Cell Events: 4392 mean_circ: 0.947881
Ran for 77550 frames Cell Events: 4428 mean_circ: 0.94783
Ran for 78550 frames Cell Events: 4470 mean_circ: 0.947854
Ran for 79550 frames Cell Events: 4524 mean_circ: 0.947917
Ran for 80050 frames Cell Events: 4550 mean_circ: 0.947963
Ran for 81050 frames Cell Events: 4582 mean_circ: 0.94791
Ran for 81550 frames Cell Events: 4605 mean_circ: 0.94785
Ran for 82050 frames Cell Events: 4625 mean_circ: 0.947838
Ran for 82550 frames Cell Events: 4637 mean_circ: 0.947845
Ran for 83550 frames Cell Events: 4687 mean_circ: 0.947901
Ran for 84050 frames Cell Events: 4722 mean_circ: 0.947907
Ran for 85050 frames Cell Events: 4778 mean_circ: 0.947869
Ran for 85550 frames Cell Events: 4801 mean_circ: 0.947926
Ran for 87550 frames Cell Events: 4926 mean_circ: 0.947876
Ran for 88550 frames Cell Events: 4978 mean_circ: 0.947914
Ran for 89050 frames Cell Events: 5003 mean_circ: 0.947981
Ran for 89550 frames Cell Events: 5030 mean_circ: 0.947976
Ran for 90550 frames Cell Events: 5102 mean_circ: 0.948062
Ran for 91050 frames Cell Events: 5135 mean_circ: 0.947984
Ran for 91550 frames Cell Events: 5160 mean_circ: 0.948032
Ran for 92050 frames Cell Events: 5183 mean_circ: 0.948094
Ran for 92550 frames Cell Events: 5227 mean_circ: 0.948
Ran for 93050 frames Cell Events: 5249 mean_circ: 0.94803
Ran for 93550 frames Cell Events: 5269 mean_circ: 0.948036
Ran for 95050 frames Cell Events: 5365 mean_circ: 0.948063
Ran for 97050 frames Cell Events: 5504 mean_circ: 0.947897
Ran for 97550 frames Cell Events: 5527 mean_circ: 0.947964
Ran for 98550 frames Cell Events: 5567 mean_circ: 0.948006
Ran for 99050 frames Cell Events: 5596 mean_circ: 0.947987
Ran for 100550 frames Cell Events: 5677 mean_circ: 0.948023
Ran for 101050 frames Cell Events: 5709 mean_circ: 0.948029
Ran for 101550 frames Cell Events: 5726 mean_circ: 0.948013
Ran for 102050 frames Cell Events: 5754 mean_circ: 0.94803
Ran for 102550 frames Cell Events: 5783 mean_circ: 0.948075
Ran for 103050 frames Cell Events: 5823 mean_circ: 0.948082
Ran for 104050 frames Cell Events: 5866 mean_circ: 0.948108
Ran for 104550 frames Cell Events: 5897 mean_circ: 0.94809
Ran for 105050 frames Cell Events: 5929 mean_circ: 0.947975
Ran for 107050 frames Cell Events: 6030 mean_circ: 0.947989
Ran for 107550 frames Cell Events: 6055 mean_circ: 0.948002
Ran for 108050 frames Cell Events: 6094 mean_circ: 0.948033
Ran for 109050 frames Cell Events: 6133 mean_circ: 0.948035
Ran for 109550 frames Cell Events: 6160 mean_circ: 0.948065
Ran for 110050 frames Cell Events: 6192 mean_circ: 0.948114
Ran for 111050 frames Cell Events: 6247 mean_circ: 0.948154
Ran for 111550 frames Cell Events: 6263 mean_circ: 0.948146
Ran for 113050 frames Cell Events: 6349 mean_circ: 0.948039
Ran for 113550 frames Cell Events: 6372 mean_circ: 0.948041
Ran for 114050 frames Cell Events: 6386 mean_circ: 0.94802
Ran for 114550 frames Cell Events: 6407 mean_circ: 0.94805
Ran for 115050 frames Cell Events: 6441 mean_circ: 0.947983
Ran for 115550 frames Cell Events: 6464 mean_circ: 0.947995
Ran for 116550 frames Cell Events: 6491 mean_circ: 0.948002
Ran for 117550 frames Cell Events: 6546 mean_circ: 0.948096
Ran for 118550 frames Cell Events: 6611 mean_circ: 0.948127
Ran for 120050 frames Cell Events: 6686 mean_circ: 0.948122
Ran for 120550 frames Cell Events: 6721 mean_circ: 0.94816
Ran for 121550 frames Cell Events: 6784 mean_circ: 0.948137
Ran for 122050 frames Cell Events: 6812 mean_circ: 0.948178
Ran for 123550 frames Cell Events: 6886 mean_circ: 0.948175
Ran for 125550 frames Cell Events: 7032 mean_circ: 0.948048
Ran for 126050 frames Cell Events: 7061 mean_circ: 0.948058
Ran for 126550 frames Cell Events: 7093 mean_circ: 0.948021
Ran for 128050 frames Cell Events: 7189 mean_circ: 0.948002
Ran for 129550 frames Cell Events: 7285 mean_circ: 0.948007
Ran for 131550 frames Cell Events: 7386 mean_circ: 0.947979
Ran for 133050 frames Cell Events: 7476 mean_circ: 0.948007
Ran for 133550 frames Cell Events: 7497 mean_circ: 0.94804
Ran for 134050 frames Cell Events: 7524 mean_circ: 0.948078
Ran for 138550 frames Cell Events: 7805 mean_circ: 0.948073
Ran for 139050 frames Cell Events: 7827 mean_circ: 0.948073
Ran for 139550 frames Cell Events: 7849 mean_circ: 0.948043
Ran for 140050 frames Cell Events: 7894 mean_circ: 0.948023
Ran for 141050 frames Cell Events: 7960 mean_circ: 0.948026
Ran for 142050 frames Cell Events: 8008 mean_circ: 0.948014
Ran for 142550 frames Cell Events: 8033 mean_circ: 0.94802
Ran for 143550 frames Cell Events: 8086 mean_circ: 0.94803
End this measurment..
Stopping time: 1.9503e+009
****************************************
Measurement finished
Mean Circularitiy: 0.948036
Mean Area: 637.264
Mean FL-1 intensity: 4550.09
Mean FL-2 intensity: 5334.6
Mean FL-3 intensity: 0
frames processed: 143831
Total Time: 72824.4ms
Number of Runs (new bg img) 0
Frames collected: 145464
Skipped frames: 166662
Rescued Frames: 5223488
Samples DAQ: 36368016
Samples written: 72731990
Samples per Channel: 36365995
FPS: 1997.46
Samples per second: 499366
Expected samples/s: 500000
Events: 8198
Events per second: 112.572
jumped back (PMT): 3783
#Frames read, frequency
# 0 frames :216157
# 1 frames :61626
# 2 frames :3403
# 3 frames :1143
# 4 frames :539
# 5 frames :1867
# 6 frames :1538
# 7 frames :1242
# 8 frames :688
# 9 frames :467
# 10 frames :360
# 11 frames :299
# 12 frames :319
# 13 frames :231
# 14 frames :154
# 15 frames :129
# 16 frames :109
# 17 frames :112
# 18 frames :89
# 19 frames :72
# 20 frames :41
# 21 frames :51
# 22 frames :40
# 23 frames :30
# 24 frames :21
# 25 frames :15
# 26 frames :14
# 27 frames :17
# 28 frames :11
# 29 frames :17
# 30 frames :8
# 31 frames :6
# 32 frames :15
# 33 frames :3
# 34 frames :6
# 35 frames :6
# 36 frames :4
# 37 frames :3
# 38 frames :4
# 41 frames :3
# 42 frames :3
# 46 frames :3
# 47 frames :1
# 48 frames :1
# 49 frames :1
# 50 frames :2
# 51 frames :1
# 52 frames :1
# 55 frames :2
# 68 frames :1
# 89 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.24
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0