M1_20170608_1306_NK1_col_scalar.rtdc
| Date of measurement | 2017-06-08 |
|---|---|
| Index of measurement run | 1 |
| Sample name | 20170608_1306_NK1_col |
| Number of recorded events | 7035 |
| Start time of measurement | 13:06:41 |
| Unique measurement identifier | None-7365-03bf |
| Software | fRT-DC V1.06170206 | dclab 0.60.3 | dclab 0.59.1 | dclab 0.62.14 |
|---|---|
| Modules used | Cell_Flow_2, Fluor |
| Flow rate in channel | 0.04 µL/s |
| Imaged chip region | channel |
| Width of microfluidic channel | 20 µm |
| Image height | 96 px |
|---|---|
| Image width | 256 px |
| Image x coordinate on sensor | 504 px |
| Image y coordinate on sensor | 472 px |
| Imaging frame rate | 2000 Hz |
| Light source device type | LED (undefined) |
| Light source flash duration | 2 µs |
| Pixel size | 0.34 µm |
| FL1 description | FL1 |
|---|---|
| FL2 description | FL2 |
| Laser 1 output power | 10 % |
| Laser 1 wavelength | 488 nm |
| Laser 2 output power | 35 % |
| Laser 2 wavelength | 561 nm |
| Laser 3 output power | 0 % |
| Laser 3 wavelength | 640 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 2 |
| Number of active lasers | 2 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 5 |
| Samples per event | 300 |
| Trace bit depth | 16 |
| Trace sample rate | 5e+05 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 3 months ago |
| etag | 1c9748b829f8e81b562ce0bfdc0acf68 |
| format | RT-FDC |
| id | 3eb1c621-464e-4d58-aa01-1fcca2efaf41 |
| metadata modified | 3 months ago |
| mimetype | RT-DC |
| package id | df1c196f-b894-4f6b-9ac6-701137126e39 |
| position | 1 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/3eb/1c6/21-464e-4d58-aa01-1fcca2efaf41 |
| sha256 | b43c52a26905bb5b765db82c6d117e235dc376e9418b8e5dea795ba9af15cc96 |
| size | 275.6 KiB |
| dc:experiment:date | 2017-06-08 |
| dc:experiment:event count | 7,035 |
| dc:experiment:run identifier | None-7365-03bf |
| dc:experiment:run index | 1 |
| dc:experiment:sample | 20170608_1306_NK1_col |
| dc:experiment:time | 13:06:41 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | FL1 |
| dc:fluorescence:channel 2 name | FL2 |
| dc:fluorescence:channel count | 2 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 1 lambda | 488 |
| dc:fluorescence:laser 1 power | 10 |
| dc:fluorescence:laser 2 lambda | 561 |
| dc:fluorescence:laser 2 power | 35 |
| dc:fluorescence:laser 3 lambda | 640 |
| dc:fluorescence:laser 3 power | 0 |
| dc:fluorescence:laser count | 2 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 500,000 |
| dc:fluorescence:samples per event | 300 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 5 |
| dc:imaging:flash device | LED (undefined) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 2,000 |
| dc:imaging:pixel size | 0.34 |
| dc:imaging:roi position x | 504 |
| dc:imaging:roi position y | 472 |
| dc:imaging:roi size x | 256 |
| dc:imaging:roi size y | 96 |
| dc:online contour:bin area min | 50 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | 6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.04 |
| dc:setup:module composition | Cell_Flow_2, Fluor |
| dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 | dclab 0.59.1 | dclab 0.62.14 |
| sp:cells:cell type | KC167 |
| sp:cells:live | True |
| sp:cells:organism | Drosophila melanogaster |
| sp:chip:channel width | 20 |
| sp:experiment:buffer osmolality | 325 |
| sp:experiment:buffer ph | 6.8 |
| sp:experiment:concentration | 5 |
| sp:identifiers:labels | GFP tdTomato |
| sp:identifiers:tags | NLS-GFP |