Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.21.30
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c60da64bfdeb64709fc0f5ab5cb727ab",
"name": "M1_20170608_1446_dsRNA_T8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:21:51"
}
}
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.587
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.09663e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.938428
Ran for 1050 frames Cell Events: 37 mean_circ: 0.953354
Ran for 2550 frames Cell Events: 102 mean_circ: 0.950151
Ran for 3550 frames Cell Events: 130 mean_circ: 0.950585
Ran for 4050 frames Cell Events: 143 mean_circ: 0.950311
Ran for 4550 frames Cell Events: 167 mean_circ: 0.950078
Ran for 5550 frames Cell Events: 213 mean_circ: 0.950048
Ran for 6050 frames Cell Events: 236 mean_circ: 0.950072
Ran for 6550 frames Cell Events: 251 mean_circ: 0.949204
Ran for 7550 frames Cell Events: 277 mean_circ: 0.949664
Ran for 9050 frames Cell Events: 322 mean_circ: 0.949918
Ran for 9550 frames Cell Events: 342 mean_circ: 0.950273
Ran for 10050 frames Cell Events: 353 mean_circ: 0.95035
Ran for 11050 frames Cell Events: 384 mean_circ: 0.95068
Ran for 11550 frames Cell Events: 400 mean_circ: 0.951211
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Ran for 15050 frames Cell Events: 497 mean_circ: 0.952008
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Ran for 16550 frames Cell Events: 539 mean_circ: 0.952328
Ran for 17050 frames Cell Events: 547 mean_circ: 0.952377
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Ran for 20050 frames Cell Events: 643 mean_circ: 0.951438
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Ran for 23550 frames Cell Events: 757 mean_circ: 0.951692
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Ran for 25050 frames Cell Events: 806 mean_circ: 0.951459
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Ran for 37050 frames Cell Events: 1252 mean_circ: 0.952734
Ran for 37550 frames Cell Events: 1277 mean_circ: 0.952727
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Ran for 72550 frames Cell Events: 2390 mean_circ: 0.953022
Ran for 73050 frames Cell Events: 2404 mean_circ: 0.952958
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Ran for 78550 frames Cell Events: 2586 mean_circ: 0.952774
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Ran for 84550 frames Cell Events: 2771 mean_circ: 0.952813
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Ran for 86550 frames Cell Events: 2849 mean_circ: 0.952706
Ran for 87050 frames Cell Events: 2867 mean_circ: 0.95265
Ran for 87550 frames Cell Events: 2881 mean_circ: 0.952686
Ran for 88050 frames Cell Events: 2897 mean_circ: 0.952631
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Ran for 91050 frames Cell Events: 2999 mean_circ: 0.952477
Ran for 92050 frames Cell Events: 3041 mean_circ: 0.952457
Ran for 93050 frames Cell Events: 3083 mean_circ: 0.952424
Ran for 94050 frames Cell Events: 3109 mean_circ: 0.952358
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Ran for 95550 frames Cell Events: 3147 mean_circ: 0.952298
Ran for 98050 frames Cell Events: 3219 mean_circ: 0.952353
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Ran for 99550 frames Cell Events: 3264 mean_circ: 0.952446
Ran for 100050 frames Cell Events: 3283 mean_circ: 0.952446
Ran for 102050 frames Cell Events: 3348 mean_circ: 0.952582
Ran for 102550 frames Cell Events: 3366 mean_circ: 0.952523
Ran for 103050 frames Cell Events: 3382 mean_circ: 0.952537
Ran for 105550 frames Cell Events: 3471 mean_circ: 0.952561
Ran for 106050 frames Cell Events: 3485 mean_circ: 0.952605
Ran for 106550 frames Cell Events: 3496 mean_circ: 0.952634
Ran for 107050 frames Cell Events: 3511 mean_circ: 0.952629
Ran for 109550 frames Cell Events: 3609 mean_circ: 0.952575
Ran for 111050 frames Cell Events: 3662 mean_circ: 0.952597
Ran for 111550 frames Cell Events: 3674 mean_circ: 0.95261
Ran for 112550 frames Cell Events: 3711 mean_circ: 0.952643
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Ran for 121050 frames Cell Events: 3993 mean_circ: 0.952546
Ran for 121550 frames Cell Events: 4010 mean_circ: 0.952647
Ran for 122050 frames Cell Events: 4030 mean_circ: 0.952658
Ran for 122550 frames Cell Events: 4053 mean_circ: 0.95268
Ran for 123550 frames Cell Events: 4091 mean_circ: 0.952636
Ran for 124550 frames Cell Events: 4131 mean_circ: 0.952662
Ran for 126050 frames Cell Events: 4180 mean_circ: 0.952564
Ran for 126550 frames Cell Events: 4195 mean_circ: 0.952504
Ran for 127050 frames Cell Events: 4216 mean_circ: 0.952507
Ran for 127550 frames Cell Events: 4231 mean_circ: 0.952477
Ran for 128550 frames Cell Events: 4263 mean_circ: 0.952472
Ran for 130050 frames Cell Events: 4314 mean_circ: 0.952509
Ran for 130550 frames Cell Events: 4324 mean_circ: 0.952464
Ran for 131050 frames Cell Events: 4335 mean_circ: 0.952479
Ran for 132050 frames Cell Events: 4360 mean_circ: 0.952443
Ran for 132550 frames Cell Events: 4376 mean_circ: 0.95245
Ran for 136050 frames Cell Events: 4489 mean_circ: 0.952341
Ran for 137050 frames Cell Events: 4528 mean_circ: 0.952267
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Ran for 139550 frames Cell Events: 4621 mean_circ: 0.952278
Ran for 141550 frames Cell Events: 4681 mean_circ: 0.952237
Ran for 142050 frames Cell Events: 4693 mean_circ: 0.952257
Ran for 142550 frames Cell Events: 4712 mean_circ: 0.952267
Ran for 144050 frames Cell Events: 4765 mean_circ: 0.952328
Ran for 146550 frames Cell Events: 4843 mean_circ: 0.952324
Ran for 150050 frames Cell Events: 4969 mean_circ: 0.952247
Ran for 150550 frames Cell Events: 4986 mean_circ: 0.952161
Ran for 151550 frames Cell Events: 5013 mean_circ: 0.952123
Ran for 152050 frames Cell Events: 5040 mean_circ: 0.952142
Ran for 152550 frames Cell Events: 5063 mean_circ: 0.952102
Ran for 153050 frames Cell Events: 5073 mean_circ: 0.952082
Ran for 157050 frames Cell Events: 5196 mean_circ: 0.952138
Ran for 157550 frames Cell Events: 5210 mean_circ: 0.95215
Ran for 158050 frames Cell Events: 5220 mean_circ: 0.952179
Ran for 159050 frames Cell Events: 5257 mean_circ: 0.952185
Ran for 160050 frames Cell Events: 5302 mean_circ: 0.952202
Ran for 161050 frames Cell Events: 5333 mean_circ: 0.952212
Ran for 161550 frames Cell Events: 5342 mean_circ: 0.952231
Ran for 162050 frames Cell Events: 5365 mean_circ: 0.952189
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Ran for 167050 frames Cell Events: 5553 mean_circ: 0.952151
Ran for 168050 frames Cell Events: 5590 mean_circ: 0.952226
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Ran for 171550 frames Cell Events: 5716 mean_circ: 0.952242
Ran for 172050 frames Cell Events: 5731 mean_circ: 0.952242
Ran for 174050 frames Cell Events: 5798 mean_circ: 0.952225
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Ran for 236550 frames Cell Events: 7993 mean_circ: 0.951989
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Ran for 238050 frames Cell Events: 8051 mean_circ: 0.951998
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Ran for 239550 frames Cell Events: 8099 mean_circ: 0.952007
Ran for 240050 frames Cell Events: 8117 mean_circ: 0.951976
Ran for 241050 frames Cell Events: 8152 mean_circ: 0.951956
End this measurment..
Stopping time: 2.09675e+009
****************************************
Measurement finished
Mean Circularitiy: 0.951976
Mean Area: 633.941
Mean FL-1 intensity: 4301.6
Mean FL-2 intensity: 4788.64
Mean FL-3 intensity: 0
frames processed: 239878
Total Time: 120832ms
Number of Runs (new bg img) 0
Frames collected: 241520
Skipped frames: 27742
Rescued Frames: 1790274
Samples DAQ: 60382409
Samples written: 120759772
Samples per Channel: 60379886
FPS: 1998.81
Samples per second: 499701
Expected samples/s: 500000
Events: 8170
Events per second: 67.6145
jumped back (PMT): 4020
#Frames read, frequency
# 0 frames :365907
# 1 frames :98991
# 2 frames :3906
# 3 frames :1980
# 4 frames :1087
# 5 frames :789
# 6 frames :760
# 7 frames :1181
# 8 frames :1170
# 9 frames :1011
# 10 frames :923
# 11 frames :750
# 12 frames :557
# 13 frames :437
# 14 frames :318
# 15 frames :287
# 16 frames :263
# 17 frames :294
# 18 frames :262
# 19 frames :260
# 20 frames :174
# 21 frames :141
# 22 frames :128
# 23 frames :88
# 24 frames :56
# 25 frames :62
# 26 frames :72
# 27 frames :60
# 28 frames :38
# 29 frames :38
# 30 frames :32
# 31 frames :31
# 32 frames :27
# 33 frames :30
# 34 frames :14
# 35 frames :17
# 36 frames :15
# 37 frames :15
# 38 frames :12
# 39 frames :8
# 40 frames :8
# 41 frames :12
# 42 frames :10
# 43 frames :8
# 44 frames :6
# 45 frames :7
# 46 frames :5
# 47 frames :4
# 48 frames :7
# 49 frames :9
# 50 frames :4
# 51 frames :3
# 52 frames :3
# 53 frames :2
# 54 frames :2
# 55 frames :3
# 56 frames :1
# 57 frames :1
# 58 frames :1
# 59 frames :1
# 61 frames :1
# 62 frames :2
# 64 frames :2
# 65 frames :1
# 66 frames :1
# 67 frames :1
# 68 frames :1
# 72 frames :1
# 73 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.587
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0