Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.23.37
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2ca5d99b11f3079b1c39797115d0bd49",
"name": "M1_20170608_1511_dsRNA_U2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:24:03"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9488 (max 9490) in '9488'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5142
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.09812e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 37 mean_circ: 0.912018
Ran for 1550 frames Cell Events: 63 mean_circ: 0.919129
Ran for 2050 frames Cell Events: 85 mean_circ: 0.93152
Ran for 4050 frames Cell Events: 171 mean_circ: 0.93827
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Ran for 5050 frames Cell Events: 201 mean_circ: 0.942665
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Ran for 86550 frames Cell Events: 2903 mean_circ: 0.946418
Ran for 87050 frames Cell Events: 2914 mean_circ: 0.946427
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Ran for 93550 frames Cell Events: 3159 mean_circ: 0.946455
Ran for 95050 frames Cell Events: 3218 mean_circ: 0.946435
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Ran for 96550 frames Cell Events: 3259 mean_circ: 0.946538
Ran for 97050 frames Cell Events: 3271 mean_circ: 0.946497
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Ran for 100050 frames Cell Events: 3369 mean_circ: 0.946669
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Ran for 104050 frames Cell Events: 3540 mean_circ: 0.946861
Ran for 104550 frames Cell Events: 3559 mean_circ: 0.946761
Ran for 105050 frames Cell Events: 3571 mean_circ: 0.946764
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Ran for 106050 frames Cell Events: 3614 mean_circ: 0.946594
Ran for 108050 frames Cell Events: 3703 mean_circ: 0.946343
Ran for 108550 frames Cell Events: 3716 mean_circ: 0.946381
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Ran for 122550 frames Cell Events: 4191 mean_circ: 0.946593
Ran for 123050 frames Cell Events: 4206 mean_circ: 0.946627
Ran for 123550 frames Cell Events: 4218 mean_circ: 0.94657
Ran for 124050 frames Cell Events: 4231 mean_circ: 0.94661
Ran for 124550 frames Cell Events: 4246 mean_circ: 0.946603
Ran for 125050 frames Cell Events: 4271 mean_circ: 0.946669
Ran for 126550 frames Cell Events: 4327 mean_circ: 0.946776
Ran for 128050 frames Cell Events: 4390 mean_circ: 0.94685
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Ran for 129050 frames Cell Events: 4415 mean_circ: 0.946931
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Ran for 131050 frames Cell Events: 4499 mean_circ: 0.946908
Ran for 131550 frames Cell Events: 4511 mean_circ: 0.946871
Ran for 132050 frames Cell Events: 4522 mean_circ: 0.946849
Ran for 132550 frames Cell Events: 4554 mean_circ: 0.946878
Ran for 133550 frames Cell Events: 4593 mean_circ: 0.946779
Ran for 134050 frames Cell Events: 4606 mean_circ: 0.946759
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Ran for 139550 frames Cell Events: 4792 mean_circ: 0.946658
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Ran for 141550 frames Cell Events: 4863 mean_circ: 0.946781
Ran for 142050 frames Cell Events: 4884 mean_circ: 0.946823
Ran for 143550 frames Cell Events: 4935 mean_circ: 0.946921
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Ran for 145050 frames Cell Events: 4996 mean_circ: 0.946918
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Ran for 200050 frames Cell Events: 7015 mean_circ: 0.947431
Ran for 202050 frames Cell Events: 7079 mean_circ: 0.947468
Ran for 203050 frames Cell Events: 7105 mean_circ: 0.947486
Ran for 203550 frames Cell Events: 7124 mean_circ: 0.947484
Ran for 204050 frames Cell Events: 7141 mean_circ: 0.947455
Ran for 204550 frames Cell Events: 7160 mean_circ: 0.947427
Ran for 205050 frames Cell Events: 7177 mean_circ: 0.947377
Ran for 206050 frames Cell Events: 7213 mean_circ: 0.947311
Ran for 207550 frames Cell Events: 7294 mean_circ: 0.947352
Ran for 208050 frames Cell Events: 7315 mean_circ: 0.947371
Ran for 208550 frames Cell Events: 7338 mean_circ: 0.947383
Ran for 209050 frames Cell Events: 7352 mean_circ: 0.947398
Ran for 209550 frames Cell Events: 7362 mean_circ: 0.947365
Ran for 210550 frames Cell Events: 7400 mean_circ: 0.947354
Ran for 211050 frames Cell Events: 7419 mean_circ: 0.947321
Ran for 212050 frames Cell Events: 7457 mean_circ: 0.94733
Ran for 213050 frames Cell Events: 7501 mean_circ: 0.947334
Ran for 214050 frames Cell Events: 7527 mean_circ: 0.947326
Ran for 214550 frames Cell Events: 7541 mean_circ: 0.947323
Ran for 215050 frames Cell Events: 7556 mean_circ: 0.947335
Ran for 215550 frames Cell Events: 7574 mean_circ: 0.947327
Ran for 217050 frames Cell Events: 7625 mean_circ: 0.947319
Ran for 218550 frames Cell Events: 7684 mean_circ: 0.947368
Ran for 219550 frames Cell Events: 7733 mean_circ: 0.947367
Ran for 220050 frames Cell Events: 7745 mean_circ: 0.947372
Ran for 220550 frames Cell Events: 7761 mean_circ: 0.947377
Ran for 221050 frames Cell Events: 7786 mean_circ: 0.947381
Ran for 221550 frames Cell Events: 7807 mean_circ: 0.947377
Ran for 223550 frames Cell Events: 7890 mean_circ: 0.94733
Ran for 224050 frames Cell Events: 7921 mean_circ: 0.947335
Ran for 224550 frames Cell Events: 7927 mean_circ: 0.947332
Ran for 225550 frames Cell Events: 7975 mean_circ: 0.947297
Ran for 226050 frames Cell Events: 8004 mean_circ: 0.947296
Ran for 226550 frames Cell Events: 8022 mean_circ: 0.947281
Ran for 228050 frames Cell Events: 8079 mean_circ: 0.947212
Ran for 228550 frames Cell Events: 8092 mean_circ: 0.947198
Ran for 229550 frames Cell Events: 8120 mean_circ: 0.94721
Ran for 230050 frames Cell Events: 8141 mean_circ: 0.947223
Ran for 230550 frames Cell Events: 8161 mean_circ: 0.947241
Ran for 231050 frames Cell Events: 8181 mean_circ: 0.947225
Ran for 231550 frames Cell Events: 8194 mean_circ: 0.947249
Ran for 232050 frames Cell Events: 8211 mean_circ: 0.947265
Ran for 233050 frames Cell Events: 8248 mean_circ: 0.947278
Ran for 233550 frames Cell Events: 8263 mean_circ: 0.947307
Ran for 234050 frames Cell Events: 8279 mean_circ: 0.947292
Ran for 234550 frames Cell Events: 8295 mean_circ: 0.947288
Ran for 235050 frames Cell Events: 8319 mean_circ: 0.947286
Ran for 235550 frames Cell Events: 8344 mean_circ: 0.947325
Ran for 236050 frames Cell Events: 8366 mean_circ: 0.947322
Ran for 236550 frames Cell Events: 8384 mean_circ: 0.947332
Ran for 237050 frames Cell Events: 8402 mean_circ: 0.947344
Ran for 237550 frames Cell Events: 8428 mean_circ: 0.947379
Ran for 238050 frames Cell Events: 8440 mean_circ: 0.947358
Ran for 238550 frames Cell Events: 8454 mean_circ: 0.947361
Ran for 240550 frames Cell Events: 8557 mean_circ: 0.947329
Ran for 242050 frames Cell Events: 8602 mean_circ: 0.947369
Ran for 242550 frames Cell Events: 8618 mean_circ: 0.947365
Ran for 243050 frames Cell Events: 8635 mean_circ: 0.947348
Ran for 243550 frames Cell Events: 8648 mean_circ: 0.947365
Ran for 244050 frames Cell Events: 8657 mean_circ: 0.947365
Ran for 244550 frames Cell Events: 8677 mean_circ: 0.947379
Ran for 245550 frames Cell Events: 8717 mean_circ: 0.947408
Ran for 247550 frames Cell Events: 8812 mean_circ: 0.947343
Ran for 248050 frames Cell Events: 8833 mean_circ: 0.947356
Ran for 248550 frames Cell Events: 8854 mean_circ: 0.947367
Ran for 249050 frames Cell Events: 8870 mean_circ: 0.947395
Ran for 249550 frames Cell Events: 8891 mean_circ: 0.947406
Ran for 250050 frames Cell Events: 8910 mean_circ: 0.947394
Ran for 250550 frames Cell Events: 8927 mean_circ: 0.947389
Ran for 251550 frames Cell Events: 8955 mean_circ: 0.947384
Ran for 252050 frames Cell Events: 8973 mean_circ: 0.947389
Ran for 253050 frames Cell Events: 9010 mean_circ: 0.947387
Ran for 254550 frames Cell Events: 9055 mean_circ: 0.947399
Ran for 255050 frames Cell Events: 9083 mean_circ: 0.947381
Ran for 256050 frames Cell Events: 9113 mean_circ: 0.947385
Ran for 256550 frames Cell Events: 9135 mean_circ: 0.947391
Ran for 257050 frames Cell Events: 9148 mean_circ: 0.947388
Ran for 257550 frames Cell Events: 9161 mean_circ: 0.947405
Ran for 259550 frames Cell Events: 9250 mean_circ: 0.947413
Ran for 260050 frames Cell Events: 9271 mean_circ: 0.947443
Ran for 260550 frames Cell Events: 9286 mean_circ: 0.947437
Ran for 261050 frames Cell Events: 9305 mean_circ: 0.947429
Ran for 262050 frames Cell Events: 9338 mean_circ: 0.947453
Ran for 264050 frames Cell Events: 9421 mean_circ: 0.947454
Ran for 264550 frames Cell Events: 9441 mean_circ: 0.947465
Ran for 265550 frames Cell Events: 9482 mean_circ: 0.947426
End this measurment..
Stopping time: 2.09825e+009
****************************************
Measurement finished
Mean Circularitiy: 0.947433
Mean Area: 636.762
Mean FL-1 intensity: 4135.92
Mean FL-2 intensity: 4655.78
Mean FL-3 intensity: 0
frames processed: 265563
Total Time: 132992ms
Number of Runs (new bg img) 0
Frames collected: 265867
Skipped frames: 28713
Rescued Frames: 2194121
Samples DAQ: 66466681
Samples written: 132933282
Samples per Channel: 66466641
FPS: 1999.12
Samples per second: 499779
Expected samples/s: 500000
Events: 9490
Events per second: 71.3577
jumped back (PMT): 4912
#Frames read, frequency
# 0 frames :382726
# 1 frames :129437
# 2 frames :5319
# 3 frames :1956
# 4 frames :1088
# 5 frames :670
# 6 frames :1321
# 7 frames :2490
# 8 frames :1149
# 9 frames :812
# 10 frames :1053
# 11 frames :750
# 12 frames :532
# 13 frames :288
# 14 frames :213
# 15 frames :201
# 16 frames :213
# 17 frames :195
# 18 frames :241
# 19 frames :288
# 20 frames :174
# 21 frames :115
# 22 frames :112
# 23 frames :60
# 24 frames :52
# 25 frames :38
# 26 frames :33
# 27 frames :39
# 28 frames :28
# 29 frames :16
# 30 frames :25
# 31 frames :9
# 32 frames :12
# 33 frames :10
# 34 frames :4
# 35 frames :8
# 36 frames :5
# 37 frames :1
# 38 frames :5
# 39 frames :3
# 40 frames :1
# 41 frames :3
# 42 frames :3
# 43 frames :4
# 45 frames :1
# 46 frames :2
# 47 frames :2
# 48 frames :1
# 49 frames :1
# 53 frames :1
# 54 frames :1
# 56 frames :1
# 57 frames :1
# 59 frames :1
# 81 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5142
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0