Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.32.56
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8610b50dc0ab35f4961b37180cfa9b6c",
"name": "M1_20170608_1820_dsRNA_V3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:33:18"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8235 (max 8236) in '8235'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8559
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.10947e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.934328
Ran for 550 frames Cell Events: 10 mean_circ: 0.942633
Ran for 1550 frames Cell Events: 42 mean_circ: 0.949675
Ran for 2550 frames Cell Events: 80 mean_circ: 0.93816
Ran for 3050 frames Cell Events: 91 mean_circ: 0.937624
Ran for 4550 frames Cell Events: 132 mean_circ: 0.940938
Ran for 5550 frames Cell Events: 178 mean_circ: 0.941733
Ran for 6050 frames Cell Events: 191 mean_circ: 0.940934
Ran for 6550 frames Cell Events: 212 mean_circ: 0.941221
Ran for 7050 frames Cell Events: 232 mean_circ: 0.94255
Ran for 7550 frames Cell Events: 248 mean_circ: 0.942218
Ran for 8050 frames Cell Events: 256 mean_circ: 0.942138
Ran for 8550 frames Cell Events: 267 mean_circ: 0.942763
Ran for 10050 frames Cell Events: 314 mean_circ: 0.941492
Ran for 11050 frames Cell Events: 346 mean_circ: 0.941393
Ran for 11550 frames Cell Events: 365 mean_circ: 0.941767
Ran for 13050 frames Cell Events: 410 mean_circ: 0.939983
Ran for 14050 frames Cell Events: 436 mean_circ: 0.940394
Ran for 14550 frames Cell Events: 456 mean_circ: 0.940867
Ran for 15050 frames Cell Events: 470 mean_circ: 0.940951
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Ran for 16050 frames Cell Events: 497 mean_circ: 0.940328
Ran for 16550 frames Cell Events: 512 mean_circ: 0.940394
Ran for 17550 frames Cell Events: 544 mean_circ: 0.940661
Ran for 18050 frames Cell Events: 556 mean_circ: 0.940957
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Ran for 62550 frames Cell Events: 1945 mean_circ: 0.947311
Ran for 64050 frames Cell Events: 1981 mean_circ: 0.947235
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Ran for 70550 frames Cell Events: 2177 mean_circ: 0.947203
Ran for 71550 frames Cell Events: 2209 mean_circ: 0.947163
Ran for 72550 frames Cell Events: 2243 mean_circ: 0.947198
Ran for 73050 frames Cell Events: 2259 mean_circ: 0.947235
Ran for 73550 frames Cell Events: 2274 mean_circ: 0.947052
Ran for 75050 frames Cell Events: 2325 mean_circ: 0.946989
Ran for 75550 frames Cell Events: 2340 mean_circ: 0.946931
Ran for 76050 frames Cell Events: 2360 mean_circ: 0.946987
Ran for 76550 frames Cell Events: 2378 mean_circ: 0.947036
Ran for 77050 frames Cell Events: 2400 mean_circ: 0.947046
Ran for 77550 frames Cell Events: 2414 mean_circ: 0.947065
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Ran for 81550 frames Cell Events: 2554 mean_circ: 0.947143
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Ran for 83050 frames Cell Events: 2590 mean_circ: 0.947072
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Ran for 84550 frames Cell Events: 2635 mean_circ: 0.94718
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Ran for 87050 frames Cell Events: 2732 mean_circ: 0.947219
Ran for 87550 frames Cell Events: 2745 mean_circ: 0.947214
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Ran for 88550 frames Cell Events: 2762 mean_circ: 0.947242
Ran for 89050 frames Cell Events: 2770 mean_circ: 0.947198
Ran for 90550 frames Cell Events: 2799 mean_circ: 0.9471
Ran for 91050 frames Cell Events: 2814 mean_circ: 0.947058
Ran for 91550 frames Cell Events: 2832 mean_circ: 0.947129
Ran for 92550 frames Cell Events: 2865 mean_circ: 0.946991
Ran for 93050 frames Cell Events: 2881 mean_circ: 0.946848
Ran for 93550 frames Cell Events: 2892 mean_circ: 0.946885
Ran for 94550 frames Cell Events: 2920 mean_circ: 0.946948
Ran for 95050 frames Cell Events: 2929 mean_circ: 0.946923
Ran for 96050 frames Cell Events: 2955 mean_circ: 0.946932
Ran for 96550 frames Cell Events: 2964 mean_circ: 0.946833
Ran for 97050 frames Cell Events: 2978 mean_circ: 0.946879
Ran for 99550 frames Cell Events: 3044 mean_circ: 0.946825
Ran for 101050 frames Cell Events: 3093 mean_circ: 0.946782
Ran for 102050 frames Cell Events: 3124 mean_circ: 0.946772
Ran for 102550 frames Cell Events: 3141 mean_circ: 0.946786
Ran for 103050 frames Cell Events: 3172 mean_circ: 0.946791
Ran for 103550 frames Cell Events: 3190 mean_circ: 0.946719
Ran for 104550 frames Cell Events: 3224 mean_circ: 0.946804
Ran for 105550 frames Cell Events: 3257 mean_circ: 0.946759
Ran for 106050 frames Cell Events: 3269 mean_circ: 0.94661
Ran for 107050 frames Cell Events: 3303 mean_circ: 0.946655
Ran for 108050 frames Cell Events: 3329 mean_circ: 0.946672
Ran for 110050 frames Cell Events: 3403 mean_circ: 0.946784
Ran for 110550 frames Cell Events: 3413 mean_circ: 0.946783
Ran for 111050 frames Cell Events: 3429 mean_circ: 0.946694
Ran for 111550 frames Cell Events: 3441 mean_circ: 0.94675
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Ran for 116050 frames Cell Events: 3608 mean_circ: 0.946888
Ran for 117050 frames Cell Events: 3648 mean_circ: 0.946818
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Ran for 121550 frames Cell Events: 3820 mean_circ: 0.946823
Ran for 123050 frames Cell Events: 3867 mean_circ: 0.946845
Ran for 123550 frames Cell Events: 3893 mean_circ: 0.946843
Ran for 124050 frames Cell Events: 3898 mean_circ: 0.946734
Ran for 125050 frames Cell Events: 3944 mean_circ: 0.946721
Ran for 126050 frames Cell Events: 3994 mean_circ: 0.946722
Ran for 126550 frames Cell Events: 4023 mean_circ: 0.946716
Ran for 127550 frames Cell Events: 4065 mean_circ: 0.946697
Ran for 128050 frames Cell Events: 4083 mean_circ: 0.946686
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Ran for 130550 frames Cell Events: 4169 mean_circ: 0.94667
Ran for 131050 frames Cell Events: 4188 mean_circ: 0.946665
Ran for 132050 frames Cell Events: 4228 mean_circ: 0.946713
Ran for 132550 frames Cell Events: 4243 mean_circ: 0.946694
Ran for 133550 frames Cell Events: 4258 mean_circ: 0.946694
Ran for 134050 frames Cell Events: 4266 mean_circ: 0.946694
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Ran for 138050 frames Cell Events: 4393 mean_circ: 0.946652
Ran for 138550 frames Cell Events: 4409 mean_circ: 0.946628
Ran for 139050 frames Cell Events: 4424 mean_circ: 0.946629
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Ran for 140550 frames Cell Events: 4461 mean_circ: 0.94657
Ran for 141550 frames Cell Events: 4495 mean_circ: 0.946598
Ran for 142050 frames Cell Events: 4512 mean_circ: 0.946618
Ran for 142550 frames Cell Events: 4529 mean_circ: 0.946634
Ran for 143550 frames Cell Events: 4552 mean_circ: 0.946632
Ran for 144550 frames Cell Events: 4595 mean_circ: 0.946668
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Ran for 145550 frames Cell Events: 4632 mean_circ: 0.946704
Ran for 146050 frames Cell Events: 4650 mean_circ: 0.946708
Ran for 147050 frames Cell Events: 4690 mean_circ: 0.946774
Ran for 147550 frames Cell Events: 4701 mean_circ: 0.946773
Ran for 148050 frames Cell Events: 4715 mean_circ: 0.946748
Ran for 149050 frames Cell Events: 4752 mean_circ: 0.946702
Ran for 150050 frames Cell Events: 4778 mean_circ: 0.946679
Ran for 150550 frames Cell Events: 4799 mean_circ: 0.946701
Ran for 151550 frames Cell Events: 4843 mean_circ: 0.946762
Ran for 152550 frames Cell Events: 4880 mean_circ: 0.946758
Ran for 154050 frames Cell Events: 4910 mean_circ: 0.946735
Ran for 154550 frames Cell Events: 4923 mean_circ: 0.946775
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Ran for 156050 frames Cell Events: 4981 mean_circ: 0.946813
Ran for 156550 frames Cell Events: 4995 mean_circ: 0.946805
Ran for 157050 frames Cell Events: 5013 mean_circ: 0.946782
Ran for 158050 frames Cell Events: 5049 mean_circ: 0.946819
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Ran for 201050 frames Cell Events: 6497 mean_circ: 0.946729
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Ran for 212050 frames Cell Events: 6871 mean_circ: 0.946641
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Ran for 213050 frames Cell Events: 6908 mean_circ: 0.946659
Ran for 214550 frames Cell Events: 6953 mean_circ: 0.946602
Ran for 215050 frames Cell Events: 6963 mean_circ: 0.946571
Ran for 216050 frames Cell Events: 6990 mean_circ: 0.946517
Ran for 218050 frames Cell Events: 7050 mean_circ: 0.946488
Ran for 218550 frames Cell Events: 7071 mean_circ: 0.946479
Ran for 219050 frames Cell Events: 7079 mean_circ: 0.946475
Ran for 220050 frames Cell Events: 7103 mean_circ: 0.946454
Ran for 221050 frames Cell Events: 7144 mean_circ: 0.946417
Ran for 221550 frames Cell Events: 7159 mean_circ: 0.946451
Ran for 222550 frames Cell Events: 7188 mean_circ: 0.946487
Ran for 223050 frames Cell Events: 7208 mean_circ: 0.946499
Ran for 224550 frames Cell Events: 7269 mean_circ: 0.94639
Ran for 225050 frames Cell Events: 7285 mean_circ: 0.946438
Ran for 225550 frames Cell Events: 7290 mean_circ: 0.94645
Ran for 226050 frames Cell Events: 7306 mean_circ: 0.94645
Ran for 226550 frames Cell Events: 7329 mean_circ: 0.946391
Ran for 227050 frames Cell Events: 7342 mean_circ: 0.946438
Ran for 228550 frames Cell Events: 7384 mean_circ: 0.946498
Ran for 229050 frames Cell Events: 7396 mean_circ: 0.946509
Ran for 230050 frames Cell Events: 7430 mean_circ: 0.946506
Ran for 230550 frames Cell Events: 7443 mean_circ: 0.9465
Ran for 231050 frames Cell Events: 7459 mean_circ: 0.946515
Ran for 231550 frames Cell Events: 7471 mean_circ: 0.946517
Ran for 232050 frames Cell Events: 7485 mean_circ: 0.94655
Ran for 233050 frames Cell Events: 7505 mean_circ: 0.946512
Ran for 233550 frames Cell Events: 7528 mean_circ: 0.946478
Ran for 234050 frames Cell Events: 7542 mean_circ: 0.946459
Ran for 234550 frames Cell Events: 7553 mean_circ: 0.946453
Ran for 235050 frames Cell Events: 7574 mean_circ: 0.946417
Ran for 235550 frames Cell Events: 7589 mean_circ: 0.946477
Ran for 236050 frames Cell Events: 7596 mean_circ: 0.946491
Ran for 236550 frames Cell Events: 7602 mean_circ: 0.946495
Ran for 237550 frames Cell Events: 7634 mean_circ: 0.946534
Ran for 238050 frames Cell Events: 7651 mean_circ: 0.946555
Ran for 238550 frames Cell Events: 7674 mean_circ: 0.946523
Ran for 240050 frames Cell Events: 7719 mean_circ: 0.946477
Ran for 240550 frames Cell Events: 7736 mean_circ: 0.946411
Ran for 242550 frames Cell Events: 7795 mean_circ: 0.946411
Ran for 243050 frames Cell Events: 7806 mean_circ: 0.946431
Ran for 244050 frames Cell Events: 7834 mean_circ: 0.946443
Ran for 244550 frames Cell Events: 7863 mean_circ: 0.94644
Ran for 245050 frames Cell Events: 7871 mean_circ: 0.946436
Ran for 246050 frames Cell Events: 7896 mean_circ: 0.946441
Ran for 246550 frames Cell Events: 7928 mean_circ: 0.94646
Ran for 247050 frames Cell Events: 7951 mean_circ: 0.946464
Ran for 247550 frames Cell Events: 7960 mean_circ: 0.946485
Ran for 248550 frames Cell Events: 7991 mean_circ: 0.946501
Ran for 249050 frames Cell Events: 8020 mean_circ: 0.946501
Ran for 249550 frames Cell Events: 8031 mean_circ: 0.946466
Ran for 250050 frames Cell Events: 8048 mean_circ: 0.946465
Ran for 250550 frames Cell Events: 8059 mean_circ: 0.94648
Ran for 251050 frames Cell Events: 8072 mean_circ: 0.946455
Ran for 252550 frames Cell Events: 8133 mean_circ: 0.946466
Ran for 254550 frames Cell Events: 8198 mean_circ: 0.946413
Ran for 255050 frames Cell Events: 8208 mean_circ: 0.946414
Ran for 255550 frames Cell Events: 8234 mean_circ: 0.946428
End this measurment..
Stopping time: 2.10959e+009
****************************************
Measurement finished
Mean Circularitiy: 0.946439
Mean Area: 664.538
Mean FL-1 intensity: 3811.05
Mean FL-2 intensity: 4470.13
Mean FL-3 intensity: 0
frames processed: 254600
Total Time: 127880ms
Number of Runs (new bg img) 0
Frames collected: 255644
Skipped frames: 10174
Rescued Frames: 949540
Samples DAQ: 63910974
Samples written: 127821736
Samples per Channel: 63910868
FPS: 1999.09
Samples per second: 499771
Expected samples/s: 500000
Events: 8236
Events per second: 64.404
jumped back (PMT): 4452
#Frames read, frequency
# 0 frames :374960
# 1 frames :117583
# 2 frames :4569
# 3 frames :2144
# 4 frames :1279
# 5 frames :718
# 6 frames :822
# 7 frames :1520
# 8 frames :1326
# 9 frames :934
# 10 frames :820
# 11 frames :796
# 12 frames :690
# 13 frames :440
# 14 frames :344
# 15 frames :271
# 16 frames :234
# 17 frames :264
# 18 frames :221
# 19 frames :188
# 20 frames :144
# 21 frames :125
# 22 frames :110
# 23 frames :69
# 24 frames :67
# 25 frames :37
# 26 frames :46
# 27 frames :34
# 28 frames :38
# 29 frames :29
# 30 frames :20
# 31 frames :21
# 32 frames :20
# 33 frames :14
# 34 frames :12
# 35 frames :11
# 36 frames :6
# 37 frames :13
# 38 frames :8
# 39 frames :12
# 40 frames :5
# 41 frames :6
# 42 frames :3
# 43 frames :4
# 44 frames :5
# 45 frames :5
# 46 frames :4
# 47 frames :3
# 49 frames :7
# 51 frames :4
# 52 frames :3
# 53 frames :1
# 54 frames :1
# 56 frames :1
# 57 frames :3
# 61 frames :1
# 63 frames :1
# 69 frames :1
# 73 frames :1
# 84 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8559
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0