Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.35.31
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "499b10874f7bc1c21b06b0a1c96e28a9",
"name": "M1_20170608_1849_dsRNA_R1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:35:52"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8052 (max 8054) in '8052'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9608
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.1112e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 12 mean_circ: 0.716794
Ran for 1050 frames Cell Events: 28 mean_circ: 0.917863
Ran for 1550 frames Cell Events: 45 mean_circ: 0.924418
Ran for 2050 frames Cell Events: 62 mean_circ: 0.928068
Ran for 2550 frames Cell Events: 70 mean_circ: 0.928174
Ran for 3050 frames Cell Events: 85 mean_circ: 0.928278
Ran for 4550 frames Cell Events: 145 mean_circ: 0.937749
Ran for 5550 frames Cell Events: 179 mean_circ: 0.940038
Ran for 6550 frames Cell Events: 212 mean_circ: 0.941076
Ran for 7050 frames Cell Events: 231 mean_circ: 0.941326
Ran for 7550 frames Cell Events: 247 mean_circ: 0.940638
Ran for 8050 frames Cell Events: 268 mean_circ: 0.939768
Ran for 9550 frames Cell Events: 317 mean_circ: 0.940945
Ran for 10050 frames Cell Events: 331 mean_circ: 0.941381
Ran for 10550 frames Cell Events: 344 mean_circ: 0.941183
Ran for 11550 frames Cell Events: 384 mean_circ: 0.942447
Ran for 12050 frames Cell Events: 405 mean_circ: 0.942318
Ran for 12550 frames Cell Events: 424 mean_circ: 0.941961
Ran for 13050 frames Cell Events: 439 mean_circ: 0.941878
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Ran for 14550 frames Cell Events: 499 mean_circ: 0.941807
Ran for 15050 frames Cell Events: 510 mean_circ: 0.942047
Ran for 16550 frames Cell Events: 563 mean_circ: 0.942594
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Ran for 33550 frames Cell Events: 1167 mean_circ: 0.940061
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Ran for 36550 frames Cell Events: 1268 mean_circ: 0.940087
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Ran for 38550 frames Cell Events: 1326 mean_circ: 0.939821
Ran for 39050 frames Cell Events: 1340 mean_circ: 0.939889
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Ran for 56050 frames Cell Events: 1971 mean_circ: 0.939621
Ran for 56550 frames Cell Events: 1995 mean_circ: 0.939615
Ran for 57050 frames Cell Events: 2017 mean_circ: 0.93961
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Ran for 62550 frames Cell Events: 2230 mean_circ: 0.939303
Ran for 63550 frames Cell Events: 2262 mean_circ: 0.939339
Ran for 64550 frames Cell Events: 2303 mean_circ: 0.939442
Ran for 65050 frames Cell Events: 2322 mean_circ: 0.939165
Ran for 65550 frames Cell Events: 2344 mean_circ: 0.939207
Ran for 66050 frames Cell Events: 2361 mean_circ: 0.939227
Ran for 66550 frames Cell Events: 2388 mean_circ: 0.939236
Ran for 67050 frames Cell Events: 2405 mean_circ: 0.939221
Ran for 68050 frames Cell Events: 2440 mean_circ: 0.939234
Ran for 68550 frames Cell Events: 2469 mean_circ: 0.939287
Ran for 70050 frames Cell Events: 2522 mean_circ: 0.939428
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Ran for 71050 frames Cell Events: 2555 mean_circ: 0.939318
Ran for 71550 frames Cell Events: 2588 mean_circ: 0.939361
Ran for 72050 frames Cell Events: 2612 mean_circ: 0.939415
Ran for 73050 frames Cell Events: 2648 mean_circ: 0.939405
Ran for 73550 frames Cell Events: 2665 mean_circ: 0.939478
Ran for 74550 frames Cell Events: 2695 mean_circ: 0.939497
Ran for 75050 frames Cell Events: 2714 mean_circ: 0.939518
Ran for 76050 frames Cell Events: 2745 mean_circ: 0.93942
Ran for 76550 frames Cell Events: 2762 mean_circ: 0.939433
Ran for 78050 frames Cell Events: 2827 mean_circ: 0.939366
Ran for 78550 frames Cell Events: 2842 mean_circ: 0.939386
Ran for 79550 frames Cell Events: 2884 mean_circ: 0.939342
Ran for 80050 frames Cell Events: 2898 mean_circ: 0.939357
Ran for 81550 frames Cell Events: 2969 mean_circ: 0.939439
Ran for 82050 frames Cell Events: 2976 mean_circ: 0.939451
Ran for 82550 frames Cell Events: 2997 mean_circ: 0.939437
Ran for 83050 frames Cell Events: 3022 mean_circ: 0.939476
Ran for 83550 frames Cell Events: 3052 mean_circ: 0.939279
Ran for 84050 frames Cell Events: 3065 mean_circ: 0.939363
Ran for 84550 frames Cell Events: 3080 mean_circ: 0.93939
Ran for 85050 frames Cell Events: 3093 mean_circ: 0.939419
Ran for 85550 frames Cell Events: 3116 mean_circ: 0.939352
Ran for 86050 frames Cell Events: 3122 mean_circ: 0.93931
Ran for 87050 frames Cell Events: 3154 mean_circ: 0.939309
Ran for 88550 frames Cell Events: 3211 mean_circ: 0.939457
Ran for 89050 frames Cell Events: 3227 mean_circ: 0.939482
Ran for 90050 frames Cell Events: 3261 mean_circ: 0.939466
Ran for 90550 frames Cell Events: 3285 mean_circ: 0.939451
Ran for 92050 frames Cell Events: 3335 mean_circ: 0.939373
Ran for 93050 frames Cell Events: 3385 mean_circ: 0.939383
Ran for 94050 frames Cell Events: 3423 mean_circ: 0.939319
Ran for 94550 frames Cell Events: 3450 mean_circ: 0.939344
Ran for 95050 frames Cell Events: 3466 mean_circ: 0.939367
Ran for 95550 frames Cell Events: 3483 mean_circ: 0.9393
Ran for 96050 frames Cell Events: 3494 mean_circ: 0.939285
Ran for 96550 frames Cell Events: 3511 mean_circ: 0.939191
Ran for 98050 frames Cell Events: 3576 mean_circ: 0.939216
Ran for 98550 frames Cell Events: 3591 mean_circ: 0.939127
Ran for 99050 frames Cell Events: 3618 mean_circ: 0.93916
Ran for 99550 frames Cell Events: 3635 mean_circ: 0.939152
Ran for 100550 frames Cell Events: 3673 mean_circ: 0.939197
Ran for 101050 frames Cell Events: 3686 mean_circ: 0.939232
Ran for 102050 frames Cell Events: 3731 mean_circ: 0.93925
Ran for 104050 frames Cell Events: 3804 mean_circ: 0.939298
Ran for 105050 frames Cell Events: 3834 mean_circ: 0.939356
Ran for 105550 frames Cell Events: 3849 mean_circ: 0.939375
Ran for 106050 frames Cell Events: 3868 mean_circ: 0.939322
Ran for 106550 frames Cell Events: 3885 mean_circ: 0.939301
Ran for 108050 frames Cell Events: 3957 mean_circ: 0.939287
Ran for 108550 frames Cell Events: 3963 mean_circ: 0.939247
Ran for 109550 frames Cell Events: 3995 mean_circ: 0.939218
Ran for 112050 frames Cell Events: 4107 mean_circ: 0.939252
Ran for 112550 frames Cell Events: 4126 mean_circ: 0.939294
Ran for 113050 frames Cell Events: 4146 mean_circ: 0.939289
Ran for 114050 frames Cell Events: 4182 mean_circ: 0.939269
Ran for 115550 frames Cell Events: 4249 mean_circ: 0.939221
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Ran for 118050 frames Cell Events: 4368 mean_circ: 0.939337
Ran for 118550 frames Cell Events: 4404 mean_circ: 0.939337
Ran for 119550 frames Cell Events: 4453 mean_circ: 0.93925
Ran for 120050 frames Cell Events: 4465 mean_circ: 0.939288
Ran for 122550 frames Cell Events: 4567 mean_circ: 0.939319
Ran for 124050 frames Cell Events: 4633 mean_circ: 0.93926
Ran for 125550 frames Cell Events: 4698 mean_circ: 0.939259
Ran for 126050 frames Cell Events: 4718 mean_circ: 0.939224
Ran for 126550 frames Cell Events: 4730 mean_circ: 0.939266
Ran for 127050 frames Cell Events: 4742 mean_circ: 0.939247
Ran for 129050 frames Cell Events: 4823 mean_circ: 0.939206
Ran for 130050 frames Cell Events: 4860 mean_circ: 0.939154
Ran for 130550 frames Cell Events: 4875 mean_circ: 0.939148
Ran for 131050 frames Cell Events: 4886 mean_circ: 0.939159
Ran for 131550 frames Cell Events: 4906 mean_circ: 0.939148
Ran for 132050 frames Cell Events: 4922 mean_circ: 0.939065
Ran for 132550 frames Cell Events: 4936 mean_circ: 0.939041
Ran for 133550 frames Cell Events: 4964 mean_circ: 0.938959
Ran for 134050 frames Cell Events: 4978 mean_circ: 0.938946
Ran for 135050 frames Cell Events: 5008 mean_circ: 0.939021
Ran for 136550 frames Cell Events: 5074 mean_circ: 0.939109
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Ran for 140050 frames Cell Events: 5192 mean_circ: 0.939047
Ran for 140550 frames Cell Events: 5206 mean_circ: 0.939059
Ran for 141050 frames Cell Events: 5225 mean_circ: 0.939063
Ran for 141550 frames Cell Events: 5237 mean_circ: 0.93906
Ran for 142050 frames Cell Events: 5251 mean_circ: 0.939025
Ran for 143050 frames Cell Events: 5284 mean_circ: 0.938922
Ran for 143550 frames Cell Events: 5309 mean_circ: 0.938932
Ran for 144050 frames Cell Events: 5329 mean_circ: 0.939001
Ran for 145050 frames Cell Events: 5368 mean_circ: 0.938966
Ran for 145550 frames Cell Events: 5384 mean_circ: 0.93898
Ran for 146050 frames Cell Events: 5403 mean_circ: 0.938946
Ran for 146550 frames Cell Events: 5425 mean_circ: 0.938952
Ran for 148050 frames Cell Events: 5489 mean_circ: 0.938954
Ran for 149050 frames Cell Events: 5531 mean_circ: 0.938994
Ran for 149550 frames Cell Events: 5554 mean_circ: 0.939045
Ran for 150050 frames Cell Events: 5580 mean_circ: 0.939053
Ran for 151050 frames Cell Events: 5608 mean_circ: 0.939056
Ran for 152050 frames Cell Events: 5644 mean_circ: 0.939002
Ran for 152550 frames Cell Events: 5671 mean_circ: 0.939036
Ran for 153050 frames Cell Events: 5691 mean_circ: 0.938999
Ran for 154050 frames Cell Events: 5728 mean_circ: 0.93891
Ran for 155550 frames Cell Events: 5787 mean_circ: 0.93893
Ran for 156050 frames Cell Events: 5806 mean_circ: 0.938908
Ran for 156550 frames Cell Events: 5824 mean_circ: 0.938943
Ran for 157050 frames Cell Events: 5842 mean_circ: 0.938893
Ran for 157550 frames Cell Events: 5856 mean_circ: 0.938896
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Ran for 159050 frames Cell Events: 5897 mean_circ: 0.938941
Ran for 161050 frames Cell Events: 5984 mean_circ: 0.938856
Ran for 161550 frames Cell Events: 6007 mean_circ: 0.938903
Ran for 162050 frames Cell Events: 6024 mean_circ: 0.938929
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Ran for 172050 frames Cell Events: 6448 mean_circ: 0.93888
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Ran for 180550 frames Cell Events: 6836 mean_circ: 0.938902
Ran for 182550 frames Cell Events: 6913 mean_circ: 0.938951
Ran for 184550 frames Cell Events: 6990 mean_circ: 0.938866
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Ran for 190050 frames Cell Events: 7211 mean_circ: 0.938947
Ran for 190550 frames Cell Events: 7234 mean_circ: 0.938962
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Ran for 191550 frames Cell Events: 7289 mean_circ: 0.938975
Ran for 193550 frames Cell Events: 7379 mean_circ: 0.938897
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Ran for 200550 frames Cell Events: 7687 mean_circ: 0.938879
Ran for 201050 frames Cell Events: 7709 mean_circ: 0.938858
Ran for 201550 frames Cell Events: 7721 mean_circ: 0.938888
Ran for 202050 frames Cell Events: 7748 mean_circ: 0.938896
Ran for 202550 frames Cell Events: 7762 mean_circ: 0.938931
Ran for 203050 frames Cell Events: 7780 mean_circ: 0.93893
Ran for 203550 frames Cell Events: 7801 mean_circ: 0.938921
Ran for 204050 frames Cell Events: 7837 mean_circ: 0.938882
Ran for 204550 frames Cell Events: 7864 mean_circ: 0.938922
Ran for 206050 frames Cell Events: 7928 mean_circ: 0.938921
Ran for 206550 frames Cell Events: 7954 mean_circ: 0.938956
Ran for 207550 frames Cell Events: 7988 mean_circ: 0.938976
Ran for 208050 frames Cell Events: 8004 mean_circ: 0.939004
Ran for 208550 frames Cell Events: 8020 mean_circ: 0.938988
Ran for 209050 frames Cell Events: 8037 mean_circ: 0.939016
Ran for 209550 frames Cell Events: 8046 mean_circ: 0.939034
End this measurment..
Stopping time: 2.1113e+009
****************************************
Measurement finished
Mean Circularitiy: 0.939028
Mean Area: 678.5
Mean FL-1 intensity: 4430.14
Mean FL-2 intensity: 6010.23
Mean FL-3 intensity: 0
frames processed: 208978
Total Time: 104908ms
Number of Runs (new bg img) 0
Frames collected: 209676
Skipped frames: 15712
Rescued Frames: 1446035
Samples DAQ: 52419706
Samples written: 104837746
Samples per Channel: 52418873
FPS: 1998.66
Samples per second: 499664
Expected samples/s: 500000
Events: 8054
Events per second: 76.7718
jumped back (PMT): 9
#Frames read, frequency
# 0 frames :306727
# 1 frames :96433
# 2 frames :4289
# 3 frames :1574
# 4 frames :926
# 5 frames :500
# 6 frames :1202
# 7 frames :1946
# 8 frames :997
# 9 frames :802
# 10 frames :820
# 11 frames :541
# 12 frames :387
# 13 frames :296
# 14 frames :189
# 15 frames :207
# 16 frames :228
# 17 frames :232
# 18 frames :207
# 19 frames :131
# 20 frames :124
# 21 frames :130
# 22 frames :86
# 23 frames :47
# 24 frames :37
# 25 frames :38
# 26 frames :26
# 27 frames :22
# 28 frames :21
# 29 frames :15
# 30 frames :16
# 31 frames :10
# 32 frames :7
# 33 frames :4
# 34 frames :5
# 35 frames :4
# 36 frames :5
# 37 frames :1
# 38 frames :4
# 39 frames :4
# 40 frames :1
# 41 frames :1
# 42 frames :6
# 43 frames :1
# 46 frames :1
# 47 frames :1
# 48 frames :1
# 54 frames :1
# 63 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9608
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0