M1_20170608_1933_c2_V3_faster.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.38.40
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3f8e9145fc2f8db557f730a9e5e48512",
"name": "M1_20170608_1933_c2_V3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:39:00"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarningin dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7255 (max 7256) in '7255'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0481
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.11381e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 2 mean_circ: 0.910757
Ran for 550 frames Cell Events: 4 mean_circ: 0.950273
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Ran for 411550 frames Cell Events: 5436 mean_circ: 0.896289
Ran for 412550 frames Cell Events: 5450 mean_circ: 0.896276
Ran for 413050 frames Cell Events: 5459 mean_circ: 0.896274
Ran for 413550 frames Cell Events: 5465 mean_circ: 0.896273
Ran for 414050 frames Cell Events: 5477 mean_circ: 0.896285
Ran for 414550 frames Cell Events: 5487 mean_circ: 0.89632
Ran for 415050 frames Cell Events: 5506 mean_circ: 0.896307
Ran for 415550 frames Cell Events: 5512 mean_circ: 0.896347
Ran for 416050 frames Cell Events: 5515 mean_circ: 0.89633
Ran for 416550 frames Cell Events: 5520 mean_circ: 0.896351
Ran for 417050 frames Cell Events: 5533 mean_circ: 0.896352
Ran for 417550 frames Cell Events: 5545 mean_circ: 0.896371
Ran for 418050 frames Cell Events: 5554 mean_circ: 0.896387
Ran for 418550 frames Cell Events: 5557 mean_circ: 0.896356
Ran for 419050 frames Cell Events: 5565 mean_circ: 0.896353
Ran for 419550 frames Cell Events: 5571 mean_circ: 0.896337
Ran for 420550 frames Cell Events: 5588 mean_circ: 0.896392
Ran for 423550 frames Cell Events: 5624 mean_circ: 0.896379
Ran for 424050 frames Cell Events: 5627 mean_circ: 0.89638
Ran for 425050 frames Cell Events: 5640 mean_circ: 0.89636
Ran for 425550 frames Cell Events: 5645 mean_circ: 0.896353
Ran for 426050 frames Cell Events: 5649 mean_circ: 0.896338
Ran for 426550 frames Cell Events: 5652 mean_circ: 0.896366
Ran for 427550 frames Cell Events: 5669 mean_circ: 0.896281
Ran for 428050 frames Cell Events: 5675 mean_circ: 0.896262
Ran for 428550 frames Cell Events: 5678 mean_circ: 0.896253
Ran for 429050 frames Cell Events: 5685 mean_circ: 0.89624
Ran for 429550 frames Cell Events: 5686 mean_circ: 0.896221
Ran for 430050 frames Cell Events: 5692 mean_circ: 0.896211
Ran for 430550 frames Cell Events: 5694 mean_circ: 0.896222
Ran for 431050 frames Cell Events: 5699 mean_circ: 0.896201
Ran for 432050 frames Cell Events: 5721 mean_circ: 0.896281
Ran for 432550 frames Cell Events: 5733 mean_circ: 0.896273
Ran for 433050 frames Cell Events: 5736 mean_circ: 0.896248
Ran for 433550 frames Cell Events: 5737 mean_circ: 0.896236
Ran for 434050 frames Cell Events: 5743 mean_circ: 0.896188
Ran for 434550 frames Cell Events: 5745 mean_circ: 0.896207
Ran for 435050 frames Cell Events: 5755 mean_circ: 0.896225
Ran for 435550 frames Cell Events: 5760 mean_circ: 0.896206
Ran for 436050 frames Cell Events: 5767 mean_circ: 0.896224
Ran for 436550 frames Cell Events: 5773 mean_circ: 0.896184
Ran for 437550 frames Cell Events: 5793 mean_circ: 0.896186
Ran for 438050 frames Cell Events: 5796 mean_circ: 0.896196
Ran for 438550 frames Cell Events: 5805 mean_circ: 0.896196
Ran for 439050 frames Cell Events: 5811 mean_circ: 0.896187
Ran for 439550 frames Cell Events: 5815 mean_circ: 0.896209
Ran for 440050 frames Cell Events: 5826 mean_circ: 0.896187
Ran for 440550 frames Cell Events: 5830 mean_circ: 0.896153
Ran for 441050 frames Cell Events: 5838 mean_circ: 0.8962
Ran for 441550 frames Cell Events: 5848 mean_circ: 0.896159
Ran for 442050 frames Cell Events: 5854 mean_circ: 0.896126
Ran for 442550 frames Cell Events: 5857 mean_circ: 0.89609
Ran for 443050 frames Cell Events: 5863 mean_circ: 0.896126
Ran for 443550 frames Cell Events: 5867 mean_circ: 0.896126
Ran for 444050 frames Cell Events: 5868 mean_circ: 0.896125
Ran for 444550 frames Cell Events: 5869 mean_circ: 0.896129
Ran for 445050 frames Cell Events: 5870 mean_circ: 0.896131
Ran for 445550 frames Cell Events: 5875 mean_circ: 0.896116
Ran for 446050 frames Cell Events: 5880 mean_circ: 0.896083
Ran for 446550 frames Cell Events: 5888 mean_circ: 0.896002
Ran for 447550 frames Cell Events: 5909 mean_circ: 0.895954
Ran for 448050 frames Cell Events: 5919 mean_circ: 0.895964
Ran for 448550 frames Cell Events: 5922 mean_circ: 0.895981
Ran for 449550 frames Cell Events: 5938 mean_circ: 0.895989
Ran for 450050 frames Cell Events: 5944 mean_circ: 0.895991
Ran for 451550 frames Cell Events: 5972 mean_circ: 0.895849
Ran for 452550 frames Cell Events: 5988 mean_circ: 0.895849
Ran for 453050 frames Cell Events: 5993 mean_circ: 0.895788
Ran for 453550 frames Cell Events: 5998 mean_circ: 0.895801
Ran for 454050 frames Cell Events: 6000 mean_circ: 0.895803
Ran for 454550 frames Cell Events: 6011 mean_circ: 0.895832
Ran for 455050 frames Cell Events: 6018 mean_circ: 0.895827
Ran for 456550 frames Cell Events: 6039 mean_circ: 0.895878
Ran for 457050 frames Cell Events: 6057 mean_circ: 0.895924
Ran for 458050 frames Cell Events: 6073 mean_circ: 0.895937
Ran for 458550 frames Cell Events: 6079 mean_circ: 0.895928
Ran for 459550 frames Cell Events: 6094 mean_circ: 0.895905
Ran for 460050 frames Cell Events: 6106 mean_circ: 0.895895
Ran for 460550 frames Cell Events: 6108 mean_circ: 0.895889
Ran for 461050 frames Cell Events: 6112 mean_circ: 0.895889
Ran for 462550 frames Cell Events: 6130 mean_circ: 0.895898
Ran for 463050 frames Cell Events: 6142 mean_circ: 0.895818
Ran for 463550 frames Cell Events: 6154 mean_circ: 0.895836
Ran for 464050 frames Cell Events: 6162 mean_circ: 0.895841
Ran for 464550 frames Cell Events: 6165 mean_circ: 0.89584
Ran for 465050 frames Cell Events: 6168 mean_circ: 0.895839
Ran for 466050 frames Cell Events: 6177 mean_circ: 0.895863
Ran for 466550 frames Cell Events: 6187 mean_circ: 0.895841
Ran for 467050 frames Cell Events: 6191 mean_circ: 0.89586
Ran for 467550 frames Cell Events: 6193 mean_circ: 0.895847
Ran for 468550 frames Cell Events: 6206 mean_circ: 0.895845
Ran for 469050 frames Cell Events: 6210 mean_circ: 0.895865
Ran for 469550 frames Cell Events: 6215 mean_circ: 0.895839
Ran for 470050 frames Cell Events: 6221 mean_circ: 0.895836
Ran for 470550 frames Cell Events: 6231 mean_circ: 0.895839
Ran for 471050 frames Cell Events: 6239 mean_circ: 0.895849
Ran for 472050 frames Cell Events: 6260 mean_circ: 0.895906
Ran for 473550 frames Cell Events: 6271 mean_circ: 0.895849
Ran for 475050 frames Cell Events: 6289 mean_circ: 0.895863
Ran for 476050 frames Cell Events: 6308 mean_circ: 0.895897
Ran for 476550 frames Cell Events: 6316 mean_circ: 0.895926
Ran for 477050 frames Cell Events: 6319 mean_circ: 0.895942
Ran for 478050 frames Cell Events: 6330 mean_circ: 0.896006
Ran for 478550 frames Cell Events: 6337 mean_circ: 0.895998
Ran for 479550 frames Cell Events: 6356 mean_circ: 0.896013
Ran for 480050 frames Cell Events: 6362 mean_circ: 0.89598
Ran for 480550 frames Cell Events: 6370 mean_circ: 0.895897
Ran for 481050 frames Cell Events: 6382 mean_circ: 0.895916
Ran for 482550 frames Cell Events: 6423 mean_circ: 0.89581
Ran for 483550 frames Cell Events: 6439 mean_circ: 0.895834
Ran for 484050 frames Cell Events: 6442 mean_circ: 0.89583
Ran for 484550 frames Cell Events: 6447 mean_circ: 0.895839
Ran for 485050 frames Cell Events: 6459 mean_circ: 0.895833
Ran for 485550 frames Cell Events: 6468 mean_circ: 0.895861
Ran for 486050 frames Cell Events: 6478 mean_circ: 0.89588
Ran for 486550 frames Cell Events: 6483 mean_circ: 0.895908
Ran for 487550 frames Cell Events: 6503 mean_circ: 0.895916
Ran for 488550 frames Cell Events: 6522 mean_circ: 0.895937
Ran for 489050 frames Cell Events: 6529 mean_circ: 0.895948
Ran for 490550 frames Cell Events: 6546 mean_circ: 0.895899
Ran for 491050 frames Cell Events: 6552 mean_circ: 0.895925
Ran for 492050 frames Cell Events: 6564 mean_circ: 0.895921
Ran for 492550 frames Cell Events: 6576 mean_circ: 0.895933
Ran for 493050 frames Cell Events: 6580 mean_circ: 0.89595
Ran for 493550 frames Cell Events: 6591 mean_circ: 0.895932
Ran for 494050 frames Cell Events: 6599 mean_circ: 0.895935
Ran for 494550 frames Cell Events: 6606 mean_circ: 0.895904
Ran for 495050 frames Cell Events: 6624 mean_circ: 0.895867
Ran for 496050 frames Cell Events: 6647 mean_circ: 0.895902
Ran for 496550 frames Cell Events: 6654 mean_circ: 0.895955
Ran for 497050 frames Cell Events: 6662 mean_circ: 0.895957
Ran for 500050 frames Cell Events: 6768 mean_circ: 0.89623
Ran for 500550 frames Cell Events: 6773 mean_circ: 0.896246
Ran for 501050 frames Cell Events: 6786 mean_circ: 0.89624
Ran for 501550 frames Cell Events: 6791 mean_circ: 0.89621
Ran for 502050 frames Cell Events: 6801 mean_circ: 0.896224
Ran for 502550 frames Cell Events: 6806 mean_circ: 0.896242
Ran for 503050 frames Cell Events: 6809 mean_circ: 0.896215
Ran for 504050 frames Cell Events: 6825 mean_circ: 0.896229
Ran for 504550 frames Cell Events: 6831 mean_circ: 0.896256
Ran for 505550 frames Cell Events: 6844 mean_circ: 0.896268
Ran for 506050 frames Cell Events: 6855 mean_circ: 0.89629
Ran for 506550 frames Cell Events: 6866 mean_circ: 0.896303
Ran for 507050 frames Cell Events: 6869 mean_circ: 0.896302
Ran for 507550 frames Cell Events: 6873 mean_circ: 0.896326
Ran for 508050 frames Cell Events: 6881 mean_circ: 0.896316
Ran for 508550 frames Cell Events: 6890 mean_circ: 0.896366
Ran for 509050 frames Cell Events: 6892 mean_circ: 0.896382
Ran for 509550 frames Cell Events: 6897 mean_circ: 0.896378
Ran for 510050 frames Cell Events: 6908 mean_circ: 0.896363
Ran for 510550 frames Cell Events: 6913 mean_circ: 0.89637
Ran for 511050 frames Cell Events: 6924 mean_circ: 0.896438
Ran for 511550 frames Cell Events: 6931 mean_circ: 0.896438
Ran for 513550 frames Cell Events: 6950 mean_circ: 0.896296
Ran for 514050 frames Cell Events: 6955 mean_circ: 0.896275
Ran for 514550 frames Cell Events: 6965 mean_circ: 0.896275
Ran for 515050 frames Cell Events: 6969 mean_circ: 0.896304
Ran for 515550 frames Cell Events: 6977 mean_circ: 0.896309
Ran for 516050 frames Cell Events: 6986 mean_circ: 0.896326
Ran for 517050 frames Cell Events: 7015 mean_circ: 0.896333
Ran for 517550 frames Cell Events: 7025 mean_circ: 0.896339
Ran for 518550 frames Cell Events: 7037 mean_circ: 0.89629
Ran for 519050 frames Cell Events: 7047 mean_circ: 0.896301
Ran for 520050 frames Cell Events: 7056 mean_circ: 0.896279
Ran for 520550 frames Cell Events: 7062 mean_circ: 0.896228
Ran for 521050 frames Cell Events: 7068 mean_circ: 0.896224
Ran for 521550 frames Cell Events: 7071 mean_circ: 0.896228
Ran for 522050 frames Cell Events: 7084 mean_circ: 0.896209
Ran for 522550 frames Cell Events: 7089 mean_circ: 0.896207
Ran for 523550 frames Cell Events: 7106 mean_circ: 0.896265
Ran for 524550 frames Cell Events: 7120 mean_circ: 0.896278
Ran for 525050 frames Cell Events: 7126 mean_circ: 0.896306
Ran for 525550 frames Cell Events: 7133 mean_circ: 0.896294
Ran for 526050 frames Cell Events: 7139 mean_circ: 0.896285
Ran for 527050 frames Cell Events: 7148 mean_circ: 0.896304
Ran for 528050 frames Cell Events: 7161 mean_circ: 0.896308
Ran for 528550 frames Cell Events: 7168 mean_circ: 0.896284
Ran for 529050 frames Cell Events: 7169 mean_circ: 0.896279
Ran for 529550 frames Cell Events: 7175 mean_circ: 0.896248
Ran for 530550 frames Cell Events: 7200 mean_circ: 0.896263
Ran for 531050 frames Cell Events: 7212 mean_circ: 0.896245
Ran for 531550 frames Cell Events: 7219 mean_circ: 0.896261
Ran for 532050 frames Cell Events: 7225 mean_circ: 0.896229
Ran for 532550 frames Cell Events: 7234 mean_circ: 0.896224
Ran for 533050 frames Cell Events: 7243 mean_circ: 0.896246
Ran for 533550 frames Cell Events: 7247 mean_circ: 0.896222
Ran for 534050 frames Cell Events: 7252 mean_circ: 0.896227
Ran for 534550 frames Cell Events: 7256 mean_circ: 0.896227
End this measurment..
Stopping time: 2.11408e+009
****************************************
Measurement finished
Mean Circularitiy: 0.896233
Mean Area: 1505.28
Mean FL-1 intensity: 7211.67
Mean FL-2 intensity: 8976.91
Mean FL-3 intensity: 0
frames processed: 533862
Total Time: 267329ms
Number of Runs (new bg img) 0
Frames collected: 534570
Skipped frames: 26852
Rescued Frames: 2363757
Samples DAQ: 133642745
Samples written: 267285054
Samples per Channel: 133642527
FPS: 1999.67
Samples per second: 499917
Expected samples/s: 500000
Events: 7256
Events per second: 27.1426
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :730511
# 1 frames :309839
# 2 frames :6357
# 3 frames :3605
# 4 frames :2579
# 5 frames :1879
# 6 frames :1219
# 7 frames :1216
# 8 frames :1389
# 9 frames :1178
# 10 frames :1100
# 11 frames :1124
# 12 frames :1216
# 13 frames :941
# 14 frames :667
# 15 frames :464
# 16 frames :406
# 17 frames :368
# 18 frames :452
# 19 frames :533
# 20 frames :459
# 21 frames :396
# 22 frames :309
# 23 frames :222
# 24 frames :148
# 25 frames :89
# 26 frames :58
# 27 frames :47
# 28 frames :35
# 29 frames :24
# 30 frames :24
# 31 frames :23
# 32 frames :21
# 33 frames :20
# 34 frames :12
# 35 frames :10
# 36 frames :12
# 37 frames :7
# 38 frames :7
# 39 frames :2
# 40 frames :4
# 41 frames :6
# 42 frames :7
# 43 frames :5
# 44 frames :5
# 45 frames :2
# 46 frames :4
# 48 frames :3
# 52 frames :1
# 54 frames :1
# 55 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0481
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 5fe6b70027a55b46569a1656e683826c |
format | RT-FDC |
id | ca540645-22b3-4f7a-bda5-8a3694fbe8d4 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | 3fd734ab-cfb7-4934-a513-6d09a8f51e0b |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/ca5/406/45-22b3-4f7a-bda5-8a3694fbe8d4 |
sha256 | 5415673bd09c8cbac67d1f67be4b2de07e69b11da7c8372a459ec60e2869fcd3 |
size | 130.3 MiB |
dc:experiment:date | 2017-06-08 |
dc:experiment:event count | 7,254 |
dc:experiment:run identifier | None-6bb9 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170608_1933_c2_V3_faster |
dc:experiment:time | 19:33:21 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 200 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.06 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |