Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.47.22
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3c4a6939a1399714063feadf29c9b139",
"name": "M1_20170608_2121_c2_nk2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:47:43"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8256 (max 8257) in '8256'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1528
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.12031e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 38 mean_circ: 0.938255
Ran for 1550 frames Cell Events: 46 mean_circ: 0.937551
Ran for 2550 frames Cell Events: 79 mean_circ: 0.940743
Ran for 3550 frames Cell Events: 112 mean_circ: 0.9411
Ran for 4050 frames Cell Events: 124 mean_circ: 0.941304
Ran for 4550 frames Cell Events: 139 mean_circ: 0.942365
Ran for 5550 frames Cell Events: 180 mean_circ: 0.941376
Ran for 6050 frames Cell Events: 192 mean_circ: 0.942196
Ran for 8050 frames Cell Events: 279 mean_circ: 0.941911
Ran for 8550 frames Cell Events: 308 mean_circ: 0.942139
Ran for 9550 frames Cell Events: 359 mean_circ: 0.943604
Ran for 10550 frames Cell Events: 390 mean_circ: 0.943692
Ran for 11050 frames Cell Events: 403 mean_circ: 0.943526
Ran for 12050 frames Cell Events: 430 mean_circ: 0.944182
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Ran for 13050 frames Cell Events: 453 mean_circ: 0.944115
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Ran for 16050 frames Cell Events: 545 mean_circ: 0.943569
Ran for 16550 frames Cell Events: 559 mean_circ: 0.943558
Ran for 18050 frames Cell Events: 608 mean_circ: 0.943102
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Ran for 21050 frames Cell Events: 684 mean_circ: 0.942498
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Ran for 30550 frames Cell Events: 974 mean_circ: 0.940475
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Ran for 78550 frames Cell Events: 2349 mean_circ: 0.941325
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Ran for 81050 frames Cell Events: 2411 mean_circ: 0.941467
Ran for 81550 frames Cell Events: 2422 mean_circ: 0.941485
Ran for 82550 frames Cell Events: 2450 mean_circ: 0.941528
Ran for 84050 frames Cell Events: 2498 mean_circ: 0.941614
Ran for 85050 frames Cell Events: 2536 mean_circ: 0.941597
Ran for 85550 frames Cell Events: 2539 mean_circ: 0.941631
Ran for 86050 frames Cell Events: 2551 mean_circ: 0.941663
Ran for 86550 frames Cell Events: 2566 mean_circ: 0.94163
Ran for 87550 frames Cell Events: 2597 mean_circ: 0.941783
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Ran for 92550 frames Cell Events: 2755 mean_circ: 0.941607
Ran for 93050 frames Cell Events: 2761 mean_circ: 0.941621
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Ran for 96050 frames Cell Events: 2863 mean_circ: 0.941535
Ran for 98050 frames Cell Events: 2911 mean_circ: 0.941294
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Ran for 100050 frames Cell Events: 2981 mean_circ: 0.941421
Ran for 100550 frames Cell Events: 2996 mean_circ: 0.941442
Ran for 101050 frames Cell Events: 3006 mean_circ: 0.941463
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Ran for 102550 frames Cell Events: 3055 mean_circ: 0.941418
Ran for 103550 frames Cell Events: 3088 mean_circ: 0.94143
Ran for 104050 frames Cell Events: 3100 mean_circ: 0.941488
Ran for 104550 frames Cell Events: 3120 mean_circ: 0.941332
Ran for 105050 frames Cell Events: 3133 mean_circ: 0.941281
Ran for 105550 frames Cell Events: 3142 mean_circ: 0.941332
Ran for 106550 frames Cell Events: 3172 mean_circ: 0.941258
Ran for 107550 frames Cell Events: 3197 mean_circ: 0.941085
Ran for 109050 frames Cell Events: 3237 mean_circ: 0.94111
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Ran for 110550 frames Cell Events: 3277 mean_circ: 0.941096
Ran for 111550 frames Cell Events: 3309 mean_circ: 0.941066
Ran for 112550 frames Cell Events: 3343 mean_circ: 0.941111
Ran for 113550 frames Cell Events: 3370 mean_circ: 0.941032
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Ran for 114550 frames Cell Events: 3410 mean_circ: 0.941022
Ran for 115050 frames Cell Events: 3431 mean_circ: 0.940834
Ran for 116050 frames Cell Events: 3450 mean_circ: 0.940777
Ran for 116550 frames Cell Events: 3458 mean_circ: 0.940817
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Ran for 118550 frames Cell Events: 3512 mean_circ: 0.940676
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Ran for 121550 frames Cell Events: 3612 mean_circ: 0.940701
Ran for 122050 frames Cell Events: 3624 mean_circ: 0.940718
Ran for 123050 frames Cell Events: 3648 mean_circ: 0.940786
Ran for 124050 frames Cell Events: 3679 mean_circ: 0.940812
Ran for 124550 frames Cell Events: 3689 mean_circ: 0.940786
Ran for 125550 frames Cell Events: 3719 mean_circ: 0.940754
Ran for 126550 frames Cell Events: 3748 mean_circ: 0.940734
Ran for 127050 frames Cell Events: 3763 mean_circ: 0.940691
Ran for 128050 frames Cell Events: 3791 mean_circ: 0.940694
Ran for 128550 frames Cell Events: 3801 mean_circ: 0.940672
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Ran for 131050 frames Cell Events: 3860 mean_circ: 0.940682
Ran for 132050 frames Cell Events: 3881 mean_circ: 0.940744
Ran for 132550 frames Cell Events: 3894 mean_circ: 0.940807
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Ran for 141050 frames Cell Events: 4158 mean_circ: 0.94074
Ran for 141550 frames Cell Events: 4176 mean_circ: 0.940716
Ran for 142550 frames Cell Events: 4200 mean_circ: 0.940694
Ran for 143550 frames Cell Events: 4225 mean_circ: 0.94062
Ran for 144550 frames Cell Events: 4265 mean_circ: 0.940671
Ran for 145550 frames Cell Events: 4297 mean_circ: 0.940774
Ran for 146050 frames Cell Events: 4312 mean_circ: 0.940745
Ran for 147050 frames Cell Events: 4335 mean_circ: 0.940723
Ran for 147550 frames Cell Events: 4347 mean_circ: 0.940708
Ran for 148050 frames Cell Events: 4360 mean_circ: 0.940646
Ran for 149050 frames Cell Events: 4395 mean_circ: 0.940655
Ran for 150050 frames Cell Events: 4431 mean_circ: 0.940629
Ran for 150550 frames Cell Events: 4445 mean_circ: 0.940567
Ran for 155050 frames Cell Events: 4602 mean_circ: 0.940541
Ran for 156050 frames Cell Events: 4645 mean_circ: 0.940581
Ran for 156550 frames Cell Events: 4666 mean_circ: 0.940609
Ran for 157050 frames Cell Events: 4679 mean_circ: 0.940587
Ran for 157550 frames Cell Events: 4690 mean_circ: 0.940582
Ran for 158550 frames Cell Events: 4723 mean_circ: 0.940425
Ran for 162050 frames Cell Events: 4832 mean_circ: 0.94051
Ran for 162550 frames Cell Events: 4848 mean_circ: 0.940564
Ran for 163050 frames Cell Events: 4860 mean_circ: 0.940596
Ran for 164050 frames Cell Events: 4902 mean_circ: 0.940601
Ran for 165050 frames Cell Events: 4941 mean_circ: 0.94064
Ran for 165550 frames Cell Events: 4956 mean_circ: 0.940634
Ran for 166550 frames Cell Events: 4988 mean_circ: 0.940713
Ran for 167550 frames Cell Events: 5012 mean_circ: 0.9407
Ran for 168050 frames Cell Events: 5032 mean_circ: 0.940651
Ran for 168550 frames Cell Events: 5043 mean_circ: 0.940627
Ran for 169050 frames Cell Events: 5057 mean_circ: 0.940661
Ran for 171550 frames Cell Events: 5147 mean_circ: 0.940629
Ran for 172050 frames Cell Events: 5162 mean_circ: 0.94067
Ran for 172550 frames Cell Events: 5172 mean_circ: 0.940658
Ran for 173550 frames Cell Events: 5203 mean_circ: 0.940679
Ran for 175050 frames Cell Events: 5264 mean_circ: 0.940738
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Ran for 176050 frames Cell Events: 5294 mean_circ: 0.940669
Ran for 176550 frames Cell Events: 5310 mean_circ: 0.94066
Ran for 177550 frames Cell Events: 5360 mean_circ: 0.9406
Ran for 178050 frames Cell Events: 5375 mean_circ: 0.940586
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Ran for 182050 frames Cell Events: 5497 mean_circ: 0.940507
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Ran for 204050 frames Cell Events: 6206 mean_circ: 0.940734
Ran for 206050 frames Cell Events: 6287 mean_circ: 0.940741
Ran for 206550 frames Cell Events: 6304 mean_circ: 0.940717
Ran for 208050 frames Cell Events: 6350 mean_circ: 0.940753
Ran for 209550 frames Cell Events: 6413 mean_circ: 0.940758
Ran for 211050 frames Cell Events: 6459 mean_circ: 0.940812
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Ran for 221050 frames Cell Events: 6770 mean_circ: 0.940788
Ran for 222050 frames Cell Events: 6786 mean_circ: 0.940805
Ran for 222550 frames Cell Events: 6803 mean_circ: 0.940799
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Ran for 226050 frames Cell Events: 6913 mean_circ: 0.940677
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Ran for 236550 frames Cell Events: 7260 mean_circ: 0.940665
Ran for 237550 frames Cell Events: 7293 mean_circ: 0.94061
Ran for 238050 frames Cell Events: 7313 mean_circ: 0.94061
Ran for 239050 frames Cell Events: 7351 mean_circ: 0.940655
Ran for 239550 frames Cell Events: 7360 mean_circ: 0.940667
Ran for 240050 frames Cell Events: 7370 mean_circ: 0.94069
Ran for 240550 frames Cell Events: 7394 mean_circ: 0.940682
Ran for 241050 frames Cell Events: 7408 mean_circ: 0.940669
Ran for 241550 frames Cell Events: 7432 mean_circ: 0.940636
Ran for 242050 frames Cell Events: 7453 mean_circ: 0.940637
Ran for 242550 frames Cell Events: 7471 mean_circ: 0.94064
Ran for 243050 frames Cell Events: 7486 mean_circ: 0.940665
Ran for 244050 frames Cell Events: 7514 mean_circ: 0.940685
Ran for 245050 frames Cell Events: 7542 mean_circ: 0.940689
Ran for 245550 frames Cell Events: 7557 mean_circ: 0.940717
Ran for 247050 frames Cell Events: 7604 mean_circ: 0.940688
Ran for 249550 frames Cell Events: 7689 mean_circ: 0.940769
Ran for 250050 frames Cell Events: 7706 mean_circ: 0.940743
Ran for 250550 frames Cell Events: 7724 mean_circ: 0.940767
Ran for 252050 frames Cell Events: 7774 mean_circ: 0.940692
Ran for 252550 frames Cell Events: 7791 mean_circ: 0.940711
Ran for 253050 frames Cell Events: 7810 mean_circ: 0.940701
Ran for 253550 frames Cell Events: 7827 mean_circ: 0.940709
Ran for 254050 frames Cell Events: 7843 mean_circ: 0.940727
Ran for 254550 frames Cell Events: 7857 mean_circ: 0.940706
Ran for 255050 frames Cell Events: 7861 mean_circ: 0.940683
Ran for 255550 frames Cell Events: 7875 mean_circ: 0.940693
Ran for 256050 frames Cell Events: 7886 mean_circ: 0.940694
Ran for 257550 frames Cell Events: 7934 mean_circ: 0.940665
Ran for 258050 frames Cell Events: 7953 mean_circ: 0.94068
Ran for 258550 frames Cell Events: 7964 mean_circ: 0.940709
Ran for 259050 frames Cell Events: 7979 mean_circ: 0.94072
Ran for 259550 frames Cell Events: 8005 mean_circ: 0.940738
Ran for 260550 frames Cell Events: 8036 mean_circ: 0.940699
Ran for 261050 frames Cell Events: 8052 mean_circ: 0.940705
Ran for 262050 frames Cell Events: 8086 mean_circ: 0.940635
Ran for 263050 frames Cell Events: 8129 mean_circ: 0.940689
Ran for 263550 frames Cell Events: 8144 mean_circ: 0.940684
Ran for 265050 frames Cell Events: 8190 mean_circ: 0.940653
Ran for 265550 frames Cell Events: 8200 mean_circ: 0.940664
Ran for 266550 frames Cell Events: 8226 mean_circ: 0.940661
Ran for 267050 frames Cell Events: 8233 mean_circ: 0.940654
Ran for 267550 frames Cell Events: 8255 mean_circ: 0.940672
End this measurment..
Stopping time: 2.12044e+009
****************************************
Measurement finished
Mean Circularitiy: 0.940681
Mean Area: 701.697
Mean FL-1 intensity: 3805.04
Mean FL-2 intensity: 4897.2
Mean FL-3 intensity: 0
frames processed: 266376
Total Time: 133861ms
Number of Runs (new bg img) 0
Frames collected: 267599
Skipped frames: 58018
Rescued Frames: 4264545
Samples DAQ: 66900909
Samples written: 133799302
Samples per Channel: 66899651
FPS: 1999.09
Samples per second: 499771
Expected samples/s: 500000
Events: 8257
Events per second: 61.6835
jumped back (PMT): 3862
#Frames read, frequency
# 0 frames :401165
# 1 frames :115376
# 2 frames :4175
# 3 frames :2104
# 4 frames :1283
# 5 frames :880
# 6 frames :709
# 7 frames :1384
# 8 frames :1192
# 9 frames :975
# 10 frames :939
# 11 frames :749
# 12 frames :583
# 13 frames :425
# 14 frames :358
# 15 frames :291
# 16 frames :294
# 17 frames :292
# 18 frames :312
# 19 frames :252
# 20 frames :208
# 21 frames :221
# 22 frames :142
# 23 frames :130
# 24 frames :79
# 25 frames :75
# 26 frames :64
# 27 frames :59
# 28 frames :49
# 29 frames :40
# 30 frames :40
# 31 frames :33
# 32 frames :21
# 33 frames :19
# 34 frames :26
# 35 frames :14
# 36 frames :19
# 37 frames :13
# 38 frames :14
# 39 frames :18
# 40 frames :13
# 41 frames :11
# 42 frames :13
# 43 frames :8
# 44 frames :4
# 45 frames :5
# 46 frames :3
# 47 frames :2
# 48 frames :2
# 49 frames :6
# 50 frames :6
# 51 frames :1
# 52 frames :1
# 53 frames :4
# 54 frames :1
# 55 frames :1
# 57 frames :2
# 60 frames :2
# 61 frames :1
# 62 frames :1
# 63 frames :1
# 71 frames :1
# 72 frames :1
# 74 frames :1
# 75 frames :1
# 87 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1528
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0