Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.49.20
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "4c347d4112c2b2c6c0ed818e4399bb71",
"name": "M1_20170608_2144_c2_s5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:49:44"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8951 (max 8952) in '8951'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2797
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.1217e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.952514
Ran for 1550 frames Cell Events: 53 mean_circ: 0.956847
Ran for 2050 frames Cell Events: 68 mean_circ: 0.957408
Ran for 4550 frames Cell Events: 124 mean_circ: 0.954883
Ran for 5050 frames Cell Events: 137 mean_circ: 0.953906
Ran for 8050 frames Cell Events: 226 mean_circ: 0.951336
Ran for 8550 frames Cell Events: 237 mean_circ: 0.950736
Ran for 10550 frames Cell Events: 305 mean_circ: 0.950384
Ran for 11050 frames Cell Events: 318 mean_circ: 0.950617
Ran for 11550 frames Cell Events: 333 mean_circ: 0.951289
Ran for 12050 frames Cell Events: 347 mean_circ: 0.950907
Ran for 12550 frames Cell Events: 362 mean_circ: 0.950478
Ran for 13050 frames Cell Events: 377 mean_circ: 0.949877
Ran for 13550 frames Cell Events: 387 mean_circ: 0.949423
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Ran for 23050 frames Cell Events: 655 mean_circ: 0.950486
Ran for 23550 frames Cell Events: 668 mean_circ: 0.950484
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Ran for 84050 frames Cell Events: 2469 mean_circ: 0.947656
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Ran for 86550 frames Cell Events: 2535 mean_circ: 0.947735
Ran for 87050 frames Cell Events: 2542 mean_circ: 0.947788
Ran for 87550 frames Cell Events: 2557 mean_circ: 0.947837
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Ran for 89550 frames Cell Events: 2631 mean_circ: 0.947713
Ran for 92050 frames Cell Events: 2706 mean_circ: 0.94774
Ran for 92550 frames Cell Events: 2724 mean_circ: 0.947748
Ran for 93050 frames Cell Events: 2737 mean_circ: 0.94766
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Ran for 94050 frames Cell Events: 2760 mean_circ: 0.94768
Ran for 95050 frames Cell Events: 2793 mean_circ: 0.947792
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Ran for 96050 frames Cell Events: 2811 mean_circ: 0.947781
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Ran for 97050 frames Cell Events: 2835 mean_circ: 0.947824
Ran for 97550 frames Cell Events: 2853 mean_circ: 0.947822
Ran for 99550 frames Cell Events: 2902 mean_circ: 0.947737
Ran for 100550 frames Cell Events: 2940 mean_circ: 0.947757
Ran for 102550 frames Cell Events: 3011 mean_circ: 0.947699
Ran for 103050 frames Cell Events: 3028 mean_circ: 0.947803
Ran for 104050 frames Cell Events: 3054 mean_circ: 0.947579
Ran for 104550 frames Cell Events: 3064 mean_circ: 0.947578
Ran for 105050 frames Cell Events: 3076 mean_circ: 0.947503
Ran for 106050 frames Cell Events: 3099 mean_circ: 0.947504
Ran for 106550 frames Cell Events: 3114 mean_circ: 0.947452
Ran for 107550 frames Cell Events: 3143 mean_circ: 0.947427
Ran for 108050 frames Cell Events: 3157 mean_circ: 0.947381
Ran for 108550 frames Cell Events: 3165 mean_circ: 0.947376
Ran for 109050 frames Cell Events: 3179 mean_circ: 0.947429
Ran for 110050 frames Cell Events: 3199 mean_circ: 0.947418
Ran for 110550 frames Cell Events: 3216 mean_circ: 0.947438
Ran for 111050 frames Cell Events: 3228 mean_circ: 0.947418
Ran for 112050 frames Cell Events: 3249 mean_circ: 0.947421
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Ran for 113550 frames Cell Events: 3299 mean_circ: 0.947387
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Ran for 120050 frames Cell Events: 3469 mean_circ: 0.947372
Ran for 121050 frames Cell Events: 3495 mean_circ: 0.94742
Ran for 121550 frames Cell Events: 3507 mean_circ: 0.947452
Ran for 123050 frames Cell Events: 3563 mean_circ: 0.9474
Ran for 123550 frames Cell Events: 3580 mean_circ: 0.947394
Ran for 124050 frames Cell Events: 3590 mean_circ: 0.947412
Ran for 124550 frames Cell Events: 3596 mean_circ: 0.947361
Ran for 125550 frames Cell Events: 3611 mean_circ: 0.947358
Ran for 126050 frames Cell Events: 3621 mean_circ: 0.94736
Ran for 126550 frames Cell Events: 3635 mean_circ: 0.94739
Ran for 127050 frames Cell Events: 3650 mean_circ: 0.947337
Ran for 127550 frames Cell Events: 3662 mean_circ: 0.947353
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Ran for 131550 frames Cell Events: 3753 mean_circ: 0.947282
Ran for 132050 frames Cell Events: 3768 mean_circ: 0.947214
Ran for 132550 frames Cell Events: 3779 mean_circ: 0.947205
Ran for 133050 frames Cell Events: 3787 mean_circ: 0.947216
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Ran for 134050 frames Cell Events: 3816 mean_circ: 0.947134
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Ran for 142050 frames Cell Events: 4051 mean_circ: 0.947158
Ran for 142550 frames Cell Events: 4060 mean_circ: 0.947203
Ran for 143050 frames Cell Events: 4074 mean_circ: 0.947226
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Ran for 148050 frames Cell Events: 4234 mean_circ: 0.947048
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Ran for 151050 frames Cell Events: 4322 mean_circ: 0.947074
Ran for 151550 frames Cell Events: 4336 mean_circ: 0.947038
Ran for 152050 frames Cell Events: 4362 mean_circ: 0.947067
Ran for 153050 frames Cell Events: 4420 mean_circ: 0.947023
Ran for 154050 frames Cell Events: 4457 mean_circ: 0.947014
Ran for 156050 frames Cell Events: 4521 mean_circ: 0.946884
Ran for 156550 frames Cell Events: 4539 mean_circ: 0.946881
Ran for 159050 frames Cell Events: 4616 mean_circ: 0.946843
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Ran for 161050 frames Cell Events: 4680 mean_circ: 0.946838
Ran for 161550 frames Cell Events: 4693 mean_circ: 0.946858
Ran for 162550 frames Cell Events: 4721 mean_circ: 0.946889
Ran for 163050 frames Cell Events: 4739 mean_circ: 0.946897
Ran for 163550 frames Cell Events: 4749 mean_circ: 0.946926
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Ran for 166550 frames Cell Events: 4843 mean_circ: 0.946987
Ran for 167050 frames Cell Events: 4860 mean_circ: 0.946997
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Ran for 208050 frames Cell Events: 6167 mean_circ: 0.946783
Ran for 209050 frames Cell Events: 6201 mean_circ: 0.946808
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Ran for 223550 frames Cell Events: 6642 mean_circ: 0.946692
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Ran for 225550 frames Cell Events: 6720 mean_circ: 0.946728
Ran for 226050 frames Cell Events: 6739 mean_circ: 0.946729
Ran for 227050 frames Cell Events: 6771 mean_circ: 0.946697
Ran for 228050 frames Cell Events: 6800 mean_circ: 0.946755
Ran for 230050 frames Cell Events: 6849 mean_circ: 0.946725
Ran for 231050 frames Cell Events: 6885 mean_circ: 0.946709
Ran for 231550 frames Cell Events: 6900 mean_circ: 0.946715
Ran for 232550 frames Cell Events: 6933 mean_circ: 0.946712
Ran for 233550 frames Cell Events: 6957 mean_circ: 0.946745
Ran for 234050 frames Cell Events: 6970 mean_circ: 0.946754
Ran for 234550 frames Cell Events: 6991 mean_circ: 0.946768
Ran for 237050 frames Cell Events: 7074 mean_circ: 0.946827
Ran for 237550 frames Cell Events: 7089 mean_circ: 0.946839
Ran for 238050 frames Cell Events: 7112 mean_circ: 0.946784
Ran for 238550 frames Cell Events: 7121 mean_circ: 0.946788
Ran for 239050 frames Cell Events: 7136 mean_circ: 0.946802
Ran for 240550 frames Cell Events: 7182 mean_circ: 0.946841
Ran for 241050 frames Cell Events: 7190 mean_circ: 0.946838
Ran for 241550 frames Cell Events: 7197 mean_circ: 0.946817
Ran for 242550 frames Cell Events: 7233 mean_circ: 0.946796
Ran for 243050 frames Cell Events: 7247 mean_circ: 0.946822
Ran for 244550 frames Cell Events: 7291 mean_circ: 0.946849
Ran for 245050 frames Cell Events: 7302 mean_circ: 0.946798
Ran for 245550 frames Cell Events: 7312 mean_circ: 0.946814
Ran for 246050 frames Cell Events: 7323 mean_circ: 0.946806
Ran for 247050 frames Cell Events: 7357 mean_circ: 0.946786
Ran for 247550 frames Cell Events: 7366 mean_circ: 0.946789
Ran for 248050 frames Cell Events: 7379 mean_circ: 0.946761
Ran for 249050 frames Cell Events: 7420 mean_circ: 0.946751
Ran for 250050 frames Cell Events: 7455 mean_circ: 0.94674
Ran for 250550 frames Cell Events: 7469 mean_circ: 0.946666
Ran for 251050 frames Cell Events: 7492 mean_circ: 0.946674
Ran for 252550 frames Cell Events: 7535 mean_circ: 0.946653
Ran for 253050 frames Cell Events: 7551 mean_circ: 0.946665
Ran for 254550 frames Cell Events: 7602 mean_circ: 0.946598
Ran for 255050 frames Cell Events: 7615 mean_circ: 0.946611
Ran for 255550 frames Cell Events: 7628 mean_circ: 0.946625
Ran for 256050 frames Cell Events: 7641 mean_circ: 0.946653
Ran for 257050 frames Cell Events: 7669 mean_circ: 0.946627
Ran for 257550 frames Cell Events: 7689 mean_circ: 0.946629
Ran for 259550 frames Cell Events: 7745 mean_circ: 0.946698
Ran for 260050 frames Cell Events: 7755 mean_circ: 0.946665
Ran for 260550 frames Cell Events: 7769 mean_circ: 0.946657
Ran for 261050 frames Cell Events: 7780 mean_circ: 0.946687
Ran for 262050 frames Cell Events: 7811 mean_circ: 0.946684
Ran for 263050 frames Cell Events: 7835 mean_circ: 0.94663
Ran for 263550 frames Cell Events: 7848 mean_circ: 0.946628
Ran for 264050 frames Cell Events: 7861 mean_circ: 0.946645
Ran for 265550 frames Cell Events: 7925 mean_circ: 0.946627
Ran for 266050 frames Cell Events: 7938 mean_circ: 0.946624
Ran for 267050 frames Cell Events: 7976 mean_circ: 0.946622
Ran for 267550 frames Cell Events: 7992 mean_circ: 0.946631
Ran for 268050 frames Cell Events: 8004 mean_circ: 0.946627
Ran for 271050 frames Cell Events: 8111 mean_circ: 0.946555
Ran for 272050 frames Cell Events: 8154 mean_circ: 0.946566
Ran for 272550 frames Cell Events: 8165 mean_circ: 0.946536
Ran for 273550 frames Cell Events: 8198 mean_circ: 0.94656
Ran for 274050 frames Cell Events: 8211 mean_circ: 0.946528
Ran for 275050 frames Cell Events: 8236 mean_circ: 0.946561
Ran for 275550 frames Cell Events: 8253 mean_circ: 0.946563
Ran for 277550 frames Cell Events: 8319 mean_circ: 0.946535
Ran for 278050 frames Cell Events: 8328 mean_circ: 0.946533
Ran for 278550 frames Cell Events: 8341 mean_circ: 0.946519
Ran for 279050 frames Cell Events: 8346 mean_circ: 0.946517
Ran for 280550 frames Cell Events: 8392 mean_circ: 0.946484
Ran for 281050 frames Cell Events: 8411 mean_circ: 0.946484
Ran for 284550 frames Cell Events: 8518 mean_circ: 0.946409
Ran for 285050 frames Cell Events: 8524 mean_circ: 0.94641
Ran for 285550 frames Cell Events: 8540 mean_circ: 0.946402
Ran for 286050 frames Cell Events: 8558 mean_circ: 0.946366
Ran for 287550 frames Cell Events: 8605 mean_circ: 0.946396
Ran for 288050 frames Cell Events: 8619 mean_circ: 0.946403
Ran for 289050 frames Cell Events: 8642 mean_circ: 0.946405
Ran for 289550 frames Cell Events: 8656 mean_circ: 0.946362
Ran for 290050 frames Cell Events: 8666 mean_circ: 0.94638
Ran for 290550 frames Cell Events: 8686 mean_circ: 0.946352
Ran for 292550 frames Cell Events: 8748 mean_circ: 0.946356
Ran for 293050 frames Cell Events: 8759 mean_circ: 0.946371
Ran for 293550 frames Cell Events: 8770 mean_circ: 0.946374
Ran for 294050 frames Cell Events: 8784 mean_circ: 0.946373
Ran for 295050 frames Cell Events: 8810 mean_circ: 0.946313
Ran for 295550 frames Cell Events: 8824 mean_circ: 0.9463
Ran for 296050 frames Cell Events: 8835 mean_circ: 0.946287
Ran for 297050 frames Cell Events: 8858 mean_circ: 0.946324
Ran for 297550 frames Cell Events: 8880 mean_circ: 0.94634
Ran for 298550 frames Cell Events: 8910 mean_circ: 0.946342
Ran for 299050 frames Cell Events: 8924 mean_circ: 0.946346
Ran for 299550 frames Cell Events: 8942 mean_circ: 0.946355
Ran for 300050 frames Cell Events: 8950 mean_circ: 0.946379
End this measurment..
Stopping time: 2.12185e+009
****************************************
Measurement finished
Mean Circularitiy: 0.946377
Mean Area: 666.846
Mean FL-1 intensity: 3914.2
Mean FL-2 intensity: 5131.34
Mean FL-3 intensity: 0
frames processed: 298143
Total Time: 150132ms
Number of Runs (new bg img) 0
Frames collected: 300144
Skipped frames: 67147
Rescued Frames: 4748084
Samples DAQ: 75038204
Samples written: 150071860
Samples per Channel: 75035930
FPS: 1999.2
Samples per second: 499800
Expected samples/s: 500000
Events: 8952
Events per second: 59.6275
jumped back (PMT): 3994
#Frames read, frequency
# 0 frames :451788
# 1 frames :128144
# 2 frames :4244
# 3 frames :2465
# 4 frames :1431
# 5 frames :992
# 6 frames :737
# 7 frames :1430
# 8 frames :1366
# 9 frames :1030
# 10 frames :884
# 11 frames :778
# 12 frames :667
# 13 frames :586
# 14 frames :408
# 15 frames :335
# 16 frames :294
# 17 frames :314
# 18 frames :325
# 19 frames :331
# 20 frames :286
# 21 frames :249
# 22 frames :204
# 23 frames :161
# 24 frames :116
# 25 frames :86
# 26 frames :76
# 27 frames :59
# 28 frames :56
# 29 frames :61
# 30 frames :36
# 31 frames :33
# 32 frames :30
# 33 frames :24
# 34 frames :28
# 35 frames :23
# 36 frames :24
# 37 frames :12
# 38 frames :16
# 39 frames :20
# 40 frames :12
# 41 frames :9
# 42 frames :7
# 43 frames :12
# 44 frames :6
# 45 frames :6
# 46 frames :8
# 47 frames :5
# 48 frames :6
# 49 frames :8
# 50 frames :2
# 51 frames :3
# 52 frames :4
# 53 frames :5
# 54 frames :5
# 55 frames :2
# 56 frames :1
# 57 frames :4
# 58 frames :1
# 59 frames :1
# 60 frames :2
# 61 frames :2
# 62 frames :1
# 63 frames :1
# 70 frames :1
# 74 frames :1
# 83 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2797
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0