Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.52.04
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "08e720a5e55111e89420c20cfa848222",
"name": "M1_20170608_2212_c2_s7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:52:28"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9160 (max 9161) in '9160'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2494
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.12333e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 44 mean_circ: 0.929017
Ran for 2050 frames Cell Events: 77 mean_circ: 0.933497
Ran for 2550 frames Cell Events: 91 mean_circ: 0.931197
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Ran for 86550 frames Cell Events: 2907 mean_circ: 0.930781
Ran for 87050 frames Cell Events: 2921 mean_circ: 0.930771
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Ran for 91050 frames Cell Events: 3051 mean_circ: 0.93079
Ran for 92550 frames Cell Events: 3099 mean_circ: 0.930851
Ran for 93550 frames Cell Events: 3130 mean_circ: 0.930783
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Ran for 96550 frames Cell Events: 3244 mean_circ: 0.930939
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Ran for 97550 frames Cell Events: 3282 mean_circ: 0.931062
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Ran for 122550 frames Cell Events: 4178 mean_circ: 0.931223
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Ran for 123550 frames Cell Events: 4202 mean_circ: 0.931304
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Ran for 126050 frames Cell Events: 4287 mean_circ: 0.931445
Ran for 126550 frames Cell Events: 4297 mean_circ: 0.931382
Ran for 127050 frames Cell Events: 4312 mean_circ: 0.931333
Ran for 127550 frames Cell Events: 4333 mean_circ: 0.931378
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Ran for 131550 frames Cell Events: 4529 mean_circ: 0.931258
Ran for 132050 frames Cell Events: 4554 mean_circ: 0.931248
Ran for 132550 frames Cell Events: 4583 mean_circ: 0.931271
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Ran for 192550 frames Cell Events: 6756 mean_circ: 0.931021
Ran for 193050 frames Cell Events: 6759 mean_circ: 0.931034
Ran for 193550 frames Cell Events: 6777 mean_circ: 0.931083
Ran for 194050 frames Cell Events: 6803 mean_circ: 0.931081
Ran for 195050 frames Cell Events: 6853 mean_circ: 0.931076
Ran for 195550 frames Cell Events: 6865 mean_circ: 0.931083
Ran for 196050 frames Cell Events: 6892 mean_circ: 0.931091
Ran for 198050 frames Cell Events: 6971 mean_circ: 0.931097
Ran for 198550 frames Cell Events: 6994 mean_circ: 0.931093
Ran for 199050 frames Cell Events: 7007 mean_circ: 0.931049
Ran for 200050 frames Cell Events: 7047 mean_circ: 0.931102
Ran for 200550 frames Cell Events: 7072 mean_circ: 0.931153
Ran for 201550 frames Cell Events: 7116 mean_circ: 0.931079
Ran for 202050 frames Cell Events: 7143 mean_circ: 0.931083
Ran for 203050 frames Cell Events: 7192 mean_circ: 0.931092
Ran for 204050 frames Cell Events: 7233 mean_circ: 0.931101
Ran for 205550 frames Cell Events: 7313 mean_circ: 0.931146
Ran for 206050 frames Cell Events: 7324 mean_circ: 0.931112
Ran for 206550 frames Cell Events: 7335 mean_circ: 0.931113
Ran for 207050 frames Cell Events: 7361 mean_circ: 0.931096
Ran for 207550 frames Cell Events: 7380 mean_circ: 0.931145
Ran for 208050 frames Cell Events: 7398 mean_circ: 0.931139
Ran for 208550 frames Cell Events: 7419 mean_circ: 0.931171
Ran for 213550 frames Cell Events: 7684 mean_circ: 0.930959
Ran for 214050 frames Cell Events: 7694 mean_circ: 0.930962
Ran for 214550 frames Cell Events: 7709 mean_circ: 0.93101
Ran for 215050 frames Cell Events: 7714 mean_circ: 0.93103
Ran for 215550 frames Cell Events: 7723 mean_circ: 0.931041
Ran for 216050 frames Cell Events: 7742 mean_circ: 0.931015
Ran for 217050 frames Cell Events: 7790 mean_circ: 0.93099
Ran for 217550 frames Cell Events: 7814 mean_circ: 0.930988
Ran for 218050 frames Cell Events: 7829 mean_circ: 0.930987
Ran for 219050 frames Cell Events: 7860 mean_circ: 0.930931
Ran for 219550 frames Cell Events: 7878 mean_circ: 0.930912
Ran for 220050 frames Cell Events: 7899 mean_circ: 0.930942
Ran for 220550 frames Cell Events: 7912 mean_circ: 0.93096
Ran for 221050 frames Cell Events: 7927 mean_circ: 0.930952
Ran for 222550 frames Cell Events: 7978 mean_circ: 0.93098
Ran for 224050 frames Cell Events: 8051 mean_circ: 0.930965
Ran for 224550 frames Cell Events: 8070 mean_circ: 0.930958
Ran for 225050 frames Cell Events: 8088 mean_circ: 0.930959
Ran for 225550 frames Cell Events: 8108 mean_circ: 0.930964
Ran for 227050 frames Cell Events: 8157 mean_circ: 0.930907
Ran for 227550 frames Cell Events: 8174 mean_circ: 0.930904
Ran for 228050 frames Cell Events: 8196 mean_circ: 0.930909
Ran for 228550 frames Cell Events: 8217 mean_circ: 0.930912
Ran for 229550 frames Cell Events: 8264 mean_circ: 0.930867
Ran for 230050 frames Cell Events: 8280 mean_circ: 0.930859
Ran for 230550 frames Cell Events: 8289 mean_circ: 0.930833
Ran for 231550 frames Cell Events: 8327 mean_circ: 0.930852
Ran for 232050 frames Cell Events: 8348 mean_circ: 0.930863
Ran for 232550 frames Cell Events: 8365 mean_circ: 0.930878
Ran for 233050 frames Cell Events: 8374 mean_circ: 0.930877
Ran for 233550 frames Cell Events: 8388 mean_circ: 0.930827
Ran for 234050 frames Cell Events: 8394 mean_circ: 0.930841
Ran for 235050 frames Cell Events: 8429 mean_circ: 0.930821
Ran for 235550 frames Cell Events: 8445 mean_circ: 0.93082
Ran for 236050 frames Cell Events: 8472 mean_circ: 0.930842
Ran for 236550 frames Cell Events: 8497 mean_circ: 0.930831
Ran for 237050 frames Cell Events: 8508 mean_circ: 0.930838
Ran for 238050 frames Cell Events: 8557 mean_circ: 0.93082
Ran for 238550 frames Cell Events: 8574 mean_circ: 0.930808
Ran for 239550 frames Cell Events: 8620 mean_circ: 0.930757
Ran for 240050 frames Cell Events: 8642 mean_circ: 0.930758
Ran for 240550 frames Cell Events: 8662 mean_circ: 0.930784
Ran for 241050 frames Cell Events: 8671 mean_circ: 0.930789
Ran for 242550 frames Cell Events: 8748 mean_circ: 0.930789
Ran for 243050 frames Cell Events: 8760 mean_circ: 0.930804
Ran for 243550 frames Cell Events: 8775 mean_circ: 0.930807
Ran for 244050 frames Cell Events: 8792 mean_circ: 0.930806
Ran for 244550 frames Cell Events: 8803 mean_circ: 0.930788
Ran for 245550 frames Cell Events: 8832 mean_circ: 0.93081
Ran for 246050 frames Cell Events: 8852 mean_circ: 0.930797
Ran for 247050 frames Cell Events: 8893 mean_circ: 0.930838
Ran for 248050 frames Cell Events: 8928 mean_circ: 0.930822
Ran for 248550 frames Cell Events: 8942 mean_circ: 0.930765
Ran for 249550 frames Cell Events: 8987 mean_circ: 0.930763
Ran for 250050 frames Cell Events: 8998 mean_circ: 0.930748
Ran for 250550 frames Cell Events: 9018 mean_circ: 0.930745
Ran for 251050 frames Cell Events: 9033 mean_circ: 0.930763
Ran for 251550 frames Cell Events: 9054 mean_circ: 0.930769
Ran for 253550 frames Cell Events: 9123 mean_circ: 0.930746
Ran for 254050 frames Cell Events: 9133 mean_circ: 0.930753
Ran for 254550 frames Cell Events: 9161 mean_circ: 0.930727
End this measurment..
Stopping time: 2.12346e+009
****************************************
Measurement finished
Mean Circularitiy: 0.930748
Mean Area: 663.379
Mean FL-1 intensity: 3899.1
Mean FL-2 intensity: 5220.61
Mean FL-3 intensity: 0
frames processed: 254548
Total Time: 127361ms
Number of Runs (new bg img) 0
Frames collected: 254616
Skipped frames: 73719
Rescued Frames: 5257027
Samples DAQ: 63654080
Samples written: 127308110
Samples per Channel: 63654055
FPS: 1999.17
Samples per second: 499792
Expected samples/s: 500000
Events: 9161
Events per second: 71.9293
jumped back (PMT): 10
#Frames read, frequency
# 0 frames :356429
# 1 frames :133527
# 2 frames :5417
# 3 frames :2159
# 4 frames :1042
# 5 frames :447
# 6 frames :1115
# 7 frames :2959
# 8 frames :1051
# 9 frames :784
# 10 frames :1618
# 11 frames :769
# 12 frames :475
# 13 frames :286
# 14 frames :153
# 15 frames :204
# 16 frames :173
# 17 frames :161
# 18 frames :92
# 19 frames :52
# 20 frames :56
# 21 frames :58
# 22 frames :35
# 23 frames :31
# 24 frames :28
# 25 frames :20
# 26 frames :12
# 27 frames :7
# 28 frames :11
# 29 frames :4
# 30 frames :6
# 31 frames :7
# 32 frames :3
# 33 frames :2
# 34 frames :2
# 35 frames :1
# 36 frames :1
# 37 frames :1
# 38 frames :2
# 39 frames :1
# 40 frames :1
# 41 frames :1
# 43 frames :1
# 45 frames :1
# 46 frames :1
# 49 frames :1
# 68 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2494
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0