Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_14.56.19
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c673d30b841e52f7c307f0f57ae954b1",
"name": "M1_20170609_1242_dsRNA_X3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:56:40"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8094 (max 8095) in '8094'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0154
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.17556e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.949366
Ran for 1050 frames Cell Events: 33 mean_circ: 0.940483
Ran for 1550 frames Cell Events: 56 mean_circ: 0.938506
Ran for 2050 frames Cell Events: 71 mean_circ: 0.936587
Ran for 2550 frames Cell Events: 80 mean_circ: 0.934593
Ran for 3050 frames Cell Events: 96 mean_circ: 0.934858
Ran for 4050 frames Cell Events: 120 mean_circ: 0.937466
Ran for 4550 frames Cell Events: 137 mean_circ: 0.936199
Ran for 6050 frames Cell Events: 183 mean_circ: 0.939812
Ran for 7050 frames Cell Events: 217 mean_circ: 0.941159
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Ran for 12550 frames Cell Events: 396 mean_circ: 0.942027
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Ran for 15050 frames Cell Events: 475 mean_circ: 0.942887
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Ran for 64550 frames Cell Events: 1957 mean_circ: 0.942123
Ran for 65050 frames Cell Events: 1974 mean_circ: 0.942206
Ran for 65550 frames Cell Events: 1996 mean_circ: 0.94219
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Ran for 84050 frames Cell Events: 2630 mean_circ: 0.942202
Ran for 84550 frames Cell Events: 2653 mean_circ: 0.942183
Ran for 85050 frames Cell Events: 2662 mean_circ: 0.942249
Ran for 85550 frames Cell Events: 2679 mean_circ: 0.942164
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Ran for 87050 frames Cell Events: 2717 mean_circ: 0.941981
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Ran for 91550 frames Cell Events: 2863 mean_circ: 0.941493
Ran for 92050 frames Cell Events: 2876 mean_circ: 0.941458
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Ran for 107050 frames Cell Events: 3372 mean_circ: 0.940982
Ran for 107550 frames Cell Events: 3397 mean_circ: 0.940825
Ran for 108550 frames Cell Events: 3422 mean_circ: 0.940765
Ran for 109050 frames Cell Events: 3436 mean_circ: 0.940781
Ran for 110550 frames Cell Events: 3496 mean_circ: 0.940643
Ran for 111050 frames Cell Events: 3515 mean_circ: 0.940716
Ran for 112050 frames Cell Events: 3549 mean_circ: 0.940584
Ran for 112550 frames Cell Events: 3559 mean_circ: 0.94064
Ran for 113050 frames Cell Events: 3573 mean_circ: 0.940625
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Ran for 121050 frames Cell Events: 3877 mean_circ: 0.940408
Ran for 122050 frames Cell Events: 3907 mean_circ: 0.940373
Ran for 123050 frames Cell Events: 3952 mean_circ: 0.940418
Ran for 123550 frames Cell Events: 3960 mean_circ: 0.940343
Ran for 124050 frames Cell Events: 3972 mean_circ: 0.940372
Ran for 124550 frames Cell Events: 3986 mean_circ: 0.940338
Ran for 126050 frames Cell Events: 4034 mean_circ: 0.940336
Ran for 127050 frames Cell Events: 4068 mean_circ: 0.940243
Ran for 127550 frames Cell Events: 4084 mean_circ: 0.940292
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Ran for 131050 frames Cell Events: 4200 mean_circ: 0.940182
Ran for 131550 frames Cell Events: 4224 mean_circ: 0.940236
Ran for 132550 frames Cell Events: 4252 mean_circ: 0.940217
Ran for 133050 frames Cell Events: 4267 mean_circ: 0.940257
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Ran for 142050 frames Cell Events: 4552 mean_circ: 0.940383
Ran for 143550 frames Cell Events: 4600 mean_circ: 0.94039
Ran for 144050 frames Cell Events: 4614 mean_circ: 0.940429
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Ran for 188050 frames Cell Events: 6115 mean_circ: 0.939595
Ran for 188550 frames Cell Events: 6138 mean_circ: 0.939576
Ran for 189050 frames Cell Events: 6157 mean_circ: 0.939569
Ran for 190050 frames Cell Events: 6190 mean_circ: 0.939616
Ran for 190550 frames Cell Events: 6209 mean_circ: 0.93964
Ran for 191050 frames Cell Events: 6225 mean_circ: 0.939637
Ran for 191550 frames Cell Events: 6243 mean_circ: 0.939679
Ran for 192050 frames Cell Events: 6258 mean_circ: 0.939657
Ran for 192550 frames Cell Events: 6277 mean_circ: 0.939709
Ran for 193050 frames Cell Events: 6291 mean_circ: 0.939752
Ran for 193550 frames Cell Events: 6311 mean_circ: 0.93972
Ran for 194550 frames Cell Events: 6355 mean_circ: 0.939738
Ran for 195050 frames Cell Events: 6378 mean_circ: 0.939761
Ran for 195550 frames Cell Events: 6392 mean_circ: 0.939753
Ran for 196050 frames Cell Events: 6416 mean_circ: 0.939696
Ran for 198050 frames Cell Events: 6493 mean_circ: 0.9397
Ran for 198550 frames Cell Events: 6513 mean_circ: 0.939716
Ran for 199550 frames Cell Events: 6551 mean_circ: 0.939682
Ran for 200050 frames Cell Events: 6563 mean_circ: 0.939652
Ran for 200550 frames Cell Events: 6576 mean_circ: 0.939606
Ran for 201050 frames Cell Events: 6604 mean_circ: 0.939551
Ran for 202050 frames Cell Events: 6632 mean_circ: 0.939508
Ran for 202550 frames Cell Events: 6644 mean_circ: 0.93949
Ran for 203550 frames Cell Events: 6681 mean_circ: 0.939502
Ran for 204050 frames Cell Events: 6699 mean_circ: 0.939462
Ran for 204550 frames Cell Events: 6714 mean_circ: 0.939468
Ran for 205550 frames Cell Events: 6742 mean_circ: 0.939465
Ran for 206050 frames Cell Events: 6754 mean_circ: 0.93947
Ran for 206550 frames Cell Events: 6778 mean_circ: 0.939424
Ran for 207050 frames Cell Events: 6795 mean_circ: 0.939458
Ran for 207550 frames Cell Events: 6813 mean_circ: 0.939434
Ran for 208050 frames Cell Events: 6827 mean_circ: 0.939437
Ran for 209050 frames Cell Events: 6868 mean_circ: 0.939419
Ran for 209550 frames Cell Events: 6883 mean_circ: 0.939418
Ran for 210050 frames Cell Events: 6906 mean_circ: 0.939433
Ran for 211050 frames Cell Events: 6957 mean_circ: 0.939425
Ran for 211550 frames Cell Events: 6972 mean_circ: 0.939391
Ran for 212050 frames Cell Events: 6981 mean_circ: 0.939384
Ran for 212550 frames Cell Events: 7000 mean_circ: 0.939409
Ran for 213050 frames Cell Events: 7009 mean_circ: 0.939417
Ran for 213550 frames Cell Events: 7022 mean_circ: 0.939399
Ran for 214050 frames Cell Events: 7040 mean_circ: 0.939403
Ran for 214550 frames Cell Events: 7051 mean_circ: 0.939424
Ran for 215050 frames Cell Events: 7082 mean_circ: 0.939418
Ran for 215550 frames Cell Events: 7095 mean_circ: 0.939456
Ran for 216050 frames Cell Events: 7112 mean_circ: 0.93946
Ran for 216550 frames Cell Events: 7121 mean_circ: 0.939495
Ran for 217050 frames Cell Events: 7136 mean_circ: 0.939458
Ran for 218050 frames Cell Events: 7157 mean_circ: 0.939421
Ran for 218550 frames Cell Events: 7184 mean_circ: 0.93934
Ran for 219050 frames Cell Events: 7193 mean_circ: 0.939362
Ran for 219550 frames Cell Events: 7211 mean_circ: 0.939342
Ran for 222550 frames Cell Events: 7291 mean_circ: 0.939137
Ran for 224050 frames Cell Events: 7350 mean_circ: 0.939144
Ran for 224550 frames Cell Events: 7362 mean_circ: 0.939142
Ran for 225550 frames Cell Events: 7394 mean_circ: 0.93912
Ran for 226050 frames Cell Events: 7414 mean_circ: 0.939075
Ran for 226550 frames Cell Events: 7429 mean_circ: 0.939074
Ran for 227050 frames Cell Events: 7446 mean_circ: 0.939079
Ran for 228550 frames Cell Events: 7512 mean_circ: 0.939092
Ran for 229050 frames Cell Events: 7527 mean_circ: 0.939108
Ran for 230050 frames Cell Events: 7568 mean_circ: 0.939118
Ran for 230550 frames Cell Events: 7584 mean_circ: 0.939115
Ran for 231050 frames Cell Events: 7598 mean_circ: 0.939118
Ran for 231550 frames Cell Events: 7611 mean_circ: 0.939136
Ran for 232050 frames Cell Events: 7628 mean_circ: 0.939139
Ran for 232550 frames Cell Events: 7645 mean_circ: 0.939162
Ran for 233050 frames Cell Events: 7661 mean_circ: 0.939159
Ran for 233550 frames Cell Events: 7679 mean_circ: 0.939168
Ran for 234050 frames Cell Events: 7690 mean_circ: 0.939173
Ran for 235050 frames Cell Events: 7729 mean_circ: 0.939199
Ran for 236550 frames Cell Events: 7772 mean_circ: 0.939119
Ran for 237050 frames Cell Events: 7786 mean_circ: 0.93909
Ran for 237550 frames Cell Events: 7800 mean_circ: 0.939097
Ran for 238550 frames Cell Events: 7839 mean_circ: 0.93907
Ran for 239550 frames Cell Events: 7879 mean_circ: 0.939063
Ran for 240050 frames Cell Events: 7893 mean_circ: 0.939058
Ran for 240550 frames Cell Events: 7914 mean_circ: 0.93901
Ran for 241050 frames Cell Events: 7929 mean_circ: 0.939022
Ran for 242050 frames Cell Events: 7965 mean_circ: 0.93899
Ran for 242550 frames Cell Events: 7979 mean_circ: 0.938948
Ran for 243050 frames Cell Events: 7995 mean_circ: 0.938941
Ran for 244550 frames Cell Events: 8046 mean_circ: 0.938936
Ran for 245050 frames Cell Events: 8060 mean_circ: 0.938949
Ran for 245550 frames Cell Events: 8077 mean_circ: 0.938957
End this measurment..
Stopping time: 2.17568e+009
****************************************
Measurement finished
Mean Circularitiy: 0.938988
Mean Area: 597.199
Mean FL-1 intensity: 3128.19
Mean FL-2 intensity: 4243.81
Mean FL-3 intensity: 0
frames processed: 244259
Total Time: 123031ms
Number of Runs (new bg img) 0
Frames collected: 245910
Skipped frames: 10047
Rescued Frames: 929205
Samples DAQ: 61480315
Samples written: 122955050
Samples per Channel: 61477525
FPS: 1998.76
Samples per second: 499690
Expected samples/s: 500000
Events: 8095
Events per second: 65.7963
jumped back (PMT): 18
#Frames read, frequency
# 0 frames :339321
# 1 frames :134650
# 2 frames :4917
# 3 frames :1928
# 4 frames :1082
# 5 frames :424
# 6 frames :1981
# 7 frames :1821
# 8 frames :1349
# 9 frames :795
# 10 frames :921
# 11 frames :837
# 12 frames :624
# 13 frames :250
# 14 frames :155
# 15 frames :147
# 16 frames :117
# 17 frames :111
# 18 frames :57
# 19 frames :61
# 20 frames :46
# 21 frames :40
# 22 frames :29
# 23 frames :14
# 24 frames :17
# 25 frames :17
# 26 frames :10
# 27 frames :7
# 28 frames :11
# 29 frames :4
# 30 frames :3
# 31 frames :6
# 32 frames :5
# 33 frames :5
# 34 frames :3
# 35 frames :1
# 36 frames :3
# 39 frames :1
# 41 frames :1
# 43 frames :1
# 45 frames :1
# 49 frames :1
# 56 frames :2
# 74 frames :1
# 78 frames :1
# 79 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0154
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0