Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_15.02.11
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "29f3c2fef6689f6f5e73660c1078bae0",
"name": "M1_20170609_1358_dsRNA_Y1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "13:02:31"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8108 (max 8109) in '8108'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2508
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.1801e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 29 mean_circ: 0.950144
Ran for 1550 frames Cell Events: 50 mean_circ: 0.945134
Ran for 2050 frames Cell Events: 63 mean_circ: 0.940377
Ran for 2550 frames Cell Events: 87 mean_circ: 0.939133
Ran for 3050 frames Cell Events: 99 mean_circ: 0.942573
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Ran for 5550 frames Cell Events: 166 mean_circ: 0.940721
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Ran for 73050 frames Cell Events: 2350 mean_circ: 0.94164
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Ran for 83050 frames Cell Events: 2635 mean_circ: 0.940901
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Ran for 84550 frames Cell Events: 2682 mean_circ: 0.940924
Ran for 86050 frames Cell Events: 2720 mean_circ: 0.940768
Ran for 86550 frames Cell Events: 2741 mean_circ: 0.940831
Ran for 88550 frames Cell Events: 2801 mean_circ: 0.940789
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Ran for 90550 frames Cell Events: 2860 mean_circ: 0.940843
Ran for 91050 frames Cell Events: 2875 mean_circ: 0.94089
Ran for 93050 frames Cell Events: 2998 mean_circ: 0.941112
Ran for 93550 frames Cell Events: 3027 mean_circ: 0.941086
Ran for 95550 frames Cell Events: 3092 mean_circ: 0.941162
Ran for 96050 frames Cell Events: 3122 mean_circ: 0.941091
Ran for 96550 frames Cell Events: 3138 mean_circ: 0.941149
Ran for 97550 frames Cell Events: 3189 mean_circ: 0.940995
Ran for 98550 frames Cell Events: 3218 mean_circ: 0.940884
Ran for 99050 frames Cell Events: 3231 mean_circ: 0.940877
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Ran for 100050 frames Cell Events: 3252 mean_circ: 0.94085
Ran for 101050 frames Cell Events: 3287 mean_circ: 0.940843
Ran for 102050 frames Cell Events: 3313 mean_circ: 0.940792
Ran for 103050 frames Cell Events: 3346 mean_circ: 0.940779
Ran for 104550 frames Cell Events: 3385 mean_circ: 0.940842
Ran for 105050 frames Cell Events: 3400 mean_circ: 0.940816
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Ran for 106050 frames Cell Events: 3434 mean_circ: 0.940844
Ran for 106550 frames Cell Events: 3447 mean_circ: 0.940859
Ran for 107050 frames Cell Events: 3463 mean_circ: 0.940877
Ran for 107550 frames Cell Events: 3470 mean_circ: 0.940872
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Ran for 120550 frames Cell Events: 3860 mean_circ: 0.940992
Ran for 121050 frames Cell Events: 3876 mean_circ: 0.940988
Ran for 122050 frames Cell Events: 3906 mean_circ: 0.941105
Ran for 123050 frames Cell Events: 3944 mean_circ: 0.941072
Ran for 123550 frames Cell Events: 3954 mean_circ: 0.941012
Ran for 124550 frames Cell Events: 3989 mean_circ: 0.940998
Ran for 125050 frames Cell Events: 4003 mean_circ: 0.941005
Ran for 126550 frames Cell Events: 4051 mean_circ: 0.941135
Ran for 127050 frames Cell Events: 4070 mean_circ: 0.941158
Ran for 127550 frames Cell Events: 4107 mean_circ: 0.941118
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Ran for 132050 frames Cell Events: 4295 mean_circ: 0.941283
Ran for 133050 frames Cell Events: 4325 mean_circ: 0.941292
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Ran for 142050 frames Cell Events: 4569 mean_circ: 0.941434
Ran for 142550 frames Cell Events: 4590 mean_circ: 0.941481
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Ran for 144550 frames Cell Events: 4665 mean_circ: 0.941385
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Ran for 203550 frames Cell Events: 6682 mean_circ: 0.941526
Ran for 205050 frames Cell Events: 6750 mean_circ: 0.941535
Ran for 205550 frames Cell Events: 6763 mean_circ: 0.941561
Ran for 206050 frames Cell Events: 6776 mean_circ: 0.941526
Ran for 206550 frames Cell Events: 6797 mean_circ: 0.941509
Ran for 207050 frames Cell Events: 6813 mean_circ: 0.941563
Ran for 207550 frames Cell Events: 6826 mean_circ: 0.941558
Ran for 208050 frames Cell Events: 6843 mean_circ: 0.941553
Ran for 208550 frames Cell Events: 6864 mean_circ: 0.941541
Ran for 209050 frames Cell Events: 6889 mean_circ: 0.941501
Ran for 210550 frames Cell Events: 6947 mean_circ: 0.941354
Ran for 211050 frames Cell Events: 6963 mean_circ: 0.941366
Ran for 213550 frames Cell Events: 7051 mean_circ: 0.941428
Ran for 214550 frames Cell Events: 7082 mean_circ: 0.941455
Ran for 215050 frames Cell Events: 7093 mean_circ: 0.941446
Ran for 216050 frames Cell Events: 7129 mean_circ: 0.941448
Ran for 217050 frames Cell Events: 7157 mean_circ: 0.941469
Ran for 218550 frames Cell Events: 7214 mean_circ: 0.941435
Ran for 219550 frames Cell Events: 7254 mean_circ: 0.941445
Ran for 220050 frames Cell Events: 7270 mean_circ: 0.941437
Ran for 220550 frames Cell Events: 7284 mean_circ: 0.941398
Ran for 221550 frames Cell Events: 7322 mean_circ: 0.941408
Ran for 222550 frames Cell Events: 7359 mean_circ: 0.941396
Ran for 223050 frames Cell Events: 7373 mean_circ: 0.941416
Ran for 224050 frames Cell Events: 7411 mean_circ: 0.941415
Ran for 224550 frames Cell Events: 7425 mean_circ: 0.9414
Ran for 225050 frames Cell Events: 7445 mean_circ: 0.941369
Ran for 225550 frames Cell Events: 7456 mean_circ: 0.941375
Ran for 226050 frames Cell Events: 7466 mean_circ: 0.941392
Ran for 227050 frames Cell Events: 7495 mean_circ: 0.941379
Ran for 227550 frames Cell Events: 7508 mean_circ: 0.94136
Ran for 228050 frames Cell Events: 7522 mean_circ: 0.941339
Ran for 229050 frames Cell Events: 7559 mean_circ: 0.941373
Ran for 230050 frames Cell Events: 7595 mean_circ: 0.941354
Ran for 231050 frames Cell Events: 7632 mean_circ: 0.941369
Ran for 232050 frames Cell Events: 7661 mean_circ: 0.941324
Ran for 232550 frames Cell Events: 7673 mean_circ: 0.941333
Ran for 235550 frames Cell Events: 7772 mean_circ: 0.941357
Ran for 236050 frames Cell Events: 7790 mean_circ: 0.941381
Ran for 237050 frames Cell Events: 7825 mean_circ: 0.941351
Ran for 237550 frames Cell Events: 7842 mean_circ: 0.941354
Ran for 239050 frames Cell Events: 7889 mean_circ: 0.941386
Ran for 240550 frames Cell Events: 7940 mean_circ: 0.94142
Ran for 241550 frames Cell Events: 7980 mean_circ: 0.941483
Ran for 243050 frames Cell Events: 8048 mean_circ: 0.941497
End this measurment..
Stopping time: 2.18022e+009
****************************************
Measurement finished
Mean Circularitiy: 0.941485
Mean Area: 666.485
Mean FL-1 intensity: 3687.03
Mean FL-2 intensity: 4166.31
Mean FL-3 intensity: 0
frames processed: 243095
Total Time: 122313ms
Number of Runs (new bg img) 0
Frames collected: 244523
Skipped frames: 25542
Rescued Frames: 2227921
Samples DAQ: 61130958
Samples written: 122261198
Samples per Channel: 61130599
FPS: 1999.16
Samples per second: 499788
Expected samples/s: 500000
Events: 8109
Events per second: 66.297
jumped back (PMT): 5001
#Frames read, frequency
# 0 frames :358800
# 1 frames :110967
# 2 frames :4725
# 3 frames :2215
# 4 frames :1209
# 5 frames :717
# 6 frames :664
# 7 frames :1708
# 8 frames :1177
# 9 frames :805
# 10 frames :934
# 11 frames :817
# 12 frames :657
# 13 frames :473
# 14 frames :300
# 15 frames :263
# 16 frames :204
# 17 frames :190
# 18 frames :187
# 19 frames :170
# 20 frames :131
# 21 frames :116
# 22 frames :84
# 23 frames :74
# 24 frames :58
# 25 frames :47
# 26 frames :39
# 27 frames :37
# 28 frames :26
# 29 frames :34
# 30 frames :21
# 31 frames :20
# 32 frames :23
# 33 frames :15
# 34 frames :13
# 35 frames :14
# 36 frames :9
# 37 frames :6
# 38 frames :5
# 39 frames :11
# 40 frames :6
# 41 frames :8
# 42 frames :9
# 43 frames :7
# 44 frames :3
# 45 frames :4
# 46 frames :4
# 47 frames :1
# 48 frames :1
# 50 frames :2
# 51 frames :3
# 52 frames :1
# 53 frames :2
# 54 frames :1
# 56 frames :2
# 57 frames :1
# 58 frames :4
# 59 frames :1
# 61 frames :1
# 63 frames :1
# 64 frames :1
# 65 frames :1
# 69 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2508
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0