M1_20170609_1506_dsRNA_Y6_col.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_15.49.51
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2870ded6ae40dbe4465771663a7e1734",
"name": "M1_20170609_1506_dsRNA_Y6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "13:50:13"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarningin dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8401 (max 8402) in '8401'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.5206
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.18418e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 7 mean_circ: 0.93319
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Ran for 378050 frames Cell Events: 6617 mean_circ: 0.925939
Ran for 378550 frames Cell Events: 6629 mean_circ: 0.925993
Ran for 379050 frames Cell Events: 6637 mean_circ: 0.926014
Ran for 380050 frames Cell Events: 6655 mean_circ: 0.926023
Ran for 381050 frames Cell Events: 6669 mean_circ: 0.925994
Ran for 382050 frames Cell Events: 6689 mean_circ: 0.926029
Ran for 382550 frames Cell Events: 6698 mean_circ: 0.926008
Ran for 383050 frames Cell Events: 6708 mean_circ: 0.925984
Ran for 383550 frames Cell Events: 6716 mean_circ: 0.925928
Ran for 384050 frames Cell Events: 6729 mean_circ: 0.925911
Ran for 384550 frames Cell Events: 6737 mean_circ: 0.925884
Ran for 385050 frames Cell Events: 6751 mean_circ: 0.92586
Ran for 385550 frames Cell Events: 6756 mean_circ: 0.925871
Ran for 386050 frames Cell Events: 6765 mean_circ: 0.925892
Ran for 386550 frames Cell Events: 6774 mean_circ: 0.925936
Ran for 387050 frames Cell Events: 6776 mean_circ: 0.925936
Ran for 387550 frames Cell Events: 6785 mean_circ: 0.925943
Ran for 388050 frames Cell Events: 6799 mean_circ: 0.925941
Ran for 388550 frames Cell Events: 6813 mean_circ: 0.925952
Ran for 389050 frames Cell Events: 6823 mean_circ: 0.926004
Ran for 389550 frames Cell Events: 6830 mean_circ: 0.925945
Ran for 390050 frames Cell Events: 6834 mean_circ: 0.925953
Ran for 390550 frames Cell Events: 6842 mean_circ: 0.925961
Ran for 391550 frames Cell Events: 6858 mean_circ: 0.925967
Ran for 392050 frames Cell Events: 6863 mean_circ: 0.92598
Ran for 393050 frames Cell Events: 6882 mean_circ: 0.926
Ran for 394050 frames Cell Events: 6898 mean_circ: 0.925998
Ran for 394550 frames Cell Events: 6899 mean_circ: 0.925995
Ran for 395050 frames Cell Events: 6904 mean_circ: 0.925991
Ran for 396050 frames Cell Events: 6931 mean_circ: 0.926073
Ran for 396550 frames Cell Events: 6941 mean_circ: 0.92609
Ran for 397050 frames Cell Events: 6951 mean_circ: 0.926089
Ran for 397550 frames Cell Events: 6956 mean_circ: 0.92609
Ran for 398050 frames Cell Events: 6961 mean_circ: 0.926082
Ran for 398550 frames Cell Events: 6971 mean_circ: 0.926069
Ran for 399050 frames Cell Events: 6982 mean_circ: 0.926052
Ran for 400050 frames Cell Events: 7005 mean_circ: 0.926028
Ran for 400550 frames Cell Events: 7013 mean_circ: 0.926008
Ran for 401050 frames Cell Events: 7018 mean_circ: 0.926022
Ran for 401550 frames Cell Events: 7021 mean_circ: 0.925999
Ran for 402050 frames Cell Events: 7032 mean_circ: 0.925995
Ran for 402550 frames Cell Events: 7045 mean_circ: 0.926016
Ran for 403550 frames Cell Events: 7058 mean_circ: 0.926036
Ran for 404050 frames Cell Events: 7070 mean_circ: 0.926021
Ran for 404550 frames Cell Events: 7081 mean_circ: 0.926045
Ran for 405550 frames Cell Events: 7100 mean_circ: 0.926019
Ran for 406050 frames Cell Events: 7104 mean_circ: 0.92602
Ran for 406550 frames Cell Events: 7110 mean_circ: 0.92603
Ran for 408050 frames Cell Events: 7131 mean_circ: 0.925979
Ran for 409050 frames Cell Events: 7155 mean_circ: 0.925914
Ran for 410050 frames Cell Events: 7168 mean_circ: 0.925929
Ran for 410550 frames Cell Events: 7176 mean_circ: 0.925944
Ran for 411050 frames Cell Events: 7180 mean_circ: 0.925929
Ran for 411550 frames Cell Events: 7190 mean_circ: 0.925899
Ran for 412050 frames Cell Events: 7200 mean_circ: 0.925892
Ran for 412550 frames Cell Events: 7207 mean_circ: 0.925863
Ran for 413550 frames Cell Events: 7221 mean_circ: 0.925881
Ran for 414050 frames Cell Events: 7227 mean_circ: 0.925876
Ran for 414550 frames Cell Events: 7235 mean_circ: 0.92591
Ran for 415050 frames Cell Events: 7241 mean_circ: 0.925898
Ran for 416050 frames Cell Events: 7258 mean_circ: 0.925882
Ran for 416550 frames Cell Events: 7269 mean_circ: 0.925881
Ran for 417050 frames Cell Events: 7278 mean_circ: 0.925886
Ran for 417550 frames Cell Events: 7288 mean_circ: 0.925893
Ran for 418050 frames Cell Events: 7297 mean_circ: 0.925858
Ran for 418550 frames Cell Events: 7305 mean_circ: 0.925861
Ran for 419050 frames Cell Events: 7319 mean_circ: 0.925841
Ran for 420050 frames Cell Events: 7338 mean_circ: 0.925805
Ran for 420550 frames Cell Events: 7347 mean_circ: 0.925789
Ran for 422050 frames Cell Events: 7378 mean_circ: 0.925767
Ran for 423050 frames Cell Events: 7389 mean_circ: 0.925784
Ran for 423550 frames Cell Events: 7399 mean_circ: 0.925813
Ran for 424550 frames Cell Events: 7410 mean_circ: 0.925805
Ran for 425050 frames Cell Events: 7419 mean_circ: 0.925821
Ran for 426050 frames Cell Events: 7430 mean_circ: 0.925831
Ran for 426550 frames Cell Events: 7439 mean_circ: 0.92584
Ran for 427050 frames Cell Events: 7446 mean_circ: 0.925842
Ran for 427550 frames Cell Events: 7459 mean_circ: 0.925874
Ran for 429050 frames Cell Events: 7485 mean_circ: 0.925768
Ran for 430050 frames Cell Events: 7509 mean_circ: 0.925751
Ran for 430550 frames Cell Events: 7514 mean_circ: 0.925766
Ran for 431050 frames Cell Events: 7519 mean_circ: 0.925772
Ran for 431550 frames Cell Events: 7528 mean_circ: 0.925769
Ran for 432050 frames Cell Events: 7535 mean_circ: 0.925734
Ran for 433050 frames Cell Events: 7552 mean_circ: 0.925669
Ran for 433550 frames Cell Events: 7557 mean_circ: 0.925663
Ran for 434050 frames Cell Events: 7559 mean_circ: 0.925671
Ran for 434550 frames Cell Events: 7565 mean_circ: 0.925671
Ran for 435050 frames Cell Events: 7574 mean_circ: 0.925672
Ran for 435550 frames Cell Events: 7581 mean_circ: 0.92568
Ran for 436050 frames Cell Events: 7591 mean_circ: 0.925676
Ran for 436550 frames Cell Events: 7602 mean_circ: 0.925685
Ran for 437050 frames Cell Events: 7607 mean_circ: 0.925697
Ran for 437550 frames Cell Events: 7614 mean_circ: 0.92568
Ran for 438050 frames Cell Events: 7623 mean_circ: 0.92569
Ran for 438550 frames Cell Events: 7631 mean_circ: 0.925697
Ran for 439050 frames Cell Events: 7644 mean_circ: 0.925683
Ran for 439550 frames Cell Events: 7653 mean_circ: 0.925668
Ran for 440050 frames Cell Events: 7658 mean_circ: 0.925674
Ran for 440550 frames Cell Events: 7667 mean_circ: 0.925676
Ran for 441050 frames Cell Events: 7674 mean_circ: 0.925647
Ran for 441550 frames Cell Events: 7690 mean_circ: 0.925665
Ran for 442050 frames Cell Events: 7694 mean_circ: 0.925665
Ran for 442550 frames Cell Events: 7702 mean_circ: 0.925681
Ran for 443050 frames Cell Events: 7714 mean_circ: 0.925608
Ran for 443550 frames Cell Events: 7720 mean_circ: 0.925604
Ran for 444050 frames Cell Events: 7727 mean_circ: 0.925591
Ran for 444550 frames Cell Events: 7732 mean_circ: 0.925584
Ran for 445050 frames Cell Events: 7738 mean_circ: 0.925558
Ran for 445550 frames Cell Events: 7752 mean_circ: 0.925567
Ran for 446050 frames Cell Events: 7760 mean_circ: 0.925596
Ran for 446550 frames Cell Events: 7770 mean_circ: 0.925617
Ran for 447050 frames Cell Events: 7776 mean_circ: 0.925624
Ran for 447550 frames Cell Events: 7787 mean_circ: 0.925628
Ran for 448550 frames Cell Events: 7813 mean_circ: 0.925677
Ran for 449050 frames Cell Events: 7820 mean_circ: 0.925689
Ran for 450050 frames Cell Events: 7844 mean_circ: 0.925698
Ran for 450550 frames Cell Events: 7850 mean_circ: 0.925695
Ran for 451050 frames Cell Events: 7857 mean_circ: 0.92569
Ran for 451550 frames Cell Events: 7869 mean_circ: 0.92569
Ran for 452050 frames Cell Events: 7878 mean_circ: 0.925699
Ran for 452550 frames Cell Events: 7884 mean_circ: 0.92569
Ran for 453050 frames Cell Events: 7890 mean_circ: 0.925665
Ran for 453550 frames Cell Events: 7899 mean_circ: 0.92566
Ran for 454050 frames Cell Events: 7911 mean_circ: 0.925611
Ran for 455050 frames Cell Events: 7928 mean_circ: 0.925598
Ran for 456050 frames Cell Events: 7949 mean_circ: 0.925567
Ran for 457050 frames Cell Events: 7967 mean_circ: 0.925563
Ran for 457550 frames Cell Events: 7973 mean_circ: 0.925576
Ran for 458050 frames Cell Events: 7981 mean_circ: 0.925591
Ran for 458550 frames Cell Events: 7987 mean_circ: 0.92559
Ran for 459050 frames Cell Events: 7992 mean_circ: 0.925582
Ran for 459550 frames Cell Events: 7996 mean_circ: 0.925544
Ran for 460050 frames Cell Events: 8007 mean_circ: 0.925551
Ran for 460550 frames Cell Events: 8020 mean_circ: 0.925517
Ran for 461050 frames Cell Events: 8032 mean_circ: 0.925547
Ran for 461550 frames Cell Events: 8039 mean_circ: 0.925551
Ran for 462050 frames Cell Events: 8051 mean_circ: 0.925505
Ran for 462550 frames Cell Events: 8063 mean_circ: 0.925531
Ran for 465050 frames Cell Events: 8122 mean_circ: 0.925527
Ran for 465550 frames Cell Events: 8127 mean_circ: 0.925506
Ran for 466050 frames Cell Events: 8135 mean_circ: 0.925528
Ran for 466550 frames Cell Events: 8142 mean_circ: 0.925508
Ran for 467050 frames Cell Events: 8150 mean_circ: 0.925481
Ran for 467550 frames Cell Events: 8157 mean_circ: 0.925485
Ran for 468050 frames Cell Events: 8165 mean_circ: 0.925465
Ran for 468550 frames Cell Events: 8173 mean_circ: 0.925464
Ran for 469050 frames Cell Events: 8187 mean_circ: 0.925444
Ran for 469550 frames Cell Events: 8199 mean_circ: 0.92542
Ran for 470050 frames Cell Events: 8212 mean_circ: 0.925398
Ran for 470550 frames Cell Events: 8228 mean_circ: 0.925388
Ran for 471550 frames Cell Events: 8249 mean_circ: 0.925403
Ran for 472050 frames Cell Events: 8260 mean_circ: 0.925432
Ran for 472550 frames Cell Events: 8272 mean_circ: 0.925434
Ran for 473050 frames Cell Events: 8279 mean_circ: 0.92544
Ran for 474050 frames Cell Events: 8306 mean_circ: 0.925456
Ran for 474550 frames Cell Events: 8317 mean_circ: 0.925419
Ran for 475050 frames Cell Events: 8328 mean_circ: 0.925425
Ran for 475550 frames Cell Events: 8335 mean_circ: 0.925401
Ran for 476550 frames Cell Events: 8359 mean_circ: 0.925406
Ran for 477050 frames Cell Events: 8371 mean_circ: 0.925432
Ran for 477550 frames Cell Events: 8379 mean_circ: 0.925403
Ran for 478550 frames Cell Events: 8395 mean_circ: 0.925451
End this measurment..
Stopping time: 2.18442e+009
****************************************
Measurement finished
Mean Circularitiy: 0.925425
Mean Area: 729.296
Mean FL-1 intensity: 3402.92
Mean FL-2 intensity: 4801.94
Mean FL-3 intensity: 0
frames processed: 475627
Total Time: 239498ms
Number of Runs (new bg img) 0
Frames collected: 478851
Skipped frames: 39375
Rescued Frames: 3425932
Samples DAQ: 119712737
Samples written: 239425308
Samples per Channel: 119712654
FPS: 1999.39
Samples per second: 499848
Expected samples/s: 500000
Events: 8402
Events per second: 35.0817
jumped back (PMT): 8404
#Frames read, frequency
# 0 frames :622280
# 1 frames :304894
# 2 frames :10392
# 3 frames :3943
# 4 frames :2411
# 5 frames :1101
# 6 frames :1158
# 7 frames :2811
# 8 frames :1224
# 9 frames :942
# 10 frames :1967
# 11 frames :1875
# 12 frames :1123
# 13 frames :558
# 14 frames :212
# 15 frames :177
# 16 frames :171
# 17 frames :124
# 18 frames :88
# 19 frames :53
# 20 frames :38
# 21 frames :23
# 22 frames :19
# 23 frames :30
# 24 frames :17
# 25 frames :11
# 26 frames :4
# 27 frames :7
# 28 frames :6
# 29 frames :5
# 30 frames :2
# 31 frames :5
# 33 frames :1
# 35 frames :3
# 54 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.5206
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 3a72062f3a6225faaecad0bdce192946 |
format | RT-FDC |
id | f4c09c2b-fcb7-4b20-aa06-bc375d752fc1 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | daa49a50-6a99-41ad-8f22-2eb9b5734653 |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/f4c/09c/2b-fcb7-4b20-aa06-bc375d752fc1 |
sha256 | a29b2d858411e97ac2c7123d0f1bfda6c891d8db3045b5c4b9fb2dcfc28cd859 |
size | 148.5 MiB |
dc:experiment:date | 2017-06-09 |
dc:experiment:event count | 8,400 |
dc:experiment:run identifier | None-bb5a |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170609_1506_dsRNA_Y6_col |
dc:experiment:time | 15:06:09 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |