Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_15.50.20
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "322ebd2be5b63ed48ae9501b91323df3",
"name": "M1_20170609_1510_dsRNA_Y6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "13:50:39"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8057 (max 8058) in '8057'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.5616
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.18447e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 30 mean_circ: 0.920042
Ran for 3050 frames Cell Events: 44 mean_circ: 0.917813
Ran for 3550 frames Cell Events: 53 mean_circ: 0.91938
Ran for 4050 frames Cell Events: 61 mean_circ: 0.918069
Ran for 4550 frames Cell Events: 67 mean_circ: 0.916772
Ran for 5050 frames Cell Events: 80 mean_circ: 0.918264
Ran for 5550 frames Cell Events: 86 mean_circ: 0.919157
Ran for 7050 frames Cell Events: 115 mean_circ: 0.919166
Ran for 7550 frames Cell Events: 119 mean_circ: 0.920061
Ran for 8050 frames Cell Events: 130 mean_circ: 0.920348
Ran for 8550 frames Cell Events: 144 mean_circ: 0.919939
Ran for 9550 frames Cell Events: 158 mean_circ: 0.922275
Ran for 10550 frames Cell Events: 175 mean_circ: 0.921778
Ran for 11550 frames Cell Events: 196 mean_circ: 0.920439
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Ran for 13550 frames Cell Events: 224 mean_circ: 0.919812
Ran for 14550 frames Cell Events: 239 mean_circ: 0.919625
Ran for 15050 frames Cell Events: 248 mean_circ: 0.9202
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Ran for 17050 frames Cell Events: 287 mean_circ: 0.920253
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Ran for 18050 frames Cell Events: 299 mean_circ: 0.921441
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Ran for 23550 frames Cell Events: 393 mean_circ: 0.921991
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Ran for 36050 frames Cell Events: 602 mean_circ: 0.917932
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Ran for 37550 frames Cell Events: 631 mean_circ: 0.91766
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Ran for 39050 frames Cell Events: 660 mean_circ: 0.917269
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Ran for 41050 frames Cell Events: 707 mean_circ: 0.916664
Ran for 42050 frames Cell Events: 724 mean_circ: 0.916837
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Ran for 63050 frames Cell Events: 1102 mean_circ: 0.917223
Ran for 63550 frames Cell Events: 1106 mean_circ: 0.917192
Ran for 64050 frames Cell Events: 1115 mean_circ: 0.917307
Ran for 64550 frames Cell Events: 1121 mean_circ: 0.91709
Ran for 65550 frames Cell Events: 1140 mean_circ: 0.916989
Ran for 66050 frames Cell Events: 1145 mean_circ: 0.917086
Ran for 66550 frames Cell Events: 1155 mean_circ: 0.917217
Ran for 67550 frames Cell Events: 1163 mean_circ: 0.917404
Ran for 68550 frames Cell Events: 1180 mean_circ: 0.917401
Ran for 70050 frames Cell Events: 1210 mean_circ: 0.917056
Ran for 71050 frames Cell Events: 1224 mean_circ: 0.916978
Ran for 71550 frames Cell Events: 1234 mean_circ: 0.917185
Ran for 72050 frames Cell Events: 1242 mean_circ: 0.91739
Ran for 73050 frames Cell Events: 1260 mean_circ: 0.917703
Ran for 73550 frames Cell Events: 1268 mean_circ: 0.917773
Ran for 74050 frames Cell Events: 1274 mean_circ: 0.917685
Ran for 74550 frames Cell Events: 1282 mean_circ: 0.917648
Ran for 75050 frames Cell Events: 1289 mean_circ: 0.917614
Ran for 75550 frames Cell Events: 1301 mean_circ: 0.917581
Ran for 76050 frames Cell Events: 1308 mean_circ: 0.917581
Ran for 76550 frames Cell Events: 1312 mean_circ: 0.917663
Ran for 77050 frames Cell Events: 1318 mean_circ: 0.917729
Ran for 77550 frames Cell Events: 1322 mean_circ: 0.917756
Ran for 78050 frames Cell Events: 1327 mean_circ: 0.917622
Ran for 79050 frames Cell Events: 1344 mean_circ: 0.91784
Ran for 79550 frames Cell Events: 1348 mean_circ: 0.917974
Ran for 80050 frames Cell Events: 1356 mean_circ: 0.917794
Ran for 81550 frames Cell Events: 1381 mean_circ: 0.917829
Ran for 82550 frames Cell Events: 1398 mean_circ: 0.917749
Ran for 83050 frames Cell Events: 1406 mean_circ: 0.917768
Ran for 84050 frames Cell Events: 1421 mean_circ: 0.917959
Ran for 84550 frames Cell Events: 1440 mean_circ: 0.917896
Ran for 85050 frames Cell Events: 1448 mean_circ: 0.917904
Ran for 86050 frames Cell Events: 1461 mean_circ: 0.917467
Ran for 87050 frames Cell Events: 1476 mean_circ: 0.917301
Ran for 87550 frames Cell Events: 1481 mean_circ: 0.917204
Ran for 88550 frames Cell Events: 1511 mean_circ: 0.916983
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Ran for 90050 frames Cell Events: 1532 mean_circ: 0.916764
Ran for 90550 frames Cell Events: 1543 mean_circ: 0.916867
Ran for 91050 frames Cell Events: 1552 mean_circ: 0.916793
Ran for 91550 frames Cell Events: 1565 mean_circ: 0.916837
Ran for 92050 frames Cell Events: 1570 mean_circ: 0.916936
Ran for 92550 frames Cell Events: 1578 mean_circ: 0.916593
Ran for 93050 frames Cell Events: 1582 mean_circ: 0.916525
Ran for 93550 frames Cell Events: 1586 mean_circ: 0.916439
Ran for 94550 frames Cell Events: 1601 mean_circ: 0.916542
Ran for 95050 frames Cell Events: 1610 mean_circ: 0.916562
Ran for 96050 frames Cell Events: 1624 mean_circ: 0.91635
Ran for 97050 frames Cell Events: 1643 mean_circ: 0.916172
Ran for 97550 frames Cell Events: 1650 mean_circ: 0.916189
Ran for 98550 frames Cell Events: 1663 mean_circ: 0.915772
Ran for 99050 frames Cell Events: 1676 mean_circ: 0.915658
Ran for 100050 frames Cell Events: 1690 mean_circ: 0.915588
Ran for 100550 frames Cell Events: 1698 mean_circ: 0.915678
Ran for 101550 frames Cell Events: 1713 mean_circ: 0.915651
Ran for 102050 frames Cell Events: 1722 mean_circ: 0.915504
Ran for 102550 frames Cell Events: 1728 mean_circ: 0.915385
Ran for 104050 frames Cell Events: 1760 mean_circ: 0.915384
Ran for 105550 frames Cell Events: 1790 mean_circ: 0.915267
Ran for 106050 frames Cell Events: 1802 mean_circ: 0.915359
Ran for 106550 frames Cell Events: 1814 mean_circ: 0.915186
Ran for 107050 frames Cell Events: 1829 mean_circ: 0.915021
Ran for 107550 frames Cell Events: 1843 mean_circ: 0.914834
Ran for 108050 frames Cell Events: 1849 mean_circ: 0.914823
Ran for 109050 frames Cell Events: 1863 mean_circ: 0.914741
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Ran for 110550 frames Cell Events: 1882 mean_circ: 0.914761
Ran for 111050 frames Cell Events: 1896 mean_circ: 0.914861
Ran for 111550 frames Cell Events: 1900 mean_circ: 0.914884
Ran for 112050 frames Cell Events: 1910 mean_circ: 0.914806
Ran for 112550 frames Cell Events: 1917 mean_circ: 0.914875
Ran for 113550 frames Cell Events: 1937 mean_circ: 0.915016
Ran for 115050 frames Cell Events: 1974 mean_circ: 0.914817
Ran for 115550 frames Cell Events: 1982 mean_circ: 0.914733
Ran for 116050 frames Cell Events: 1995 mean_circ: 0.914559
Ran for 117050 frames Cell Events: 2023 mean_circ: 0.914479
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Ran for 118550 frames Cell Events: 2058 mean_circ: 0.91458
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Ran for 120050 frames Cell Events: 2078 mean_circ: 0.914612
Ran for 120550 frames Cell Events: 2079 mean_circ: 0.914612
Ran for 121050 frames Cell Events: 2086 mean_circ: 0.914577
Ran for 121550 frames Cell Events: 2097 mean_circ: 0.914599
Ran for 122550 frames Cell Events: 2106 mean_circ: 0.914529
Ran for 123050 frames Cell Events: 2113 mean_circ: 0.914597
Ran for 123550 frames Cell Events: 2119 mean_circ: 0.914612
Ran for 124050 frames Cell Events: 2126 mean_circ: 0.914576
Ran for 124550 frames Cell Events: 2138 mean_circ: 0.914264
Ran for 125050 frames Cell Events: 2146 mean_circ: 0.914235
Ran for 125550 frames Cell Events: 2156 mean_circ: 0.914181
Ran for 127050 frames Cell Events: 2187 mean_circ: 0.914109
Ran for 127550 frames Cell Events: 2197 mean_circ: 0.914015
Ran for 128550 frames Cell Events: 2211 mean_circ: 0.914034
Ran for 129050 frames Cell Events: 2218 mean_circ: 0.91394
Ran for 130550 frames Cell Events: 2247 mean_circ: 0.913908
Ran for 131050 frames Cell Events: 2251 mean_circ: 0.913881
Ran for 131550 frames Cell Events: 2262 mean_circ: 0.913943
Ran for 132050 frames Cell Events: 2267 mean_circ: 0.913991
Ran for 132550 frames Cell Events: 2276 mean_circ: 0.913999
Ran for 133050 frames Cell Events: 2282 mean_circ: 0.913938
Ran for 133550 frames Cell Events: 2292 mean_circ: 0.913941
Ran for 134050 frames Cell Events: 2297 mean_circ: 0.913982
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Ran for 138050 frames Cell Events: 2373 mean_circ: 0.913734
Ran for 139050 frames Cell Events: 2396 mean_circ: 0.913656
Ran for 139550 frames Cell Events: 2396 mean_circ: 0.913657
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Ran for 145550 frames Cell Events: 2502 mean_circ: 0.913572
Ran for 146050 frames Cell Events: 2509 mean_circ: 0.913551
Ran for 146550 frames Cell Events: 2513 mean_circ: 0.9135
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Ran for 156050 frames Cell Events: 2705 mean_circ: 0.913315
Ran for 156550 frames Cell Events: 2716 mean_circ: 0.913248
Ran for 157050 frames Cell Events: 2719 mean_circ: 0.913135
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Ran for 158050 frames Cell Events: 2733 mean_circ: 0.91301
Ran for 158550 frames Cell Events: 2742 mean_circ: 0.912947
Ran for 159050 frames Cell Events: 2756 mean_circ: 0.91284
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Ran for 160550 frames Cell Events: 2785 mean_circ: 0.912902
Ran for 162550 frames Cell Events: 2816 mean_circ: 0.91266
Ran for 163050 frames Cell Events: 2828 mean_circ: 0.912576
Ran for 163550 frames Cell Events: 2837 mean_circ: 0.912635
Ran for 164550 frames Cell Events: 2859 mean_circ: 0.912636
Ran for 165550 frames Cell Events: 2870 mean_circ: 0.912614
Ran for 166550 frames Cell Events: 2893 mean_circ: 0.912519
Ran for 167550 frames Cell Events: 2920 mean_circ: 0.912551
Ran for 168550 frames Cell Events: 2942 mean_circ: 0.912505
Ran for 169050 frames Cell Events: 2954 mean_circ: 0.912523
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Ran for 170050 frames Cell Events: 2981 mean_circ: 0.912475
Ran for 170550 frames Cell Events: 2985 mean_circ: 0.912451
Ran for 171050 frames Cell Events: 2993 mean_circ: 0.912385
Ran for 171550 frames Cell Events: 2997 mean_circ: 0.912403
Ran for 172050 frames Cell Events: 3006 mean_circ: 0.912417
Ran for 173550 frames Cell Events: 3027 mean_circ: 0.912531
Ran for 174050 frames Cell Events: 3037 mean_circ: 0.912541
Ran for 174550 frames Cell Events: 3047 mean_circ: 0.912532
Ran for 175550 frames Cell Events: 3069 mean_circ: 0.912599
Ran for 176050 frames Cell Events: 3079 mean_circ: 0.912657
Ran for 177050 frames Cell Events: 3106 mean_circ: 0.912389
Ran for 177550 frames Cell Events: 3115 mean_circ: 0.912457
Ran for 178050 frames Cell Events: 3128 mean_circ: 0.912452
Ran for 178550 frames Cell Events: 3136 mean_circ: 0.912361
Ran for 179050 frames Cell Events: 3142 mean_circ: 0.912396
Ran for 179550 frames Cell Events: 3149 mean_circ: 0.91241
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Ran for 417050 frames Cell Events: 7777 mean_circ: 0.910321
Ran for 417550 frames Cell Events: 7783 mean_circ: 0.910344
Ran for 419550 frames Cell Events: 7833 mean_circ: 0.910255
Ran for 420050 frames Cell Events: 7843 mean_circ: 0.910255
Ran for 420550 frames Cell Events: 7857 mean_circ: 0.910273
Ran for 421050 frames Cell Events: 7870 mean_circ: 0.910261
Ran for 422050 frames Cell Events: 7891 mean_circ: 0.910224
Ran for 423050 frames Cell Events: 7907 mean_circ: 0.910225
Ran for 423550 frames Cell Events: 7914 mean_circ: 0.910214
Ran for 424050 frames Cell Events: 7922 mean_circ: 0.910224
Ran for 425550 frames Cell Events: 7955 mean_circ: 0.910251
Ran for 426050 frames Cell Events: 7969 mean_circ: 0.910238
Ran for 426550 frames Cell Events: 7986 mean_circ: 0.910186
Ran for 427050 frames Cell Events: 7993 mean_circ: 0.910176
Ran for 428050 frames Cell Events: 8015 mean_circ: 0.91019
Ran for 428550 frames Cell Events: 8031 mean_circ: 0.910181
Ran for 429050 frames Cell Events: 8036 mean_circ: 0.910166
Ran for 429550 frames Cell Events: 8043 mean_circ: 0.910207
Ran for 430050 frames Cell Events: 8055 mean_circ: 0.910234
End this measurment..
Stopping time: 2.18469e+009
****************************************
Measurement finished
Mean Circularitiy: 0.910219
Mean Area: 733.813
Mean FL-1 intensity: 3267.19
Mean FL-2 intensity: 5201.53
Mean FL-3 intensity: 0
frames processed: 430277
Total Time: 215250ms
Number of Runs (new bg img) 0
Frames collected: 430390
Skipped frames: 39488
Rescued Frames: 3578616
Samples DAQ: 107597600
Samples written: 215195034
Samples per Channel: 107597517
FPS: 1999.49
Samples per second: 499872
Expected samples/s: 500000
Events: 8058
Events per second: 37.4355
jumped back (PMT): 19
#Frames read, frequency
# 0 frames :583161
# 1 frames :249968
# 2 frames :8107
# 3 frames :3815
# 4 frames :1961
# 5 frames :1232
# 6 frames :1048
# 7 frames :2364
# 8 frames :1397
# 9 frames :971
# 10 frames :1217
# 11 frames :1325
# 12 frames :907
# 13 frames :598
# 14 frames :337
# 15 frames :292
# 16 frames :219
# 17 frames :267
# 18 frames :330
# 19 frames :294
# 20 frames :242
# 21 frames :209
# 22 frames :119
# 23 frames :104
# 24 frames :67
# 25 frames :48
# 26 frames :26
# 27 frames :22
# 28 frames :17
# 29 frames :15
# 30 frames :13
# 31 frames :10
# 32 frames :7
# 33 frames :10
# 34 frames :6
# 35 frames :4
# 36 frames :2
# 37 frames :6
# 38 frames :3
# 39 frames :2
# 40 frames :3
# 41 frames :1
# 42 frames :2
# 43 frames :2
# 44 frames :2
# 45 frames :1
# 49 frames :1
# 52 frames :1
# 61 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.5616
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0