Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.00.18
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e5dfe6935b303cca87f0704d3560d93e",
"name": "M1_20170611_1406_dsRNA_N5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:00:42"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9876 (max 9877) in '9876'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.804
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.35339e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.919345
Ran for 1050 frames Cell Events: 29 mean_circ: 0.927953
Ran for 1550 frames Cell Events: 44 mean_circ: 0.930056
Ran for 2050 frames Cell Events: 64 mean_circ: 0.931785
Ran for 2550 frames Cell Events: 83 mean_circ: 0.937418
Ran for 3050 frames Cell Events: 99 mean_circ: 0.935931
Ran for 3550 frames Cell Events: 114 mean_circ: 0.937264
Ran for 4550 frames Cell Events: 148 mean_circ: 0.935974
Ran for 5050 frames Cell Events: 163 mean_circ: 0.933301
Ran for 5550 frames Cell Events: 181 mean_circ: 0.933412
Ran for 6050 frames Cell Events: 192 mean_circ: 0.934035
Ran for 6550 frames Cell Events: 204 mean_circ: 0.934056
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Ran for 7550 frames Cell Events: 235 mean_circ: 0.935025
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Ran for 10550 frames Cell Events: 358 mean_circ: 0.937578
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Ran for 12050 frames Cell Events: 417 mean_circ: 0.936632
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Ran for 70050 frames Cell Events: 2515 mean_circ: 0.937029
Ran for 70550 frames Cell Events: 2536 mean_circ: 0.937067
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Ran for 72050 frames Cell Events: 2600 mean_circ: 0.937091
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Ran for 73050 frames Cell Events: 2646 mean_circ: 0.937076
Ran for 73550 frames Cell Events: 2655 mean_circ: 0.937064
Ran for 74050 frames Cell Events: 2672 mean_circ: 0.937015
Ran for 75050 frames Cell Events: 2704 mean_circ: 0.936958
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Ran for 76550 frames Cell Events: 2766 mean_circ: 0.936951
Ran for 77550 frames Cell Events: 2807 mean_circ: 0.936968
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Ran for 79550 frames Cell Events: 2866 mean_circ: 0.937027
Ran for 80050 frames Cell Events: 2890 mean_circ: 0.937004
Ran for 80550 frames Cell Events: 2905 mean_circ: 0.937015
Ran for 81050 frames Cell Events: 2928 mean_circ: 0.937056
Ran for 81550 frames Cell Events: 2944 mean_circ: 0.937016
Ran for 82050 frames Cell Events: 2960 mean_circ: 0.937029
Ran for 82550 frames Cell Events: 2982 mean_circ: 0.937084
Ran for 83550 frames Cell Events: 3024 mean_circ: 0.937042
Ran for 84050 frames Cell Events: 3041 mean_circ: 0.937034
Ran for 85050 frames Cell Events: 3093 mean_circ: 0.937128
Ran for 85550 frames Cell Events: 3116 mean_circ: 0.937081
Ran for 86050 frames Cell Events: 3142 mean_circ: 0.937066
Ran for 87050 frames Cell Events: 3177 mean_circ: 0.937081
Ran for 89050 frames Cell Events: 3241 mean_circ: 0.937151
Ran for 89550 frames Cell Events: 3255 mean_circ: 0.937179
Ran for 90550 frames Cell Events: 3297 mean_circ: 0.937139
Ran for 91050 frames Cell Events: 3310 mean_circ: 0.937164
Ran for 91550 frames Cell Events: 3330 mean_circ: 0.937176
Ran for 92550 frames Cell Events: 3372 mean_circ: 0.937219
Ran for 94050 frames Cell Events: 3427 mean_circ: 0.937204
Ran for 94550 frames Cell Events: 3456 mean_circ: 0.937305
Ran for 95050 frames Cell Events: 3468 mean_circ: 0.937337
Ran for 96050 frames Cell Events: 3510 mean_circ: 0.937307
Ran for 96550 frames Cell Events: 3528 mean_circ: 0.937328
Ran for 98050 frames Cell Events: 3572 mean_circ: 0.937277
Ran for 98550 frames Cell Events: 3588 mean_circ: 0.937281
Ran for 99550 frames Cell Events: 3620 mean_circ: 0.937307
Ran for 100550 frames Cell Events: 3659 mean_circ: 0.937208
Ran for 101550 frames Cell Events: 3699 mean_circ: 0.93718
Ran for 102050 frames Cell Events: 3719 mean_circ: 0.937125
Ran for 102550 frames Cell Events: 3742 mean_circ: 0.937118
Ran for 103050 frames Cell Events: 3757 mean_circ: 0.93708
Ran for 103550 frames Cell Events: 3771 mean_circ: 0.937109
Ran for 104050 frames Cell Events: 3785 mean_circ: 0.937133
Ran for 105050 frames Cell Events: 3815 mean_circ: 0.93716
Ran for 106050 frames Cell Events: 3852 mean_circ: 0.937118
Ran for 107050 frames Cell Events: 3882 mean_circ: 0.936972
Ran for 107550 frames Cell Events: 3895 mean_circ: 0.936979
Ran for 108550 frames Cell Events: 3923 mean_circ: 0.936845
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Ran for 110550 frames Cell Events: 3992 mean_circ: 0.936726
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Ran for 121050 frames Cell Events: 4360 mean_circ: 0.936777
Ran for 121550 frames Cell Events: 4375 mean_circ: 0.936827
Ran for 122550 frames Cell Events: 4413 mean_circ: 0.936897
Ran for 124050 frames Cell Events: 4464 mean_circ: 0.93683
Ran for 124550 frames Cell Events: 4482 mean_circ: 0.936836
Ran for 125550 frames Cell Events: 4512 mean_circ: 0.936828
Ran for 126050 frames Cell Events: 4533 mean_circ: 0.936846
Ran for 126550 frames Cell Events: 4544 mean_circ: 0.936798
Ran for 127550 frames Cell Events: 4570 mean_circ: 0.936802
Ran for 128050 frames Cell Events: 4581 mean_circ: 0.936745
Ran for 130050 frames Cell Events: 4649 mean_circ: 0.936655
Ran for 131050 frames Cell Events: 4684 mean_circ: 0.936715
Ran for 131550 frames Cell Events: 4710 mean_circ: 0.936717
Ran for 132050 frames Cell Events: 4736 mean_circ: 0.936685
Ran for 132550 frames Cell Events: 4757 mean_circ: 0.936655
Ran for 134050 frames Cell Events: 4821 mean_circ: 0.936614
Ran for 134550 frames Cell Events: 4841 mean_circ: 0.936585
Ran for 135050 frames Cell Events: 4867 mean_circ: 0.936573
Ran for 136050 frames Cell Events: 4908 mean_circ: 0.936616
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Ran for 139050 frames Cell Events: 5031 mean_circ: 0.936542
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Ran for 141050 frames Cell Events: 5103 mean_circ: 0.936519
Ran for 142050 frames Cell Events: 5133 mean_circ: 0.936462
Ran for 143550 frames Cell Events: 5188 mean_circ: 0.936299
Ran for 144050 frames Cell Events: 5212 mean_circ: 0.936286
Ran for 144550 frames Cell Events: 5242 mean_circ: 0.936363
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Ran for 145550 frames Cell Events: 5282 mean_circ: 0.936371
Ran for 146050 frames Cell Events: 5296 mean_circ: 0.936332
Ran for 146550 frames Cell Events: 5310 mean_circ: 0.93634
Ran for 147550 frames Cell Events: 5342 mean_circ: 0.936337
Ran for 148050 frames Cell Events: 5359 mean_circ: 0.936328
Ran for 148550 frames Cell Events: 5373 mean_circ: 0.936341
Ran for 150050 frames Cell Events: 5454 mean_circ: 0.936302
Ran for 151050 frames Cell Events: 5500 mean_circ: 0.936327
Ran for 152050 frames Cell Events: 5536 mean_circ: 0.936352
Ran for 152550 frames Cell Events: 5557 mean_circ: 0.936306
Ran for 153050 frames Cell Events: 5585 mean_circ: 0.936321
Ran for 153550 frames Cell Events: 5603 mean_circ: 0.936279
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Ran for 156050 frames Cell Events: 5703 mean_circ: 0.93622
Ran for 156550 frames Cell Events: 5718 mean_circ: 0.93618
Ran for 157050 frames Cell Events: 5732 mean_circ: 0.936179
Ran for 158550 frames Cell Events: 5789 mean_circ: 0.936206
Ran for 159050 frames Cell Events: 5816 mean_circ: 0.936216
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Ran for 164050 frames Cell Events: 6004 mean_circ: 0.936247
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Ran for 201550 frames Cell Events: 7475 mean_circ: 0.936105
Ran for 202050 frames Cell Events: 7496 mean_circ: 0.93611
Ran for 202550 frames Cell Events: 7515 mean_circ: 0.93613
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Ran for 206050 frames Cell Events: 7663 mean_circ: 0.936173
Ran for 207550 frames Cell Events: 7717 mean_circ: 0.936156
Ran for 208050 frames Cell Events: 7729 mean_circ: 0.936122
Ran for 209050 frames Cell Events: 7780 mean_circ: 0.936125
Ran for 210050 frames Cell Events: 7820 mean_circ: 0.936096
Ran for 210550 frames Cell Events: 7836 mean_circ: 0.936085
Ran for 211550 frames Cell Events: 7877 mean_circ: 0.936059
Ran for 212550 frames Cell Events: 7907 mean_circ: 0.936051
Ran for 213550 frames Cell Events: 7950 mean_circ: 0.936011
Ran for 214050 frames Cell Events: 7970 mean_circ: 0.936009
Ran for 214550 frames Cell Events: 7999 mean_circ: 0.935967
Ran for 215050 frames Cell Events: 8022 mean_circ: 0.935982
Ran for 215550 frames Cell Events: 8042 mean_circ: 0.936008
Ran for 216050 frames Cell Events: 8063 mean_circ: 0.936025
Ran for 216550 frames Cell Events: 8078 mean_circ: 0.936024
Ran for 217050 frames Cell Events: 8094 mean_circ: 0.936021
Ran for 218050 frames Cell Events: 8131 mean_circ: 0.936047
Ran for 218550 frames Cell Events: 8163 mean_circ: 0.936089
Ran for 219550 frames Cell Events: 8214 mean_circ: 0.936148
Ran for 220050 frames Cell Events: 8226 mean_circ: 0.936155
Ran for 221050 frames Cell Events: 8265 mean_circ: 0.936146
Ran for 221550 frames Cell Events: 8280 mean_circ: 0.93614
Ran for 222050 frames Cell Events: 8293 mean_circ: 0.936126
Ran for 222550 frames Cell Events: 8308 mean_circ: 0.93611
Ran for 223050 frames Cell Events: 8328 mean_circ: 0.93604
Ran for 223550 frames Cell Events: 8338 mean_circ: 0.936039
Ran for 225550 frames Cell Events: 8424 mean_circ: 0.935984
Ran for 226050 frames Cell Events: 8447 mean_circ: 0.936004
Ran for 226550 frames Cell Events: 8485 mean_circ: 0.936021
Ran for 227050 frames Cell Events: 8519 mean_circ: 0.935948
Ran for 228050 frames Cell Events: 8548 mean_circ: 0.935948
Ran for 228550 frames Cell Events: 8568 mean_circ: 0.935967
Ran for 229050 frames Cell Events: 8596 mean_circ: 0.935959
Ran for 230050 frames Cell Events: 8635 mean_circ: 0.935964
Ran for 230550 frames Cell Events: 8657 mean_circ: 0.935908
Ran for 231550 frames Cell Events: 8686 mean_circ: 0.935903
Ran for 232550 frames Cell Events: 8722 mean_circ: 0.935874
Ran for 233550 frames Cell Events: 8760 mean_circ: 0.9359
Ran for 234050 frames Cell Events: 8788 mean_circ: 0.935881
Ran for 234550 frames Cell Events: 8804 mean_circ: 0.935893
Ran for 235050 frames Cell Events: 8819 mean_circ: 0.935883
Ran for 237050 frames Cell Events: 8895 mean_circ: 0.935915
Ran for 237550 frames Cell Events: 8913 mean_circ: 0.935903
Ran for 238050 frames Cell Events: 8930 mean_circ: 0.935876
Ran for 238550 frames Cell Events: 8947 mean_circ: 0.935868
Ran for 239550 frames Cell Events: 8985 mean_circ: 0.935866
Ran for 240050 frames Cell Events: 9011 mean_circ: 0.935846
Ran for 240550 frames Cell Events: 9032 mean_circ: 0.935866
Ran for 242550 frames Cell Events: 9108 mean_circ: 0.935851
Ran for 243050 frames Cell Events: 9131 mean_circ: 0.935826
Ran for 243550 frames Cell Events: 9150 mean_circ: 0.935838
Ran for 244550 frames Cell Events: 9195 mean_circ: 0.935835
Ran for 246050 frames Cell Events: 9250 mean_circ: 0.935859
Ran for 247050 frames Cell Events: 9284 mean_circ: 0.93589
Ran for 248050 frames Cell Events: 9326 mean_circ: 0.935876
Ran for 249550 frames Cell Events: 9400 mean_circ: 0.935826
Ran for 252550 frames Cell Events: 9528 mean_circ: 0.935727
Ran for 253050 frames Cell Events: 9542 mean_circ: 0.935723
Ran for 253550 frames Cell Events: 9559 mean_circ: 0.935737
Ran for 254050 frames Cell Events: 9572 mean_circ: 0.935749
Ran for 254550 frames Cell Events: 9592 mean_circ: 0.93573
Ran for 255050 frames Cell Events: 9611 mean_circ: 0.935736
Ran for 256550 frames Cell Events: 9684 mean_circ: 0.935732
Ran for 257050 frames Cell Events: 9701 mean_circ: 0.935708
Ran for 258050 frames Cell Events: 9745 mean_circ: 0.935685
Ran for 259050 frames Cell Events: 9763 mean_circ: 0.935684
Ran for 260050 frames Cell Events: 9794 mean_circ: 0.935676
Ran for 261050 frames Cell Events: 9846 mean_circ: 0.935679
Ran for 261550 frames Cell Events: 9869 mean_circ: 0.935679
End this measurment..
Stopping time: 2.35352e+009
****************************************
Measurement finished
Mean Circularitiy: 0.93568
Mean Area: 613.751
Mean FL-1 intensity: 3391.34
Mean FL-2 intensity: 4664.15
Mean FL-3 intensity: 0
frames processed: 259437
Total Time: 131051ms
Number of Runs (new bg img) 0
Frames collected: 261894
Skipped frames: 10923
Rescued Frames: 734879
Samples DAQ: 65473843
Samples written: 130946826
Samples per Channel: 65473413
FPS: 1998.41
Samples per second: 499602
Expected samples/s: 500000
Events: 9877
Events per second: 75.3676
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :381055
# 1 frames :122872
# 2 frames :4993
# 3 frames :2071
# 4 frames :1120
# 5 frames :545
# 6 frames :2383
# 7 frames :1841
# 8 frames :1419
# 9 frames :838
# 10 frames :764
# 11 frames :759
# 12 frames :613
# 13 frames :366
# 14 frames :406
# 15 frames :270
# 16 frames :268
# 17 frames :206
# 18 frames :207
# 19 frames :134
# 20 frames :101
# 21 frames :93
# 22 frames :77
# 23 frames :52
# 24 frames :42
# 25 frames :27
# 26 frames :24
# 27 frames :23
# 28 frames :21
# 29 frames :19
# 30 frames :13
# 31 frames :21
# 32 frames :13
# 33 frames :13
# 34 frames :9
# 35 frames :9
# 36 frames :5
# 37 frames :3
# 38 frames :7
# 39 frames :8
# 40 frames :5
# 41 frames :1
# 42 frames :2
# 43 frames :2
# 45 frames :2
# 46 frames :4
# 47 frames :2
# 48 frames :1
# 50 frames :1
# 54 frames :1
# 55 frames :1
# 60 frames :1
# 75 frames :1
# 79 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.804
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0