M1_20170611_1630_dsRNA_N7_col.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.12.06
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "10eb2b7e558a84bb8b67fa25c23a19b7",
"name": "M1_20170611_1630_dsRNA_N7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:12:28"
}
}
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.939
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.36205e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.941033
Ran for 550 frames Cell Events: 6 mean_circ: 0.943851
Ran for 1050 frames Cell Events: 10 mean_circ: 0.937159
Ran for 1550 frames Cell Events: 17 mean_circ: 0.939582
Ran for 2050 frames Cell Events: 24 mean_circ: 0.945024
Ran for 2550 frames Cell Events: 28 mean_circ: 0.942423
Ran for 4050 frames Cell Events: 49 mean_circ: 0.943999
Ran for 4550 frames Cell Events: 53 mean_circ: 0.944022
Ran for 5550 frames Cell Events: 67 mean_circ: 0.941915
Ran for 6050 frames Cell Events: 73 mean_circ: 0.943235
Ran for 7550 frames Cell Events: 97 mean_circ: 0.94357
Ran for 8050 frames Cell Events: 110 mean_circ: 0.943213
Ran for 8550 frames Cell Events: 115 mean_circ: 0.94273
Ran for 9050 frames Cell Events: 122 mean_circ: 0.942318
Ran for 9550 frames Cell Events: 128 mean_circ: 0.94084
Ran for 10050 frames Cell Events: 137 mean_circ: 0.940196
Ran for 10550 frames Cell Events: 139 mean_circ: 0.940424
Ran for 11050 frames Cell Events: 142 mean_circ: 0.939567
Ran for 11550 frames Cell Events: 152 mean_circ: 0.939363
Ran for 12050 frames Cell Events: 161 mean_circ: 0.938086
Ran for 12550 frames Cell Events: 163 mean_circ: 0.937853
Ran for 13050 frames Cell Events: 172 mean_circ: 0.93703
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Ran for 15550 frames Cell Events: 187 mean_circ: 0.937397
Ran for 16050 frames Cell Events: 190 mean_circ: 0.937689
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Ran for 17550 frames Cell Events: 213 mean_circ: 0.938979
Ran for 19050 frames Cell Events: 239 mean_circ: 0.939154
Ran for 19550 frames Cell Events: 247 mean_circ: 0.937984
Ran for 20050 frames Cell Events: 259 mean_circ: 0.938069
Ran for 20550 frames Cell Events: 271 mean_circ: 0.938246
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Ran for 22550 frames Cell Events: 294 mean_circ: 0.93772
Ran for 23550 frames Cell Events: 308 mean_circ: 0.938154
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Ran for 24550 frames Cell Events: 326 mean_circ: 0.938349
Ran for 25550 frames Cell Events: 342 mean_circ: 0.937631
Ran for 26050 frames Cell Events: 352 mean_circ: 0.937552
Ran for 27050 frames Cell Events: 373 mean_circ: 0.938029
Ran for 27550 frames Cell Events: 376 mean_circ: 0.936917
Ran for 28050 frames Cell Events: 382 mean_circ: 0.937154
Ran for 28550 frames Cell Events: 389 mean_circ: 0.937134
Ran for 29550 frames Cell Events: 395 mean_circ: 0.937306
Ran for 30050 frames Cell Events: 400 mean_circ: 0.937071
Ran for 30550 frames Cell Events: 409 mean_circ: 0.937165
Ran for 31050 frames Cell Events: 414 mean_circ: 0.937435
Ran for 31550 frames Cell Events: 423 mean_circ: 0.937627
Ran for 32050 frames Cell Events: 432 mean_circ: 0.937798
Ran for 32550 frames Cell Events: 440 mean_circ: 0.938104
Ran for 33050 frames Cell Events: 450 mean_circ: 0.93731
Ran for 33550 frames Cell Events: 461 mean_circ: 0.937311
Ran for 34050 frames Cell Events: 469 mean_circ: 0.936859
Ran for 34550 frames Cell Events: 476 mean_circ: 0.936904
Ran for 35050 frames Cell Events: 481 mean_circ: 0.936909
Ran for 35550 frames Cell Events: 487 mean_circ: 0.937284
Ran for 36050 frames Cell Events: 496 mean_circ: 0.937473
Ran for 36550 frames Cell Events: 501 mean_circ: 0.937991
Ran for 37050 frames Cell Events: 506 mean_circ: 0.938099
Ran for 37550 frames Cell Events: 517 mean_circ: 0.938402
Ran for 38050 frames Cell Events: 525 mean_circ: 0.938549
Ran for 38550 frames Cell Events: 533 mean_circ: 0.938799
Ran for 39050 frames Cell Events: 547 mean_circ: 0.939347
Ran for 39550 frames Cell Events: 560 mean_circ: 0.939467
Ran for 40550 frames Cell Events: 583 mean_circ: 0.939736
Ran for 41050 frames Cell Events: 600 mean_circ: 0.939916
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Ran for 42550 frames Cell Events: 626 mean_circ: 0.939691
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Ran for 51550 frames Cell Events: 709 mean_circ: 0.939689
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Ran for 53550 frames Cell Events: 731 mean_circ: 0.938627
Ran for 54050 frames Cell Events: 735 mean_circ: 0.938668
Ran for 54550 frames Cell Events: 744 mean_circ: 0.938821
Ran for 55050 frames Cell Events: 748 mean_circ: 0.938881
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Ran for 63550 frames Cell Events: 868 mean_circ: 0.938771
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Ran for 64550 frames Cell Events: 875 mean_circ: 0.938695
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Ran for 66550 frames Cell Events: 890 mean_circ: 0.93869
Ran for 67050 frames Cell Events: 895 mean_circ: 0.938701
Ran for 67550 frames Cell Events: 902 mean_circ: 0.93854
Ran for 68050 frames Cell Events: 905 mean_circ: 0.938624
Ran for 69050 frames Cell Events: 915 mean_circ: 0.938643
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Ran for 70550 frames Cell Events: 926 mean_circ: 0.938597
Ran for 71050 frames Cell Events: 932 mean_circ: 0.938722
Ran for 71550 frames Cell Events: 933 mean_circ: 0.938714
Ran for 72050 frames Cell Events: 933 mean_circ: 0.938714
Ran for 72550 frames Cell Events: 938 mean_circ: 0.938728
Ran for 73050 frames Cell Events: 949 mean_circ: 0.938934
Ran for 73550 frames Cell Events: 955 mean_circ: 0.938966
Ran for 74050 frames Cell Events: 960 mean_circ: 0.939117
Ran for 74550 frames Cell Events: 963 mean_circ: 0.938996
Ran for 75050 frames Cell Events: 969 mean_circ: 0.938871
Ran for 75550 frames Cell Events: 975 mean_circ: 0.938917
Ran for 76050 frames Cell Events: 976 mean_circ: 0.938944
Ran for 76550 frames Cell Events: 977 mean_circ: 0.93895
Ran for 77050 frames Cell Events: 984 mean_circ: 0.938902
Ran for 77550 frames Cell Events: 989 mean_circ: 0.93897
Ran for 78050 frames Cell Events: 998 mean_circ: 0.938791
Ran for 78550 frames Cell Events: 1008 mean_circ: 0.938698
Ran for 79550 frames Cell Events: 1031 mean_circ: 0.938428
Ran for 80050 frames Cell Events: 1037 mean_circ: 0.93828
Ran for 80550 frames Cell Events: 1044 mean_circ: 0.938198
Ran for 81050 frames Cell Events: 1053 mean_circ: 0.938132
Ran for 81550 frames Cell Events: 1058 mean_circ: 0.938153
Ran for 82550 frames Cell Events: 1069 mean_circ: 0.938147
Ran for 83050 frames Cell Events: 1074 mean_circ: 0.937989
Ran for 83550 frames Cell Events: 1080 mean_circ: 0.938084
Ran for 84050 frames Cell Events: 1088 mean_circ: 0.938177
Ran for 84550 frames Cell Events: 1092 mean_circ: 0.938201
Ran for 85550 frames Cell Events: 1106 mean_circ: 0.938323
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Ran for 86550 frames Cell Events: 1118 mean_circ: 0.9382
Ran for 87050 frames Cell Events: 1127 mean_circ: 0.938215
Ran for 87550 frames Cell Events: 1134 mean_circ: 0.938222
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Ran for 91050 frames Cell Events: 1174 mean_circ: 0.938401
Ran for 92050 frames Cell Events: 1183 mean_circ: 0.938339
Ran for 92550 frames Cell Events: 1191 mean_circ: 0.938397
Ran for 93050 frames Cell Events: 1199 mean_circ: 0.938437
Ran for 93550 frames Cell Events: 1212 mean_circ: 0.938537
Ran for 94050 frames Cell Events: 1219 mean_circ: 0.938532
Ran for 94550 frames Cell Events: 1222 mean_circ: 0.938444
Ran for 95050 frames Cell Events: 1229 mean_circ: 0.938435
Ran for 95550 frames Cell Events: 1234 mean_circ: 0.938442
Ran for 97050 frames Cell Events: 1261 mean_circ: 0.938342
Ran for 97550 frames Cell Events: 1272 mean_circ: 0.938529
Ran for 98050 frames Cell Events: 1281 mean_circ: 0.938606
Ran for 98550 frames Cell Events: 1287 mean_circ: 0.93882
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Ran for 100050 frames Cell Events: 1310 mean_circ: 0.938803
Ran for 101050 frames Cell Events: 1321 mean_circ: 0.938651
Ran for 101550 frames Cell Events: 1331 mean_circ: 0.938319
Ran for 102050 frames Cell Events: 1337 mean_circ: 0.938176
Ran for 103550 frames Cell Events: 1358 mean_circ: 0.938422
Ran for 104550 frames Cell Events: 1375 mean_circ: 0.938615
Ran for 105550 frames Cell Events: 1386 mean_circ: 0.938741
Ran for 106050 frames Cell Events: 1391 mean_circ: 0.938724
Ran for 106550 frames Cell Events: 1398 mean_circ: 0.938472
Ran for 107050 frames Cell Events: 1403 mean_circ: 0.938571
Ran for 107550 frames Cell Events: 1409 mean_circ: 0.93859
Ran for 108050 frames Cell Events: 1412 mean_circ: 0.938617
Ran for 108550 frames Cell Events: 1413 mean_circ: 0.938624
Ran for 109050 frames Cell Events: 1421 mean_circ: 0.938489
Ran for 109550 frames Cell Events: 1431 mean_circ: 0.938083
Ran for 110050 frames Cell Events: 1437 mean_circ: 0.938031
Ran for 110550 frames Cell Events: 1445 mean_circ: 0.93805
Ran for 111050 frames Cell Events: 1447 mean_circ: 0.938113
Ran for 112050 frames Cell Events: 1460 mean_circ: 0.938044
Ran for 112550 frames Cell Events: 1465 mean_circ: 0.93799
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Ran for 116550 frames Cell Events: 1538 mean_circ: 0.937941
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Ran for 120050 frames Cell Events: 1601 mean_circ: 0.938295
Ran for 121050 frames Cell Events: 1611 mean_circ: 0.938257
Ran for 121550 frames Cell Events: 1616 mean_circ: 0.938287
Ran for 122050 frames Cell Events: 1622 mean_circ: 0.938273
Ran for 122550 frames Cell Events: 1629 mean_circ: 0.938303
Ran for 123050 frames Cell Events: 1639 mean_circ: 0.93826
Ran for 123550 frames Cell Events: 1642 mean_circ: 0.938326
Ran for 124050 frames Cell Events: 1648 mean_circ: 0.93833
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Ran for 152050 frames Cell Events: 2008 mean_circ: 0.938107
Ran for 152550 frames Cell Events: 2014 mean_circ: 0.938136
Ran for 153050 frames Cell Events: 2021 mean_circ: 0.938082
Ran for 153550 frames Cell Events: 2029 mean_circ: 0.937991
Ran for 154050 frames Cell Events: 2038 mean_circ: 0.937975
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Ran for 157050 frames Cell Events: 2083 mean_circ: 0.938044
Ran for 157550 frames Cell Events: 2085 mean_circ: 0.938083
Ran for 158050 frames Cell Events: 2088 mean_circ: 0.93811
Ran for 158550 frames Cell Events: 2092 mean_circ: 0.93809
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Ran for 575050 frames Cell Events: 7953 mean_circ: 0.934034
Ran for 575550 frames Cell Events: 7960 mean_circ: 0.934053
Ran for 576050 frames Cell Events: 7968 mean_circ: 0.934036
Ran for 576550 frames Cell Events: 7976 mean_circ: 0.934031
Ran for 577050 frames Cell Events: 7988 mean_circ: 0.934049
Ran for 577550 frames Cell Events: 7998 mean_circ: 0.934041
Ran for 578050 frames Cell Events: 8005 mean_circ: 0.934045
Ran for 579050 frames Cell Events: 8016 mean_circ: 0.93405
Ran for 579550 frames Cell Events: 8021 mean_circ: 0.934055
Ran for 580050 frames Cell Events: 8027 mean_circ: 0.934022
Ran for 580550 frames Cell Events: 8033 mean_circ: 0.93402
Ran for 581050 frames Cell Events: 8042 mean_circ: 0.93401
Ran for 581550 frames Cell Events: 8048 mean_circ: 0.934019
Ran for 582050 frames Cell Events: 8057 mean_circ: 0.934019
Ran for 582550 frames Cell Events: 8066 mean_circ: 0.934038
Ran for 583050 frames Cell Events: 8072 mean_circ: 0.934029
Ran for 584050 frames Cell Events: 8088 mean_circ: 0.934044
Ran for 584550 frames Cell Events: 8100 mean_circ: 0.934051
Ran for 585050 frames Cell Events: 8109 mean_circ: 0.933999
Ran for 585550 frames Cell Events: 8119 mean_circ: 0.933983
Ran for 586050 frames Cell Events: 8125 mean_circ: 0.933998
Ran for 586550 frames Cell Events: 8129 mean_circ: 0.933988
Ran for 587550 frames Cell Events: 8141 mean_circ: 0.934023
Ran for 588050 frames Cell Events: 8150 mean_circ: 0.934027
Ran for 588550 frames Cell Events: 8156 mean_circ: 0.934022
Ran for 589050 frames Cell Events: 8161 mean_circ: 0.934018
Ran for 589550 frames Cell Events: 8168 mean_circ: 0.934023
Ran for 590050 frames Cell Events: 8171 mean_circ: 0.933999
Ran for 591050 frames Cell Events: 8184 mean_circ: 0.93403
Ran for 591550 frames Cell Events: 8189 mean_circ: 0.93404
Ran for 592050 frames Cell Events: 8195 mean_circ: 0.934013
Ran for 592550 frames Cell Events: 8200 mean_circ: 0.934026
Ran for 593550 frames Cell Events: 8216 mean_circ: 0.934003
Ran for 594050 frames Cell Events: 8221 mean_circ: 0.933991
Ran for 595050 frames Cell Events: 8238 mean_circ: 0.933994
Ran for 596050 frames Cell Events: 8251 mean_circ: 0.933983
Ran for 596550 frames Cell Events: 8257 mean_circ: 0.933993
Ran for 597050 frames Cell Events: 8261 mean_circ: 0.933968
Ran for 598050 frames Cell Events: 8279 mean_circ: 0.933943
Ran for 598550 frames Cell Events: 8287 mean_circ: 0.933963
Ran for 599550 frames Cell Events: 8307 mean_circ: 0.934002
Ran for 600050 frames Cell Events: 8312 mean_circ: 0.933985
Ran for 601050 frames Cell Events: 8325 mean_circ: 0.934
Ran for 601550 frames Cell Events: 8331 mean_circ: 0.934017
Ran for 602050 frames Cell Events: 8337 mean_circ: 0.934034
Ran for 602550 frames Cell Events: 8340 mean_circ: 0.934045
Ran for 603050 frames Cell Events: 8344 mean_circ: 0.934036
Ran for 603550 frames Cell Events: 8354 mean_circ: 0.934042
Ran for 604550 frames Cell Events: 8369 mean_circ: 0.933986
Ran for 605050 frames Cell Events: 8376 mean_circ: 0.934004
Ran for 605550 frames Cell Events: 8385 mean_circ: 0.933968
Ran for 606050 frames Cell Events: 8387 mean_circ: 0.933976
Ran for 606550 frames Cell Events: 8391 mean_circ: 0.933976
Ran for 607050 frames Cell Events: 8394 mean_circ: 0.933982
Ran for 607550 frames Cell Events: 8397 mean_circ: 0.933957
Ran for 608550 frames Cell Events: 8417 mean_circ: 0.933964
Ran for 609050 frames Cell Events: 8424 mean_circ: 0.933955
Ran for 609550 frames Cell Events: 8429 mean_circ: 0.93396
Ran for 610050 frames Cell Events: 8436 mean_circ: 0.933973
Ran for 611050 frames Cell Events: 8451 mean_circ: 0.93397
Ran for 611550 frames Cell Events: 8455 mean_circ: 0.933968
Ran for 612050 frames Cell Events: 8461 mean_circ: 0.933962
Ran for 612550 frames Cell Events: 8466 mean_circ: 0.933972
Ran for 613050 frames Cell Events: 8477 mean_circ: 0.933996
Ran for 613550 frames Cell Events: 8485 mean_circ: 0.934
Ran for 614050 frames Cell Events: 8489 mean_circ: 0.933984
Ran for 614550 frames Cell Events: 8494 mean_circ: 0.933958
Ran for 615050 frames Cell Events: 8501 mean_circ: 0.933965
Ran for 615550 frames Cell Events: 8512 mean_circ: 0.933941
Ran for 616050 frames Cell Events: 8521 mean_circ: 0.933969
Ran for 616550 frames Cell Events: 8529 mean_circ: 0.933973
Ran for 617050 frames Cell Events: 8533 mean_circ: 0.933971
Ran for 617550 frames Cell Events: 8539 mean_circ: 0.933965
Ran for 618050 frames Cell Events: 8544 mean_circ: 0.933953
Ran for 619050 frames Cell Events: 8558 mean_circ: 0.933988
Ran for 619550 frames Cell Events: 8564 mean_circ: 0.933966
Ran for 620050 frames Cell Events: 8570 mean_circ: 0.933953
Ran for 620550 frames Cell Events: 8578 mean_circ: 0.933963
Ran for 621050 frames Cell Events: 8579 mean_circ: 0.933949
Ran for 622550 frames Cell Events: 8600 mean_circ: 0.933922
Ran for 623050 frames Cell Events: 8604 mean_circ: 0.933928
Ran for 623550 frames Cell Events: 8612 mean_circ: 0.933912
Ran for 624050 frames Cell Events: 8616 mean_circ: 0.933911
Ran for 624550 frames Cell Events: 8625 mean_circ: 0.933926
Ran for 625050 frames Cell Events: 8630 mean_circ: 0.93393
Ran for 626050 frames Cell Events: 8650 mean_circ: 0.933913
End this measurment..
Stopping time: 2.36236e+009
****************************************
Measurement finished
Mean Circularitiy: 0.933901
Mean Area: 711.332
Mean FL-1 intensity: 3173.94
Mean FL-2 intensity: 4021.33
Mean FL-3 intensity: 0
frames processed: 625966
Total Time: 313146ms
Number of Runs (new bg img) 0
Frames collected: 626133
Skipped frames: 39140
Rescued Frames: 3421232
Samples DAQ: 156534495
Samples written: 313066234
Samples per Channel: 156533117
FPS: 1999.49
Samples per second: 499873
Expected samples/s: 500000
Events: 8651
Events per second: 27.6261
jumped back (PMT): 7058
#Frames read, frequency
# 0 frames :805293
# 1 frames :415267
# 2 frames :8288
# 3 frames :4486
# 4 frames :2236
# 5 frames :1654
# 6 frames :1253
# 7 frames :2045
# 8 frames :1574
# 9 frames :1125
# 10 frames :2174
# 11 frames :1644
# 12 frames :1320
# 13 frames :831
# 14 frames :598
# 15 frames :513
# 16 frames :439
# 17 frames :336
# 18 frames :251
# 19 frames :239
# 20 frames :139
# 21 frames :119
# 22 frames :65
# 23 frames :60
# 24 frames :36
# 25 frames :27
# 26 frames :23
# 27 frames :25
# 28 frames :22
# 29 frames :12
# 30 frames :13
# 31 frames :11
# 32 frames :11
# 33 frames :6
# 34 frames :4
# 35 frames :7
# 36 frames :2
# 37 frames :3
# 38 frames :2
# 39 frames :2
# 40 frames :1
# 41 frames :4
# 42 frames :1
# 43 frames :2
# 44 frames :1
# 45 frames :1
# 47 frames :2
# 48 frames :3
# 49 frames :1
# 51 frames :1
# 54 frames :1
# 62 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.939
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 13e318aff965c2a644f6b017426a0a6c |
format | RT-FDC |
id | 6c3c9a40-f0b7-4b44-88ed-18e2f6c21950 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | 7494c462-bd84-42b1-a0c5-f5bc1357ec51 |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/6c3/c9a/40-f0b7-4b44-88ed-18e2f6c21950 |
sha256 | 23437022a81e9b79dabdc2f2cf90ffb39ae717c3e923554c3de3bb9896d7ccbc |
size | 151.1 MiB |
dc:experiment:date | 2017-06-11 |
dc:experiment:event count | 8,650 |
dc:experiment:run identifier | None-09ac |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170611_1630_dsRNA_N7_col |
dc:experiment:time | 16:30:46 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |