M1_20170611_1637_dsRNA_N7_faster.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.12.35
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5a69dde8caf5660ccaec77442bd8d898",
"name": "M1_20170611_1637_dsRNA_N7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:12:55"
}
}
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9448
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.36244e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.933915
Ran for 550 frames Cell Events: 6 mean_circ: 0.931124
Ran for 1050 frames Cell Events: 12 mean_circ: 0.933017
Ran for 1550 frames Cell Events: 18 mean_circ: 0.919981
Ran for 2050 frames Cell Events: 24 mean_circ: 0.915311
Ran for 3050 frames Cell Events: 37 mean_circ: 0.920832
Ran for 4050 frames Cell Events: 52 mean_circ: 0.916903
Ran for 4550 frames Cell Events: 65 mean_circ: 0.919995
Ran for 5050 frames Cell Events: 75 mean_circ: 0.918775
Ran for 6050 frames Cell Events: 88 mean_circ: 0.919216
Ran for 6550 frames Cell Events: 92 mean_circ: 0.918555
Ran for 7050 frames Cell Events: 103 mean_circ: 0.919151
Ran for 7550 frames Cell Events: 107 mean_circ: 0.916125
Ran for 8050 frames Cell Events: 111 mean_circ: 0.915674
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Ran for 10050 frames Cell Events: 144 mean_circ: 0.911007
Ran for 10550 frames Cell Events: 153 mean_circ: 0.910272
Ran for 11050 frames Cell Events: 159 mean_circ: 0.90912
Ran for 11550 frames Cell Events: 169 mean_circ: 0.908631
Ran for 12050 frames Cell Events: 173 mean_circ: 0.909318
Ran for 12550 frames Cell Events: 178 mean_circ: 0.909608
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Ran for 24050 frames Cell Events: 326 mean_circ: 0.913411
Ran for 24550 frames Cell Events: 332 mean_circ: 0.913151
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Ran for 25550 frames Cell Events: 342 mean_circ: 0.913698
Ran for 26050 frames Cell Events: 349 mean_circ: 0.913556
Ran for 27050 frames Cell Events: 362 mean_circ: 0.913912
Ran for 27550 frames Cell Events: 367 mean_circ: 0.913474
Ran for 28050 frames Cell Events: 374 mean_circ: 0.913714
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Ran for 31550 frames Cell Events: 418 mean_circ: 0.915171
Ran for 32050 frames Cell Events: 424 mean_circ: 0.915555
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Ran for 33550 frames Cell Events: 447 mean_circ: 0.916485
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Ran for 68550 frames Cell Events: 974 mean_circ: 0.919808
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Ran for 70550 frames Cell Events: 1002 mean_circ: 0.92
Ran for 71050 frames Cell Events: 1009 mean_circ: 0.920122
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Ran for 72050 frames Cell Events: 1019 mean_circ: 0.919991
Ran for 72550 frames Cell Events: 1027 mean_circ: 0.919706
Ran for 73050 frames Cell Events: 1037 mean_circ: 0.919924
Ran for 73550 frames Cell Events: 1044 mean_circ: 0.920089
Ran for 74050 frames Cell Events: 1055 mean_circ: 0.920203
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Ran for 75050 frames Cell Events: 1069 mean_circ: 0.920253
Ran for 75550 frames Cell Events: 1073 mean_circ: 0.920239
Ran for 76050 frames Cell Events: 1079 mean_circ: 0.920055
Ran for 77550 frames Cell Events: 1101 mean_circ: 0.920423
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Ran for 80050 frames Cell Events: 1132 mean_circ: 0.920497
Ran for 80550 frames Cell Events: 1141 mean_circ: 0.920443
Ran for 81050 frames Cell Events: 1148 mean_circ: 0.920243
Ran for 81550 frames Cell Events: 1155 mean_circ: 0.920191
Ran for 83050 frames Cell Events: 1188 mean_circ: 0.919784
Ran for 83550 frames Cell Events: 1201 mean_circ: 0.919727
Ran for 84050 frames Cell Events: 1206 mean_circ: 0.919602
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Ran for 372050 frames Cell Events: 5400 mean_circ: 0.920611
Ran for 372550 frames Cell Events: 5407 mean_circ: 0.920637
Ran for 373050 frames Cell Events: 5414 mean_circ: 0.920633
Ran for 373550 frames Cell Events: 5419 mean_circ: 0.920616
Ran for 374050 frames Cell Events: 5425 mean_circ: 0.92062
Ran for 374550 frames Cell Events: 5429 mean_circ: 0.920632
Ran for 375050 frames Cell Events: 5434 mean_circ: 0.920614
Ran for 375550 frames Cell Events: 5437 mean_circ: 0.92062
Ran for 376550 frames Cell Events: 5448 mean_circ: 0.92061
Ran for 377050 frames Cell Events: 5458 mean_circ: 0.92063
Ran for 377550 frames Cell Events: 5467 mean_circ: 0.920647
Ran for 378550 frames Cell Events: 5478 mean_circ: 0.920628
Ran for 379050 frames Cell Events: 5481 mean_circ: 0.92065
Ran for 379550 frames Cell Events: 5487 mean_circ: 0.920629
Ran for 380050 frames Cell Events: 5492 mean_circ: 0.920627
Ran for 380550 frames Cell Events: 5499 mean_circ: 0.920649
Ran for 381050 frames Cell Events: 5507 mean_circ: 0.920684
Ran for 381550 frames Cell Events: 5513 mean_circ: 0.920677
Ran for 382050 frames Cell Events: 5520 mean_circ: 0.920697
Ran for 382550 frames Cell Events: 5525 mean_circ: 0.920639
Ran for 383050 frames Cell Events: 5533 mean_circ: 0.920634
Ran for 383550 frames Cell Events: 5539 mean_circ: 0.920556
Ran for 384050 frames Cell Events: 5548 mean_circ: 0.920515
Ran for 384550 frames Cell Events: 5557 mean_circ: 0.92049
Ran for 385050 frames Cell Events: 5564 mean_circ: 0.920495
Ran for 385550 frames Cell Events: 5574 mean_circ: 0.920468
Ran for 386050 frames Cell Events: 5585 mean_circ: 0.92047
Ran for 386550 frames Cell Events: 5588 mean_circ: 0.920419
Ran for 387050 frames Cell Events: 5592 mean_circ: 0.920433
Ran for 387550 frames Cell Events: 5597 mean_circ: 0.920448
Ran for 388050 frames Cell Events: 5600 mean_circ: 0.920486
Ran for 388550 frames Cell Events: 5606 mean_circ: 0.920533
Ran for 389050 frames Cell Events: 5615 mean_circ: 0.920544
Ran for 389550 frames Cell Events: 5618 mean_circ: 0.920504
Ran for 390050 frames Cell Events: 5624 mean_circ: 0.92052
Ran for 391050 frames Cell Events: 5634 mean_circ: 0.920528
Ran for 391550 frames Cell Events: 5643 mean_circ: 0.92051
Ran for 392050 frames Cell Events: 5651 mean_circ: 0.920523
Ran for 392550 frames Cell Events: 5656 mean_circ: 0.920493
Ran for 393050 frames Cell Events: 5661 mean_circ: 0.920434
Ran for 393550 frames Cell Events: 5670 mean_circ: 0.920427
Ran for 394050 frames Cell Events: 5675 mean_circ: 0.920438
Ran for 395050 frames Cell Events: 5692 mean_circ: 0.920427
Ran for 395550 frames Cell Events: 5702 mean_circ: 0.920445
Ran for 396050 frames Cell Events: 5713 mean_circ: 0.920434
Ran for 397550 frames Cell Events: 5739 mean_circ: 0.920398
Ran for 398050 frames Cell Events: 5743 mean_circ: 0.92035
Ran for 398550 frames Cell Events: 5750 mean_circ: 0.920361
Ran for 399050 frames Cell Events: 5759 mean_circ: 0.920331
Ran for 399550 frames Cell Events: 5766 mean_circ: 0.920332
Ran for 400050 frames Cell Events: 5769 mean_circ: 0.920349
Ran for 401050 frames Cell Events: 5784 mean_circ: 0.92038
Ran for 401550 frames Cell Events: 5787 mean_circ: 0.920363
Ran for 402050 frames Cell Events: 5803 mean_circ: 0.920382
Ran for 402550 frames Cell Events: 5810 mean_circ: 0.920385
Ran for 403050 frames Cell Events: 5817 mean_circ: 0.920425
Ran for 403550 frames Cell Events: 5824 mean_circ: 0.920419
Ran for 404050 frames Cell Events: 5831 mean_circ: 0.920414
Ran for 405050 frames Cell Events: 5850 mean_circ: 0.920437
Ran for 405550 frames Cell Events: 5854 mean_circ: 0.92044
Ran for 406050 frames Cell Events: 5858 mean_circ: 0.920414
Ran for 406550 frames Cell Events: 5863 mean_circ: 0.920439
Ran for 407050 frames Cell Events: 5867 mean_circ: 0.920442
Ran for 407550 frames Cell Events: 5877 mean_circ: 0.920432
Ran for 408050 frames Cell Events: 5882 mean_circ: 0.920417
Ran for 408550 frames Cell Events: 5892 mean_circ: 0.920417
Ran for 409050 frames Cell Events: 5896 mean_circ: 0.920422
Ran for 409550 frames Cell Events: 5900 mean_circ: 0.920389
Ran for 410050 frames Cell Events: 5907 mean_circ: 0.920388
Ran for 411050 frames Cell Events: 5925 mean_circ: 0.92035
Ran for 411550 frames Cell Events: 5931 mean_circ: 0.920348
Ran for 412050 frames Cell Events: 5937 mean_circ: 0.920313
Ran for 412550 frames Cell Events: 5939 mean_circ: 0.920323
Ran for 413550 frames Cell Events: 5950 mean_circ: 0.920302
Ran for 414050 frames Cell Events: 5957 mean_circ: 0.920312
Ran for 414550 frames Cell Events: 5966 mean_circ: 0.920279
Ran for 415050 frames Cell Events: 5975 mean_circ: 0.920201
Ran for 415550 frames Cell Events: 5986 mean_circ: 0.920186
Ran for 416550 frames Cell Events: 5998 mean_circ: 0.920151
Ran for 417050 frames Cell Events: 6006 mean_circ: 0.920181
Ran for 417550 frames Cell Events: 6015 mean_circ: 0.920181
Ran for 418050 frames Cell Events: 6025 mean_circ: 0.920137
Ran for 418550 frames Cell Events: 6033 mean_circ: 0.920099
Ran for 419050 frames Cell Events: 6038 mean_circ: 0.920092
Ran for 419550 frames Cell Events: 6044 mean_circ: 0.920094
Ran for 420050 frames Cell Events: 6053 mean_circ: 0.92011
Ran for 420550 frames Cell Events: 6058 mean_circ: 0.920112
Ran for 421050 frames Cell Events: 6066 mean_circ: 0.920128
Ran for 421550 frames Cell Events: 6070 mean_circ: 0.920122
Ran for 422050 frames Cell Events: 6082 mean_circ: 0.920133
Ran for 422550 frames Cell Events: 6089 mean_circ: 0.920113
Ran for 423050 frames Cell Events: 6097 mean_circ: 0.920137
Ran for 423550 frames Cell Events: 6103 mean_circ: 0.920146
Ran for 424050 frames Cell Events: 6105 mean_circ: 0.920148
Ran for 424550 frames Cell Events: 6112 mean_circ: 0.920153
Ran for 425050 frames Cell Events: 6120 mean_circ: 0.920138
Ran for 426050 frames Cell Events: 6138 mean_circ: 0.920127
Ran for 427050 frames Cell Events: 6155 mean_circ: 0.920164
Ran for 427550 frames Cell Events: 6162 mean_circ: 0.920164
Ran for 428050 frames Cell Events: 6166 mean_circ: 0.920168
Ran for 428550 frames Cell Events: 6171 mean_circ: 0.920165
Ran for 429050 frames Cell Events: 6182 mean_circ: 0.920091
Ran for 429550 frames Cell Events: 6191 mean_circ: 0.920106
Ran for 430050 frames Cell Events: 6196 mean_circ: 0.920043
Ran for 430550 frames Cell Events: 6201 mean_circ: 0.920046
Ran for 431050 frames Cell Events: 6205 mean_circ: 0.920036
Ran for 431550 frames Cell Events: 6211 mean_circ: 0.920048
Ran for 432050 frames Cell Events: 6219 mean_circ: 0.920028
Ran for 432550 frames Cell Events: 6227 mean_circ: 0.920027
Ran for 433050 frames Cell Events: 6230 mean_circ: 0.92004
Ran for 433550 frames Cell Events: 6238 mean_circ: 0.920062
Ran for 434050 frames Cell Events: 6249 mean_circ: 0.920038
Ran for 434550 frames Cell Events: 6263 mean_circ: 0.920046
Ran for 435050 frames Cell Events: 6266 mean_circ: 0.920035
Ran for 435550 frames Cell Events: 6280 mean_circ: 0.920004
Ran for 437050 frames Cell Events: 6303 mean_circ: 0.920031
Ran for 437550 frames Cell Events: 6311 mean_circ: 0.920022
Ran for 438050 frames Cell Events: 6317 mean_circ: 0.920021
Ran for 438550 frames Cell Events: 6320 mean_circ: 0.920015
Ran for 439050 frames Cell Events: 6331 mean_circ: 0.919989
Ran for 439550 frames Cell Events: 6338 mean_circ: 0.919982
Ran for 440050 frames Cell Events: 6345 mean_circ: 0.919998
Ran for 440550 frames Cell Events: 6350 mean_circ: 0.919991
Ran for 441050 frames Cell Events: 6357 mean_circ: 0.919966
Ran for 441550 frames Cell Events: 6363 mean_circ: 0.919978
Ran for 442050 frames Cell Events: 6370 mean_circ: 0.919907
Ran for 442550 frames Cell Events: 6382 mean_circ: 0.919899
Ran for 443050 frames Cell Events: 6390 mean_circ: 0.919932
Ran for 443550 frames Cell Events: 6399 mean_circ: 0.919933
Ran for 444050 frames Cell Events: 6406 mean_circ: 0.919909
Ran for 444550 frames Cell Events: 6415 mean_circ: 0.919892
Ran for 445050 frames Cell Events: 6422 mean_circ: 0.919903
Ran for 445550 frames Cell Events: 6426 mean_circ: 0.919909
Ran for 446050 frames Cell Events: 6432 mean_circ: 0.919879
Ran for 446550 frames Cell Events: 6440 mean_circ: 0.919898
Ran for 447050 frames Cell Events: 6445 mean_circ: 0.919862
Ran for 447550 frames Cell Events: 6449 mean_circ: 0.919884
Ran for 448050 frames Cell Events: 6456 mean_circ: 0.919918
Ran for 448550 frames Cell Events: 6465 mean_circ: 0.919933
Ran for 449050 frames Cell Events: 6471 mean_circ: 0.919869
Ran for 449550 frames Cell Events: 6480 mean_circ: 0.919856
Ran for 450050 frames Cell Events: 6485 mean_circ: 0.919856
Ran for 450550 frames Cell Events: 6493 mean_circ: 0.919853
Ran for 451050 frames Cell Events: 6503 mean_circ: 0.919851
Ran for 451550 frames Cell Events: 6512 mean_circ: 0.919863
Ran for 452050 frames Cell Events: 6520 mean_circ: 0.919857
Ran for 452550 frames Cell Events: 6530 mean_circ: 0.919855
Ran for 453050 frames Cell Events: 6539 mean_circ: 0.919848
Ran for 453550 frames Cell Events: 6550 mean_circ: 0.91984
Ran for 454050 frames Cell Events: 6561 mean_circ: 0.919882
Ran for 454550 frames Cell Events: 6569 mean_circ: 0.919921
Ran for 455050 frames Cell Events: 6577 mean_circ: 0.919912
Ran for 455550 frames Cell Events: 6582 mean_circ: 0.919908
Ran for 456050 frames Cell Events: 6585 mean_circ: 0.919908
Ran for 456550 frames Cell Events: 6590 mean_circ: 0.919907
Ran for 457050 frames Cell Events: 6599 mean_circ: 0.919915
Ran for 457550 frames Cell Events: 6610 mean_circ: 0.919902
Ran for 458050 frames Cell Events: 6619 mean_circ: 0.919921
Ran for 458550 frames Cell Events: 6623 mean_circ: 0.919947
Ran for 459050 frames Cell Events: 6631 mean_circ: 0.919947
Ran for 459550 frames Cell Events: 6641 mean_circ: 0.919968
Ran for 460050 frames Cell Events: 6647 mean_circ: 0.919961
Ran for 461050 frames Cell Events: 6672 mean_circ: 0.919987
Ran for 462050 frames Cell Events: 6694 mean_circ: 0.920026
Ran for 462550 frames Cell Events: 6701 mean_circ: 0.920025
Ran for 463050 frames Cell Events: 6706 mean_circ: 0.920039
Ran for 463550 frames Cell Events: 6718 mean_circ: 0.919974
Ran for 464050 frames Cell Events: 6732 mean_circ: 0.920007
Ran for 464550 frames Cell Events: 6737 mean_circ: 0.920027
Ran for 465050 frames Cell Events: 6744 mean_circ: 0.920027
Ran for 466050 frames Cell Events: 6755 mean_circ: 0.920047
Ran for 466550 frames Cell Events: 6762 mean_circ: 0.920054
Ran for 467050 frames Cell Events: 6775 mean_circ: 0.920076
Ran for 467550 frames Cell Events: 6787 mean_circ: 0.920069
Ran for 468550 frames Cell Events: 6803 mean_circ: 0.920072
Ran for 469050 frames Cell Events: 6807 mean_circ: 0.920082
Ran for 469550 frames Cell Events: 6815 mean_circ: 0.920098
Ran for 470050 frames Cell Events: 6820 mean_circ: 0.920101
Ran for 470550 frames Cell Events: 6827 mean_circ: 0.920081
Ran for 471050 frames Cell Events: 6830 mean_circ: 0.920083
Ran for 471550 frames Cell Events: 6839 mean_circ: 0.920105
Ran for 472050 frames Cell Events: 6844 mean_circ: 0.9201
Ran for 472550 frames Cell Events: 6853 mean_circ: 0.920102
Ran for 473050 frames Cell Events: 6860 mean_circ: 0.920082
Ran for 473550 frames Cell Events: 6865 mean_circ: 0.920085
Ran for 474050 frames Cell Events: 6871 mean_circ: 0.9201
Ran for 475050 frames Cell Events: 6892 mean_circ: 0.920129
Ran for 476050 frames Cell Events: 6906 mean_circ: 0.920077
Ran for 476550 frames Cell Events: 6914 mean_circ: 0.920092
Ran for 477050 frames Cell Events: 6921 mean_circ: 0.92011
Ran for 477550 frames Cell Events: 6923 mean_circ: 0.920121
Ran for 478550 frames Cell Events: 6944 mean_circ: 0.920113
Ran for 479050 frames Cell Events: 6947 mean_circ: 0.920091
Ran for 479550 frames Cell Events: 6954 mean_circ: 0.920078
Ran for 480050 frames Cell Events: 6959 mean_circ: 0.920071
Ran for 480550 frames Cell Events: 6967 mean_circ: 0.920029
Ran for 481050 frames Cell Events: 6974 mean_circ: 0.920013
Ran for 481550 frames Cell Events: 6979 mean_circ: 0.920003
Ran for 482050 frames Cell Events: 6986 mean_circ: 0.920004
Ran for 482550 frames Cell Events: 6994 mean_circ: 0.919994
Ran for 483050 frames Cell Events: 7000 mean_circ: 0.919975
Ran for 483550 frames Cell Events: 7005 mean_circ: 0.919955
Ran for 484550 frames Cell Events: 7018 mean_circ: 0.919909
Ran for 485050 frames Cell Events: 7027 mean_circ: 0.919908
Ran for 485550 frames Cell Events: 7031 mean_circ: 0.919898
Ran for 486050 frames Cell Events: 7042 mean_circ: 0.919886
Ran for 486550 frames Cell Events: 7047 mean_circ: 0.91985
Ran for 487050 frames Cell Events: 7057 mean_circ: 0.919863
Ran for 487550 frames Cell Events: 7070 mean_circ: 0.91986
Ran for 488050 frames Cell Events: 7079 mean_circ: 0.919847
Ran for 488550 frames Cell Events: 7083 mean_circ: 0.91986
Ran for 489050 frames Cell Events: 7090 mean_circ: 0.919816
Ran for 489550 frames Cell Events: 7096 mean_circ: 0.91979
Ran for 490050 frames Cell Events: 7101 mean_circ: 0.91978
Ran for 490550 frames Cell Events: 7104 mean_circ: 0.919789
Ran for 491550 frames Cell Events: 7118 mean_circ: 0.919778
Ran for 492550 frames Cell Events: 7127 mean_circ: 0.919762
Ran for 493050 frames Cell Events: 7135 mean_circ: 0.919783
Ran for 493550 frames Cell Events: 7144 mean_circ: 0.919751
Ran for 494050 frames Cell Events: 7151 mean_circ: 0.919728
Ran for 495550 frames Cell Events: 7167 mean_circ: 0.919708
Ran for 496550 frames Cell Events: 7177 mean_circ: 0.919723
Ran for 497050 frames Cell Events: 7191 mean_circ: 0.919747
Ran for 497550 frames Cell Events: 7203 mean_circ: 0.919699
Ran for 498050 frames Cell Events: 7207 mean_circ: 0.919659
Ran for 498550 frames Cell Events: 7218 mean_circ: 0.919654
Ran for 499050 frames Cell Events: 7227 mean_circ: 0.919646
Ran for 499550 frames Cell Events: 7237 mean_circ: 0.919658
Ran for 500050 frames Cell Events: 7247 mean_circ: 0.919659
Ran for 501050 frames Cell Events: 7268 mean_circ: 0.919618
Ran for 502050 frames Cell Events: 7285 mean_circ: 0.919644
Ran for 502550 frames Cell Events: 7291 mean_circ: 0.919651
Ran for 503050 frames Cell Events: 7298 mean_circ: 0.919635
Ran for 503550 frames Cell Events: 7306 mean_circ: 0.919616
Ran for 504050 frames Cell Events: 7309 mean_circ: 0.919635
Ran for 504550 frames Cell Events: 7314 mean_circ: 0.919641
Ran for 505050 frames Cell Events: 7321 mean_circ: 0.919638
Ran for 506050 frames Cell Events: 7335 mean_circ: 0.919665
Ran for 506550 frames Cell Events: 7339 mean_circ: 0.919684
Ran for 507050 frames Cell Events: 7346 mean_circ: 0.919673
Ran for 507550 frames Cell Events: 7353 mean_circ: 0.919663
Ran for 508050 frames Cell Events: 7363 mean_circ: 0.91968
Ran for 508550 frames Cell Events: 7371 mean_circ: 0.919679
Ran for 509050 frames Cell Events: 7378 mean_circ: 0.919687
Ran for 510050 frames Cell Events: 7389 mean_circ: 0.9197
Ran for 510550 frames Cell Events: 7398 mean_circ: 0.919719
Ran for 511050 frames Cell Events: 7405 mean_circ: 0.919723
Ran for 511550 frames Cell Events: 7413 mean_circ: 0.919751
Ran for 512050 frames Cell Events: 7416 mean_circ: 0.919761
Ran for 512550 frames Cell Events: 7422 mean_circ: 0.919771
Ran for 513050 frames Cell Events: 7431 mean_circ: 0.919724
Ran for 513550 frames Cell Events: 7438 mean_circ: 0.919719
Ran for 514050 frames Cell Events: 7443 mean_circ: 0.919709
Ran for 514550 frames Cell Events: 7454 mean_circ: 0.919726
Ran for 516050 frames Cell Events: 7486 mean_circ: 0.919713
Ran for 516550 frames Cell Events: 7499 mean_circ: 0.91972
Ran for 517050 frames Cell Events: 7508 mean_circ: 0.919744
Ran for 517550 frames Cell Events: 7516 mean_circ: 0.919707
Ran for 518050 frames Cell Events: 7519 mean_circ: 0.919706
Ran for 520050 frames Cell Events: 7545 mean_circ: 0.919613
Ran for 520550 frames Cell Events: 7555 mean_circ: 0.919549
Ran for 521050 frames Cell Events: 7559 mean_circ: 0.919565
Ran for 522050 frames Cell Events: 7581 mean_circ: 0.919538
Ran for 522550 frames Cell Events: 7587 mean_circ: 0.919536
Ran for 523050 frames Cell Events: 7598 mean_circ: 0.919518
Ran for 524050 frames Cell Events: 7607 mean_circ: 0.919485
Ran for 524550 frames Cell Events: 7612 mean_circ: 0.919494
Ran for 525550 frames Cell Events: 7625 mean_circ: 0.919485
Ran for 526050 frames Cell Events: 7631 mean_circ: 0.919466
Ran for 526550 frames Cell Events: 7640 mean_circ: 0.91947
Ran for 527050 frames Cell Events: 7653 mean_circ: 0.919432
Ran for 527550 frames Cell Events: 7663 mean_circ: 0.919411
Ran for 528050 frames Cell Events: 7668 mean_circ: 0.91942
Ran for 529050 frames Cell Events: 7680 mean_circ: 0.919421
Ran for 530050 frames Cell Events: 7699 mean_circ: 0.919389
Ran for 530550 frames Cell Events: 7708 mean_circ: 0.919345
Ran for 531050 frames Cell Events: 7713 mean_circ: 0.91935
Ran for 531550 frames Cell Events: 7717 mean_circ: 0.919373
Ran for 532550 frames Cell Events: 7728 mean_circ: 0.919327
Ran for 533050 frames Cell Events: 7733 mean_circ: 0.919345
Ran for 533550 frames Cell Events: 7741 mean_circ: 0.919343
Ran for 534050 frames Cell Events: 7743 mean_circ: 0.91932
Ran for 534550 frames Cell Events: 7748 mean_circ: 0.919322
Ran for 535050 frames Cell Events: 7755 mean_circ: 0.919314
Ran for 535550 frames Cell Events: 7767 mean_circ: 0.919308
Ran for 536050 frames Cell Events: 7773 mean_circ: 0.919341
Ran for 536550 frames Cell Events: 7785 mean_circ: 0.919349
Ran for 537550 frames Cell Events: 7802 mean_circ: 0.919336
Ran for 538050 frames Cell Events: 7807 mean_circ: 0.91933
Ran for 538550 frames Cell Events: 7813 mean_circ: 0.919333
Ran for 539050 frames Cell Events: 7821 mean_circ: 0.919375
Ran for 539550 frames Cell Events: 7832 mean_circ: 0.919402
Ran for 540050 frames Cell Events: 7839 mean_circ: 0.91938
Ran for 540550 frames Cell Events: 7843 mean_circ: 0.919376
Ran for 541050 frames Cell Events: 7851 mean_circ: 0.919379
Ran for 541550 frames Cell Events: 7853 mean_circ: 0.919381
Ran for 542050 frames Cell Events: 7863 mean_circ: 0.919408
Ran for 542550 frames Cell Events: 7871 mean_circ: 0.919397
Ran for 543050 frames Cell Events: 7876 mean_circ: 0.919413
Ran for 544050 frames Cell Events: 7888 mean_circ: 0.919429
Ran for 544550 frames Cell Events: 7895 mean_circ: 0.919424
Ran for 545050 frames Cell Events: 7901 mean_circ: 0.919423
Ran for 545550 frames Cell Events: 7907 mean_circ: 0.919424
Ran for 546050 frames Cell Events: 7912 mean_circ: 0.919432
Ran for 546550 frames Cell Events: 7922 mean_circ: 0.919435
Ran for 547050 frames Cell Events: 7925 mean_circ: 0.919438
Ran for 547550 frames Cell Events: 7928 mean_circ: 0.919439
Ran for 548050 frames Cell Events: 7936 mean_circ: 0.919462
Ran for 548550 frames Cell Events: 7942 mean_circ: 0.919449
Ran for 549050 frames Cell Events: 7945 mean_circ: 0.919424
Ran for 550050 frames Cell Events: 7956 mean_circ: 0.919423
Ran for 552050 frames Cell Events: 7980 mean_circ: 0.91939
Ran for 552550 frames Cell Events: 7991 mean_circ: 0.919401
Ran for 554050 frames Cell Events: 8008 mean_circ: 0.919413
Ran for 554550 frames Cell Events: 8010 mean_circ: 0.919414
Ran for 555050 frames Cell Events: 8020 mean_circ: 0.919406
Ran for 555550 frames Cell Events: 8023 mean_circ: 0.919411
End this measurment..
Stopping time: 2.36271e+009
****************************************
Measurement finished
Mean Circularitiy: 0.919415
Mean Area: 721.372
Mean FL-1 intensity: 3055.84
Mean FL-2 intensity: 4380.66
Mean FL-3 intensity: 0
frames processed: 551683
Total Time: 277905ms
Number of Runs (new bg img) 0
Frames collected: 555623
Skipped frames: 43080
Rescued Frames: 3567547
Samples DAQ: 138905818
Samples written: 277811282
Samples per Channel: 138905641
FPS: 1999.33
Samples per second: 499832
Expected samples/s: 500000
Events: 8024
Events per second: 28.8732
jumped back (PMT): 12
#Frames read, frequency
# 0 frames :705834
# 1 frames :377805
# 2 frames :7782
# 3 frames :3347
# 4 frames :1776
# 5 frames :1176
# 6 frames :1929
# 7 frames :2096
# 8 frames :1775
# 9 frames :1753
# 10 frames :1376
# 11 frames :900
# 12 frames :891
# 13 frames :581
# 14 frames :496
# 15 frames :380
# 16 frames :350
# 17 frames :301
# 18 frames :192
# 19 frames :142
# 20 frames :99
# 21 frames :44
# 22 frames :34
# 23 frames :22
# 24 frames :22
# 25 frames :18
# 26 frames :7
# 27 frames :8
# 28 frames :11
# 29 frames :12
# 30 frames :10
# 31 frames :5
# 32 frames :6
# 33 frames :5
# 34 frames :3
# 35 frames :2
# 36 frames :2
# 37 frames :1
# 38 frames :4
# 39 frames :1
# 41 frames :1
# 42 frames :1
# 43 frames :1
# 46 frames :1
# 53 frames :1
# 62 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9448
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 138dfe69bb0b7e2f1931dbfc1cd0c69f |
format | RT-FDC |
id | 2880e394-cbc0-4e04-8d22-84cac921652f |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | a7441914-8eb4-4d60-af87-1e867d872ead |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/288/0e3/94-cbc0-4e04-8d22-84cac921652f |
sha256 | 6bdef8489deb4f5be43ef247012114fbe2d245dbeb4e7d7c167abab784cb46c0 |
size | 137.5 MiB |
dc:experiment:date | 2017-06-11 |
dc:experiment:event count | 8,023 |
dc:experiment:run identifier | None-7034 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170611_1637_dsRNA_N7_faster |
dc:experiment:time | 16:37:11 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 200 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.06 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |