Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.22.20
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8fafb41450b158434fac5a71d31d97cd",
"name": "M1_20170611_1847_dsRNA_P2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:22:40"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8101 (max 8102) in '8101'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2223
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.37026e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 13 mean_circ: 0.91234
Ran for 1050 frames Cell Events: 35 mean_circ: 0.924377
Ran for 1550 frames Cell Events: 55 mean_circ: 0.931898
Ran for 2050 frames Cell Events: 63 mean_circ: 0.928033
Ran for 4050 frames Cell Events: 115 mean_circ: 0.929542
Ran for 5050 frames Cell Events: 150 mean_circ: 0.929019
Ran for 5550 frames Cell Events: 164 mean_circ: 0.93066
Ran for 6050 frames Cell Events: 178 mean_circ: 0.93097
Ran for 6550 frames Cell Events: 192 mean_circ: 0.931282
Ran for 7050 frames Cell Events: 210 mean_circ: 0.931792
Ran for 8550 frames Cell Events: 266 mean_circ: 0.932231
Ran for 9050 frames Cell Events: 285 mean_circ: 0.932756
Ran for 10050 frames Cell Events: 321 mean_circ: 0.933504
Ran for 11050 frames Cell Events: 359 mean_circ: 0.933905
Ran for 11550 frames Cell Events: 373 mean_circ: 0.934277
Ran for 12550 frames Cell Events: 394 mean_circ: 0.933737
Ran for 13050 frames Cell Events: 409 mean_circ: 0.933229
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Ran for 16050 frames Cell Events: 499 mean_circ: 0.934064
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Ran for 19050 frames Cell Events: 614 mean_circ: 0.934114
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Ran for 75550 frames Cell Events: 2554 mean_circ: 0.932962
Ran for 76550 frames Cell Events: 2602 mean_circ: 0.932804
Ran for 77550 frames Cell Events: 2635 mean_circ: 0.93291
Ran for 78550 frames Cell Events: 2658 mean_circ: 0.93282
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Ran for 82050 frames Cell Events: 2773 mean_circ: 0.932835
Ran for 82550 frames Cell Events: 2792 mean_circ: 0.932857
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Ran for 84050 frames Cell Events: 2849 mean_circ: 0.932602
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Ran for 85050 frames Cell Events: 2882 mean_circ: 0.932724
Ran for 86050 frames Cell Events: 2927 mean_circ: 0.932823
Ran for 86550 frames Cell Events: 2948 mean_circ: 0.932787
Ran for 88050 frames Cell Events: 2991 mean_circ: 0.932712
Ran for 89050 frames Cell Events: 3020 mean_circ: 0.932613
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Ran for 91550 frames Cell Events: 3099 mean_circ: 0.932612
Ran for 92050 frames Cell Events: 3114 mean_circ: 0.932606
Ran for 93050 frames Cell Events: 3173 mean_circ: 0.932625
Ran for 93550 frames Cell Events: 3191 mean_circ: 0.932614
Ran for 94050 frames Cell Events: 3214 mean_circ: 0.932538
Ran for 94550 frames Cell Events: 3234 mean_circ: 0.932444
Ran for 96550 frames Cell Events: 3295 mean_circ: 0.932499
Ran for 98050 frames Cell Events: 3347 mean_circ: 0.932632
Ran for 98550 frames Cell Events: 3367 mean_circ: 0.932697
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Ran for 99550 frames Cell Events: 3398 mean_circ: 0.932722
Ran for 100550 frames Cell Events: 3427 mean_circ: 0.93268
Ran for 103550 frames Cell Events: 3522 mean_circ: 0.932712
Ran for 104550 frames Cell Events: 3567 mean_circ: 0.93269
Ran for 105050 frames Cell Events: 3584 mean_circ: 0.93273
Ran for 105550 frames Cell Events: 3600 mean_circ: 0.93274
Ran for 106050 frames Cell Events: 3613 mean_circ: 0.932679
Ran for 106550 frames Cell Events: 3635 mean_circ: 0.932699
Ran for 108050 frames Cell Events: 3679 mean_circ: 0.932755
Ran for 109050 frames Cell Events: 3719 mean_circ: 0.93276
Ran for 110050 frames Cell Events: 3758 mean_circ: 0.932781
Ran for 110550 frames Cell Events: 3775 mean_circ: 0.932739
Ran for 112050 frames Cell Events: 3816 mean_circ: 0.932588
Ran for 112550 frames Cell Events: 3828 mean_circ: 0.932555
Ran for 113050 frames Cell Events: 3840 mean_circ: 0.932566
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Ran for 115050 frames Cell Events: 3920 mean_circ: 0.932316
Ran for 115550 frames Cell Events: 3937 mean_circ: 0.932285
Ran for 116050 frames Cell Events: 3957 mean_circ: 0.932286
Ran for 117050 frames Cell Events: 3998 mean_circ: 0.932304
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Ran for 118050 frames Cell Events: 4032 mean_circ: 0.932285
Ran for 119550 frames Cell Events: 4098 mean_circ: 0.932152
Ran for 120050 frames Cell Events: 4118 mean_circ: 0.932184
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Ran for 122550 frames Cell Events: 4196 mean_circ: 0.93236
Ran for 123050 frames Cell Events: 4205 mean_circ: 0.932373
Ran for 123550 frames Cell Events: 4225 mean_circ: 0.932345
Ran for 124550 frames Cell Events: 4261 mean_circ: 0.932317
Ran for 126050 frames Cell Events: 4319 mean_circ: 0.932349
Ran for 126550 frames Cell Events: 4327 mean_circ: 0.93234
Ran for 127050 frames Cell Events: 4342 mean_circ: 0.932361
Ran for 127550 frames Cell Events: 4357 mean_circ: 0.932386
Ran for 128550 frames Cell Events: 4399 mean_circ: 0.932495
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Ran for 133050 frames Cell Events: 4542 mean_circ: 0.932433
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Ran for 140050 frames Cell Events: 4776 mean_circ: 0.9324
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Ran for 141050 frames Cell Events: 4815 mean_circ: 0.93247
Ran for 142050 frames Cell Events: 4849 mean_circ: 0.932398
Ran for 142550 frames Cell Events: 4862 mean_circ: 0.932462
Ran for 143050 frames Cell Events: 4881 mean_circ: 0.932508
Ran for 144050 frames Cell Events: 4914 mean_circ: 0.932492
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Ran for 146050 frames Cell Events: 4996 mean_circ: 0.932463
Ran for 148050 frames Cell Events: 5088 mean_circ: 0.932366
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Ran for 151550 frames Cell Events: 5206 mean_circ: 0.932448
Ran for 152050 frames Cell Events: 5222 mean_circ: 0.932476
Ran for 152550 frames Cell Events: 5232 mean_circ: 0.932435
Ran for 154050 frames Cell Events: 5306 mean_circ: 0.932324
Ran for 154550 frames Cell Events: 5315 mean_circ: 0.932339
Ran for 157050 frames Cell Events: 5425 mean_circ: 0.932377
Ran for 157550 frames Cell Events: 5442 mean_circ: 0.932392
Ran for 158050 frames Cell Events: 5465 mean_circ: 0.932432
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Ran for 159050 frames Cell Events: 5503 mean_circ: 0.932479
Ran for 161550 frames Cell Events: 5602 mean_circ: 0.932439
Ran for 162050 frames Cell Events: 5621 mean_circ: 0.93248
Ran for 163050 frames Cell Events: 5650 mean_circ: 0.932405
Ran for 164050 frames Cell Events: 5690 mean_circ: 0.932438
Ran for 164550 frames Cell Events: 5707 mean_circ: 0.932477
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Ran for 166050 frames Cell Events: 5742 mean_circ: 0.932432
Ran for 167050 frames Cell Events: 5785 mean_circ: 0.932523
Ran for 167550 frames Cell Events: 5809 mean_circ: 0.932569
Ran for 168550 frames Cell Events: 5847 mean_circ: 0.932521
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Ran for 171550 frames Cell Events: 5944 mean_circ: 0.932561
Ran for 172050 frames Cell Events: 5959 mean_circ: 0.932551
Ran for 173550 frames Cell Events: 6033 mean_circ: 0.932418
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Ran for 225550 frames Cell Events: 7829 mean_circ: 0.932578
Ran for 226050 frames Cell Events: 7853 mean_circ: 0.93254
Ran for 226550 frames Cell Events: 7875 mean_circ: 0.93249
Ran for 227550 frames Cell Events: 7908 mean_circ: 0.932436
Ran for 228550 frames Cell Events: 7943 mean_circ: 0.932438
Ran for 229050 frames Cell Events: 7967 mean_circ: 0.932458
Ran for 230050 frames Cell Events: 7998 mean_circ: 0.932478
Ran for 232550 frames Cell Events: 8093 mean_circ: 0.932534
End this measurment..
Stopping time: 2.37038e+009
****************************************
Measurement finished
Mean Circularitiy: 0.932535
Mean Area: 624.639
Mean FL-1 intensity: 3370.8
Mean FL-2 intensity: 4591.1
Mean FL-3 intensity: 0
frames processed: 231092
Total Time: 116617ms
Number of Runs (new bg img) 0
Frames collected: 233052
Skipped frames: 65765
Rescued Frames: 6118007
Samples DAQ: 58263803
Samples written: 116525862
Samples per Channel: 58262931
FPS: 1998.44
Samples per second: 499610
Expected samples/s: 500000
Events: 8102
Events per second: 69.4754
jumped back (PMT): 34
#Frames read, frequency
# 0 frames :349200
# 1 frames :99591
# 2 frames :4204
# 3 frames :1746
# 4 frames :1117
# 5 frames :640
# 6 frames :1145
# 7 frames :1510
# 8 frames :1185
# 9 frames :790
# 10 frames :665
# 11 frames :698
# 12 frames :557
# 13 frames :411
# 14 frames :336
# 15 frames :298
# 16 frames :282
# 17 frames :263
# 18 frames :206
# 19 frames :191
# 20 frames :158
# 21 frames :133
# 22 frames :98
# 23 frames :74
# 24 frames :73
# 25 frames :59
# 26 frames :39
# 27 frames :42
# 28 frames :32
# 29 frames :34
# 30 frames :31
# 31 frames :26
# 32 frames :18
# 33 frames :21
# 34 frames :16
# 35 frames :17
# 36 frames :6
# 37 frames :9
# 38 frames :4
# 39 frames :7
# 40 frames :11
# 41 frames :1
# 42 frames :4
# 43 frames :4
# 44 frames :6
# 45 frames :6
# 46 frames :4
# 47 frames :2
# 48 frames :3
# 49 frames :5
# 50 frames :2
# 51 frames :2
# 52 frames :1
# 53 frames :2
# 54 frames :2
# 56 frames :1
# 57 frames :1
# 58 frames :1
# 60 frames :1
# 64 frames :1
# 68 frames :1
# 74 frames :1
# 78 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2223
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0