Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.25.08
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2a78115d8766bd616c99f37fe646d9a2",
"name": "M1_20170611_1922_dsRNA_Q6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:25:30"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8507 (max 8508) in '8507'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2803
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.37238e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 14 mean_circ: 0.917414
Ran for 1550 frames Cell Events: 45 mean_circ: 0.928178
Ran for 2050 frames Cell Events: 62 mean_circ: 0.930272
Ran for 2550 frames Cell Events: 83 mean_circ: 0.927138
Ran for 3050 frames Cell Events: 96 mean_circ: 0.926295
Ran for 3550 frames Cell Events: 112 mean_circ: 0.9249
Ran for 4050 frames Cell Events: 130 mean_circ: 0.925649
Ran for 4550 frames Cell Events: 140 mean_circ: 0.921291
Ran for 5050 frames Cell Events: 155 mean_circ: 0.920287
Ran for 5550 frames Cell Events: 169 mean_circ: 0.92128
Ran for 6050 frames Cell Events: 180 mean_circ: 0.922459
Ran for 7050 frames Cell Events: 218 mean_circ: 0.923269
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Ran for 12550 frames Cell Events: 391 mean_circ: 0.925485
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Ran for 63550 frames Cell Events: 1948 mean_circ: 0.926079
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Ran for 92050 frames Cell Events: 2750 mean_circ: 0.926554
Ran for 92550 frames Cell Events: 2769 mean_circ: 0.926564
Ran for 93050 frames Cell Events: 2783 mean_circ: 0.926439
Ran for 94050 frames Cell Events: 2819 mean_circ: 0.926602
Ran for 94550 frames Cell Events: 2836 mean_circ: 0.926523
Ran for 95550 frames Cell Events: 2876 mean_circ: 0.92639
Ran for 96050 frames Cell Events: 2886 mean_circ: 0.926442
Ran for 96550 frames Cell Events: 2906 mean_circ: 0.926352
Ran for 97050 frames Cell Events: 2918 mean_circ: 0.92638
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Ran for 100050 frames Cell Events: 2988 mean_circ: 0.926389
Ran for 100550 frames Cell Events: 2998 mean_circ: 0.926371
Ran for 101050 frames Cell Events: 3009 mean_circ: 0.926385
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Ran for 102050 frames Cell Events: 3033 mean_circ: 0.926479
Ran for 102550 frames Cell Events: 3047 mean_circ: 0.926494
Ran for 103050 frames Cell Events: 3058 mean_circ: 0.926457
Ran for 103550 frames Cell Events: 3073 mean_circ: 0.926513
Ran for 104050 frames Cell Events: 3087 mean_circ: 0.926388
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Ran for 106550 frames Cell Events: 3153 mean_circ: 0.926285
Ran for 107550 frames Cell Events: 3194 mean_circ: 0.926241
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Ran for 122050 frames Cell Events: 3666 mean_circ: 0.926844
Ran for 122550 frames Cell Events: 3676 mean_circ: 0.926821
Ran for 123050 frames Cell Events: 3690 mean_circ: 0.926875
Ran for 123550 frames Cell Events: 3698 mean_circ: 0.926913
Ran for 124550 frames Cell Events: 3734 mean_circ: 0.926979
Ran for 125050 frames Cell Events: 3749 mean_circ: 0.926943
Ran for 125550 frames Cell Events: 3761 mean_circ: 0.926855
Ran for 126550 frames Cell Events: 3806 mean_circ: 0.926861
Ran for 127050 frames Cell Events: 3823 mean_circ: 0.926867
Ran for 127550 frames Cell Events: 3835 mean_circ: 0.92684
Ran for 128050 frames Cell Events: 3858 mean_circ: 0.926785
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Ran for 131050 frames Cell Events: 3957 mean_circ: 0.926808
Ran for 131550 frames Cell Events: 3974 mean_circ: 0.926894
Ran for 132050 frames Cell Events: 3994 mean_circ: 0.926851
Ran for 132550 frames Cell Events: 4006 mean_circ: 0.926899
Ran for 133550 frames Cell Events: 4035 mean_circ: 0.926974
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Ran for 188550 frames Cell Events: 5689 mean_circ: 0.927191
Ran for 189050 frames Cell Events: 5709 mean_circ: 0.927222
Ran for 189550 frames Cell Events: 5724 mean_circ: 0.927202
Ran for 190050 frames Cell Events: 5735 mean_circ: 0.927234
Ran for 190550 frames Cell Events: 5752 mean_circ: 0.927248
Ran for 191050 frames Cell Events: 5768 mean_circ: 0.927269
Ran for 191550 frames Cell Events: 5780 mean_circ: 0.927284
Ran for 192550 frames Cell Events: 5822 mean_circ: 0.927379
Ran for 193050 frames Cell Events: 5837 mean_circ: 0.927351
Ran for 193550 frames Cell Events: 5851 mean_circ: 0.927298
Ran for 194550 frames Cell Events: 5879 mean_circ: 0.927325
Ran for 195050 frames Cell Events: 5896 mean_circ: 0.927307
Ran for 197550 frames Cell Events: 5956 mean_circ: 0.927313
Ran for 198050 frames Cell Events: 5971 mean_circ: 0.927318
Ran for 198550 frames Cell Events: 5986 mean_circ: 0.927316
Ran for 201050 frames Cell Events: 6053 mean_circ: 0.927271
Ran for 201550 frames Cell Events: 6072 mean_circ: 0.927268
Ran for 202050 frames Cell Events: 6088 mean_circ: 0.927201
Ran for 203050 frames Cell Events: 6120 mean_circ: 0.927181
Ran for 205550 frames Cell Events: 6202 mean_circ: 0.927178
Ran for 206550 frames Cell Events: 6227 mean_circ: 0.927134
Ran for 209050 frames Cell Events: 6333 mean_circ: 0.927064
Ran for 209550 frames Cell Events: 6362 mean_circ: 0.92704
Ran for 210050 frames Cell Events: 6367 mean_circ: 0.926984
Ran for 212050 frames Cell Events: 6438 mean_circ: 0.927074
Ran for 212550 frames Cell Events: 6448 mean_circ: 0.927069
Ran for 213050 frames Cell Events: 6465 mean_circ: 0.927076
Ran for 213550 frames Cell Events: 6474 mean_circ: 0.927112
Ran for 214050 frames Cell Events: 6490 mean_circ: 0.927142
Ran for 214550 frames Cell Events: 6506 mean_circ: 0.927142
Ran for 215050 frames Cell Events: 6533 mean_circ: 0.927035
Ran for 215550 frames Cell Events: 6551 mean_circ: 0.927041
Ran for 216050 frames Cell Events: 6568 mean_circ: 0.927006
Ran for 216550 frames Cell Events: 6575 mean_circ: 0.92702
Ran for 217050 frames Cell Events: 6590 mean_circ: 0.92703
Ran for 217550 frames Cell Events: 6606 mean_circ: 0.926965
Ran for 218050 frames Cell Events: 6619 mean_circ: 0.926972
Ran for 219550 frames Cell Events: 6659 mean_circ: 0.92703
Ran for 220050 frames Cell Events: 6672 mean_circ: 0.927068
Ran for 220550 frames Cell Events: 6688 mean_circ: 0.927068
Ran for 221550 frames Cell Events: 6733 mean_circ: 0.927106
Ran for 222550 frames Cell Events: 6775 mean_circ: 0.927143
Ran for 223550 frames Cell Events: 6821 mean_circ: 0.927166
Ran for 224050 frames Cell Events: 6841 mean_circ: 0.927124
Ran for 224550 frames Cell Events: 6855 mean_circ: 0.927132
Ran for 225550 frames Cell Events: 6889 mean_circ: 0.927148
Ran for 226550 frames Cell Events: 6917 mean_circ: 0.927161
Ran for 227050 frames Cell Events: 6927 mean_circ: 0.927152
Ran for 227550 frames Cell Events: 6942 mean_circ: 0.927186
Ran for 228050 frames Cell Events: 6946 mean_circ: 0.927197
Ran for 228550 frames Cell Events: 6961 mean_circ: 0.927227
Ran for 229050 frames Cell Events: 6971 mean_circ: 0.927212
Ran for 230050 frames Cell Events: 6997 mean_circ: 0.927199
Ran for 230550 frames Cell Events: 7018 mean_circ: 0.927192
Ran for 231050 frames Cell Events: 7035 mean_circ: 0.927151
Ran for 232050 frames Cell Events: 7064 mean_circ: 0.92718
Ran for 232550 frames Cell Events: 7075 mean_circ: 0.927184
Ran for 233050 frames Cell Events: 7085 mean_circ: 0.927201
Ran for 233550 frames Cell Events: 7097 mean_circ: 0.927235
Ran for 234550 frames Cell Events: 7133 mean_circ: 0.927204
Ran for 235050 frames Cell Events: 7155 mean_circ: 0.927195
Ran for 236050 frames Cell Events: 7181 mean_circ: 0.927159
Ran for 236550 frames Cell Events: 7198 mean_circ: 0.927206
Ran for 237050 frames Cell Events: 7204 mean_circ: 0.927236
Ran for 238050 frames Cell Events: 7238 mean_circ: 0.927242
Ran for 239050 frames Cell Events: 7274 mean_circ: 0.927223
Ran for 240550 frames Cell Events: 7331 mean_circ: 0.927247
Ran for 241050 frames Cell Events: 7348 mean_circ: 0.92727
Ran for 241550 frames Cell Events: 7355 mean_circ: 0.927298
Ran for 242050 frames Cell Events: 7377 mean_circ: 0.927298
Ran for 242550 frames Cell Events: 7399 mean_circ: 0.927317
Ran for 243050 frames Cell Events: 7419 mean_circ: 0.927332
Ran for 243550 frames Cell Events: 7428 mean_circ: 0.927319
Ran for 244050 frames Cell Events: 7448 mean_circ: 0.927316
Ran for 244550 frames Cell Events: 7461 mean_circ: 0.927306
Ran for 245050 frames Cell Events: 7478 mean_circ: 0.927283
Ran for 245550 frames Cell Events: 7488 mean_circ: 0.927244
Ran for 246050 frames Cell Events: 7510 mean_circ: 0.927188
Ran for 246550 frames Cell Events: 7533 mean_circ: 0.92717
Ran for 247050 frames Cell Events: 7545 mean_circ: 0.927088
Ran for 247550 frames Cell Events: 7559 mean_circ: 0.927099
Ran for 248550 frames Cell Events: 7584 mean_circ: 0.927062
Ran for 249050 frames Cell Events: 7590 mean_circ: 0.927076
Ran for 249550 frames Cell Events: 7600 mean_circ: 0.927071
Ran for 250550 frames Cell Events: 7647 mean_circ: 0.927095
Ran for 251050 frames Cell Events: 7676 mean_circ: 0.927153
Ran for 252050 frames Cell Events: 7718 mean_circ: 0.927146
Ran for 252550 frames Cell Events: 7740 mean_circ: 0.927159
Ran for 253050 frames Cell Events: 7758 mean_circ: 0.927153
Ran for 253550 frames Cell Events: 7771 mean_circ: 0.927153
Ran for 254050 frames Cell Events: 7788 mean_circ: 0.927173
Ran for 254550 frames Cell Events: 7815 mean_circ: 0.927215
Ran for 255050 frames Cell Events: 7848 mean_circ: 0.927208
Ran for 255550 frames Cell Events: 7868 mean_circ: 0.927112
Ran for 256050 frames Cell Events: 7879 mean_circ: 0.927127
Ran for 256550 frames Cell Events: 7888 mean_circ: 0.927122
Ran for 257050 frames Cell Events: 7903 mean_circ: 0.927156
Ran for 257550 frames Cell Events: 7918 mean_circ: 0.927178
Ran for 258050 frames Cell Events: 7937 mean_circ: 0.927159
Ran for 258550 frames Cell Events: 7952 mean_circ: 0.927173
Ran for 259050 frames Cell Events: 7973 mean_circ: 0.927132
Ran for 259550 frames Cell Events: 7988 mean_circ: 0.927153
Ran for 260050 frames Cell Events: 8004 mean_circ: 0.92713
Ran for 260550 frames Cell Events: 8019 mean_circ: 0.927134
Ran for 261050 frames Cell Events: 8033 mean_circ: 0.927146
Ran for 261550 frames Cell Events: 8049 mean_circ: 0.927149
Ran for 262050 frames Cell Events: 8058 mean_circ: 0.927167
Ran for 262550 frames Cell Events: 8075 mean_circ: 0.927196
Ran for 263550 frames Cell Events: 8100 mean_circ: 0.927189
Ran for 264050 frames Cell Events: 8107 mean_circ: 0.92719
Ran for 264550 frames Cell Events: 8119 mean_circ: 0.927167
Ran for 265550 frames Cell Events: 8150 mean_circ: 0.927164
Ran for 266050 frames Cell Events: 8157 mean_circ: 0.927179
Ran for 266550 frames Cell Events: 8171 mean_circ: 0.92717
Ran for 267050 frames Cell Events: 8189 mean_circ: 0.92718
Ran for 267550 frames Cell Events: 8206 mean_circ: 0.927162
Ran for 268550 frames Cell Events: 8227 mean_circ: 0.927136
Ran for 269550 frames Cell Events: 8260 mean_circ: 0.927141
Ran for 270050 frames Cell Events: 8279 mean_circ: 0.927142
Ran for 270550 frames Cell Events: 8287 mean_circ: 0.927138
Ran for 271550 frames Cell Events: 8317 mean_circ: 0.927126
Ran for 272050 frames Cell Events: 8322 mean_circ: 0.927125
Ran for 272550 frames Cell Events: 8339 mean_circ: 0.927107
Ran for 273550 frames Cell Events: 8377 mean_circ: 0.927084
Ran for 274050 frames Cell Events: 8391 mean_circ: 0.927096
Ran for 275550 frames Cell Events: 8433 mean_circ: 0.927119
Ran for 276050 frames Cell Events: 8449 mean_circ: 0.927094
Ran for 277050 frames Cell Events: 8493 mean_circ: 0.927112
End this measurment..
Stopping time: 2.37252e+009
****************************************
Measurement finished
Mean Circularitiy: 0.927104
Mean Area: 625.601
Mean FL-1 intensity: 3165.63
Mean FL-2 intensity: 4400.12
Mean FL-3 intensity: 0
frames processed: 276805
Total Time: 138790ms
Number of Runs (new bg img) 0
Frames collected: 277418
Skipped frames: 76715
Rescued Frames: 6707053
Samples DAQ: 69354419
Samples written: 138708716
Samples per Channel: 69354358
FPS: 1998.83
Samples per second: 499707
Expected samples/s: 500000
Events: 8508
Events per second: 61.3012
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :396078
# 1 frames :138051
# 2 frames :5880
# 3 frames :2273
# 4 frames :1439
# 5 frames :710
# 6 frames :1283
# 7 frames :2029
# 8 frames :1235
# 9 frames :860
# 10 frames :1052
# 11 frames :724
# 12 frames :657
# 13 frames :431
# 14 frames :343
# 15 frames :318
# 16 frames :264
# 17 frames :224
# 18 frames :196
# 19 frames :153
# 20 frames :98
# 21 frames :67
# 22 frames :68
# 23 frames :47
# 24 frames :47
# 25 frames :50
# 26 frames :27
# 27 frames :28
# 28 frames :24
# 29 frames :20
# 30 frames :30
# 31 frames :15
# 32 frames :15
# 33 frames :8
# 34 frames :9
# 35 frames :8
# 36 frames :10
# 37 frames :3
# 38 frames :6
# 39 frames :2
# 40 frames :3
# 41 frames :1
# 42 frames :5
# 43 frames :1
# 44 frames :1
# 45 frames :2
# 46 frames :1
# 47 frames :2
# 48 frames :1
# 49 frames :2
# 52 frames :1
# 53 frames :1
# 56 frames :1
# 57 frames :1
# 59 frames :1
# 68 frames :1
# 89 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2803
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0