Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.27.27
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e7b79aedaf961f78dc8f80b87a48d80d",
"name": "M1_20170611_2001_dsRNA_Q1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:27:49"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8675 (max 8676) in '8675'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2811
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.37468e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 10 mean_circ: 0.938204
Ran for 1050 frames Cell Events: 22 mean_circ: 0.943065
Ran for 1550 frames Cell Events: 35 mean_circ: 0.927639
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Ran for 3050 frames Cell Events: 84 mean_circ: 0.937555
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Ran for 4550 frames Cell Events: 142 mean_circ: 0.941151
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Ran for 92050 frames Cell Events: 3037 mean_circ: 0.941391
Ran for 92550 frames Cell Events: 3057 mean_circ: 0.941409
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Ran for 100550 frames Cell Events: 3326 mean_circ: 0.941892
Ran for 102050 frames Cell Events: 3391 mean_circ: 0.941673
Ran for 102550 frames Cell Events: 3410 mean_circ: 0.941525
Ran for 103050 frames Cell Events: 3426 mean_circ: 0.941582
Ran for 103550 frames Cell Events: 3443 mean_circ: 0.941607
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Ran for 122050 frames Cell Events: 4061 mean_circ: 0.941742
Ran for 122550 frames Cell Events: 4072 mean_circ: 0.941747
Ran for 124050 frames Cell Events: 4122 mean_circ: 0.941784
Ran for 126050 frames Cell Events: 4179 mean_circ: 0.941844
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Ran for 131550 frames Cell Events: 4357 mean_circ: 0.941986
Ran for 134050 frames Cell Events: 4449 mean_circ: 0.942171
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Ran for 141550 frames Cell Events: 4693 mean_circ: 0.942473
Ran for 143050 frames Cell Events: 4743 mean_circ: 0.942402
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Ran for 146550 frames Cell Events: 4866 mean_circ: 0.942327
Ran for 147050 frames Cell Events: 4880 mean_circ: 0.942249
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Ran for 195550 frames Cell Events: 6555 mean_circ: 0.942383
Ran for 196550 frames Cell Events: 6585 mean_circ: 0.942409
Ran for 197050 frames Cell Events: 6601 mean_circ: 0.942382
Ran for 197550 frames Cell Events: 6622 mean_circ: 0.942389
Ran for 198050 frames Cell Events: 6631 mean_circ: 0.942392
Ran for 198550 frames Cell Events: 6642 mean_circ: 0.942391
Ran for 199050 frames Cell Events: 6655 mean_circ: 0.942394
Ran for 199550 frames Cell Events: 6674 mean_circ: 0.942443
Ran for 201050 frames Cell Events: 6730 mean_circ: 0.942423
Ran for 202050 frames Cell Events: 6759 mean_circ: 0.942428
Ran for 202550 frames Cell Events: 6769 mean_circ: 0.942355
Ran for 203050 frames Cell Events: 6785 mean_circ: 0.942364
Ran for 203550 frames Cell Events: 6799 mean_circ: 0.942389
Ran for 204550 frames Cell Events: 6841 mean_circ: 0.942422
Ran for 205050 frames Cell Events: 6857 mean_circ: 0.942426
Ran for 205550 frames Cell Events: 6878 mean_circ: 0.942443
Ran for 206550 frames Cell Events: 6912 mean_circ: 0.942412
Ran for 207050 frames Cell Events: 6926 mean_circ: 0.942412
Ran for 207550 frames Cell Events: 6942 mean_circ: 0.942435
Ran for 208550 frames Cell Events: 6978 mean_circ: 0.942419
Ran for 210050 frames Cell Events: 7027 mean_circ: 0.942436
Ran for 211050 frames Cell Events: 7078 mean_circ: 0.942502
Ran for 212050 frames Cell Events: 7116 mean_circ: 0.942509
Ran for 212550 frames Cell Events: 7138 mean_circ: 0.942492
Ran for 213050 frames Cell Events: 7152 mean_circ: 0.942501
Ran for 213550 frames Cell Events: 7165 mean_circ: 0.942517
Ran for 214050 frames Cell Events: 7179 mean_circ: 0.942526
Ran for 215050 frames Cell Events: 7211 mean_circ: 0.942508
Ran for 216050 frames Cell Events: 7269 mean_circ: 0.942409
Ran for 216550 frames Cell Events: 7290 mean_circ: 0.942418
Ran for 217550 frames Cell Events: 7315 mean_circ: 0.942395
Ran for 219050 frames Cell Events: 7379 mean_circ: 0.942478
Ran for 220550 frames Cell Events: 7427 mean_circ: 0.942543
Ran for 221050 frames Cell Events: 7441 mean_circ: 0.942504
Ran for 221550 frames Cell Events: 7458 mean_circ: 0.94253
Ran for 222550 frames Cell Events: 7488 mean_circ: 0.942574
Ran for 223050 frames Cell Events: 7506 mean_circ: 0.942583
Ran for 223550 frames Cell Events: 7529 mean_circ: 0.942533
Ran for 224550 frames Cell Events: 7572 mean_circ: 0.942525
Ran for 225050 frames Cell Events: 7594 mean_circ: 0.942515
Ran for 225550 frames Cell Events: 7608 mean_circ: 0.942524
Ran for 226050 frames Cell Events: 7620 mean_circ: 0.942551
Ran for 226550 frames Cell Events: 7631 mean_circ: 0.94255
Ran for 227050 frames Cell Events: 7648 mean_circ: 0.942561
Ran for 227550 frames Cell Events: 7668 mean_circ: 0.942574
Ran for 229050 frames Cell Events: 7712 mean_circ: 0.942548
Ran for 230550 frames Cell Events: 7772 mean_circ: 0.942562
Ran for 231550 frames Cell Events: 7795 mean_circ: 0.942544
Ran for 232050 frames Cell Events: 7807 mean_circ: 0.942563
Ran for 232550 frames Cell Events: 7825 mean_circ: 0.942553
Ran for 233050 frames Cell Events: 7839 mean_circ: 0.942568
Ran for 234050 frames Cell Events: 7884 mean_circ: 0.942524
Ran for 234550 frames Cell Events: 7896 mean_circ: 0.942556
Ran for 235050 frames Cell Events: 7914 mean_circ: 0.942556
Ran for 236050 frames Cell Events: 7963 mean_circ: 0.942492
Ran for 236550 frames Cell Events: 7977 mean_circ: 0.942494
Ran for 237050 frames Cell Events: 8006 mean_circ: 0.942438
Ran for 238050 frames Cell Events: 8045 mean_circ: 0.942428
Ran for 238550 frames Cell Events: 8055 mean_circ: 0.942415
Ran for 239050 frames Cell Events: 8077 mean_circ: 0.94243
Ran for 239550 frames Cell Events: 8090 mean_circ: 0.942403
Ran for 240050 frames Cell Events: 8114 mean_circ: 0.94236
Ran for 240550 frames Cell Events: 8130 mean_circ: 0.942345
Ran for 241050 frames Cell Events: 8145 mean_circ: 0.942333
Ran for 243050 frames Cell Events: 8219 mean_circ: 0.942312
Ran for 243550 frames Cell Events: 8237 mean_circ: 0.942288
Ran for 244550 frames Cell Events: 8280 mean_circ: 0.942307
Ran for 245050 frames Cell Events: 8302 mean_circ: 0.942257
Ran for 245550 frames Cell Events: 8323 mean_circ: 0.942246
Ran for 246050 frames Cell Events: 8344 mean_circ: 0.94228
Ran for 246550 frames Cell Events: 8352 mean_circ: 0.94229
Ran for 247550 frames Cell Events: 8395 mean_circ: 0.942325
Ran for 248550 frames Cell Events: 8435 mean_circ: 0.942322
Ran for 249050 frames Cell Events: 8455 mean_circ: 0.942302
Ran for 250050 frames Cell Events: 8487 mean_circ: 0.942276
Ran for 251050 frames Cell Events: 8526 mean_circ: 0.942295
Ran for 251550 frames Cell Events: 8541 mean_circ: 0.942298
Ran for 252550 frames Cell Events: 8579 mean_circ: 0.942293
Ran for 255550 frames Cell Events: 8669 mean_circ: 0.942232
End this measurment..
Stopping time: 2.37481e+009
****************************************
Measurement finished
Mean Circularitiy: 0.942205
Mean Area: 647.458
Mean FL-1 intensity: 2968.6
Mean FL-2 intensity: 3636.04
Mean FL-3 intensity: 0
frames processed: 255065
Total Time: 127983ms
Number of Runs (new bg img) 0
Frames collected: 255800
Skipped frames: 78547
Rescued Frames: 7184234
Samples DAQ: 63950571
Samples written: 127899708
Samples per Channel: 63949854
FPS: 1998.71
Samples per second: 499676
Expected samples/s: 500000
Events: 8676
Events per second: 67.7904
jumped back (PMT): 5661
#Frames read, frequency
# 0 frames :364425
# 1 frames :127778
# 2 frames :5746
# 3 frames :1953
# 4 frames :1208
# 5 frames :517
# 6 frames :1359
# 7 frames :2099
# 8 frames :1263
# 9 frames :773
# 10 frames :948
# 11 frames :691
# 12 frames :635
# 13 frames :367
# 14 frames :315
# 15 frames :276
# 16 frames :233
# 17 frames :190
# 18 frames :170
# 19 frames :106
# 20 frames :78
# 21 frames :68
# 22 frames :51
# 23 frames :44
# 24 frames :41
# 25 frames :33
# 26 frames :30
# 27 frames :20
# 28 frames :27
# 29 frames :22
# 30 frames :15
# 31 frames :11
# 32 frames :11
# 33 frames :7
# 34 frames :7
# 35 frames :5
# 36 frames :3
# 37 frames :3
# 38 frames :3
# 39 frames :3
# 40 frames :6
# 41 frames :3
# 42 frames :1
# 43 frames :3
# 45 frames :1
# 48 frames :2
# 51 frames :1
# 66 frames :1
# 85 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2811
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0