Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.31.12
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a6cbe9c78703d59e5f244bfaad2051a1",
"name": "M1_20170612_1330_dsRNA_R8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:31:35"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8774 (max 8776) in '8774'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5933
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.43766e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 14 mean_circ: 0.851513
Ran for 1050 frames Cell Events: 32 mean_circ: 0.919343
Ran for 1550 frames Cell Events: 46 mean_circ: 0.927492
Ran for 2050 frames Cell Events: 62 mean_circ: 0.932742
Ran for 2550 frames Cell Events: 74 mean_circ: 0.933967
Ran for 3050 frames Cell Events: 90 mean_circ: 0.935849
Ran for 3550 frames Cell Events: 113 mean_circ: 0.93879
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Ran for 7050 frames Cell Events: 229 mean_circ: 0.943327
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Ran for 83550 frames Cell Events: 2913 mean_circ: 0.947333
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Ran for 94050 frames Cell Events: 3308 mean_circ: 0.947483
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Ran for 97050 frames Cell Events: 3445 mean_circ: 0.947227
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Ran for 102050 frames Cell Events: 3637 mean_circ: 0.947068
Ran for 103050 frames Cell Events: 3668 mean_circ: 0.94712
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Ran for 104050 frames Cell Events: 3692 mean_circ: 0.947187
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Ran for 106550 frames Cell Events: 3784 mean_circ: 0.947172
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Ran for 108050 frames Cell Events: 3836 mean_circ: 0.947178
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Ran for 121550 frames Cell Events: 4350 mean_circ: 0.947154
Ran for 122550 frames Cell Events: 4391 mean_circ: 0.947145
Ran for 123050 frames Cell Events: 4404 mean_circ: 0.947127
Ran for 125050 frames Cell Events: 4472 mean_circ: 0.947083
Ran for 125550 frames Cell Events: 4486 mean_circ: 0.947054
Ran for 126050 frames Cell Events: 4510 mean_circ: 0.947044
Ran for 127550 frames Cell Events: 4565 mean_circ: 0.947054
Ran for 128050 frames Cell Events: 4580 mean_circ: 0.947063
Ran for 128550 frames Cell Events: 4591 mean_circ: 0.947101
Ran for 129050 frames Cell Events: 4605 mean_circ: 0.947121
Ran for 130550 frames Cell Events: 4688 mean_circ: 0.947049
Ran for 131550 frames Cell Events: 4737 mean_circ: 0.947039
Ran for 132050 frames Cell Events: 4766 mean_circ: 0.947056
Ran for 132550 frames Cell Events: 4785 mean_circ: 0.947019
Ran for 133050 frames Cell Events: 4800 mean_circ: 0.946976
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Ran for 142550 frames Cell Events: 5121 mean_circ: 0.947095
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Ran for 146550 frames Cell Events: 5284 mean_circ: 0.946986
Ran for 147050 frames Cell Events: 5296 mean_circ: 0.946973
Ran for 147550 frames Cell Events: 5311 mean_circ: 0.947004
Ran for 148050 frames Cell Events: 5337 mean_circ: 0.947025
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Ran for 198050 frames Cell Events: 7229 mean_circ: 0.947064
Ran for 198550 frames Cell Events: 7243 mean_circ: 0.947082
Ran for 199050 frames Cell Events: 7260 mean_circ: 0.947085
Ran for 200050 frames Cell Events: 7291 mean_circ: 0.947095
Ran for 200550 frames Cell Events: 7305 mean_circ: 0.947119
Ran for 201550 frames Cell Events: 7333 mean_circ: 0.947133
Ran for 202050 frames Cell Events: 7342 mean_circ: 0.947128
Ran for 203050 frames Cell Events: 7378 mean_circ: 0.947157
Ran for 203550 frames Cell Events: 7393 mean_circ: 0.947163
Ran for 205550 frames Cell Events: 7472 mean_circ: 0.947149
Ran for 207050 frames Cell Events: 7532 mean_circ: 0.947188
Ran for 207550 frames Cell Events: 7542 mean_circ: 0.947182
Ran for 208050 frames Cell Events: 7550 mean_circ: 0.94718
Ran for 209550 frames Cell Events: 7596 mean_circ: 0.947179
Ran for 210550 frames Cell Events: 7626 mean_circ: 0.947185
Ran for 211050 frames Cell Events: 7645 mean_circ: 0.947165
Ran for 211550 frames Cell Events: 7666 mean_circ: 0.947169
Ran for 212050 frames Cell Events: 7684 mean_circ: 0.947172
Ran for 212550 frames Cell Events: 7695 mean_circ: 0.947172
Ran for 213050 frames Cell Events: 7728 mean_circ: 0.947211
Ran for 213550 frames Cell Events: 7747 mean_circ: 0.947206
Ran for 214050 frames Cell Events: 7761 mean_circ: 0.947209
Ran for 214550 frames Cell Events: 7776 mean_circ: 0.947187
Ran for 216550 frames Cell Events: 7857 mean_circ: 0.947209
Ran for 217050 frames Cell Events: 7884 mean_circ: 0.947226
Ran for 218050 frames Cell Events: 7931 mean_circ: 0.947206
Ran for 218550 frames Cell Events: 7941 mean_circ: 0.947198
Ran for 219050 frames Cell Events: 7964 mean_circ: 0.947215
Ran for 219550 frames Cell Events: 7979 mean_circ: 0.947214
Ran for 220050 frames Cell Events: 7999 mean_circ: 0.947234
Ran for 220550 frames Cell Events: 8020 mean_circ: 0.947233
Ran for 221050 frames Cell Events: 8033 mean_circ: 0.947222
Ran for 221550 frames Cell Events: 8051 mean_circ: 0.947243
Ran for 222050 frames Cell Events: 8067 mean_circ: 0.947248
Ran for 223050 frames Cell Events: 8109 mean_circ: 0.94721
Ran for 223550 frames Cell Events: 8125 mean_circ: 0.947208
Ran for 225050 frames Cell Events: 8201 mean_circ: 0.94718
Ran for 226050 frames Cell Events: 8237 mean_circ: 0.947219
Ran for 226550 frames Cell Events: 8258 mean_circ: 0.947228
Ran for 228050 frames Cell Events: 8320 mean_circ: 0.947253
Ran for 228550 frames Cell Events: 8328 mean_circ: 0.947236
Ran for 229050 frames Cell Events: 8348 mean_circ: 0.947224
Ran for 229550 frames Cell Events: 8370 mean_circ: 0.947163
Ran for 230050 frames Cell Events: 8392 mean_circ: 0.947168
Ran for 231050 frames Cell Events: 8439 mean_circ: 0.947188
Ran for 232550 frames Cell Events: 8492 mean_circ: 0.94721
Ran for 233550 frames Cell Events: 8546 mean_circ: 0.947165
Ran for 234050 frames Cell Events: 8563 mean_circ: 0.947163
Ran for 234550 frames Cell Events: 8583 mean_circ: 0.947184
Ran for 235050 frames Cell Events: 8596 mean_circ: 0.947173
Ran for 236050 frames Cell Events: 8645 mean_circ: 0.947191
Ran for 237050 frames Cell Events: 8689 mean_circ: 0.947188
Ran for 237550 frames Cell Events: 8702 mean_circ: 0.94717
Ran for 238050 frames Cell Events: 8720 mean_circ: 0.947176
Ran for 238550 frames Cell Events: 8739 mean_circ: 0.947168
End this measurment..
Stopping time: 2.43778e+009
****************************************
Measurement finished
Mean Circularitiy: 0.947165
Mean Area: 634.26
Mean FL-1 intensity: 2872.73
Mean FL-2 intensity: 3430.07
Mean FL-3 intensity: 0
frames processed: 239255
Total Time: 119892ms
Number of Runs (new bg img) 0
Frames collected: 239662
Skipped frames: 2426
Rescued Frames: 505794
Samples DAQ: 59916911
Samples written: 119830828
Samples per Channel: 59915414
FPS: 1998.98
Samples per second: 499744
Expected samples/s: 500000
Events: 8776
Events per second: 73.199
jumped back (PMT): 5517
#Frames read, frequency
# 0 frames :340228
# 1 frames :120617
# 2 frames :5459
# 3 frames :1840
# 4 frames :993
# 5 frames :469
# 6 frames :1533
# 7 frames :2049
# 8 frames :1129
# 9 frames :784
# 10 frames :1367
# 11 frames :636
# 12 frames :482
# 13 frames :285
# 14 frames :199
# 15 frames :205
# 16 frames :202
# 17 frames :126
# 18 frames :101
# 19 frames :87
# 20 frames :71
# 21 frames :60
# 22 frames :44
# 23 frames :50
# 24 frames :36
# 25 frames :38
# 26 frames :26
# 27 frames :17
# 28 frames :8
# 29 frames :15
# 30 frames :10
# 31 frames :12
# 32 frames :10
# 33 frames :6
# 34 frames :9
# 35 frames :3
# 36 frames :3
# 37 frames :2
# 38 frames :1
# 39 frames :7
# 40 frames :1
# 41 frames :2
# 42 frames :2
# 43 frames :2
# 44 frames :1
# 45 frames :7
# 46 frames :1
# 48 frames :2
# 50 frames :2
# 52 frames :2
# 67 frames :2
# 78 frames :1
# 91 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5933
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0