M1_20170612_1415_dsRNA_S3_faster.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.34.33
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0c7e4baaf8a16f3b8f29859ae2cf019d",
"name": "M1_20170612_1415_dsRNA_S3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:34:50"
}
}
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6912
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.44032e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.91466
Ran for 550 frames Cell Events: 7 mean_circ: 0.910766
Ran for 1050 frames Cell Events: 9 mean_circ: 0.913708
Ran for 1550 frames Cell Events: 10 mean_circ: 0.918178
Ran for 2550 frames Cell Events: 19 mean_circ: 0.916238
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Ran for 73050 frames Cell Events: 653 mean_circ: 0.910903
Ran for 73550 frames Cell Events: 660 mean_circ: 0.910976
Ran for 74050 frames Cell Events: 669 mean_circ: 0.910881
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Ran for 76550 frames Cell Events: 682 mean_circ: 0.911253
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Ran for 102550 frames Cell Events: 944 mean_circ: 0.911366
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Ran for 152550 frames Cell Events: 1439 mean_circ: 0.911197
Ran for 153050 frames Cell Events: 1446 mean_circ: 0.911074
Ran for 153550 frames Cell Events: 1450 mean_circ: 0.911092
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Ran for 551550 frames Cell Events: 5066 mean_circ: 0.907536
Ran for 552050 frames Cell Events: 5071 mean_circ: 0.907546
Ran for 554550 frames Cell Events: 5086 mean_circ: 0.90754
Ran for 555050 frames Cell Events: 5090 mean_circ: 0.907521
Ran for 555550 frames Cell Events: 5100 mean_circ: 0.907522
Ran for 556050 frames Cell Events: 5104 mean_circ: 0.907507
Ran for 556550 frames Cell Events: 5111 mean_circ: 0.907531
Ran for 557050 frames Cell Events: 5115 mean_circ: 0.907562
Ran for 557550 frames Cell Events: 5119 mean_circ: 0.907567
Ran for 558050 frames Cell Events: 5125 mean_circ: 0.907601
Ran for 558550 frames Cell Events: 5131 mean_circ: 0.907599
Ran for 559050 frames Cell Events: 5134 mean_circ: 0.907613
Ran for 559550 frames Cell Events: 5139 mean_circ: 0.907623
Ran for 560050 frames Cell Events: 5142 mean_circ: 0.907634
Ran for 560550 frames Cell Events: 5145 mean_circ: 0.907621
Ran for 561050 frames Cell Events: 5152 mean_circ: 0.907664
Ran for 561550 frames Cell Events: 5160 mean_circ: 0.90771
Ran for 562050 frames Cell Events: 5164 mean_circ: 0.907733
Ran for 562550 frames Cell Events: 5167 mean_circ: 0.907717
Ran for 563050 frames Cell Events: 5171 mean_circ: 0.90771
Ran for 563550 frames Cell Events: 5177 mean_circ: 0.907696
Ran for 564050 frames Cell Events: 5181 mean_circ: 0.907674
Ran for 564550 frames Cell Events: 5185 mean_circ: 0.907663
Ran for 565050 frames Cell Events: 5192 mean_circ: 0.907687
Ran for 565550 frames Cell Events: 5195 mean_circ: 0.907663
Ran for 566050 frames Cell Events: 5200 mean_circ: 0.907636
Ran for 566550 frames Cell Events: 5203 mean_circ: 0.907647
Ran for 567050 frames Cell Events: 5207 mean_circ: 0.907642
Ran for 567550 frames Cell Events: 5214 mean_circ: 0.907558
Ran for 568050 frames Cell Events: 5216 mean_circ: 0.907567
Ran for 568550 frames Cell Events: 5219 mean_circ: 0.907549
Ran for 569050 frames Cell Events: 5222 mean_circ: 0.907562
Ran for 569550 frames Cell Events: 5222 mean_circ: 0.907562
Ran for 570050 frames Cell Events: 5225 mean_circ: 0.907558
Ran for 570550 frames Cell Events: 5229 mean_circ: 0.907555
Ran for 571050 frames Cell Events: 5232 mean_circ: 0.90754
Ran for 571550 frames Cell Events: 5235 mean_circ: 0.907533
Ran for 572050 frames Cell Events: 5240 mean_circ: 0.907557
Ran for 572550 frames Cell Events: 5248 mean_circ: 0.907564
Ran for 573050 frames Cell Events: 5250 mean_circ: 0.907561
Ran for 573550 frames Cell Events: 5255 mean_circ: 0.907574
Ran for 574550 frames Cell Events: 5259 mean_circ: 0.907569
Ran for 575550 frames Cell Events: 5265 mean_circ: 0.907573
Ran for 576050 frames Cell Events: 5271 mean_circ: 0.907485
Ran for 576550 frames Cell Events: 5276 mean_circ: 0.907473
Ran for 577050 frames Cell Events: 5277 mean_circ: 0.907475
Ran for 577550 frames Cell Events: 5277 mean_circ: 0.907475
Ran for 578050 frames Cell Events: 5282 mean_circ: 0.907443
Ran for 579050 frames Cell Events: 5292 mean_circ: 0.907414
Ran for 579550 frames Cell Events: 5295 mean_circ: 0.907426
Ran for 580050 frames Cell Events: 5299 mean_circ: 0.907419
Ran for 580550 frames Cell Events: 5299 mean_circ: 0.907419
Ran for 581050 frames Cell Events: 5304 mean_circ: 0.907431
Ran for 581550 frames Cell Events: 5309 mean_circ: 0.907404
Ran for 582050 frames Cell Events: 5317 mean_circ: 0.907369
Ran for 582550 frames Cell Events: 5323 mean_circ: 0.907385
Ran for 583050 frames Cell Events: 5324 mean_circ: 0.907382
Ran for 583550 frames Cell Events: 5329 mean_circ: 0.907422
Ran for 584050 frames Cell Events: 5330 mean_circ: 0.907433
Ran for 585050 frames Cell Events: 5339 mean_circ: 0.907384
Ran for 585550 frames Cell Events: 5341 mean_circ: 0.907379
Ran for 586050 frames Cell Events: 5344 mean_circ: 0.907395
Ran for 586550 frames Cell Events: 5348 mean_circ: 0.907389
Ran for 587050 frames Cell Events: 5356 mean_circ: 0.907349
Ran for 587550 frames Cell Events: 5359 mean_circ: 0.907324
Ran for 588050 frames Cell Events: 5362 mean_circ: 0.907298
Ran for 588550 frames Cell Events: 5368 mean_circ: 0.907288
Ran for 589050 frames Cell Events: 5372 mean_circ: 0.907288
Ran for 589550 frames Cell Events: 5377 mean_circ: 0.90726
Ran for 590050 frames Cell Events: 5383 mean_circ: 0.907266
Ran for 590550 frames Cell Events: 5388 mean_circ: 0.90726
Ran for 591050 frames Cell Events: 5391 mean_circ: 0.907264
Ran for 591550 frames Cell Events: 5396 mean_circ: 0.907266
Ran for 592550 frames Cell Events: 5402 mean_circ: 0.907255
Ran for 593050 frames Cell Events: 5405 mean_circ: 0.907259
Ran for 593550 frames Cell Events: 5411 mean_circ: 0.907277
Ran for 594550 frames Cell Events: 5418 mean_circ: 0.907273
Ran for 595050 frames Cell Events: 5423 mean_circ: 0.907292
Ran for 595550 frames Cell Events: 5427 mean_circ: 0.907302
Ran for 596050 frames Cell Events: 5430 mean_circ: 0.907313
Ran for 596550 frames Cell Events: 5435 mean_circ: 0.907314
Ran for 597050 frames Cell Events: 5438 mean_circ: 0.907322
Ran for 597550 frames Cell Events: 5444 mean_circ: 0.907331
Ran for 598050 frames Cell Events: 5447 mean_circ: 0.907318
Ran for 598550 frames Cell Events: 5451 mean_circ: 0.907318
Ran for 599550 frames Cell Events: 5457 mean_circ: 0.907308
Ran for 600050 frames Cell Events: 5461 mean_circ: 0.907303
Ran for 601050 frames Cell Events: 5470 mean_circ: 0.907298
Ran for 601550 frames Cell Events: 5472 mean_circ: 0.907288
Ran for 602050 frames Cell Events: 5475 mean_circ: 0.907266
Ran for 602550 frames Cell Events: 5482 mean_circ: 0.907217
Ran for 603050 frames Cell Events: 5485 mean_circ: 0.907238
Ran for 603550 frames Cell Events: 5488 mean_circ: 0.907253
Ran for 604550 frames Cell Events: 5508 mean_circ: 0.907289
Ran for 605050 frames Cell Events: 5515 mean_circ: 0.907316
Ran for 605550 frames Cell Events: 5520 mean_circ: 0.907281
Ran for 606050 frames Cell Events: 5522 mean_circ: 0.907277
Ran for 606550 frames Cell Events: 5523 mean_circ: 0.907277
Ran for 607050 frames Cell Events: 5526 mean_circ: 0.907283
Ran for 607550 frames Cell Events: 5530 mean_circ: 0.907273
Ran for 608050 frames Cell Events: 5533 mean_circ: 0.907276
Ran for 608550 frames Cell Events: 5537 mean_circ: 0.907271
Ran for 609050 frames Cell Events: 5539 mean_circ: 0.907274
Ran for 609550 frames Cell Events: 5544 mean_circ: 0.907269
Ran for 610050 frames Cell Events: 5547 mean_circ: 0.907274
Ran for 610550 frames Cell Events: 5553 mean_circ: 0.907279
Ran for 611050 frames Cell Events: 5560 mean_circ: 0.907287
Ran for 611550 frames Cell Events: 5574 mean_circ: 0.907278
Ran for 612050 frames Cell Events: 5576 mean_circ: 0.9073
Ran for 612550 frames Cell Events: 5578 mean_circ: 0.907298
Ran for 613050 frames Cell Events: 5579 mean_circ: 0.907302
Ran for 613550 frames Cell Events: 5584 mean_circ: 0.907299
Ran for 614050 frames Cell Events: 5585 mean_circ: 0.907312
Ran for 614550 frames Cell Events: 5588 mean_circ: 0.9073
Ran for 615050 frames Cell Events: 5591 mean_circ: 0.907316
Ran for 615550 frames Cell Events: 5594 mean_circ: 0.907329
Ran for 616050 frames Cell Events: 5597 mean_circ: 0.907338
Ran for 616550 frames Cell Events: 5598 mean_circ: 0.907343
Ran for 617050 frames Cell Events: 5603 mean_circ: 0.90735
Ran for 617550 frames Cell Events: 5607 mean_circ: 0.907347
Ran for 618050 frames Cell Events: 5613 mean_circ: 0.907346
Ran for 619050 frames Cell Events: 5624 mean_circ: 0.907354
Ran for 619550 frames Cell Events: 5631 mean_circ: 0.907306
Ran for 620050 frames Cell Events: 5636 mean_circ: 0.907295
Ran for 620550 frames Cell Events: 5641 mean_circ: 0.907291
Ran for 621050 frames Cell Events: 5645 mean_circ: 0.907294
Ran for 621550 frames Cell Events: 5652 mean_circ: 0.907194
Ran for 622050 frames Cell Events: 5662 mean_circ: 0.907176
Ran for 623050 frames Cell Events: 5676 mean_circ: 0.907159
Ran for 623550 frames Cell Events: 5678 mean_circ: 0.907159
Ran for 624050 frames Cell Events: 5681 mean_circ: 0.907161
Ran for 624550 frames Cell Events: 5683 mean_circ: 0.907181
Ran for 625050 frames Cell Events: 5687 mean_circ: 0.907189
Ran for 625550 frames Cell Events: 5689 mean_circ: 0.907183
Ran for 626050 frames Cell Events: 5691 mean_circ: 0.907174
Ran for 627050 frames Cell Events: 5706 mean_circ: 0.907144
Ran for 627550 frames Cell Events: 5714 mean_circ: 0.90717
Ran for 628050 frames Cell Events: 5721 mean_circ: 0.907165
Ran for 628550 frames Cell Events: 5728 mean_circ: 0.90716
Ran for 629050 frames Cell Events: 5733 mean_circ: 0.907169
Ran for 629550 frames Cell Events: 5738 mean_circ: 0.907077
Ran for 630050 frames Cell Events: 5740 mean_circ: 0.907079
Ran for 630550 frames Cell Events: 5745 mean_circ: 0.907051
Ran for 631050 frames Cell Events: 5748 mean_circ: 0.907048
Ran for 631550 frames Cell Events: 5754 mean_circ: 0.90705
Ran for 632050 frames Cell Events: 5758 mean_circ: 0.907037
Ran for 632550 frames Cell Events: 5764 mean_circ: 0.907032
Ran for 633050 frames Cell Events: 5770 mean_circ: 0.907028
Ran for 633550 frames Cell Events: 5773 mean_circ: 0.907005
Ran for 634050 frames Cell Events: 5775 mean_circ: 0.906993
Ran for 634550 frames Cell Events: 5779 mean_circ: 0.906981
Ran for 635050 frames Cell Events: 5783 mean_circ: 0.906958
Ran for 635550 frames Cell Events: 5786 mean_circ: 0.906954
Ran for 636050 frames Cell Events: 5790 mean_circ: 0.906952
Ran for 636550 frames Cell Events: 5793 mean_circ: 0.906936
Ran for 637050 frames Cell Events: 5796 mean_circ: 0.906945
Ran for 637550 frames Cell Events: 5798 mean_circ: 0.90694
Ran for 638050 frames Cell Events: 5803 mean_circ: 0.906923
Ran for 638550 frames Cell Events: 5807 mean_circ: 0.906927
Ran for 639050 frames Cell Events: 5812 mean_circ: 0.906939
Ran for 639550 frames Cell Events: 5819 mean_circ: 0.906928
Ran for 640050 frames Cell Events: 5824 mean_circ: 0.906923
Ran for 640550 frames Cell Events: 5830 mean_circ: 0.90695
Ran for 641050 frames Cell Events: 5833 mean_circ: 0.90693
Ran for 641550 frames Cell Events: 5836 mean_circ: 0.906953
Ran for 642050 frames Cell Events: 5837 mean_circ: 0.906935
Ran for 642550 frames Cell Events: 5842 mean_circ: 0.90691
Ran for 643050 frames Cell Events: 5844 mean_circ: 0.90692
Ran for 644050 frames Cell Events: 5853 mean_circ: 0.906888
Ran for 644550 frames Cell Events: 5856 mean_circ: 0.906864
Ran for 645050 frames Cell Events: 5860 mean_circ: 0.906854
Ran for 645550 frames Cell Events: 5864 mean_circ: 0.906852
Ran for 646050 frames Cell Events: 5867 mean_circ: 0.906856
Ran for 646550 frames Cell Events: 5871 mean_circ: 0.906843
Ran for 647050 frames Cell Events: 5882 mean_circ: 0.906876
Ran for 647550 frames Cell Events: 5885 mean_circ: 0.906899
Ran for 648050 frames Cell Events: 5888 mean_circ: 0.906893
Ran for 648550 frames Cell Events: 5895 mean_circ: 0.906884
Ran for 649050 frames Cell Events: 5899 mean_circ: 0.906924
Ran for 649550 frames Cell Events: 5905 mean_circ: 0.906929
Ran for 650050 frames Cell Events: 5905 mean_circ: 0.906929
Ran for 650550 frames Cell Events: 5909 mean_circ: 0.906897
Ran for 651050 frames Cell Events: 5915 mean_circ: 0.906921
Ran for 651550 frames Cell Events: 5921 mean_circ: 0.906863
Ran for 652050 frames Cell Events: 5929 mean_circ: 0.906787
Ran for 652550 frames Cell Events: 5934 mean_circ: 0.906779
Ran for 653050 frames Cell Events: 5936 mean_circ: 0.906785
Ran for 653550 frames Cell Events: 5938 mean_circ: 0.906797
Ran for 654050 frames Cell Events: 5939 mean_circ: 0.906799
Ran for 654550 frames Cell Events: 5942 mean_circ: 0.906776
Ran for 655050 frames Cell Events: 5950 mean_circ: 0.906773
Ran for 655550 frames Cell Events: 5958 mean_circ: 0.906795
Ran for 656050 frames Cell Events: 5968 mean_circ: 0.906778
Ran for 657050 frames Cell Events: 5972 mean_circ: 0.906798
Ran for 657550 frames Cell Events: 5972 mean_circ: 0.906809
Ran for 658050 frames Cell Events: 5977 mean_circ: 0.906767
Ran for 659050 frames Cell Events: 5984 mean_circ: 0.90673
Ran for 659550 frames Cell Events: 5986 mean_circ: 0.906737
Ran for 660050 frames Cell Events: 5990 mean_circ: 0.906759
Ran for 660550 frames Cell Events: 6000 mean_circ: 0.906725
Ran for 661050 frames Cell Events: 6002 mean_circ: 0.906743
Ran for 661550 frames Cell Events: 6007 mean_circ: 0.90676
Ran for 662050 frames Cell Events: 6011 mean_circ: 0.906754
Ran for 662550 frames Cell Events: 6016 mean_circ: 0.906743
Ran for 663050 frames Cell Events: 6018 mean_circ: 0.906729
Ran for 663550 frames Cell Events: 6026 mean_circ: 0.906714
Ran for 664050 frames Cell Events: 6031 mean_circ: 0.906714
Ran for 664550 frames Cell Events: 6036 mean_circ: 0.906694
Ran for 665050 frames Cell Events: 6042 mean_circ: 0.906681
Ran for 665550 frames Cell Events: 6048 mean_circ: 0.906695
Ran for 666050 frames Cell Events: 6053 mean_circ: 0.906698
Ran for 666550 frames Cell Events: 6056 mean_circ: 0.906696
Ran for 667050 frames Cell Events: 6059 mean_circ: 0.906687
Ran for 667550 frames Cell Events: 6068 mean_circ: 0.906687
Ran for 668550 frames Cell Events: 6101 mean_circ: 0.906762
Ran for 669550 frames Cell Events: 6117 mean_circ: 0.906736
Ran for 670050 frames Cell Events: 6121 mean_circ: 0.906723
Ran for 670550 frames Cell Events: 6127 mean_circ: 0.906739
Ran for 671050 frames Cell Events: 6129 mean_circ: 0.906749
Ran for 671550 frames Cell Events: 6133 mean_circ: 0.906758
Ran for 672550 frames Cell Events: 6148 mean_circ: 0.906692
Ran for 673050 frames Cell Events: 6150 mean_circ: 0.906694
Ran for 674050 frames Cell Events: 6161 mean_circ: 0.906666
Ran for 674550 frames Cell Events: 6163 mean_circ: 0.906667
Ran for 675050 frames Cell Events: 6168 mean_circ: 0.906667
Ran for 675550 frames Cell Events: 6177 mean_circ: 0.906646
Ran for 676050 frames Cell Events: 6183 mean_circ: 0.906656
Ran for 676550 frames Cell Events: 6186 mean_circ: 0.906666
Ran for 677050 frames Cell Events: 6186 mean_circ: 0.906666
Ran for 677550 frames Cell Events: 6194 mean_circ: 0.90668
Ran for 678550 frames Cell Events: 6200 mean_circ: 0.906709
Ran for 679050 frames Cell Events: 6204 mean_circ: 0.906735
Ran for 680550 frames Cell Events: 6216 mean_circ: 0.906777
Ran for 681050 frames Cell Events: 6218 mean_circ: 0.906781
Ran for 681550 frames Cell Events: 6224 mean_circ: 0.906765
Ran for 682050 frames Cell Events: 6227 mean_circ: 0.90677
Ran for 682550 frames Cell Events: 6228 mean_circ: 0.906754
Ran for 683050 frames Cell Events: 6233 mean_circ: 0.9067
Ran for 683550 frames Cell Events: 6233 mean_circ: 0.9067
Ran for 684050 frames Cell Events: 6237 mean_circ: 0.906693
Ran for 684550 frames Cell Events: 6241 mean_circ: 0.906685
Ran for 685050 frames Cell Events: 6246 mean_circ: 0.906696
Ran for 685550 frames Cell Events: 6252 mean_circ: 0.906702
Ran for 686050 frames Cell Events: 6257 mean_circ: 0.906704
Ran for 686550 frames Cell Events: 6260 mean_circ: 0.906722
Ran for 687050 frames Cell Events: 6264 mean_circ: 0.906702
Ran for 687550 frames Cell Events: 6269 mean_circ: 0.906723
Ran for 688050 frames Cell Events: 6274 mean_circ: 0.90673
Ran for 688550 frames Cell Events: 6276 mean_circ: 0.906741
Ran for 689050 frames Cell Events: 6277 mean_circ: 0.906746
Ran for 689550 frames Cell Events: 6280 mean_circ: 0.90675
Ran for 690050 frames Cell Events: 6286 mean_circ: 0.906745
Ran for 691550 frames Cell Events: 6298 mean_circ: 0.906764
Ran for 692050 frames Cell Events: 6301 mean_circ: 0.906763
Ran for 692550 frames Cell Events: 6304 mean_circ: 0.90678
Ran for 693050 frames Cell Events: 6309 mean_circ: 0.906784
Ran for 693550 frames Cell Events: 6317 mean_circ: 0.906781
Ran for 694050 frames Cell Events: 6320 mean_circ: 0.906773
Ran for 694550 frames Cell Events: 6328 mean_circ: 0.906793
Ran for 695550 frames Cell Events: 6339 mean_circ: 0.906758
Ran for 696050 frames Cell Events: 6343 mean_circ: 0.906741
Ran for 696550 frames Cell Events: 6343 mean_circ: 0.906741
Ran for 697550 frames Cell Events: 6354 mean_circ: 0.906738
Ran for 698050 frames Cell Events: 6358 mean_circ: 0.906752
Ran for 698550 frames Cell Events: 6364 mean_circ: 0.906695
Ran for 699050 frames Cell Events: 6366 mean_circ: 0.906703
Ran for 699550 frames Cell Events: 6370 mean_circ: 0.906718
Ran for 700550 frames Cell Events: 6381 mean_circ: 0.906755
Ran for 701050 frames Cell Events: 6385 mean_circ: 0.906702
Ran for 701550 frames Cell Events: 6387 mean_circ: 0.906711
Ran for 702050 frames Cell Events: 6393 mean_circ: 0.906696
Ran for 702550 frames Cell Events: 6395 mean_circ: 0.906702
Ran for 703050 frames Cell Events: 6399 mean_circ: 0.906704
Ran for 703550 frames Cell Events: 6402 mean_circ: 0.906703
Ran for 704550 frames Cell Events: 6411 mean_circ: 0.906715
Ran for 705050 frames Cell Events: 6413 mean_circ: 0.906731
Ran for 705550 frames Cell Events: 6416 mean_circ: 0.906747
Ran for 706050 frames Cell Events: 6423 mean_circ: 0.906748
Ran for 706550 frames Cell Events: 6427 mean_circ: 0.906765
Ran for 707050 frames Cell Events: 6430 mean_circ: 0.906759
Ran for 707550 frames Cell Events: 6431 mean_circ: 0.906737
Ran for 708050 frames Cell Events: 6441 mean_circ: 0.906679
Ran for 708550 frames Cell Events: 6447 mean_circ: 0.90666
Ran for 709050 frames Cell Events: 6456 mean_circ: 0.906631
Ran for 709550 frames Cell Events: 6458 mean_circ: 0.906645
Ran for 710050 frames Cell Events: 6463 mean_circ: 0.906647
Ran for 711050 frames Cell Events: 6470 mean_circ: 0.906678
Ran for 711550 frames Cell Events: 6477 mean_circ: 0.906683
Ran for 712550 frames Cell Events: 6480 mean_circ: 0.906674
Ran for 713050 frames Cell Events: 6480 mean_circ: 0.906659
Ran for 713550 frames Cell Events: 6487 mean_circ: 0.906631
Ran for 714050 frames Cell Events: 6495 mean_circ: 0.906621
Ran for 714550 frames Cell Events: 6497 mean_circ: 0.90663
Ran for 715050 frames Cell Events: 6499 mean_circ: 0.906638
Ran for 715550 frames Cell Events: 6501 mean_circ: 0.906628
Ran for 716050 frames Cell Events: 6505 mean_circ: 0.90662
Ran for 716550 frames Cell Events: 6511 mean_circ: 0.906606
Ran for 717050 frames Cell Events: 6513 mean_circ: 0.90659
Ran for 717550 frames Cell Events: 6520 mean_circ: 0.906586
Ran for 718050 frames Cell Events: 6525 mean_circ: 0.906581
Ran for 718550 frames Cell Events: 6526 mean_circ: 0.906585
Ran for 719050 frames Cell Events: 6528 mean_circ: 0.906588
Ran for 719550 frames Cell Events: 6532 mean_circ: 0.906578
Ran for 720050 frames Cell Events: 6535 mean_circ: 0.906581
Ran for 720550 frames Cell Events: 6543 mean_circ: 0.906591
Ran for 721050 frames Cell Events: 6547 mean_circ: 0.906579
Ran for 721550 frames Cell Events: 6553 mean_circ: 0.906566
Ran for 722050 frames Cell Events: 6558 mean_circ: 0.906576
Ran for 722550 frames Cell Events: 6565 mean_circ: 0.906558
Ran for 723050 frames Cell Events: 6567 mean_circ: 0.90655
Ran for 724050 frames Cell Events: 6575 mean_circ: 0.906578
Ran for 724550 frames Cell Events: 6577 mean_circ: 0.906591
Ran for 725550 frames Cell Events: 6584 mean_circ: 0.906625
Ran for 726550 frames Cell Events: 6586 mean_circ: 0.906632
Ran for 727050 frames Cell Events: 6589 mean_circ: 0.906636
Ran for 727550 frames Cell Events: 6596 mean_circ: 0.90662
Ran for 728050 frames Cell Events: 6600 mean_circ: 0.906611
Ran for 728550 frames Cell Events: 6602 mean_circ: 0.906619
Ran for 729050 frames Cell Events: 6603 mean_circ: 0.906617
Ran for 729550 frames Cell Events: 6607 mean_circ: 0.906622
Ran for 730550 frames Cell Events: 6616 mean_circ: 0.906629
Ran for 731050 frames Cell Events: 6623 mean_circ: 0.906614
Ran for 731550 frames Cell Events: 6625 mean_circ: 0.906617
Ran for 732050 frames Cell Events: 6627 mean_circ: 0.906608
Ran for 732550 frames Cell Events: 6633 mean_circ: 0.906583
Ran for 733550 frames Cell Events: 6638 mean_circ: 0.906603
Ran for 734050 frames Cell Events: 6640 mean_circ: 0.906606
Ran for 734550 frames Cell Events: 6647 mean_circ: 0.906613
Ran for 735050 frames Cell Events: 6650 mean_circ: 0.906618
Ran for 735550 frames Cell Events: 6656 mean_circ: 0.906568
Ran for 736050 frames Cell Events: 6658 mean_circ: 0.906564
Ran for 736550 frames Cell Events: 6662 mean_circ: 0.906572
Ran for 737050 frames Cell Events: 6665 mean_circ: 0.906571
Ran for 737550 frames Cell Events: 6666 mean_circ: 0.906571
Ran for 738050 frames Cell Events: 6667 mean_circ: 0.906569
Ran for 738550 frames Cell Events: 6671 mean_circ: 0.906545
Ran for 739050 frames Cell Events: 6678 mean_circ: 0.906544
Ran for 739550 frames Cell Events: 6683 mean_circ: 0.906559
End this measurment..
Stopping time: 2.44069e+009
****************************************
Measurement finished
Mean Circularitiy: 0.906565
Mean Area: 1385.5
Mean FL-1 intensity: 6333.73
Mean FL-2 intensity: 7888.05
Mean FL-3 intensity: 0
frames processed: 736996
Total Time: 369897ms
Number of Runs (new bg img) 0
Frames collected: 739658
Skipped frames: 8882
Rescued Frames: 1319774
Samples DAQ: 184914449
Samples written: 369828784
Samples per Channel: 184914392
FPS: 1999.63
Samples per second: 499907
Expected samples/s: 500000
Events: 6684
Events per second: 18.0699
jumped back (PMT): 11
#Frames read, frequency
# 0 frames :904290
# 1 frames :543960
# 2 frames :7913
# 3 frames :5812
# 4 frames :1730
# 5 frames :1622
# 6 frames :770
# 7 frames :1955
# 8 frames :945
# 9 frames :922
# 10 frames :4243
# 11 frames :1846
# 12 frames :1080
# 13 frames :712
# 14 frames :322
# 15 frames :225
# 16 frames :234
# 17 frames :146
# 18 frames :126
# 19 frames :126
# 20 frames :86
# 21 frames :56
# 22 frames :48
# 23 frames :47
# 24 frames :23
# 25 frames :19
# 26 frames :2
# 27 frames :8
# 28 frames :2
# 29 frames :4
# 30 frames :4
# 31 frames :4
# 33 frames :1
# 40 frames :1
# 41 frames :1
# 53 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6912
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 40174e8c3e9c6bb90fde3d9a50a36c4e |
format | RT-FDC |
id | 7987e0b8-0691-4f0c-88b3-53b50c60160c |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | 7acae064-7ce7-4bfc-ac9b-9a87e4c5187c |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/798/7e0/b8-0691-4f0c-88b3-53b50c60160c |
sha256 | 148f0971e3bc95b008dfe720549fccf156a856785db05618df9505e7cfef6478 |
size | 118.1 MiB |
dc:experiment:date | 2017-06-12 |
dc:experiment:event count | 6,683 |
dc:experiment:run identifier | None-a64b |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170612_1415_dsRNA_S3_faster |
dc:experiment:time | 14:15:14 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 200 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.06 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |