Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.37.19
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2cf5b6654ae84fb1bf10c33652c762dc",
"name": "M1_20170612_1456_dsRNA_S7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:37:39"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8039 (max 8040) in '8039'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7383
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.44282e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 14 mean_circ: 0.931831
Ran for 1050 frames Cell Events: 34 mean_circ: 0.928633
Ran for 1550 frames Cell Events: 46 mean_circ: 0.933132
Ran for 2550 frames Cell Events: 74 mean_circ: 0.939484
Ran for 3050 frames Cell Events: 89 mean_circ: 0.939357
Ran for 3550 frames Cell Events: 106 mean_circ: 0.94041
Ran for 4550 frames Cell Events: 134 mean_circ: 0.937707
Ran for 5050 frames Cell Events: 148 mean_circ: 0.936717
Ran for 5550 frames Cell Events: 168 mean_circ: 0.934178
Ran for 7550 frames Cell Events: 231 mean_circ: 0.934844
Ran for 8050 frames Cell Events: 242 mean_circ: 0.934295
Ran for 9050 frames Cell Events: 274 mean_circ: 0.936072
Ran for 10050 frames Cell Events: 301 mean_circ: 0.936077
Ran for 11550 frames Cell Events: 360 mean_circ: 0.936471
Ran for 12050 frames Cell Events: 373 mean_circ: 0.935902
Ran for 12550 frames Cell Events: 388 mean_circ: 0.935906
Ran for 13050 frames Cell Events: 406 mean_circ: 0.935792
Ran for 14550 frames Cell Events: 468 mean_circ: 0.934571
Ran for 15050 frames Cell Events: 481 mean_circ: 0.934521
Ran for 16050 frames Cell Events: 510 mean_circ: 0.934948
Ran for 16550 frames Cell Events: 525 mean_circ: 0.935035
Ran for 19050 frames Cell Events: 615 mean_circ: 0.935606
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Ran for 69050 frames Cell Events: 2485 mean_circ: 0.937101
Ran for 69550 frames Cell Events: 2496 mean_circ: 0.936919
Ran for 70050 frames Cell Events: 2508 mean_circ: 0.936878
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Ran for 71550 frames Cell Events: 2560 mean_circ: 0.936744
Ran for 72050 frames Cell Events: 2570 mean_circ: 0.936733
Ran for 72550 frames Cell Events: 2598 mean_circ: 0.936806
Ran for 73550 frames Cell Events: 2637 mean_circ: 0.936706
Ran for 74050 frames Cell Events: 2650 mean_circ: 0.936686
Ran for 74550 frames Cell Events: 2662 mean_circ: 0.936659
Ran for 75550 frames Cell Events: 2695 mean_circ: 0.936593
Ran for 77550 frames Cell Events: 2786 mean_circ: 0.93672
Ran for 78050 frames Cell Events: 2803 mean_circ: 0.936822
Ran for 78550 frames Cell Events: 2819 mean_circ: 0.936868
Ran for 79050 frames Cell Events: 2842 mean_circ: 0.936784
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Ran for 81550 frames Cell Events: 2927 mean_circ: 0.936824
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Ran for 82550 frames Cell Events: 2972 mean_circ: 0.936851
Ran for 83050 frames Cell Events: 2990 mean_circ: 0.936817
Ran for 83550 frames Cell Events: 3008 mean_circ: 0.936661
Ran for 84050 frames Cell Events: 3023 mean_circ: 0.936732
Ran for 84550 frames Cell Events: 3047 mean_circ: 0.93678
Ran for 85050 frames Cell Events: 3068 mean_circ: 0.936833
Ran for 86550 frames Cell Events: 3138 mean_circ: 0.936972
Ran for 87050 frames Cell Events: 3149 mean_circ: 0.936988
Ran for 87550 frames Cell Events: 3168 mean_circ: 0.937047
Ran for 88050 frames Cell Events: 3189 mean_circ: 0.937009
Ran for 89550 frames Cell Events: 3251 mean_circ: 0.936897
Ran for 90050 frames Cell Events: 3278 mean_circ: 0.936704
Ran for 90550 frames Cell Events: 3293 mean_circ: 0.936646
Ran for 91050 frames Cell Events: 3307 mean_circ: 0.936662
Ran for 92050 frames Cell Events: 3352 mean_circ: 0.936583
Ran for 92550 frames Cell Events: 3380 mean_circ: 0.936477
Ran for 93050 frames Cell Events: 3402 mean_circ: 0.936412
Ran for 93550 frames Cell Events: 3417 mean_circ: 0.936409
Ran for 94050 frames Cell Events: 3435 mean_circ: 0.936381
Ran for 94550 frames Cell Events: 3451 mean_circ: 0.936357
Ran for 95050 frames Cell Events: 3465 mean_circ: 0.936407
Ran for 95550 frames Cell Events: 3489 mean_circ: 0.936279
Ran for 96550 frames Cell Events: 3534 mean_circ: 0.936217
Ran for 97550 frames Cell Events: 3565 mean_circ: 0.93628
Ran for 98550 frames Cell Events: 3606 mean_circ: 0.936315
Ran for 99050 frames Cell Events: 3629 mean_circ: 0.936381
Ran for 100050 frames Cell Events: 3667 mean_circ: 0.936434
Ran for 100550 frames Cell Events: 3678 mean_circ: 0.936451
Ran for 101050 frames Cell Events: 3695 mean_circ: 0.936345
Ran for 103050 frames Cell Events: 3769 mean_circ: 0.936502
Ran for 103550 frames Cell Events: 3780 mean_circ: 0.936489
Ran for 104050 frames Cell Events: 3797 mean_circ: 0.936501
Ran for 104550 frames Cell Events: 3809 mean_circ: 0.936507
Ran for 106050 frames Cell Events: 3858 mean_circ: 0.936519
Ran for 106550 frames Cell Events: 3887 mean_circ: 0.936522
Ran for 107050 frames Cell Events: 3902 mean_circ: 0.936546
Ran for 107550 frames Cell Events: 3928 mean_circ: 0.936611
Ran for 108050 frames Cell Events: 3947 mean_circ: 0.936641
Ran for 108550 frames Cell Events: 3974 mean_circ: 0.936643
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Ran for 117550 frames Cell Events: 4307 mean_circ: 0.936427
Ran for 118050 frames Cell Events: 4315 mean_circ: 0.936372
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Ran for 119550 frames Cell Events: 4352 mean_circ: 0.936479
Ran for 121050 frames Cell Events: 4375 mean_circ: 0.936488
Ran for 122050 frames Cell Events: 4415 mean_circ: 0.936465
Ran for 122550 frames Cell Events: 4433 mean_circ: 0.93648
Ran for 123050 frames Cell Events: 4442 mean_circ: 0.936513
Ran for 123550 frames Cell Events: 4449 mean_circ: 0.93652
Ran for 124050 frames Cell Events: 4456 mean_circ: 0.936522
Ran for 125050 frames Cell Events: 4491 mean_circ: 0.936514
Ran for 126050 frames Cell Events: 4528 mean_circ: 0.936637
Ran for 126550 frames Cell Events: 4547 mean_circ: 0.936586
Ran for 127050 frames Cell Events: 4557 mean_circ: 0.936642
Ran for 128050 frames Cell Events: 4590 mean_circ: 0.936675
Ran for 128550 frames Cell Events: 4605 mean_circ: 0.9367
Ran for 129050 frames Cell Events: 4613 mean_circ: 0.93671
Ran for 130050 frames Cell Events: 4644 mean_circ: 0.936629
Ran for 130550 frames Cell Events: 4652 mean_circ: 0.936607
Ran for 131550 frames Cell Events: 4697 mean_circ: 0.936599
Ran for 132550 frames Cell Events: 4734 mean_circ: 0.936563
Ran for 135050 frames Cell Events: 4817 mean_circ: 0.936525
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Ran for 136050 frames Cell Events: 4849 mean_circ: 0.936507
Ran for 136550 frames Cell Events: 4860 mean_circ: 0.936559
Ran for 137550 frames Cell Events: 4893 mean_circ: 0.936492
Ran for 138050 frames Cell Events: 4910 mean_circ: 0.936498
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Ran for 140550 frames Cell Events: 4989 mean_circ: 0.936432
Ran for 141050 frames Cell Events: 4997 mean_circ: 0.93638
Ran for 141550 frames Cell Events: 5006 mean_circ: 0.93636
Ran for 142050 frames Cell Events: 5016 mean_circ: 0.93633
Ran for 142550 frames Cell Events: 5036 mean_circ: 0.936341
Ran for 143050 frames Cell Events: 5049 mean_circ: 0.936318
Ran for 143550 frames Cell Events: 5067 mean_circ: 0.936308
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Ran for 146550 frames Cell Events: 5183 mean_circ: 0.936058
Ran for 147050 frames Cell Events: 5211 mean_circ: 0.936084
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Ran for 148050 frames Cell Events: 5242 mean_circ: 0.935984
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Ran for 167050 frames Cell Events: 5929 mean_circ: 0.935466
Ran for 167550 frames Cell Events: 5951 mean_circ: 0.935525
Ran for 168050 frames Cell Events: 5972 mean_circ: 0.935548
Ran for 169050 frames Cell Events: 6004 mean_circ: 0.935532
Ran for 170550 frames Cell Events: 6058 mean_circ: 0.935598
Ran for 171050 frames Cell Events: 6075 mean_circ: 0.935613
Ran for 171550 frames Cell Events: 6097 mean_circ: 0.935636
Ran for 172050 frames Cell Events: 6119 mean_circ: 0.935607
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Ran for 174550 frames Cell Events: 6204 mean_circ: 0.935668
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Ran for 176550 frames Cell Events: 6269 mean_circ: 0.935684
Ran for 177050 frames Cell Events: 6288 mean_circ: 0.935676
Ran for 178050 frames Cell Events: 6319 mean_circ: 0.935716
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Ran for 181050 frames Cell Events: 6421 mean_circ: 0.935756
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Ran for 190050 frames Cell Events: 6772 mean_circ: 0.935742
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Ran for 191050 frames Cell Events: 6801 mean_circ: 0.935763
Ran for 192050 frames Cell Events: 6843 mean_circ: 0.935795
Ran for 192550 frames Cell Events: 6860 mean_circ: 0.935782
Ran for 194050 frames Cell Events: 6918 mean_circ: 0.935791
Ran for 194550 frames Cell Events: 6936 mean_circ: 0.935784
Ran for 195050 frames Cell Events: 6951 mean_circ: 0.935811
Ran for 195550 frames Cell Events: 6969 mean_circ: 0.935796
Ran for 196050 frames Cell Events: 6989 mean_circ: 0.935802
Ran for 196550 frames Cell Events: 7003 mean_circ: 0.935789
Ran for 197050 frames Cell Events: 7018 mean_circ: 0.935792
Ran for 197550 frames Cell Events: 7038 mean_circ: 0.935783
Ran for 198550 frames Cell Events: 7078 mean_circ: 0.935736
Ran for 199050 frames Cell Events: 7094 mean_circ: 0.935738
Ran for 199550 frames Cell Events: 7110 mean_circ: 0.935741
Ran for 200550 frames Cell Events: 7156 mean_circ: 0.935758
Ran for 201050 frames Cell Events: 7188 mean_circ: 0.935761
Ran for 201550 frames Cell Events: 7205 mean_circ: 0.935785
Ran for 202050 frames Cell Events: 7222 mean_circ: 0.935812
Ran for 202550 frames Cell Events: 7244 mean_circ: 0.935786
Ran for 203050 frames Cell Events: 7264 mean_circ: 0.935787
Ran for 204550 frames Cell Events: 7338 mean_circ: 0.93577
Ran for 205550 frames Cell Events: 7374 mean_circ: 0.93574
Ran for 206050 frames Cell Events: 7391 mean_circ: 0.935768
Ran for 208050 frames Cell Events: 7464 mean_circ: 0.935781
Ran for 208550 frames Cell Events: 7476 mean_circ: 0.935797
Ran for 209050 frames Cell Events: 7490 mean_circ: 0.935829
Ran for 209550 frames Cell Events: 7509 mean_circ: 0.935815
Ran for 210550 frames Cell Events: 7555 mean_circ: 0.935856
Ran for 211050 frames Cell Events: 7576 mean_circ: 0.935827
Ran for 212050 frames Cell Events: 7619 mean_circ: 0.935862
Ran for 213050 frames Cell Events: 7667 mean_circ: 0.935834
Ran for 213550 frames Cell Events: 7688 mean_circ: 0.935835
Ran for 214050 frames Cell Events: 7710 mean_circ: 0.93572
Ran for 214550 frames Cell Events: 7726 mean_circ: 0.935738
Ran for 216050 frames Cell Events: 7772 mean_circ: 0.93573
Ran for 216550 frames Cell Events: 7785 mean_circ: 0.935729
Ran for 217050 frames Cell Events: 7805 mean_circ: 0.935747
Ran for 217550 frames Cell Events: 7827 mean_circ: 0.935714
Ran for 218050 frames Cell Events: 7843 mean_circ: 0.935773
Ran for 218550 frames Cell Events: 7867 mean_circ: 0.935791
Ran for 219050 frames Cell Events: 7879 mean_circ: 0.935791
Ran for 219550 frames Cell Events: 7907 mean_circ: 0.935802
Ran for 221050 frames Cell Events: 7965 mean_circ: 0.935841
Ran for 222050 frames Cell Events: 7997 mean_circ: 0.935815
Ran for 223050 frames Cell Events: 8037 mean_circ: 0.935766
End this measurment..
Stopping time: 2.44293e+009
****************************************
Measurement finished
Mean Circularitiy: 0.935775
Mean Area: 611.209
Mean FL-1 intensity: 3039.45
Mean FL-2 intensity: 3913.77
Mean FL-3 intensity: 0
frames processed: 221577
Total Time: 111632ms
Number of Runs (new bg img) 0
Frames collected: 223143
Skipped frames: 24875
Rescued Frames: 1827259
Samples DAQ: 55787694
Samples written: 111571542
Samples per Channel: 55785771
FPS: 1998.91
Samples per second: 499728
Expected samples/s: 500000
Events: 8040
Events per second: 72.0221
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :315619
# 1 frames :113584
# 2 frames :4811
# 3 frames :1740
# 4 frames :936
# 5 frames :422
# 6 frames :2170
# 7 frames :1488
# 8 frames :1114
# 9 frames :604
# 10 frames :1347
# 11 frames :497
# 12 frames :424
# 13 frames :229
# 14 frames :200
# 15 frames :151
# 16 frames :142
# 17 frames :138
# 18 frames :106
# 19 frames :74
# 20 frames :62
# 21 frames :56
# 22 frames :46
# 23 frames :36
# 24 frames :30
# 25 frames :25
# 26 frames :20
# 27 frames :12
# 28 frames :16
# 29 frames :11
# 30 frames :12
# 31 frames :14
# 32 frames :7
# 33 frames :7
# 34 frames :2
# 35 frames :6
# 36 frames :3
# 37 frames :3
# 38 frames :1
# 39 frames :2
# 41 frames :2
# 42 frames :3
# 44 frames :3
# 50 frames :2
# 51 frames :2
# 53 frames :2
# 61 frames :1
# 62 frames :1
# 67 frames :1
# 74 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7383
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0