Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.42.50
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ec74ab8278a16abbbd8cb1f15db383d0",
"name": "M1_20170612_1611_dsRNA_R1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:43:11"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8031 (max 8032) in '8031'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7703
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.44732e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 32 mean_circ: 0.945534
Ran for 1550 frames Cell Events: 38 mean_circ: 0.945957
Ran for 2050 frames Cell Events: 54 mean_circ: 0.947065
Ran for 3550 frames Cell Events: 92 mean_circ: 0.944163
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Ran for 64050 frames Cell Events: 1964 mean_circ: 0.940407
Ran for 64550 frames Cell Events: 1980 mean_circ: 0.94044
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Ran for 84550 frames Cell Events: 2564 mean_circ: 0.939587
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Ran for 85550 frames Cell Events: 2587 mean_circ: 0.93954
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Ran for 86550 frames Cell Events: 2624 mean_circ: 0.939563
Ran for 87050 frames Cell Events: 2637 mean_circ: 0.939622
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Ran for 92050 frames Cell Events: 2794 mean_circ: 0.939304
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Ran for 94050 frames Cell Events: 2831 mean_circ: 0.939327
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Ran for 103550 frames Cell Events: 3134 mean_circ: 0.939463
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Ran for 116050 frames Cell Events: 3535 mean_circ: 0.939417
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Ran for 119550 frames Cell Events: 3646 mean_circ: 0.939014
Ran for 120050 frames Cell Events: 3660 mean_circ: 0.938818
Ran for 120550 frames Cell Events: 3678 mean_circ: 0.938754
Ran for 121050 frames Cell Events: 3693 mean_circ: 0.938733
Ran for 122050 frames Cell Events: 3734 mean_circ: 0.938557
Ran for 123050 frames Cell Events: 3777 mean_circ: 0.938634
Ran for 123550 frames Cell Events: 3791 mean_circ: 0.938613
Ran for 124050 frames Cell Events: 3807 mean_circ: 0.938534
Ran for 124550 frames Cell Events: 3828 mean_circ: 0.938435
Ran for 125050 frames Cell Events: 3842 mean_circ: 0.938487
Ran for 125550 frames Cell Events: 3858 mean_circ: 0.938428
Ran for 126050 frames Cell Events: 3877 mean_circ: 0.938403
Ran for 127050 frames Cell Events: 3911 mean_circ: 0.938418
Ran for 127550 frames Cell Events: 3928 mean_circ: 0.938488
Ran for 128050 frames Cell Events: 3949 mean_circ: 0.938511
Ran for 128550 frames Cell Events: 3967 mean_circ: 0.93854
Ran for 129050 frames Cell Events: 3984 mean_circ: 0.938632
Ran for 129550 frames Cell Events: 4001 mean_circ: 0.938621
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Ran for 131550 frames Cell Events: 4062 mean_circ: 0.93868
Ran for 132050 frames Cell Events: 4075 mean_circ: 0.93868
Ran for 132550 frames Cell Events: 4084 mean_circ: 0.938628
Ran for 133050 frames Cell Events: 4099 mean_circ: 0.938686
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Ran for 142050 frames Cell Events: 4380 mean_circ: 0.938874
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Ran for 172550 frames Cell Events: 5293 mean_circ: 0.938876
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Ran for 182050 frames Cell Events: 5568 mean_circ: 0.938874
Ran for 182550 frames Cell Events: 5586 mean_circ: 0.938873
Ran for 183050 frames Cell Events: 5600 mean_circ: 0.938867
Ran for 183550 frames Cell Events: 5609 mean_circ: 0.938897
Ran for 184050 frames Cell Events: 5618 mean_circ: 0.938907
Ran for 184550 frames Cell Events: 5634 mean_circ: 0.938928
Ran for 185050 frames Cell Events: 5644 mean_circ: 0.938939
Ran for 186050 frames Cell Events: 5670 mean_circ: 0.938977
Ran for 186550 frames Cell Events: 5683 mean_circ: 0.938908
Ran for 187550 frames Cell Events: 5721 mean_circ: 0.938934
Ran for 188050 frames Cell Events: 5744 mean_circ: 0.93892
Ran for 188550 frames Cell Events: 5755 mean_circ: 0.938925
Ran for 189050 frames Cell Events: 5761 mean_circ: 0.938897
Ran for 189550 frames Cell Events: 5772 mean_circ: 0.938939
Ran for 190050 frames Cell Events: 5789 mean_circ: 0.938933
Ran for 190550 frames Cell Events: 5800 mean_circ: 0.938912
Ran for 191050 frames Cell Events: 5811 mean_circ: 0.938923
Ran for 192050 frames Cell Events: 5833 mean_circ: 0.938901
Ran for 192550 frames Cell Events: 5845 mean_circ: 0.938886
Ran for 193050 frames Cell Events: 5869 mean_circ: 0.938863
Ran for 193550 frames Cell Events: 5887 mean_circ: 0.938838
Ran for 194050 frames Cell Events: 5917 mean_circ: 0.938884
Ran for 194550 frames Cell Events: 5935 mean_circ: 0.93889
Ran for 195050 frames Cell Events: 5952 mean_circ: 0.938861
Ran for 196050 frames Cell Events: 5990 mean_circ: 0.938823
Ran for 196550 frames Cell Events: 6007 mean_circ: 0.938783
Ran for 197050 frames Cell Events: 6018 mean_circ: 0.938782
Ran for 197550 frames Cell Events: 6034 mean_circ: 0.938741
Ran for 198050 frames Cell Events: 6051 mean_circ: 0.938735
Ran for 199550 frames Cell Events: 6091 mean_circ: 0.938678
Ran for 200050 frames Cell Events: 6111 mean_circ: 0.938579
Ran for 201050 frames Cell Events: 6138 mean_circ: 0.938583
Ran for 202050 frames Cell Events: 6163 mean_circ: 0.938573
Ran for 203050 frames Cell Events: 6198 mean_circ: 0.938547
Ran for 204050 frames Cell Events: 6240 mean_circ: 0.93857
Ran for 204550 frames Cell Events: 6261 mean_circ: 0.938603
Ran for 205050 frames Cell Events: 6275 mean_circ: 0.938634
Ran for 205550 frames Cell Events: 6290 mean_circ: 0.938645
Ran for 206050 frames Cell Events: 6309 mean_circ: 0.938624
Ran for 206550 frames Cell Events: 6322 mean_circ: 0.938604
Ran for 207050 frames Cell Events: 6334 mean_circ: 0.938604
Ran for 207550 frames Cell Events: 6348 mean_circ: 0.938598
Ran for 208050 frames Cell Events: 6358 mean_circ: 0.938585
Ran for 209050 frames Cell Events: 6383 mean_circ: 0.938551
Ran for 209550 frames Cell Events: 6398 mean_circ: 0.938552
Ran for 210050 frames Cell Events: 6412 mean_circ: 0.938552
Ran for 211050 frames Cell Events: 6449 mean_circ: 0.938586
Ran for 212550 frames Cell Events: 6492 mean_circ: 0.938587
Ran for 213050 frames Cell Events: 6502 mean_circ: 0.938561
Ran for 213550 frames Cell Events: 6511 mean_circ: 0.938533
Ran for 214050 frames Cell Events: 6520 mean_circ: 0.938533
Ran for 215050 frames Cell Events: 6564 mean_circ: 0.9385
Ran for 216050 frames Cell Events: 6603 mean_circ: 0.938553
Ran for 216550 frames Cell Events: 6623 mean_circ: 0.938564
Ran for 217050 frames Cell Events: 6635 mean_circ: 0.938558
Ran for 217550 frames Cell Events: 6651 mean_circ: 0.938584
Ran for 218050 frames Cell Events: 6665 mean_circ: 0.93861
Ran for 218550 frames Cell Events: 6679 mean_circ: 0.93858
Ran for 219050 frames Cell Events: 6696 mean_circ: 0.938554
Ran for 219550 frames Cell Events: 6714 mean_circ: 0.93856
Ran for 220050 frames Cell Events: 6731 mean_circ: 0.938577
Ran for 221050 frames Cell Events: 6757 mean_circ: 0.938597
Ran for 222050 frames Cell Events: 6783 mean_circ: 0.938554
Ran for 222550 frames Cell Events: 6787 mean_circ: 0.938568
Ran for 223050 frames Cell Events: 6808 mean_circ: 0.938568
Ran for 223550 frames Cell Events: 6817 mean_circ: 0.938534
Ran for 224050 frames Cell Events: 6829 mean_circ: 0.938553
Ran for 225050 frames Cell Events: 6865 mean_circ: 0.938575
Ran for 225550 frames Cell Events: 6877 mean_circ: 0.93856
Ran for 226050 frames Cell Events: 6894 mean_circ: 0.938564
Ran for 226550 frames Cell Events: 6913 mean_circ: 0.938546
Ran for 227050 frames Cell Events: 6926 mean_circ: 0.938592
Ran for 228050 frames Cell Events: 6957 mean_circ: 0.938612
Ran for 228550 frames Cell Events: 6971 mean_circ: 0.938634
Ran for 229050 frames Cell Events: 6984 mean_circ: 0.938636
Ran for 229550 frames Cell Events: 6999 mean_circ: 0.938647
Ran for 230050 frames Cell Events: 7010 mean_circ: 0.938639
Ran for 231050 frames Cell Events: 7043 mean_circ: 0.938619
Ran for 232050 frames Cell Events: 7066 mean_circ: 0.938629
Ran for 233050 frames Cell Events: 7097 mean_circ: 0.938581
Ran for 233550 frames Cell Events: 7103 mean_circ: 0.938568
Ran for 234550 frames Cell Events: 7131 mean_circ: 0.9386
Ran for 235050 frames Cell Events: 7138 mean_circ: 0.938632
Ran for 235550 frames Cell Events: 7149 mean_circ: 0.93861
Ran for 236050 frames Cell Events: 7168 mean_circ: 0.938577
Ran for 236550 frames Cell Events: 7182 mean_circ: 0.938576
Ran for 237050 frames Cell Events: 7198 mean_circ: 0.938608
Ran for 237550 frames Cell Events: 7220 mean_circ: 0.938597
Ran for 238050 frames Cell Events: 7242 mean_circ: 0.938578
Ran for 239550 frames Cell Events: 7302 mean_circ: 0.938604
Ran for 240050 frames Cell Events: 7321 mean_circ: 0.938606
Ran for 240550 frames Cell Events: 7338 mean_circ: 0.938621
Ran for 242050 frames Cell Events: 7401 mean_circ: 0.938697
Ran for 243050 frames Cell Events: 7428 mean_circ: 0.938637
Ran for 244550 frames Cell Events: 7476 mean_circ: 0.938672
Ran for 245550 frames Cell Events: 7515 mean_circ: 0.938702
Ran for 246050 frames Cell Events: 7534 mean_circ: 0.93869
Ran for 246550 frames Cell Events: 7555 mean_circ: 0.938726
Ran for 248050 frames Cell Events: 7611 mean_circ: 0.938741
Ran for 249050 frames Cell Events: 7642 mean_circ: 0.938747
Ran for 249550 frames Cell Events: 7651 mean_circ: 0.938752
Ran for 250050 frames Cell Events: 7665 mean_circ: 0.938734
Ran for 251050 frames Cell Events: 7687 mean_circ: 0.938754
Ran for 252550 frames Cell Events: 7736 mean_circ: 0.938675
Ran for 253050 frames Cell Events: 7749 mean_circ: 0.938683
Ran for 253550 frames Cell Events: 7765 mean_circ: 0.938679
Ran for 254050 frames Cell Events: 7781 mean_circ: 0.938683
Ran for 255550 frames Cell Events: 7842 mean_circ: 0.938688
Ran for 256550 frames Cell Events: 7873 mean_circ: 0.938659
Ran for 257050 frames Cell Events: 7889 mean_circ: 0.938638
Ran for 257550 frames Cell Events: 7912 mean_circ: 0.93863
Ran for 258050 frames Cell Events: 7923 mean_circ: 0.938614
Ran for 258550 frames Cell Events: 7934 mean_circ: 0.938602
Ran for 259050 frames Cell Events: 7946 mean_circ: 0.938591
Ran for 260050 frames Cell Events: 7979 mean_circ: 0.938623
Ran for 260550 frames Cell Events: 7994 mean_circ: 0.938665
Ran for 261050 frames Cell Events: 8002 mean_circ: 0.938693
End this measurment..
Stopping time: 2.44745e+009
****************************************
Measurement finished
Mean Circularitiy: 0.938707
Mean Area: 631.553
Mean FL-1 intensity: 3088.24
Mean FL-2 intensity: 4161.52
Mean FL-3 intensity: 0
frames processed: 259642
Total Time: 130980ms
Number of Runs (new bg img) 0
Frames collected: 261823
Skipped frames: 2353
Rescued Frames: 208719
Samples DAQ: 65455679
Samples written: 130911306
Samples per Channel: 65455653
FPS: 1998.96
Samples per second: 499740
Expected samples/s: 500000
Events: 8032
Events per second: 61.3226
jumped back (PMT): 6
#Frames read, frequency
# 0 frames :352477
# 1 frames :152904
# 2 frames :5509
# 3 frames :1955
# 4 frames :873
# 5 frames :338
# 6 frames :2871
# 7 frames :1739
# 8 frames :1154
# 9 frames :639
# 10 frames :973
# 11 frames :597
# 12 frames :521
# 13 frames :236
# 14 frames :193
# 15 frames :149
# 16 frames :112
# 17 frames :88
# 18 frames :60
# 19 frames :55
# 20 frames :27
# 21 frames :30
# 22 frames :21
# 23 frames :16
# 24 frames :15
# 25 frames :8
# 26 frames :12
# 27 frames :6
# 28 frames :7
# 29 frames :1
# 30 frames :5
# 31 frames :3
# 32 frames :4
# 33 frames :2
# 34 frames :3
# 35 frames :2
# 36 frames :1
# 37 frames :2
# 38 frames :1
# 39 frames :2
# 42 frames :1
# 43 frames :1
# 46 frames :1
# 49 frames :1
# 50 frames :1
# 55 frames :1
# 79 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7703
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0