M1_20170612_1633_dsRNA_S1_col.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.44.11
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "beacf907fa468e00ffd76b0a4306b39a",
"name": "M1_20170612_1633_dsRNA_S1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:44:32"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarningin dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8013 (max 8014) in '8013'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7464
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.44862e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
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Ran for 564550 frames Cell Events: 6725 mean_circ: 0.945321
Ran for 565050 frames Cell Events: 6728 mean_circ: 0.9453
Ran for 565550 frames Cell Events: 6735 mean_circ: 0.945303
Ran for 566050 frames Cell Events: 6735 mean_circ: 0.945303
Ran for 566550 frames Cell Events: 6741 mean_circ: 0.945292
Ran for 567050 frames Cell Events: 6745 mean_circ: 0.945303
Ran for 567550 frames Cell Events: 6748 mean_circ: 0.945275
Ran for 568050 frames Cell Events: 6753 mean_circ: 0.945284
Ran for 568550 frames Cell Events: 6761 mean_circ: 0.94527
Ran for 569050 frames Cell Events: 6770 mean_circ: 0.945254
Ran for 570050 frames Cell Events: 6785 mean_circ: 0.945276
Ran for 570550 frames Cell Events: 6790 mean_circ: 0.945248
Ran for 571050 frames Cell Events: 6798 mean_circ: 0.945258
Ran for 572050 frames Cell Events: 6812 mean_circ: 0.945231
Ran for 572550 frames Cell Events: 6820 mean_circ: 0.945233
Ran for 573550 frames Cell Events: 6844 mean_circ: 0.945265
Ran for 574050 frames Cell Events: 6851 mean_circ: 0.94527
Ran for 574550 frames Cell Events: 6853 mean_circ: 0.945277
Ran for 575050 frames Cell Events: 6855 mean_circ: 0.945278
Ran for 575550 frames Cell Events: 6861 mean_circ: 0.945278
Ran for 576050 frames Cell Events: 6867 mean_circ: 0.945282
Ran for 576550 frames Cell Events: 6869 mean_circ: 0.945264
Ran for 577550 frames Cell Events: 6879 mean_circ: 0.945259
Ran for 578550 frames Cell Events: 6884 mean_circ: 0.945227
Ran for 579050 frames Cell Events: 6886 mean_circ: 0.945234
Ran for 580050 frames Cell Events: 6892 mean_circ: 0.945241
Ran for 580550 frames Cell Events: 6899 mean_circ: 0.945227
Ran for 581050 frames Cell Events: 6906 mean_circ: 0.945194
Ran for 581550 frames Cell Events: 6910 mean_circ: 0.945176
Ran for 582050 frames Cell Events: 6915 mean_circ: 0.945192
Ran for 582550 frames Cell Events: 6920 mean_circ: 0.945203
Ran for 583050 frames Cell Events: 6928 mean_circ: 0.945185
Ran for 583550 frames Cell Events: 6942 mean_circ: 0.945141
Ran for 584050 frames Cell Events: 6949 mean_circ: 0.945142
Ran for 584550 frames Cell Events: 6955 mean_circ: 0.94513
Ran for 585050 frames Cell Events: 6960 mean_circ: 0.945141
Ran for 585550 frames Cell Events: 6965 mean_circ: 0.94515
Ran for 586050 frames Cell Events: 6967 mean_circ: 0.945157
Ran for 586550 frames Cell Events: 6968 mean_circ: 0.945147
Ran for 588050 frames Cell Events: 6978 mean_circ: 0.945167
Ran for 588550 frames Cell Events: 6983 mean_circ: 0.945175
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Ran for 589550 frames Cell Events: 6989 mean_circ: 0.945188
Ran for 590050 frames Cell Events: 6995 mean_circ: 0.945156
Ran for 590550 frames Cell Events: 7002 mean_circ: 0.945159
Ran for 591050 frames Cell Events: 7012 mean_circ: 0.945154
Ran for 591550 frames Cell Events: 7017 mean_circ: 0.945156
Ran for 592050 frames Cell Events: 7022 mean_circ: 0.945157
Ran for 592550 frames Cell Events: 7029 mean_circ: 0.945131
Ran for 593550 frames Cell Events: 7040 mean_circ: 0.945106
Ran for 594050 frames Cell Events: 7046 mean_circ: 0.945113
Ran for 594550 frames Cell Events: 7054 mean_circ: 0.94512
Ran for 595050 frames Cell Events: 7056 mean_circ: 0.9451
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Ran for 596550 frames Cell Events: 7073 mean_circ: 0.9451
Ran for 597050 frames Cell Events: 7083 mean_circ: 0.945119
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Ran for 598550 frames Cell Events: 7097 mean_circ: 0.94509
Ran for 599050 frames Cell Events: 7099 mean_circ: 0.945092
Ran for 599550 frames Cell Events: 7100 mean_circ: 0.945096
Ran for 600550 frames Cell Events: 7116 mean_circ: 0.945086
Ran for 601050 frames Cell Events: 7122 mean_circ: 0.945072
Ran for 601550 frames Cell Events: 7128 mean_circ: 0.945068
Ran for 602050 frames Cell Events: 7134 mean_circ: 0.945073
Ran for 602550 frames Cell Events: 7139 mean_circ: 0.945067
Ran for 603050 frames Cell Events: 7144 mean_circ: 0.945072
Ran for 604050 frames Cell Events: 7152 mean_circ: 0.945069
Ran for 604550 frames Cell Events: 7159 mean_circ: 0.945073
Ran for 605050 frames Cell Events: 7163 mean_circ: 0.945084
Ran for 605550 frames Cell Events: 7165 mean_circ: 0.945091
Ran for 606050 frames Cell Events: 7170 mean_circ: 0.945109
Ran for 606550 frames Cell Events: 7173 mean_circ: 0.945117
Ran for 607050 frames Cell Events: 7173 mean_circ: 0.945118
Ran for 608050 frames Cell Events: 7188 mean_circ: 0.945121
Ran for 608550 frames Cell Events: 7192 mean_circ: 0.945119
Ran for 609050 frames Cell Events: 7199 mean_circ: 0.945117
Ran for 610050 frames Cell Events: 7216 mean_circ: 0.94512
Ran for 610550 frames Cell Events: 7224 mean_circ: 0.945109
Ran for 611050 frames Cell Events: 7229 mean_circ: 0.945115
Ran for 611550 frames Cell Events: 7238 mean_circ: 0.945129
Ran for 612050 frames Cell Events: 7245 mean_circ: 0.945135
Ran for 612550 frames Cell Events: 7248 mean_circ: 0.945123
Ran for 613050 frames Cell Events: 7249 mean_circ: 0.945127
Ran for 613550 frames Cell Events: 7255 mean_circ: 0.945134
Ran for 614050 frames Cell Events: 7259 mean_circ: 0.945121
Ran for 615050 frames Cell Events: 7273 mean_circ: 0.945107
Ran for 615550 frames Cell Events: 7282 mean_circ: 0.945111
Ran for 616050 frames Cell Events: 7286 mean_circ: 0.94512
Ran for 616550 frames Cell Events: 7301 mean_circ: 0.945099
Ran for 617050 frames Cell Events: 7313 mean_circ: 0.945088
Ran for 617550 frames Cell Events: 7314 mean_circ: 0.945081
Ran for 618050 frames Cell Events: 7317 mean_circ: 0.94507
Ran for 618550 frames Cell Events: 7320 mean_circ: 0.945064
Ran for 619050 frames Cell Events: 7327 mean_circ: 0.945064
Ran for 619550 frames Cell Events: 7329 mean_circ: 0.945081
Ran for 620050 frames Cell Events: 7331 mean_circ: 0.945075
Ran for 620550 frames Cell Events: 7336 mean_circ: 0.945081
Ran for 621050 frames Cell Events: 7339 mean_circ: 0.94508
Ran for 621550 frames Cell Events: 7348 mean_circ: 0.945073
Ran for 622050 frames Cell Events: 7352 mean_circ: 0.945045
Ran for 622550 frames Cell Events: 7355 mean_circ: 0.945035
Ran for 623050 frames Cell Events: 7362 mean_circ: 0.945035
Ran for 623550 frames Cell Events: 7365 mean_circ: 0.945004
Ran for 624050 frames Cell Events: 7374 mean_circ: 0.94502
Ran for 624550 frames Cell Events: 7379 mean_circ: 0.944982
Ran for 625550 frames Cell Events: 7389 mean_circ: 0.945008
Ran for 626050 frames Cell Events: 7394 mean_circ: 0.945018
Ran for 626550 frames Cell Events: 7396 mean_circ: 0.945027
Ran for 627050 frames Cell Events: 7403 mean_circ: 0.945022
Ran for 627550 frames Cell Events: 7407 mean_circ: 0.945008
Ran for 628550 frames Cell Events: 7421 mean_circ: 0.945027
Ran for 629050 frames Cell Events: 7426 mean_circ: 0.945028
Ran for 629550 frames Cell Events: 7431 mean_circ: 0.945029
Ran for 630050 frames Cell Events: 7437 mean_circ: 0.945048
Ran for 631050 frames Cell Events: 7448 mean_circ: 0.945053
Ran for 631550 frames Cell Events: 7453 mean_circ: 0.945063
Ran for 632050 frames Cell Events: 7460 mean_circ: 0.945083
Ran for 632550 frames Cell Events: 7462 mean_circ: 0.945085
Ran for 633050 frames Cell Events: 7466 mean_circ: 0.945095
Ran for 634050 frames Cell Events: 7476 mean_circ: 0.945105
Ran for 634550 frames Cell Events: 7478 mean_circ: 0.94511
Ran for 635050 frames Cell Events: 7479 mean_circ: 0.945111
Ran for 635550 frames Cell Events: 7482 mean_circ: 0.945105
Ran for 636050 frames Cell Events: 7487 mean_circ: 0.945119
Ran for 636550 frames Cell Events: 7490 mean_circ: 0.945122
Ran for 637550 frames Cell Events: 7497 mean_circ: 0.945134
Ran for 638050 frames Cell Events: 7498 mean_circ: 0.945137
Ran for 638550 frames Cell Events: 7505 mean_circ: 0.945131
Ran for 639050 frames Cell Events: 7509 mean_circ: 0.945107
Ran for 639550 frames Cell Events: 7513 mean_circ: 0.945099
Ran for 640550 frames Cell Events: 7524 mean_circ: 0.945103
Ran for 641550 frames Cell Events: 7539 mean_circ: 0.945105
Ran for 642050 frames Cell Events: 7543 mean_circ: 0.945076
Ran for 642550 frames Cell Events: 7548 mean_circ: 0.94509
Ran for 643050 frames Cell Events: 7552 mean_circ: 0.945099
Ran for 643550 frames Cell Events: 7556 mean_circ: 0.94511
Ran for 644050 frames Cell Events: 7558 mean_circ: 0.945111
Ran for 644550 frames Cell Events: 7565 mean_circ: 0.945119
Ran for 645050 frames Cell Events: 7568 mean_circ: 0.945122
Ran for 645550 frames Cell Events: 7577 mean_circ: 0.945127
Ran for 646050 frames Cell Events: 7581 mean_circ: 0.945136
Ran for 647050 frames Cell Events: 7597 mean_circ: 0.945142
Ran for 647550 frames Cell Events: 7597 mean_circ: 0.945142
Ran for 648050 frames Cell Events: 7600 mean_circ: 0.945135
Ran for 648550 frames Cell Events: 7604 mean_circ: 0.945129
Ran for 649050 frames Cell Events: 7605 mean_circ: 0.945135
Ran for 649550 frames Cell Events: 7607 mean_circ: 0.945138
Ran for 650050 frames Cell Events: 7608 mean_circ: 0.945142
Ran for 650550 frames Cell Events: 7613 mean_circ: 0.945148
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Ran for 651550 frames Cell Events: 7620 mean_circ: 0.945151
Ran for 652050 frames Cell Events: 7625 mean_circ: 0.94516
Ran for 652550 frames Cell Events: 7630 mean_circ: 0.945164
Ran for 653550 frames Cell Events: 7637 mean_circ: 0.945138
Ran for 654050 frames Cell Events: 7638 mean_circ: 0.94514
Ran for 654550 frames Cell Events: 7638 mean_circ: 0.94514
Ran for 655050 frames Cell Events: 7643 mean_circ: 0.945156
Ran for 655550 frames Cell Events: 7650 mean_circ: 0.945177
Ran for 656050 frames Cell Events: 7653 mean_circ: 0.945183
Ran for 656550 frames Cell Events: 7662 mean_circ: 0.945179
Ran for 657050 frames Cell Events: 7669 mean_circ: 0.945192
Ran for 658050 frames Cell Events: 7677 mean_circ: 0.945205
Ran for 658550 frames Cell Events: 7686 mean_circ: 0.945227
Ran for 659050 frames Cell Events: 7690 mean_circ: 0.945232
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Ran for 660050 frames Cell Events: 7703 mean_circ: 0.945205
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Ran for 662050 frames Cell Events: 7738 mean_circ: 0.945215
Ran for 662550 frames Cell Events: 7740 mean_circ: 0.945206
Ran for 663050 frames Cell Events: 7743 mean_circ: 0.945212
Ran for 664050 frames Cell Events: 7750 mean_circ: 0.945207
Ran for 665050 frames Cell Events: 7760 mean_circ: 0.945227
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Ran for 666550 frames Cell Events: 7776 mean_circ: 0.945213
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Ran for 667550 frames Cell Events: 7789 mean_circ: 0.94522
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Ran for 672550 frames Cell Events: 7828 mean_circ: 0.945246
Ran for 673050 frames Cell Events: 7831 mean_circ: 0.945249
Ran for 673550 frames Cell Events: 7837 mean_circ: 0.945258
Ran for 674050 frames Cell Events: 7841 mean_circ: 0.945248
Ran for 674550 frames Cell Events: 7841 mean_circ: 0.945254
Ran for 675050 frames Cell Events: 7844 mean_circ: 0.945233
Ran for 675550 frames Cell Events: 7848 mean_circ: 0.945245
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Ran for 676550 frames Cell Events: 7854 mean_circ: 0.945234
Ran for 677050 frames Cell Events: 7860 mean_circ: 0.94524
Ran for 678050 frames Cell Events: 7870 mean_circ: 0.945248
Ran for 678550 frames Cell Events: 7876 mean_circ: 0.945244
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Ran for 680050 frames Cell Events: 7888 mean_circ: 0.945222
Ran for 680550 frames Cell Events: 7890 mean_circ: 0.94522
Ran for 681050 frames Cell Events: 7900 mean_circ: 0.945212
Ran for 681550 frames Cell Events: 7910 mean_circ: 0.945216
Ran for 682050 frames Cell Events: 7917 mean_circ: 0.945215
Ran for 682550 frames Cell Events: 7920 mean_circ: 0.945223
Ran for 683050 frames Cell Events: 7924 mean_circ: 0.94523
Ran for 684050 frames Cell Events: 7933 mean_circ: 0.945233
Ran for 684550 frames Cell Events: 7937 mean_circ: 0.945239
Ran for 685050 frames Cell Events: 7940 mean_circ: 0.945242
Ran for 685550 frames Cell Events: 7943 mean_circ: 0.945251
Ran for 686050 frames Cell Events: 7956 mean_circ: 0.945249
Ran for 686550 frames Cell Events: 7964 mean_circ: 0.945266
Ran for 687050 frames Cell Events: 7970 mean_circ: 0.945252
Ran for 687550 frames Cell Events: 7976 mean_circ: 0.945255
Ran for 688050 frames Cell Events: 7980 mean_circ: 0.945263
Ran for 688550 frames Cell Events: 7986 mean_circ: 0.94527
Ran for 689050 frames Cell Events: 7988 mean_circ: 0.945255
Ran for 689550 frames Cell Events: 7995 mean_circ: 0.945253
Ran for 690550 frames Cell Events: 8011 mean_circ: 0.945254
End this measurment..
Stopping time: 2.44896e+009
****************************************
Measurement finished
Mean Circularitiy: 0.945139
Mean Area: 620.292
Mean FL-1 intensity: 2610.98
Mean FL-2 intensity: 3277.15
Mean FL-3 intensity: 0
frames processed: 690435
Total Time: 345613ms
Number of Runs (new bg img) 0
Frames collected: 691046
Skipped frames: 4630
Rescued Frames: 580432
Samples DAQ: 172762677
Samples written: 345522758
Samples per Channel: 172761379
FPS: 1999.48
Samples per second: 499869
Expected samples/s: 500000
Events: 8015
Events per second: 23.1907
jumped back (PMT): 7710
#Frames read, frequency
# 0 frames :867395
# 1 frames :482334
# 2 frames :7924
# 3 frames :6119
# 4 frames :2635
# 5 frames :1287
# 6 frames :613
# 7 frames :1855
# 8 frames :1313
# 9 frames :1097
# 10 frames :1427
# 11 frames :2638
# 12 frames :1918
# 13 frames :1447
# 14 frames :604
# 15 frames :336
# 16 frames :200
# 17 frames :216
# 18 frames :162
# 19 frames :104
# 20 frames :95
# 21 frames :80
# 22 frames :43
# 23 frames :41
# 24 frames :27
# 25 frames :28
# 26 frames :12
# 27 frames :13
# 28 frames :9
# 29 frames :8
# 30 frames :9
# 31 frames :10
# 32 frames :6
# 33 frames :5
# 34 frames :3
# 35 frames :3
# 37 frames :1
# 38 frames :3
# 39 frames :2
# 40 frames :1
# 41 frames :1
# 44 frames :2
# 53 frames :1
# 56 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7464
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | fd66d2c5794587adb781e581fa7ecfdd |
format | RT-FDC |
id | cdfcff6c-e0fb-4862-99ae-3f0065382583 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | 448f5f86-b38c-449b-8d8d-a2526103d6e1 |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/cdf/cff/6c-e0fb-4862-99ae-3f0065382583 |
sha256 | e7f72f893444a15a84f41ecc3cd1eb43e53b345c11adafa7a007827946a1990d |
size | 140.2 MiB |
dc:experiment:date | 2017-06-12 |
dc:experiment:event count | 8,012 |
dc:experiment:run identifier | None-5ccf |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170612_1633_dsRNA_S1_col |
dc:experiment:time | 16:33:31 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |